BRPI0514870A - sistemas e métodos para a produção de proteìna - Google Patents

sistemas e métodos para a produção de proteìna

Info

Publication number
BRPI0514870A
BRPI0514870A BRPI0514870-7A BRPI0514870A BRPI0514870A BR PI0514870 A BRPI0514870 A BR PI0514870A BR PI0514870 A BRPI0514870 A BR PI0514870A BR PI0514870 A BRPI0514870 A BR PI0514870A
Authority
BR
Brazil
Prior art keywords
protein
genetically engineered
methods
interest
cells
Prior art date
Application number
BRPI0514870-7A
Other languages
English (en)
Inventor
Yijie Gao
Mei Geng
Stephen H Herrmann
Xiaotian Zhong
Ronald Kriz
Zhijian Lu
Nicole M Piche
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of BRPI0514870A publication Critical patent/BRPI0514870A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/46—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates
    • C07K14/47—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals
    • C07K14/4701—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans from vertebrates from mammals not used
    • C07K14/4702—Regulators; Modulating activity
    • C07K14/4705—Regulators; Modulating activity stimulating, promoting or activating activity
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/51—Bone morphogenetic factor; Osteogenins; Osteogenic factor; Bone-inducing factor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • A—HUMAN NECESSITIES
    • A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01K—ANIMAL HUSBANDRY; AVICULTURE; APICULTURE; PISCICULTURE; FISHING; REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR; NEW BREEDS OF ANIMALS
    • A01K2217/00—Genetically modified animals
    • A01K2217/07—Animals genetically altered by homologous recombination
    • A01K2217/075—Animals genetically altered by homologous recombination inducing loss of function, i.e. knock out

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • General Engineering & Computer Science (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Biomedical Technology (AREA)
  • Toxicology (AREA)
  • Microbiology (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Medicines That Contain Protein Lipid Enzymes And Other Medicines (AREA)

Abstract

SISTEMAS E MéTODOS PARA A PRODUçãO DE PROTEìNA A presente invenção refere-se a sistemas e métodos para a produção de proteínas de interesse. A invenção emprega células animais ou vegetais geneticamente manipuladas, que tenham capacidades modificadas de dobramento ou processamento de proteínas. Em um aspecto, a invenção apresenta células geneticamente manipuladas compreendendo um ou mais cassetes de expressão recombinantes que codificam (1) uma proteína de interesse e (2) um polipeptídio que seja funcional na via de resposta de proteína não dobrada (UPR) das células. A co-expressão do polipeptídio aumenta significativamente o rendimento da proteína de interesse nas células geneticamente manipuladas. Em um exemplo, as células geneticamente manipuladas são células animais, e o polipeptídio co-expressado é um componente ou modulador de uma via UPR mediada por XBP1 ou ATF6.
BRPI0514870-7A 2004-09-02 2005-09-01 sistemas e métodos para a produção de proteìna BRPI0514870A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US60643904P 2004-09-02 2004-09-02
PCT/US2005/031081 WO2006028889A2 (en) 2004-09-02 2005-09-01 Systems and methods for protein production

Publications (1)

Publication Number Publication Date
BRPI0514870A true BRPI0514870A (pt) 2008-06-24

Family

ID=36036848

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514870-7A BRPI0514870A (pt) 2004-09-02 2005-09-01 sistemas e métodos para a produção de proteìna

Country Status (16)

