BR0316525A - Genes de fosfotransferase de neomicina e processos para seleção de células recombinantes de alta produtividade - Google Patents

Genes de fosfotransferase de neomicina e processos para seleção de células recombinantes de alta produtividade

Info

Publication number
BR0316525A
BR0316525A BR0316525-6A BR0316525A BR0316525A BR 0316525 A BR0316525 A BR 0316525A BR 0316525 A BR0316525 A BR 0316525A BR 0316525 A BR0316525 A BR 0316525A
Authority
BR
Brazil
Prior art keywords
neomycin phosphotransferase
recombinant cells
processes
high yield
selecting high
Prior art date
Application number
BR0316525-6A
Other languages
English (en)
Inventor
Barbara Enenkel
Kerstin Sautter
Ralf Otto
Juergen Fieder
Klaus Bergemann
Original Assignee
Boehringer Ingelheim Pharma
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from DE10330686A external-priority patent/DE10330686A1/de
Application filed by Boehringer Ingelheim Pharma filed Critical Boehringer Ingelheim Pharma
Publication of BR0316525A publication Critical patent/BR0316525A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/65—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression using markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01095—Kanamycin kinase (2.7.1.95), i.e. neomycin-kanamycin phosphotransferase

Landscapes

  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"GENES DE FOSFOTRANSFERASE DE NEOMICINA E PROCESSOS PARA SELEçãO DE CéLULAS RECOMBINANTES DE ALTA PRODUTIVIDADE". A presente invenção se refere a genes modificados de neomicina fosfotranferase e ao uso dos mesmos em um método de seleção de células recombinantes com elevada reprodução. A invenção se refere ainda a vetores de expressão contendo gene modificado de neomicina fosfotransferase e um gene de interesse em uma ligação funcional com um promotor heterólogo, e a um método para produção de produtos de gene heterólogo pelo uso dos ditos vetores de expressão.
BR0316525-6A 2002-11-29 2003-11-25 Genes de fosfotransferase de neomicina e processos para seleção de células recombinantes de alta produtividade BR0316525A (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DE10256081 2002-11-29
DE10330686A DE10330686A1 (de) 2002-11-29 2003-07-08 Neue Neomycin-Phosphotransferase-Gene und Verfahren zur Selektion von Hochproduzierenden rekombinanten Zellen
PCT/EP2003/013203 WO2004050884A2 (de) 2002-11-29 2003-11-25 Neue neomycin-phosphotransferase-gene und verfahren zur selektion von hochproduzierenden rekombinanten zellen

Publications (1)

Publication Number Publication Date
BR0316525A true BR0316525A (pt) 2005-10-04

Family

ID=32471486

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0316525-6A BR0316525A (pt) 2002-11-29 2003-11-25 Genes de fosfotransferase de neomicina e processos para seleção de células recombinantes de alta produtividade

Country Status (12)

Country Link
EP (1) EP1567652B1 (pt)
JP (1) JP4344325B2 (pt)
KR (1) KR101149777B1 (pt)
AT (1) ATE371035T1 (pt)
AU (1) AU2003302689B2 (pt)
BR (1) BR0316525A (pt)
CA (1) CA2507664C (pt)
DE (1) DE50308030D1 (pt)
DK (1) DK1567652T3 (pt)
ES (1) ES2293096T3 (pt)
MX (1) MXPA05005481A (pt)
WO (1) WO2004050884A2 (pt)

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Also Published As

Publication number Publication date
JP2006507842A (ja) 2006-03-09
ES2293096T3 (es) 2008-03-16
DK1567652T3 (da) 2007-11-26
WO2004050884A2 (de) 2004-06-17
EP1567652A2 (de) 2005-08-31
CA2507664A1 (en) 2004-06-17
DE50308030D1 (de) 2007-10-04
JP4344325B2 (ja) 2009-10-14
KR101149777B1 (ko) 2012-06-11
ATE371035T1 (de) 2007-09-15
WO2004050884A3 (de) 2004-07-15
EP1567652B1 (de) 2007-08-22
CA2507664C (en) 2010-09-07
AU2003302689A1 (en) 2004-06-23
MXPA05005481A (es) 2005-07-25
KR20050085202A (ko) 2005-08-29
AU2003302689B2 (en) 2009-02-05

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B15K Others concerning applications: alteration of classification

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Ipc: C12N 15/85 (2006.01), C12N 9/12 (2006.01), C12N 5/

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2343 DE 01-12-2015 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.