BRPI0406995A - Vetor de alta expressão para células animais - Google Patents

Vetor de alta expressão para células animais

Info

Publication number
BRPI0406995A
BRPI0406995A BR0406995-1A BRPI0406995A BRPI0406995A BR PI0406995 A BRPI0406995 A BR PI0406995A BR PI0406995 A BRPI0406995 A BR PI0406995A BR PI0406995 A BRPI0406995 A BR PI0406995A
Authority
BR
Brazil
Prior art keywords
expression vector
animal cells
high expression
animal
present
Prior art date
Application number
BR0406995-1A
Other languages
English (en)
Inventor
Masato Okabe
Tetsuo Nakamura
Original Assignee
Japan Immuno Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Japan Immuno Inc filed Critical Japan Immuno Inc
Publication of BRPI0406995A publication Critical patent/BRPI0406995A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/85—Vectors or expression systems specially adapted for eukaryotic hosts for animal cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/15—Vector systems having a special element relevant for transcription chimeric enhancer/promoter combination
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/42—Vector systems having a special element relevant for transcription being an intron or intervening sequence for splicing and/or stability of RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2830/00—Vector systems having a special element relevant for transcription
    • C12N2830/60—Vector systems having a special element relevant for transcription from viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/20—Vectors comprising a special translation-regulating system translation of more than one cistron
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2840/00—Vectors comprising a special translation-regulating system
    • C12N2840/44—Vectors comprising a special translation-regulating system being a specific part of the splice mechanism, e.g. donor, acceptor

Landscapes

  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

"VETOR DE ALTA EXPRESSãO PARA CéLULAS ANIMAIS". Um objetivo da presente invenção é proporcionar um vetor de expressão que permite a fácil obtenção de linhagens produtivas de alto nível, usando uma célula de mamífero e, especialmente, uma célula de ovário de hamster chinês (célula CHO) como hospedeira. A presente invenção proporciona um vetor de expressão que induz a alta produtividade de proteínas geneticamente recombinantes em células hospedeiras de animal, que consiste de um promotor altamente indutor de expressão, um sítio de multiclonagem para inserção de gene e uma seq³ência sinal de poliadenilação, nessa ordem de cima para baixo, e consiste ainda, a jusante, de um gene resistente a droga que não tem um promotor capaz de agir em células animais.
BR0406995-1A 2003-02-03 2004-02-03 Vetor de alta expressão para células animais BRPI0406995A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
JP2003025677 2003-02-03
PCT/JP2004/001066 WO2004070030A1 (ja) 2003-02-03 2004-02-03 動物細胞用高発現ベクター

Publications (1)

Publication Number Publication Date
BRPI0406995A true BRPI0406995A (pt) 2006-01-10

Family

ID=32844112

Family Applications (1)

Application Number Title Priority Date Filing Date
BR0406995-1A BRPI0406995A (pt) 2003-02-03 2004-02-03 Vetor de alta expressão para células animais

Country Status (10)

Country Link
EP (1) EP1591523A4 (pt)
JP (1) JPWO2004070030A1 (pt)
CN (1) CN1771324A (pt)
AR (1) AR043017A1 (pt)
BR (1) BRPI0406995A (pt)
CA (1) CA2514941A1 (pt)
MX (1) MXPA05008231A (pt)
TN (1) TNSN05187A1 (pt)
WO (1) WO2004070030A1 (pt)
ZA (1) ZA200506196B (pt)

Families Citing this family (11)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP5648912B2 (ja) * 2008-02-27 2015-01-07 国立大学法人北海道大学 動物細胞を用いて外来遺伝子由来タンパク質を大量に生産するための発現ベクター、およびその利用
JP5396071B2 (ja) 2008-12-05 2014-01-22 独立行政法人海洋研究開発機構 細胞の増殖を抑制する方法
US9096878B2 (en) * 2008-12-22 2015-08-04 National University Corporation Hokkaido University Expression vector for producing protein derived from foreign gene in large quantity using animal cells, and use thereof
DK2462237T3 (da) * 2009-08-06 2016-03-29 Cmc Icos Biolog Inc Fremgangsmåder til forbedring af rekombinant proteinekspression
CN103391998A (zh) * 2010-07-26 2013-11-13 Adv生物制剂公司 用于重组蛋白表达的载体和方法
CN102559734B (zh) * 2010-12-24 2014-10-29 神州细胞工程有限公司 一种可用于外源基因表达的载体及细胞株筛选方法
WO2014189071A1 (ja) 2013-05-22 2014-11-27 独立行政法人国立精神・神経医療研究センター 移植用幹細胞及びその製造方法
CN104694568B (zh) * 2013-12-10 2018-03-09 深圳先进技术研究院 动物细胞用表达载体、其制备方法及应用
US10006047B2 (en) 2015-02-13 2018-06-26 The Board Of Trustees Of The Leland Stanford Junior University Non-silencing selectable marker genes and methods of use
KR102616820B1 (ko) 2017-03-17 2023-12-21 애드베룸 바이오테크놀로지스, 인코포레이티드 향상된 유전자 발현을 위한 조성물 및 방법
CN114250226B (zh) * 2020-09-25 2026-08-11 北京安龙生物医药有限公司 用于高水平表达外源基因的表达载体

Family Cites Families (8)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
NZ210501A (en) * 1983-12-13 1991-08-27 Kirin Amgen Inc Erythropoietin produced by procaryotic or eucaryotic expression of an exogenous dna sequence
AU582288B2 (en) * 1986-03-07 1989-03-16 Damon Biotech Inc. Vector and method for achieving high level expression in eukaryotic cells
CA1339413C (en) * 1988-06-24 1997-09-02 Junichi Miyazaki Exogenous gene expression vector containing chick .beta.-actin gene promoter
CA2076975A1 (en) * 1990-02-28 1991-08-29 Kevin W. Moore Mammalian expression vectors
JPH04218383A (ja) * 1990-05-14 1992-08-07 Green Cross Corp:The プラスミド、形質転換された動物細胞及び異種蛋白質の製造方法
CA2078721A1 (en) * 1991-09-24 1993-03-25 Hiroshi Yonemura Process for preparing human coagulation factor viii protein complex
DE69636536T2 (de) * 1995-02-03 2007-10-25 Asahi Glass Co., Ltd. Sekretionssignal-Gen und dieses enthaltender Expressionsvektor
JP3582965B2 (ja) * 1996-08-23 2004-10-27 株式会社イムノ・ジャパン 哺乳動物細胞用発現ベクター

Also Published As

Publication number Publication date
CN1771324A (zh) 2006-05-10
TNSN05187A1 (en) 2007-06-11
CA2514941A1 (en) 2004-08-19
JPWO2004070030A1 (ja) 2006-05-25
MXPA05008231A (es) 2006-01-17
EP1591523A1 (en) 2005-11-02
WO2004070030A1 (ja) 2004-08-19
EP1591523A4 (en) 2007-07-11
AR043017A1 (es) 2005-07-13
ZA200506196B (en) 2006-10-25

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Legal Events

Date Code Title Description
B08F Application fees: dismissal - article 86 of industrial property law

Free format text: REFERENTE AS 6A, 7A E 8A ANUIDADES.

B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2159 DE 22/05/2012.

B15K Others concerning applications: alteration of classification

Free format text: PROCEDIMENTO AUTOMATICO DE RECLASSIFICACAO. AS CLASSIFICACOES IPC ANTERIORES ERAM: C12N 15/09; C12N 5/06.

Ipc: C12N 15/85 (2006.01)

Ipc: C12N 15/85 (2006.01)