Country Link
US (3) US7514237B2 (pt)
EP (2) EP1784490B1 (pt)
JP (2) JP2008511335A (pt)
KR (1) KR20070053732A (pt)
CN (1) CN101048509A (pt)
AU (1) AU2005282746A1 (pt)
BR (1) BRPI0514870A (pt)
CA (1) CA2577073C (pt)
CR (1) CR8927A (pt)
EC (1) ECSP077295A (pt)
ES (2) ES2653843T3 (pt)
IL (1) IL181278A0 (pt)
MX (1) MX2007002379A (pt)
NO (1) NO20071221L (pt)
RU (1) RU2007106442A (pt)
WO (1) WO2006028889A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5548932B2 (ja) * 2006-09-25 2014-07-16 国立大学法人大阪大学 Ａｔｆ４および／またはｇａｄｄ３４を利用した蛋白質高生産方法
CA2726289A1 (en) * 2008-05-28 2009-12-23 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in hbx-expressing mammalian cells
WO2009149021A2 (en) 2008-06-02 2009-12-10 Dana-Farber Cancer Institute, Inc. Xbp1, cd138, and cs1 peptides
US9315565B2 (en) 2009-02-10 2016-04-19 Ucb Pharma, S.A. Method for producing protein
GB0902180D0 (en) * 2009-02-10 2009-03-25 Ucb Pharma Sa Method for producing protein
WO2010151827A1 (en) * 2009-06-25 2010-12-29 Bayer Healthcare Llc X-box binding proteins (xbp-1) variants and methods of using the same
TW201823460A (zh) 2012-05-29 2018-07-01 美商再生元醫藥公司 生產細胞株增強子
CL2015003242A1 (es) * 2015-11-04 2016-10-14 Univ Chile Virus aav/upr-plus, proteína de fusión upr-plus, método de tratamiento genético y su uso en el tratamiento enfermedades neurodegenerativas, tales como las enfermedades de parkinson y huntington, entre otras.
WO2019010551A1 (pt) * 2017-07-10 2019-01-17 Instituto De Biologia Molecular Do Paraná - Ibmp Plataforma genética para superexpressão heteróloga associada à seleção de células altamente produtoras de proteínas
CN107858378B (zh) * 2017-11-20 2020-11-10 中国科学院生物物理研究所 衰老细胞模型及其制备方法
CN110093361B (zh) * 2019-04-17 2023-05-09 拜澳泰克(沈阳)生物医学集团有限公司 一种增强基因表达的增强子多肽及其应用
WO2022067214A2 (en) 2020-09-28 2022-03-31 Vestaron Corporation Mu-diguetoxin-dc1a variant polypeptides for pest control
WO2022136140A1 (en) * 2020-12-22 2022-06-30 F. Hoffmann-La Roche Ag Oligonucleotides targeting xbp1
WO2022235929A1 (en) 2021-05-05 2022-11-10 Radius Pharmaceuticals, Inc. Animal model having homologous recombination of mouse pth1 receptor
WO2023122805A1 (en) 2021-12-20 2023-06-29 Vestaron Corporation Sorbitol driven selection pressure method
CA3256326A1 (en) 2022-06-09 2023-12-14 CHO Plus Inc. CELL HYBRIDS AS HOST CELLS FOR HIGH-YIELD PRODUCTION OF GENE THERAPY VECTORS AND VIRAL VACCINES
WO2024026406A2 (en) 2022-07-29 2024-02-01 Vestaron Corporation Next Generation ACTX Peptides
WO2025024776A2 (en) * 2023-07-27 2025-01-30 Duke University Compositions comprising xbp1 fragments and methods of use in gene therapy
WO2026015884A1 (en) * 2024-07-11 2026-01-15 Helix Nanotechnologies Inc Compositions comprising ire-1 binding sequences and uses thereof
CN120888609B (zh) * 2025-10-10 2025-12-23 汇智和源生物技术(苏州)有限公司 一种瞬时高效表达人源slc转运体oatp1b3的方法

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US6063630A (en) 1991-11-05 2000-05-16 Transkaryotic Therapies, Inc. Targeted introduction of DNA into primary or secondary cells and their use for gene therapy
ES2256972T3 (es) 1997-11-18 2006-07-16 Pioneer Hi-Bred International, Inc. Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas.
WO2000001835A2 (en) 1998-07-01 2000-01-13 Austrian Nordic Biotherapeutics Ag Targeted integration into chromosomes using retroviral vectors
JP2001054391A (ja) * 1998-11-13 2001-02-27 Hsp Kenkyusho:Kk 小胞体ストレス転写因子
CA2403842C (en) 2000-03-24 2012-09-25 Genencor International, Inc. Increased production of secreted proteins by recombinant eukaryotic cells
WO2003089622A2 (en) * 2002-04-22 2003-10-30 University Of Michigan Novel genes, compositions, and methods for modulating the unfolded protein response
JP4446057B2 (ja) * 2002-08-28 2010-04-07 独立行政法人理化学研究所 生体刺激存在下でのｍＲＮＡのフレームシフトを利用した蛋白質の発現方法
US20050106222A1 (en) * 2003-06-11 2005-05-19 Ailor Eric N. Method to increase protein production in culture

Also Published As

Publication number Publication date
US20120322104A1 (en) 2012-12-20
KR20070053732A (ko) 2007-05-25
NO20071221L (no) 2007-03-30
CR8927A (es) 2007-08-28
AU2005282746A1 (en) 2006-03-16
US7514237B2 (en) 2009-04-07
RU2007106442A (ru) 2008-10-10
US20090221035A1 (en) 2009-09-03
EP1784490A2 (en) 2007-05-16
US8173394B2 (en) 2012-05-08
ES2649862T3 (es) 2018-01-16
CA2577073A1 (en) 2006-03-16
ES2653843T3 (es) 2018-02-09
IL181278A0 (en) 2007-07-04
EP1784490B1 (en) 2017-11-01
JP5508460B2 (ja) 2014-05-28
EP2316955A1 (en) 2011-05-04
US20060053502A1 (en) 2006-03-09
EP2316955B1 (en) 2017-10-04
MX2007002379A (es) 2007-04-23
WO2006028889A3 (en) 2006-10-05
CA2577073C (en) 2016-10-11
ECSP077295A (es) 2007-04-26
JP2008511335A (ja) 2008-04-17
CN101048509A (zh) 2007-10-03
WO2006028889A2 (en) 2006-03-16
JP2012161318A (ja) 2012-08-30

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Legal Events

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B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 7A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2166 DE 10/07/2012.