BR112022014367A2 - Produção de hmo - Google Patents

Produção de hmo

Info

Publication number
BR112022014367A2
BR112022014367A2 BR112022014367A BR112022014367A BR112022014367A2 BR 112022014367 A2 BR112022014367 A2 BR 112022014367A2 BR 112022014367 A BR112022014367 A BR 112022014367A BR 112022014367 A BR112022014367 A BR 112022014367A BR 112022014367 A2 BR112022014367 A2 BR 112022014367A2
Authority
BR
Brazil
Prior art keywords
hmo
production
relates
recombinant production
hmo production
Prior art date
Application number
BR112022014367A
Other languages
English (en)
Inventor
Pedersen Margit
Bych Kampmann Katrine
Original Assignee
Glycom As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Glycom As filed Critical Glycom As
Publication of BR112022014367A2 publication Critical patent/BR112022014367A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • C07K14/24—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria from Enterobacteriaceae (F), e.g. Citrobacter, Serratia, Proteus, Providencia, Morganella, Yersinia
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/04—Polysaccharides, i.e. compounds containing more than five saccharide radicals attached to each other by glycosidic bonds
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Microbiology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PRODUÇÃO DE HMO. A presente invenção refere-se ao campo da produção recombinante de moléculas biológicas em células hospedeiras. Mais particularmente, refere-se a um método para a produção recombinante de oligossacarídeos do leite humano (HMO) usando uma célula geneticamente modificada que expressa uma proteína da superfamília de facilitadores principais (MFS).
BR112022014367A 2020-01-23 2021-01-22 Produção de hmo BR112022014367A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
DKPA202000086 2020-01-23
PCT/EP2021/051472 WO2021148614A1 (en) 2020-01-23 2021-01-22 Hmo production

Publications (1)

Publication Number Publication Date
BR112022014367A2 true BR112022014367A2 (pt) 2022-09-13

Family

ID=74556857

Family Applications (1)

Application Number Title Priority Date Filing Date
BR112022014367A BR112022014367A2 (pt) 2020-01-23 2021-01-22 Produção de hmo

Country Status (7)

Country Link
US (1) US12428657B2 (pt)
EP (1) EP4093747A1 (pt)
JP (1) JP2023511522A (pt)
CN (1) CN115003687B (pt)
AU (1) AU2021210607A1 (pt)
BR (1) BR112022014367A2 (pt)
WO (1) WO2021148614A1 (pt)

Families Citing this family (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK180952B1 (en) * 2020-12-22 2022-08-10 Glycom As A dfl-producing strain
CN116745430A (zh) * 2021-01-20 2023-09-12 因比奥斯公司 在宿主细胞中产生包含ln3作为核心结构的寡糖
BR112023014557A2 (pt) 2021-01-22 2023-10-17 Glycom As Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado
DK181991B1 (en) 2021-11-11 2025-05-15 Dsm Ip Assets Bv Combined fermentation process for producing one or more human milk oligosaccharide(s) (hmo(s)) in escherichia coli
US20250290078A1 (en) 2022-04-29 2025-09-18 Dsm Ip Assets B.V. Hmo producing microorganism with increased robustness towards glucose gradients
DK182075B1 (en) * 2022-06-13 2025-07-02 Dsm Ip Assets Bv Genetically engineered cells comprising sigma factor modifications for production of heterologous products and methods for use of same
DK181765B1 (en) * 2022-07-15 2024-12-04 Dsm Ip Assets Bv Cells expressing new fucosyltransferases for in vivo synthesis of lnfp-iii, and methods and uses of same
EP4577061A1 (en) 2022-08-25 2025-07-02 DSM IP Assets B.V. Hybrid method for producing complex hmos
WO2024110667A1 (en) 2022-11-25 2024-05-30 Dsm Ip Assets B.V. Two-strain system for producing oligosaccharides
CN117867054B (zh) * 2023-01-09 2024-09-27 山东恒鲁生物科技有限公司 合成hmo的方法
WO2024168219A2 (en) * 2023-02-09 2024-08-15 Amyris, Inc. Compositions and methods for improved production of human milk oligosaccharides
CN120677242A (zh) 2023-02-24 2025-09-19 帝斯曼知识产权资产管理有限公司 用于体内合成人乳寡糖的产物特异性转运蛋白
DK181822B1 (en) * 2023-06-07 2025-01-30 Dsm Ip Assets Bv Genetically engineered strains with reduced byproduct formation and methods and uses of same
DK182227B1 (en) 2023-10-17 2025-12-18 Dsm Ip Assets Bv Genetically engineered cells comprising new fucosyltransferases for in vivo synthesis of complex fucosylated human milk oligosaccharides mixtures comprising lnfp-vi, methods using the same, and uses of new fucosyltransferases thereof.
DK202430472A1 (en) 2024-08-19 2026-02-24 Dsm Ip Assets Bv Alpha-1,2 fucosyltransferase mutants with increased alpha 1,3-fucosyltransferase activity

