BRPI0409239A - contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis - Google Patents

contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis

Info

Publication number
BRPI0409239A
BRPI0409239A BRPI0409239-2A BRPI0409239A BRPI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A
Authority
BR
Brazil
Prior art keywords
dna
commercially viable
production
improve
methods
Prior art date
Application number
BRPI0409239-2A
Other languages
English (en)
Inventor
Larry Gilbertson
Elysia Krieger
Wanggen Zhang
Xudong Ye
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0409239A publication Critical patent/BRPI0409239A/pt
Publication of BRPI0409239B1 publication Critical patent/BRPI0409239B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"CONSTRUCTOS DE DNA E MéTODOS PARA MELHORAR A PRODUçãO DE PLANTAS TRANSGêNICAS COMERCIALMENTE VIáVEIS". A presente invenção refere-se a um gene marcador selecionável negativo não-letal no DNA do esqueleto do vetor de um plasmídeo de DNA usado para transformar células de plantas. Estes transgenes são projetados para expressar um produto gênico não-letal em células de plantas que contém o DNA do esqueleto do vetor do plasmídeo de DNA. Os produtos gênicos do gene marcador selecionável negativo não-letal estão envolvidos nas vias de biossíntese de hormónio de planta, diversão de substrato de hormónio de planta, degradação de hormónio de planta, sinalização de hormónio de planta ou interferência metabólica. O uso destes plasmídeos de DNA para transformar células de plantas provê uma melhorada produção de plantas comercialmente viáveis.
BRPI0409239A 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta BRPI0409239B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46145903P 2003-04-09 2003-04-09
PCT/US2004/011000 WO2004092390A2 (en) 2003-04-09 2004-04-09 Dna constructs and methods to enhance the production of commercially viable transgenic plants

Publications (2)

Publication Number Publication Date
BRPI0409239A true BRPI0409239A (pt) 2006-03-28
BRPI0409239B1 BRPI0409239B1 (pt) 2020-01-14

Family

ID=33299814

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0409239A BRPI0409239B1 (pt) 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta

Country Status (11)

Country Link
US (2) US7575917B2 (pt)
EP (1) EP1613755B1 (pt)
JP (1) JP4744436B2 (pt)
CN (1) CN1809639B (pt)
AT (1) ATE471378T1 (pt)
AU (1) AU2004230602B2 (pt)
BR (1) BRPI0409239B1 (pt)
CA (2) CA2870964A1 (pt)
DE (1) DE602004027717D1 (pt)
MX (1) MXPA05010872A (pt)
WO (1) WO2004092390A2 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060041956A1 (en) * 2004-04-06 2006-02-23 Pioneer Hi-Bred International, Inc. Vectors for plant transformation and methods of use
US8993846B2 (en) * 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
PL1989315T3 (pl) * 2006-02-06 2015-10-30 Simplot Co J R Wytwarzanie wolnych od markera i wolnych od szkieletu roślin transgenicznych przy użyciu podejścia z pojedynczym wektorem binarnym
WO2007134234A2 (en) * 2006-05-12 2007-11-22 Monsanto Technology Llc Methods and compositions for obtaining marker-free transgenic plants
US8395020B2 (en) 2006-08-31 2013-03-12 Monsanto Technology Llc Methods for rapidly transforming monocots
EP3023499A1 (en) 2008-07-16 2016-05-25 Monsanto Technology LLC Methods and vectors for producing transgenic plants
JP5769698B2 (ja) 2009-03-30 2015-08-26 モンサント テクノロジー エルエルシー 遺伝子組換えイネ事象１７３１４およびその使用方法
BR112014013853A2 (pt) 2011-12-09 2020-01-14 Ceres, Inc. uso de um ácido nucleico isolado, método de obtenção de uma célula vegetal transgênica, método de obtenção de uma planta transgênica, método de produção de uma planta, método de produção de biomassa, método de processamento de biomassa, método de alteração da composição de biomassa, método de modulação de composição de biomassa, método de produção de um produto de forragem
AU2013274542B2 (en) 2012-06-12 2017-03-30 Syngenta Crop Protection Ag Methods and compositions for determination of vector backbone in a nucleic acid sample
CA2911185C (en) 2013-06-11 2023-09-05 Syngenta Participations Ag Methods for generating transgenic plants
JPWO2015118640A1 (ja) 2014-02-06 2017-03-23 日本たばこ産業株式会社 植物の形質転換細胞の取得方法
EP3356537B1 (en) 2015-10-02 2022-08-17 Monsanto Technology LLC Recombinant maize b chromosome sequence and uses thereof
US20180265877A1 (en) 2015-10-16 2018-09-20 Pioneer Hi-Bred International, Inc. Methods and compositions for agrobacterium comprising negative selection markers
US11732268B2 (en) 2016-06-28 2023-08-22 Monsanto Technology Llc Methods and compositions for use in genome modification in plants
WO2019161143A1 (en) 2018-02-15 2019-08-22 Monsanto Technology Llc Improved methods for hybrid corn seed production
US12274205B2 (en) 2018-12-12 2025-04-15 Monsanto Technology Llc Delayed harvest of short stature corn plants
WO2021022043A2 (en) * 2019-07-30 2021-02-04 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
CN116829707A (zh) 2020-11-11 2023-09-29 孟山都技术公司 提高定点整合频率的方法
US20220159905A1 (en) 2020-11-23 2022-05-26 Monsanto Technology Llc Management of corn through semi-dwarf systems

