BR9710815A - N7- metiltransferase de xantosina substancialmente pura, sequência de ácido nucleico substancialmente pura, e processo para produzir grãos de café sem cafeìna. - Google Patents

N7- metiltransferase de xantosina substancialmente pura, sequência de ácido nucleico substancialmente pura, e processo para produzir grãos de café sem cafeìna.

Info

Publication number
BR9710815A
BR9710815A BR9710815-4A BR9710815A BR9710815A BR 9710815 A BR9710815 A BR 9710815A BR 9710815 A BR9710815 A BR 9710815A BR 9710815 A BR9710815 A BR 9710815A
Authority
BR
Brazil
Prior art keywords
substantially pure
caffeine
nucleic acid
acid sequence
expression
Prior art date
Application number
BR9710815-4A
Other languages
English (en)
Inventor
John I Stiles
Istefo Moisyadi
Kabi Raj Neupane
Original Assignee
Univ Hawaii
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Hawaii filed Critical Univ Hawaii
Publication of BR9710815A publication Critical patent/BR9710815A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/1003—Transferases (2.) transferring one-carbon groups (2.1)
    • C12N9/1007—Methyltransferases (general) (2.1.1.)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Tea And Coffee (AREA)
  • Peptides Or Proteins (AREA)

Abstract

"N^ 7^ -METILTRANSFERASE DE XANTOSINA SUBSTANCIALMENTE PURA, SEQUêNCIA DE áCIDO NUCLEICO SUBSTANCIALMENTE PURA, E, PROCESSO PARA PRODUZIR GRãOS DE CAFé SEM CAFEìNA". Proteínas purificadas, seq³ências de DNA que codificam em expressão assim e moléculas de DNA recombinantes, incluindo hospedeiros transformados com as mesmas, para transformar plantas de café para suprimir a expressão de caféina. As sequências de DNA e moléculas de DNA recombinantes são caracterizadas em que elas codificam em expressão para uma enzima na via para síntese de cafeína em café. As plantas de café transformadas com moléculas de DNA que codificam em transcrição para mRNA que é anti-sentido para mRNA, que codifica em expressão durante pelo menos uma enzima na via para bio-síntese de cafeína.
BR9710815-4A 1996-03-26 1997-03-24 N7- metiltransferase de xantosina substancialmente pura, sequência de ácido nucleico substancialmente pura, e processo para produzir grãos de café sem cafeìna. BR9710815A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US08/622,679 US6075184A (en) 1996-03-26 1996-03-26 Purified proteins, recombinant DNA sequences and processes for producing caffeine free beverages
PCT/US1997/004982 WO1997035960A1 (en) 1996-03-26 1997-03-24 Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Publications (1)

Publication Number Publication Date
BR9710815A true BR9710815A (pt) 2000-01-11

Family

ID=24495092

Family Applications (1)

Application Number Title Priority Date Filing Date
BR9710815-4A BR9710815A (pt) 1996-03-26 1997-03-24 N7- metiltransferase de xantosina substancialmente pura, sequência de ácido nucleico substancialmente pura, e processo para produzir grãos de café sem cafeìna.

Country Status (23)

Country Link
US (2) US6075184A (pt)
EP (1) EP0862635B1 (pt)
JP (1) JP2001519649A (pt)
KR (1) KR100561889B1 (pt)
AP (1) AP752A (pt)
AT (1) ATE342363T1 (pt)
AU (1) AU711481B2 (pt)
BR (1) BR9710815A (pt)
CA (1) CA2257120A1 (pt)
CZ (1) CZ295397B6 (pt)
DE (1) DE69736801T2 (pt)
EA (1) EA003835B1 (pt)
ES (1) ES2275285T3 (pt)
FI (1) FI982070L (pt)
HU (1) HUP0300851A3 (pt)
IS (1) IS4851A (pt)
NO (1) NO324871B1 (pt)
NZ (1) NZ330290A (pt)
OA (1) OA11175A (pt)
PT (1) PT862635E (pt)
TR (1) TR199801909T2 (pt)
UA (1) UA73465C2 (pt)
WO (1) WO1997035960A1 (pt)

