BR9712942A - Método para a produção de glicerol, célula hospedeira transformada, método para a seleção da expressão do gene, cepa pah21/dh5x de escherichia coli e cepa pah21/dh5x de escherichia coli. - Google Patents

Método para a produção de glicerol, célula hospedeira transformada, método para a seleção da expressão do gene, cepa pah21/dh5x de escherichia coli e cepa pah21/dh5x de escherichia coli.

Info

Publication number
BR9712942A
BR9712942A BR9712942-9A BR9712942A BR9712942A BR 9712942 A BR9712942 A BR 9712942A BR 9712942 A BR9712942 A BR 9712942A BR 9712942 A BR9712942 A BR 9712942A
Authority
BR
Brazil
Prior art keywords
pah21
dh5x
strain
escherichia coli
glycerol
Prior art date
Application number
BR9712942-9A
Other languages
English (en)
Other versions
BR9712942B1 (pt
Inventor
Ben A Bulthuis
Anthony Arthur Gatenby
Sharon Loretta Haynie
Amy Kuang-Hua Hsu
Original Assignee
Du Pont
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Du Pont, Genencor Int filed Critical Du Pont
Publication of BR9712942A publication Critical patent/BR9712942A/pt
Publication of BR9712942B1 publication Critical patent/BR9712942B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/52—Genes encoding for enzymes or proenzymes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/18—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic polyhydric
    • C12P7/20—Glycerol
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression

Landscapes

  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Molecular Biology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Agricultural Chemicals And Associated Chemicals (AREA)

Abstract

<B>"MéTODO PARA A PRODUçãO DE GLICEROL, CéLULA HOSPEDEIRA TRANSFORMADA, MéTODO PARA A SELEçãO DA EXPRESSãO DO GENE, CEPA pAH21/DH5<244> DE Escherichia coli E CEPA pAH21/DH5<244> DE Escherichia coli"<D>. A presente invenção trata de organismos recombinantes que compreendem genes que codificam uma atividade de glocerol-3-fosfato desidrogenase e/ou de glicerol-3-fosfatase útil para a produção de glicerol a partir de uma variedade de substratos de carbono.
BRPI9712942-9A 1996-11-13 1997-11-10 método para a produção de glicerol e célula hospedeira de e. coli transformada. BR9712942B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US3060296P 1996-11-13 1996-11-13
PCT/US1997/020293 WO1998021340A1 (en) 1996-11-13 1997-11-10 Method for the production of glycerol by recombinant organisms

Publications (2)

Publication Number Publication Date
BR9712942A true BR9712942A (pt) 2000-04-04
BR9712942B1 BR9712942B1 (pt) 2010-09-21

Family

ID=21855002

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI9712942-9A BR9712942B1 (pt) 1996-11-13 1997-11-10 método para a produção de glicerol e célula hospedeira de e. coli transformada.

Country Status (16)

Country Link
US (2) US6358716B1 (pt)
EP (1) EP0948628B1 (pt)
JP (1) JP4330041B2 (pt)
KR (1) KR100674074B1 (pt)
CN (1) CN1174100C (pt)
AT (1) ATE471984T1 (pt)
AU (1) AU5430798A (pt)
BR (1) BR9712942B1 (pt)
CA (2) CA2625060A1 (pt)
DE (1) DE69739918D1 (pt)
ES (1) ES2345469T3 (pt)
ID (1) ID21555A (pt)
IL (2) IL129723A0 (pt)
MY (1) MY130130A (pt)
WO (1) WO1998021340A1 (pt)
ZA (1) ZA9710199B (pt)

