BRPI0409239A - contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis - Google Patents

contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis

Info

Publication number
BRPI0409239A
BRPI0409239A BRPI0409239-2A BRPI0409239A BRPI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A
Authority
BR
Brazil
Prior art keywords
dna
commercially viable
production
improve
methods
Prior art date
Application number
BRPI0409239-2A
Other languages
English (en)
Inventor
Larry Gilbertson
Elysia Krieger
Wanggen Zhang
Xudong Ye
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0409239A publication Critical patent/BRPI0409239A/pt
Publication of BRPI0409239B1 publication Critical patent/BRPI0409239B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"CONSTRUCTOS DE DNA E MéTODOS PARA MELHORAR A PRODUçãO DE PLANTAS TRANSGêNICAS COMERCIALMENTE VIáVEIS". A presente invenção refere-se a um gene marcador selecionável negativo não-letal no DNA do esqueleto do vetor de um plasmídeo de DNA usado para transformar células de plantas. Estes transgenes são projetados para expressar um produto gênico não-letal em células de plantas que contém o DNA do esqueleto do vetor do plasmídeo de DNA. Os produtos gênicos do gene marcador selecionável negativo não-letal estão envolvidos nas vias de biossíntese de hormónio de planta, diversão de substrato de hormónio de planta, degradação de hormónio de planta, sinalização de hormónio de planta ou interferência metabólica. O uso destes plasmídeos de DNA para transformar células de plantas provê uma melhorada produção de plantas comercialmente viáveis.
BRPI0409239A 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta BRPI0409239B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46145903P 2003-04-09 2003-04-09
PCT/US2004/011000 WO2004092390A2 (en) 2003-04-09 2004-04-09 Dna constructs and methods to enhance the production of commercially viable transgenic plants

Publications (2)

Publication Number Publication Date
BRPI0409239A true BRPI0409239A (pt) 2006-03-28
BRPI0409239B1 BRPI0409239B1 (pt) 2020-01-14

Family

ID=33299814

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0409239A BRPI0409239B1 (pt) 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta

Country Status (11)

Country Link
US (2) US7575917B2 (pt)
EP (1) EP1613755B1 (pt)
JP (1) JP4744436B2 (pt)
CN (1) CN1809639B (pt)
AT (1) ATE471378T1 (pt)
AU (1) AU2004230602B2 (pt)
BR (1) BRPI0409239B1 (pt)
CA (2) CA2521674C (pt)
DE (1) DE602004027717D1 (pt)
MX (1) MXPA05010872A (pt)
WO (1) WO2004092390A2 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060041956A1 (en) * 2004-04-06 2006-02-23 Pioneer Hi-Bred International, Inc. Vectors for plant transformation and methods of use
US8993846B2 (en) 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
NZ570698A (en) 2006-02-06 2012-02-24 Simplot Co J R Generation of marker-free and backbone-free transgenic plants using Agrobacterium containing a plasmid comprising a transfer DNA gene and a plant hormone biosynthesis gene
AU2007249224B2 (en) 2006-05-12 2013-03-21 Monsanto Technology Llc Methods and compositions for obtaining marker-free transgenic plants
AU2007289109B2 (en) * 2006-08-31 2012-11-15 Monsanto Technology Llc. Methods for rapidly transforming monocots
EP3023499A1 (en) 2008-07-16 2016-05-25 Monsanto Technology LLC Methods and vectors for producing transgenic plants
KR101813722B1 (ko) 2009-03-30 2017-12-29 몬산토 테크놀로지 엘엘씨 벼의 17314 트랜스제닉 사건 및 이의 이용 방법
US10323256B2 (en) 2011-12-09 2019-06-18 Ceres, Inc. Transgenic plants having altered biomass composition
AU2013274542B2 (en) 2012-06-12 2017-03-30 Syngenta Crop Protection Ag Methods and compositions for determination of vector backbone in a nucleic acid sample
CA2911185C (en) 2013-06-11 2023-09-05 Syngenta Participations Ag Methods for generating transgenic plants
WO2015118640A1 (ja) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 植物の形質転換細胞の取得方法
WO2017059341A1 (en) 2015-10-02 2017-04-06 Monsanto Technology Llc Recombinant maize b chromosome sequence and uses thereof
BR112018007684A2 (pt) 2015-10-16 2018-11-06 Du Pont bactéria de transformação de planta da ordem rhizobiales, método para transferir sequências nucleotídicas selecionadas a uma planta, método para remover uma bactéria de transformação de planta da ordem rhizobiales, método para transformar uma célula vegetal e método para contrasselecionar contra bactérias de transformação de planta da ordem rhizobiales
WO2018005491A1 (en) 2016-06-28 2018-01-04 Monsanto Technology Llc Methods and compositions for use in genome modification in plants
CN111954463A (zh) 2018-02-15 2020-11-17 孟山都技术公司 用于杂交玉米种子生产的改进方法
US12274205B2 (en) 2018-12-12 2025-04-15 Monsanto Technology Llc Delayed harvest of short stature corn plants
WO2021022043A2 (en) * 2019-07-30 2021-02-04 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
CN116829707A (zh) 2020-11-11 2023-09-29 孟山都技术公司 提高定点整合频率的方法
US20220162632A1 (en) 2020-11-23 2022-05-26 Monsanto Technology Llc Delayed harvest of short stature corn plants