Family Cites Families (14)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2440661T3 (en) * 2009-06-08 2018-03-12 Jennewein Biotechnologie Gmbh HMO synthesis
EP2675899A4 (en) 2011-02-16 2014-09-10 Glycosyn LLC BIOSYNTHESIS OF HUMAN MILK OLIGOSACCHARIDES IN MANIPULATED BACTERIA
EP3461890B1 (en) * 2014-03-31 2025-09-24 Chr. Hansen HMO GmbH Total fermentation of oligosaccharides
DE202015009782U1 (de) 2014-06-11 2020-02-10 Glycom A/S Trennung von 2'-O-Fucosyllactose aus Fermentationsbrühe
EP3191499A4 (en) 2014-09-09 2018-06-06 Glycosyn LLC Alpha (1,3) fucosyltransferases for use in the production of fucosylated oligosaccharides
US10676770B2 (en) 2014-12-16 2020-06-09 Glycom A/S Separation of 2′-FL from a fermentation broth
EP3141610A1 (en) 2015-09-12 2017-03-15 Jennewein Biotechnologie GmbH Production of human milk oligosaccharides in microbial hosts with engineered import / export
DE202017007249U1 (de) 2016-03-07 2020-04-23 Glycom A/S Abtrennung von Oligosacchariden aus der Fermentationsbrühe
EP4674526A3 (en) 2016-04-19 2026-04-01 Glycom A/S Separation of oligosaccharides from fermentation broth
ES2856749T3 (es) * 2016-10-29 2021-09-28 Chr Hansen Hmo Gmbh Proceso para la producción de oligosacáridos fucosilados
US11608504B2 (en) 2017-12-21 2023-03-21 Glycom A/S Nucleic acid construct for in vitro and in vivo gene expression
US20190323052A1 (en) * 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing export of 2? fucosyllactose from microbial cells through the expression of a heterologous nucleic acid
US20190323053A1 (en) 2018-04-23 2019-10-24 Dupont Nutrition Biosciences Aps Increasing activity of 2? fucosyllactose transporters endogenous to microbial cells
EP3569713A1 (en) * 2018-05-16 2019-11-20 Jennewein Biotechnologie GmbH Use of glycosidases in the production of oligosaccharides

Also Published As

Publication number Publication date
JP2023511522A (ja) 2023-03-20
CN115003687A (zh) 2022-09-02
WO2021148614A1 (en) 2021-07-29
AU2021210607A1 (en) 2022-07-14
US12428657B2 (en) 2025-09-30
US20230227876A1 (en) 2023-07-20
CN115003687B (zh) 2024-12-06
EP4093747A1 (en) 2022-11-30

Similar Documents

Publication Publication Date Title
BR112022014367A2 (pt) Produção de hmo
BR112022014423A2 (pt) Produção de hmo
BR112023014557A2 (pt) Nova proteína (fred) da superfamília de facilitadores principais (mfs) na produção de hmos sialiado
BR112022014414A2 (pt) Produção de hmo
Nwachukwu et al. Thermoase-derived flaxseed protein hydrolysates and membrane ultrafiltration peptide fractions have systolic blood pressure-lowering effects in spontaneously hypertensive rats
Sousa et al. Valorization of by-products from commercial fish species: Extraction and chemical properties of skin gelatins
Hsu et al. Maternal N-acetylcysteine therapy prevents hypertension in spontaneously hypertensive rat offspring: implications of hydrogen sulfide-generating pathway and gut microbiota
Valcarcel et al. Production and physicochemical characterization of gelatin and collagen hydrolysates from turbot skin waste generated by aquaculture activities
Qian et al. Effects of taurine on gut microbiota homeostasis: an evaluation based on two models of gut dysbiosis
Mahbub et al. Plasma branched-chain and aromatic amino acids in relation to hypertension
Lee et al. Analysis of skin regeneration and barrier-improvement efficacy of polydeoxyribonucleotide isolated from Panax ginseng (CA Mey.) adventitious root
BR112022011904A2 (pt) Produção de oligossacarídeo sialilado em células hospedeiras
Hsu et al. Protection of male rat offspring against hypertension programmed by prenatal dexamethasone administration and postnatal high-fat diet with the Nrf2 activator dimethyl fumarate during pregnancy
BRPI1006077B8 (pt) Sistema para analisar uma ou mais propriedades de carne
BR112014023869A2 (pt) proteínas nutritivas carregadas e métodos
BRPI0514870A (pt) sistemas e métodos para a produção de proteìna
Stellavato et al. Chondroitin sulfate in USA dietary supplements in comparison to pharma grade products: analytical fingerprint and potential anti-inflammatory effect on human osteoartritic chondrocytes and synoviocytes
BR112022002584A2 (pt) Célula de levedura, método para produzir um ou mais oligossacarídeos, composição de fermentação, método para recuperar um ou mais oligossacarídeos, método para modificar geneticamente uma célula de levedura
Ma et al. Integrative analysis of proteomics and transcriptomics of longissimus dorsi with different feeding systems in yaks
BR112023004871A2 (pt) Métodos para reduzir o conteúdo de proteínas das células hospedeiras em processos de purificação de proteínas
Mahtout et al. Algerian carob tree products: A comprehensive valorization analysis and future prospects
Ali et al. Cryopreservation induces acetylation of metabolism-related proteins in boar sperm
Martínez-García et al. Autophagy deficiency by Atg4B loss leads to metabolomic alterations in mice
Paz et al. Premature vascular aging in guinea pigs affected by fetal growth restriction
Vassallo et al. Molecular fingerprint of human pathological synoviocytes in response to extractive sulfated and biofermentative unsulfated chondroitins

Legal Events

Date Code Title Description
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B11Y Definitive dismissal - extension of time limit for request of examination expired [chapter 11.1.1 patent gazette]