Family Cites Families (15)

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Publication number Priority date Publication date Assignee Title
CA2096637A1 (en) * 1990-12-26 1992-06-27 Harry J. Klee Control of fruit ripening and senescence in plants
GB9311147D0 (en) * 1993-05-28 1993-07-14 Long Ashton Research Station Regulation of plant growth
JP3256952B2 (ja) 1994-11-09 2002-02-18 日本製紙株式会社 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法
WO1997014807A1 (en) 1995-10-16 1997-04-24 Seminis Vegetable Seeds, Inc. A method for visually selecting transgenic plant cells or tissues by carotenoid pigmentation
CN1192110C (zh) 1997-06-30 2005-03-09 辛根塔默根有限公司 用于植物转化的质粒和使用此质粒的方法
US6521458B1 (en) * 1998-05-22 2003-02-18 Dna Plant Technology Corporation Compositions and methods for improved plant transformation
WO2000037060A2 (en) 1998-12-22 2000-06-29 National Research Council Of Canada Transgenic plants comprising a conditionally lethal gene
JP2001218583A (ja) * 1999-01-06 2001-08-14 Nippon Paper Industries Co Ltd 新規選抜マーカーを用いた植物への遺伝子導入用ベクター
CA2292798A1 (en) 1999-01-06 2000-07-06 Nippon Paper Industries Co., Ltd. Novel vector for introducing a gene into a plant using a selectable marker
JP4080746B2 (ja) 1999-12-16 2008-04-23 クロップデザイン・エヌ・ヴェー 最適化されたｔ−ｄｎａ転移およびそのためのベクター
AU2001247480A1 (en) * 2000-03-17 2001-10-03 Exelixis Plant Sciences, Inc. Melon promoters for expression of transgenes in plants
EP2192176A1 (en) * 2000-06-16 2010-06-02 Schmülling, Thomas Method for modifying plant morphology, biochemistry and physiology
EP1294907B1 (de) * 2000-06-28 2008-10-29 Sungene GmbH & Co. KGaA Binärvektoren zur verbesserten transformation von pflanzlichen systemen
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US7598430B2 (en) 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation

Also Published As

Publication number Publication date
DE602004027717D1 (de) 2010-07-29
CN1809639B (zh) 2011-05-04
CA2521674A1 (en) 2004-10-28
US7575917B2 (en) 2009-08-18
EP1613755A2 (en) 2006-01-11
JP2006522610A (ja) 2006-10-05
MXPA05010872A (es) 2006-01-23
ATE471378T1 (de) 2010-07-15
CA2870964A1 (en) 2004-10-28
AU2004230602A1 (en) 2004-10-28
WO2004092390A3 (en) 2005-01-20
US8415527B2 (en) 2013-04-09
EP1613755B1 (en) 2010-06-16
US20090328253A1 (en) 2009-12-31
JP4744436B2 (ja) 2011-08-10
CA2521674C (en) 2015-02-24
AU2004230602B2 (en) 2009-07-09
BRPI0409239B1 (pt) 2020-01-14
CN1809639A (zh) 2006-07-26
US20040237142A1 (en) 2004-11-25
WO2004092390A2 (en) 2004-10-28

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B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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B12B Appeal against refusal [chapter 12.2 patent gazette]
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Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 14/01/2020, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

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B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2665 DE 01-02-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.