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FI932960A7 (fi) * 1990-12-26 1993-06-24 Monsanto Co Hedelmien kypsymisen ja kasvien vanhenemisen kontrollointi
GB9311147D0 (en) * 1993-05-28 1993-07-14 Long Ashton Research Station Regulation of plant growth
JP3256952B2 (ja) * 1994-11-09 2002-02-18 日本製紙株式会社 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法
AU5377896A (en) 1995-10-16 1997-05-07 Seminis Vegetable Seeds, Inc. A method for visually selecting transgenic plant cells or tissues by carotenoid pigmentation
BR9810368A (pt) * 1997-06-30 2000-08-29 Mogen Int Plasmìdeos para transformação de plantas e processo para usar os mesmos
US6521458B1 (en) 1998-05-22 2003-02-18 Dna Plant Technology Corporation Compositions and methods for improved plant transformation
CN1219885C (zh) 1998-12-22 2005-09-21 加拿大国家研究委员会 转基因植物及其生产方法
CA2292798A1 (en) 1999-01-06 2000-07-06 Nippon Paper Industries Co., Ltd. Novel vector for introducing a gene into a plant using a selectable marker
JP2001218583A (ja) * 1999-01-06 2001-08-14 Nippon Paper Industries Co Ltd 新規選抜マーカーを用いた植物への遺伝子導入用ベクター
AU782327C (en) 1999-12-16 2006-09-14 Cropdesign N.V. Optimized T-DNA transfer and vectors therefor
WO2001071013A2 (en) * 2000-03-17 2001-09-27 Exelixis Plant Sciences, Inc. Melon promoters for expression of transgenes in plants
CN102174540B (zh) * 2000-06-16 2013-08-07 托马斯·施穆林 改变植物形态学、生物化学和生理学的方法
AU2001277520B2 (en) * 2000-06-28 2006-08-03 Sungene Gmbh & Co. Kgaa Binary vectors for improved transformation of plant systems
AU2003219806B8 (en) 2002-02-20 2011-01-27 J.R. Simplot Company Precise breeding
US7598430B2 (en) * 2002-03-20 2009-10-06 J.R. Simplot Company Refined plant transformation

Also Published As

Publication number Publication date
CA2521674C (en) 2015-02-24
EP1613755B1 (en) 2010-06-16
US20090328253A1 (en) 2009-12-31
WO2004092390A2 (en) 2004-10-28
BRPI0409239B1 (pt) 2020-01-14
CN1809639A (zh) 2006-07-26
AU2004230602B2 (en) 2009-07-09
US7575917B2 (en) 2009-08-18
ATE471378T1 (de) 2010-07-15
US20040237142A1 (en) 2004-11-25
JP2006522610A (ja) 2006-10-05
DE602004027717D1 (de) 2010-07-29
MXPA05010872A (es) 2006-01-23
JP4744436B2 (ja) 2011-08-10
WO2004092390A3 (en) 2005-01-20
EP1613755A2 (en) 2006-01-11
CA2521674A1 (en) 2004-10-28
US8415527B2 (en) 2013-04-09
CN1809639B (zh) 2011-05-04
AU2004230602A1 (en) 2004-10-28
CA2870964A1 (en) 2004-10-28

Similar Documents

Publication Publication Date Title
BRPI0409239A (pt) contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis
Eichten et al. Epigenetics: beyond chromatin modifications and complex genetic regulation
WO2019103442A3 (ko) CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도
FI3472325T3 (fi) Menetelmä kohdennettuun dna:n muuttamiseen kasvisoluissa
UY32066A (es) Construcciones de adn recombinante y métodos para modular la expresión de un gen diana
UA103887C2 (ru) Способ получения стойкого к гербицидам растения
UA118535C2 (uk) Рекомбінантна молекула днк, що надає рослині brassica стійкості до гліфосату
PH12017500576B1 (en) Plant regulatory elements and uses thereof
MY176922A (en) Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
WO2010014857A3 (en) Chromosome therapy
MX2013007678A (es) Moleculas de acido nucleico dirigidas ala pequeña proteina de union de gtp rho1 y que confieren resistencia a las plagas de coleopteros.
MX2009013422A (es) Metodos de mejoramiento molecular dirigidos a secuencias.
WO2019140102A8 (en) Compositions and methods for facilitating delivery of synthetic nucleic acids to cells
MX2022006938A (es) Elementos reguladores de las plantas y sus usos.
ZA202101024B (en) Insecticidal proteins and methods for their use
NZ700902A (en) Lowering saturated fatty acid content of plant seeds
MX347575B (es) Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales.
AR068847A1 (es) Genes sinteticos que codifican cry1ac
MX2021014004A (es) Elementos reguladores de plantas y usos de los mismos.
PH12018501939A1 (en) Plant regulatory elements and uses thereof
PH12021550220A1 (en) Plant regulatory elements and uses thereof
WO2015164726A3 (en) Chimeric vsv-g proteins as nucleic acid transfer vehicles
WO2020030783A3 (en) Means and methods for drought tolerance in crops
NZ748198A (en) Plant regulatory elements and uses thereof
PH12017500612A1 (en) Gho/sec24b2 and sec24b1 nucleic acid molecules to control coleopteran and hemipteran pests

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24 E 25 DA LPI.

B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 14/01/2020, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 18A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2665 DE 01-02-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.