Families Citing this family (33)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN1202253C (zh) 1997-12-02 2005-05-18 纳幕尔杜邦公司 用重组生物体生产甘油的方法
US6432686B1 (en) 1998-05-12 2002-08-13 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant organisms comprising genes for vitamin B12 transport
US7074608B1 (en) 1998-05-12 2006-07-11 E. I. Du Pont De Nemours And Company Method for the production of 1,3-propanediol by recombinant organisms comprising genes for coenzyme B12 synthesis
US6365410B1 (en) 1999-05-19 2002-04-02 Genencor International, Inc. Directed evolution of microorganisms
US6852517B1 (en) 1999-08-30 2005-02-08 Wisconsin Alumni Research Foundation Production of 3-hydroxypropionic acid in recombinant organisms
US7759547B2 (en) * 1999-09-22 2010-07-20 National Research Council Of Canada Methods of producing and growing plants having improved phosphorus utilization
US6803218B1 (en) 1999-09-24 2004-10-12 Genencor Intl., Inc. Enzymes which dehydrate glycerol
US7022674B2 (en) 1999-12-16 2006-04-04 Eli Lilly And Company Polypeptide compositions with improved stability
CN1298855C (zh) 2001-04-04 2007-02-07 金克克国际有限公司 在宿主细胞中生成产物的方法
ATE421577T1 (de) 2001-04-04 2009-02-15 Genencor Int Entkoppelte anabole und katabole stoffwechselwege in wirtszellen
DE602004028359D1 (de) 2003-05-06 2010-09-09 Du Pont Reinigung von biologisch produziertem 1,3-propandiol
KR20040104165A (ko) * 2003-06-03 2004-12-10 씨제이 주식회사 캔디다 알비칸스 유래의 글리세롤-3-포스페이트포스파타제 및 글리세롤-3-포스페이트 디히드로게나제,이를 코딩하는 유전자, 상기 유전자를 포함하는 벡터 및숙주 세포, 및 상기 숙주세포를 이용한 글리세롤의 생산방법
US20070029252A1 (en) * 2005-04-12 2007-02-08 Dunson James B Jr System and process for biomass treatment
US7781191B2 (en) * 2005-04-12 2010-08-24 E. I. Du Pont De Nemours And Company Treatment of biomass to obtain a target chemical
JP4991700B2 (ja) * 2005-04-12 2012-08-01 イー・アイ・デュポン・ドウ・ヌムール・アンド・カンパニー バイオマス処理のためのシステムおよび方法
DE102005018835A1 (de) * 2005-04-22 2006-11-02 Degussa Ag Verfahren zur Herstellung von L-Aminosäuren unter Verwendung verbesserter Stämme der Familie Enterobacteriaceae
US7988883B2 (en) 2006-02-10 2011-08-02 Dupont Tate & Lyle Bio Products Company, Llc Heat transfer compositions comprising renewably-based biodegradable 1,3-propanediol
US20070275139A1 (en) * 2006-02-10 2007-11-29 Melissa Joerger Food compositions comprising renewably-based, biodegradable1,3-propanediol
WO2008061187A1 (en) * 2006-11-15 2008-05-22 Dupont Tate & Lyle Bio Products Company, Llc Preservative compositions comprising renewably-based, biodegradable 1,3-propanediol
US20090053800A1 (en) * 2007-08-22 2009-02-26 Julie Friend Biomass Treatment Apparatus
US8445236B2 (en) 2007-08-22 2013-05-21 Alliance For Sustainable Energy Llc Biomass pretreatment
EP2594646A3 (en) 2007-09-21 2013-11-06 BASF Plant Science GmbH Plant with increased yield
FR2927089B1 (fr) * 2008-02-05 2011-03-25 Inst Nat De La Rech Agronomique Inra Procede d'integration ciblee de multicopies d'un gene d'interet dans une souche de yarrowia
JP4963488B2 (ja) * 2008-04-23 2012-06-27 トヨタ自動車株式会社 変異体酵母及びこれを用いた物質生産方法
US20110136190A1 (en) * 2009-12-04 2011-06-09 E. I. Du Pont De Nemours And Company Recombinant bacteria for producing glycerol and glycerol-derived products from sucrose
US8129170B1 (en) * 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
WO2014062997A1 (en) * 2012-10-18 2014-04-24 Algenol Biofuels Inc. Production of 1, 3-propanediol in cyanobacteria
CN105189772B (zh) * 2013-03-15 2019-09-13 阿迈瑞斯公司 磷酸转酮酶和磷酸转乙酰酶生产乙酰-辅酶a衍生化合物的用途
KR20150110144A (ko) 2014-03-24 2015-10-02 삼성전자주식회사 글리세롤, 3-hp, 또는 아크릴산 생산능을 갖는 재조합 미생물 및 그를 이용하여 글리세롤, 3-hp, 또는 아크릴산을 생산하는 방법
US9968531B2 (en) 2015-08-05 2018-05-15 Dupont Tate & Lyle Bio Products Company, Llc Deodorants containing 1,3-propanediol
PL3559246T3 (pl) * 2016-12-20 2022-09-05 Dsm Ip Assets B.V. Ulepszone szczepy drożdży do wytwarzania etanolu
CN110205277A (zh) * 2019-05-14 2019-09-06 河北科技师范学院 肠炎沙门菌glpK基因缺失的应用
WO2024206168A1 (en) * 2023-03-24 2024-10-03 Natáur Llc Bioproduction of sulfur-containing compounds

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5599689A (en) 1995-05-12 1997-02-04 E. I. Du Pont De Nemours And Company Process for making 1,3-propanediol from carbohydrates using mixed microbial cultures
US5686276A (en) * 1995-05-12 1997-11-11 E. I. Du Pont De Nemours And Company Bioconversion of a fermentable carbon source to 1,3-propanediol by a single microorganism
US5633362A (en) * 1995-05-12 1997-05-27 E. I. Du Pont De Nemours And Company Production of 1,3-propanediol from glycerol by recombinant bacteria expressing recombinant diol dehydratase
FR2735145B1 (fr) * 1995-06-09 1997-08-22 Agronomique Inst Nat Rech Souches de levures presentant un bilan de fermentation alcoolique des sucres modifie et leurs applications, vecteurs utilisables pour l'obtention desdites souches.
US5733749A (en) 1995-08-14 1998-03-31 Wisconsin Alumni Research Foundation Glycerol phosphatase with stereo-specific activity
BR9712761B1 (pt) 1996-11-13 2010-02-23 mÉtodo para a produÇço de 1,3- propanodiol e microorganismo hospedeiro transformado.

Also Published As

Publication number Publication date
JP2001503634A (ja) 2001-03-21
CA2625060A1 (en) 1998-05-22
EP0948628A1 (en) 1999-10-13
IL129723A (en) 2008-07-08
ES2345469T3 (es) 2010-09-23
ATE471984T1 (de) 2010-07-15
KR100674074B1 (ko) 2007-01-26
JP4330041B2 (ja) 2009-09-09
WO1998021340A1 (en) 1998-05-22
ID21555A (id) 1999-06-24
CN1244216A (zh) 2000-02-09
CA2267198C (en) 2008-06-17
CA2267198A1 (en) 1998-05-22
BR9712942B1 (pt) 2010-09-21
IL129723A0 (en) 2000-02-29
ZA9710199B (en) 1999-05-12
MY130130A (en) 2007-06-29
DE69739918D1 (de) 2010-08-05
CN1174100C (zh) 2004-11-03
US20060177915A1 (en) 2006-08-10
EP0948628B1 (en) 2010-06-23
KR20000053212A (ko) 2000-08-25
US6358716B1 (en) 2002-03-19
AU5430798A (en) 1998-06-03

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Legal Events

Date Code Title Description
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 21/09/2010, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time
B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)