Families Citing this family (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6448474B1 (en) 1995-06-07 2002-09-10 University Of Hawaii Purified proteins, recombinant DNA sequences and processes for controlling the ripening of coffee plants
WO1998042848A1 (en) * 1997-03-24 1998-10-01 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
AU6324798A (en) * 1998-02-13 1998-09-08 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages
US6930227B1 (en) * 1999-05-26 2005-08-16 Mitsui Chemicals, Inc. Camellia sinensis gene encoding a caffeine synthesis associated n-methyl transferase with 7-methylxanthine n3 methyl transferase, theobromine n1 methyl transferase, and paraxanthine n3 methyl transferase activities and use thereof
JP3520328B2 (ja) 2000-10-06 2004-04-19 奈良先端科学技術大学院大学長 コーヒー属植物のテオブロミン合成酵素ポリペプチド及び当該ポリペプチドをコードする遺伝子
JP2004049022A (ja) * 2002-07-16 2004-02-19 Nara Institute Of Science & Technology 遺伝子組換えによるカフェインレスコーヒー植物の製造方法
US8609366B2 (en) * 2006-07-28 2013-12-17 Legacy Emanuel Hospital & Health Center Method and systems for tissue culture
GB0803068D0 (en) * 2008-02-20 2008-03-26 Health Prot Agency Cross-linked biological indicator
US10466245B2 (en) 2008-02-20 2019-11-05 The Secretary Of State For Health Covalently linked thermostable kinase for decontamination process validation
CN105861408B (zh) * 2016-06-22 2019-11-19 安徽农业大学 发酵生产咖啡碱的工程菌、其构建方法及应用
US20200270604A1 (en) 2017-09-19 2020-08-27 Tropic Biosciences UK Limited Modifying the specificity of non-coding rna molecules for silencing gene expression in eukaryotic cells
GB201807192D0 (en) * 2018-05-01 2018-06-13 Tropic Biosciences Uk Ltd Compositions and methods for reducing caffeine content in coffee beans

Family Cites Families (13)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5453566A (en) 1986-03-28 1995-09-26 Calgene, Inc. Antisense regulation of gene expression in plant/cells
IL81737A (en) * 1986-03-28 1992-11-15 Calgene Inc Regulation of gene expression in plant cells
US5296376A (en) 1986-11-04 1994-03-22 Imperial Chemical Industries Plc DNA, constructs, cells and plants derived therefrom
US5436395A (en) 1988-11-07 1995-07-25 Kraft Foods, Inc. Induction and selection of somaclonal variation in coffee
EP0463056A1 (en) 1989-03-17 1992-01-02 E.I. Du Pont De Nemours And Company External regulation of gene expression
US5334529A (en) * 1989-06-27 1994-08-02 Escagenetics Corporation Stably transformed coffee plant cells and plantlets
GB8916213D0 (en) * 1989-07-14 1989-08-31 Ici Plc Dna constructs,cells and plants derived therefrom
MX9100993A (es) * 1990-09-10 1992-05-04 Us Agriculture Secuencia de adn aislado y enzima acido 1-aminociclopropan-1-carboxilico sintasa,recombinante
US5457041A (en) 1994-03-25 1995-10-10 Science Applications International Corporation Needle array and method of introducing biological substances into living cells using the needle array
AU715924B2 (en) * 1994-09-02 2000-02-10 Seminis Vegetable Seeds, Inc. Transgenic plants expressing ACC oxidase genes
US5633440A (en) * 1994-12-20 1997-05-27 Dna Plant Technology Corporation P119 promoters and their uses
WO1996021027A1 (en) * 1994-12-30 1996-07-11 Asgrow Seed Company Transgenic plants expressing acc synthase gene
AU6324798A (en) * 1998-02-13 1998-09-08 University Of Hawaii Purified proteins, recombinant dna sequences and processes for producing caffeine free beverages

Also Published As

Publication number Publication date
CZ309198A3 (cs) 1999-08-11
EA199800765A1 (ru) 1999-10-28
UA73465C2 (en) 2005-08-15
CA2257120A1 (en) 1997-10-02
NO984463D0 (no) 1998-09-25
HUP0300851A3 (en) 2007-05-02
FI982070A7 (fi) 1998-10-26
US6075184A (en) 2000-06-13
FI982070L (fi) 1998-10-26
WO1997035960A8 (en) 2000-04-06
NO324871B1 (no) 2007-12-17
OA11175A (en) 2003-05-14
EP0862635A4 (pt) 1998-09-16
WO1997035960A1 (en) 1997-10-02
KR100561889B1 (ko) 2006-07-06
EP0862635A1 (en) 1998-09-09
DE69736801T2 (de) 2007-08-16
US6348641B1 (en) 2002-02-19
IS4851A (is) 1998-09-23
DE69736801D1 (de) 2006-11-23
AP9801347A0 (en) 1998-09-30
ATE342363T1 (de) 2006-11-15
ES2275285T3 (es) 2007-06-01
CZ295397B6 (cs) 2005-08-17
EP0862635B1 (en) 2006-10-11
EA003835B1 (ru) 2003-10-30
KR20040069364A (ko) 2004-08-06
TR199801909T2 (xx) 2000-06-21
JP2001519649A (ja) 2001-10-23
PT862635E (pt) 2007-01-31
HUP0300851A2 (hu) 2003-08-28
FI982070A0 (fi) 1998-09-25
NO984463L (no) 1998-11-24
NZ330290A (en) 1999-11-29
AU4511197A (en) 1998-04-24
AU711481B2 (en) 1999-10-14
AP752A (en) 1999-07-12

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements