BRPI0409239A - contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis - Google Patents

contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis

Info

Publication number
BRPI0409239A
BRPI0409239A BRPI0409239-2A BRPI0409239A BRPI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A BR PI0409239 A BRPI0409239 A BR PI0409239A
Authority
BR
Brazil
Prior art keywords
dna
commercially viable
production
improve
methods
Prior art date
Application number
BRPI0409239-2A
Other languages
English (en)
Inventor
Larry Gilbertson
Elysia Krieger
Wanggen Zhang
Xudong Ye
Original Assignee
Monsanto Technology Llc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Monsanto Technology Llc filed Critical Monsanto Technology Llc
Publication of BRPI0409239A publication Critical patent/BRPI0409239A/pt
Publication of BRPI0409239B1 publication Critical patent/BRPI0409239B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8201—Methods for introducing genetic material into plant cells, e.g. DNA, RNA, stable or transient incorporation, tissue culture methods adapted for transformation
    • C12N15/8209—Selection, visualisation of transformants, reporter constructs, e.g. antibiotic resistance markers
    • C12N15/821—Non-antibiotic resistance markers, e.g. morphogenetic, metabolic markers
    • C12N15/8212—Colour markers, e.g. beta-glucoronidase [GUS], green fluorescent protein [GFP], carotenoid

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"CONSTRUCTOS DE DNA E MéTODOS PARA MELHORAR A PRODUçãO DE PLANTAS TRANSGêNICAS COMERCIALMENTE VIáVEIS". A presente invenção refere-se a um gene marcador selecionável negativo não-letal no DNA do esqueleto do vetor de um plasmídeo de DNA usado para transformar células de plantas. Estes transgenes são projetados para expressar um produto gênico não-letal em células de plantas que contém o DNA do esqueleto do vetor do plasmídeo de DNA. Os produtos gênicos do gene marcador selecionável negativo não-letal estão envolvidos nas vias de biossíntese de hormónio de planta, diversão de substrato de hormónio de planta, degradação de hormónio de planta, sinalização de hormónio de planta ou interferência metabólica. O uso destes plasmídeos de DNA para transformar células de plantas provê uma melhorada produção de plantas comercialmente viáveis.
BRPI0409239A 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta BRPI0409239B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US46145903P 2003-04-09 2003-04-09
PCT/US2004/011000 WO2004092390A2 (en) 2003-04-09 2004-04-09 Dna constructs and methods to enhance the production of commercially viable transgenic plants

Publications (2)

Publication Number Publication Date
BRPI0409239A true BRPI0409239A (pt) 2006-03-28
BRPI0409239B1 BRPI0409239B1 (pt) 2020-01-14

Family

ID=33299814

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0409239A BRPI0409239B1 (pt) 2003-04-09 2004-04-09 plasmídeo de dna compreendendo gene fitoeno sintase, bem como métodos para melhorar a seleção de plantas transgênicas e para reduzir número de cópias de transgene em célula de planta

Country Status (11)

Country Link
US (2) US7575917B2 (pt)
EP (1) EP1613755B1 (pt)
JP (1) JP4744436B2 (pt)
CN (1) CN1809639B (pt)
AT (1) ATE471378T1 (pt)
AU (1) AU2004230602B2 (pt)
BR (1) BRPI0409239B1 (pt)
CA (2) CA2870964A1 (pt)
DE (1) DE602004027717D1 (pt)
MX (1) MXPA05010872A (pt)
WO (1) WO2004092390A2 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20060041956A1 (en) * 2004-04-06 2006-02-23 Pioneer Hi-Bred International, Inc. Vectors for plant transformation and methods of use
US8993846B2 (en) 2005-09-06 2015-03-31 Monsanto Technology Llc Vectors and methods for improved plant transformation efficiency
US7923600B2 (en) * 2006-02-06 2011-04-12 J.R. Simplot Company Generation of marker-free and backbone-free transgenic plants using a single binary approach
JP5330231B2 (ja) * 2006-05-12 2013-10-30 モンサント テクノロジー エルエルシー マーカーフリーのトランスジェニック植物を得るための方法および組成物
DE602007012269D1 (de) 2006-08-31 2011-03-10 Monsanto Technology Llc Verfahren zur herstellung transgener pflanzen
EP2300617B1 (en) 2008-07-16 2016-03-23 Monsanto Technology LLC Methods and vectors for producing transgenic plants
JP5769698B2 (ja) 2009-03-30 2015-08-26 モンサント テクノロジー エルエルシー 遺伝子組換えイネ事象１７３１４およびその使用方法
WO2013086499A2 (en) 2011-12-09 2013-06-13 Ceres, Inc. Transgenic plants having altered biomass composition
US9797004B2 (en) 2012-06-12 2017-10-24 Syngenta Participations Ag Methods and compositions for determination of vector backbone in a nucleic acid sample
AU2014278519B2 (en) 2013-06-11 2020-09-10 Syngenta Crop Protection Ag Methods for generating transgenic plants
WO2015118640A1 (ja) * 2014-02-06 2015-08-13 日本たばこ産業株式会社 植物の形質転換細胞の取得方法
WO2017059341A1 (en) 2015-10-02 2017-04-06 Monsanto Technology Llc Recombinant maize b chromosome sequence and uses thereof
EP3362568A1 (en) 2015-10-16 2018-08-22 Pioneer Hi-Bred International, Inc. Methods and compositions for transformation comprising negative selection markers
US11732268B2 (en) 2016-06-28 2023-08-22 Monsanto Technology Llc Methods and compositions for use in genome modification in plants
CA3090007A1 (en) 2018-02-15 2019-08-22 Monsanto Technology Llc Improved methods for hybrid corn seed production
US12274205B2 (en) 2018-12-12 2025-04-15 Monsanto Technology Llc Delayed harvest of short stature corn plants
WO2021022043A2 (en) * 2019-07-30 2021-02-04 Pairwise Plants Services, Inc. Morphogenic regulators and methods of using the same
JP2023550323A (ja) 2020-11-11 2023-12-01 モンサント テクノロジー エルエルシー 部位特異的組込み頻度を改善するための方法
US20220162632A1 (en) 2020-11-23 2022-05-26 Monsanto Technology Llc Delayed harvest of short stature corn plants

Family Cites Families (15)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
BR9107191A (pt) * 1990-12-26 1994-06-14 Monsanto Co Controle de amadurecimento de frutos e senescência em plantas
GB9311147D0 (en) * 1993-05-28 1993-07-14 Long Ashton Research Station Regulation of plant growth
JP3256952B2 (ja) 1994-11-09 2002-02-18 日本製紙株式会社 植物への遺伝子導入用ベクター、並びにこれを用いた遺伝子導入植物の作成方法及び植物への遣伝子多重導入方法
WO1997014807A1 (en) 1995-10-16 1997-04-24 Seminis Vegetable Seeds, Inc. A method for visually selecting transgenic plant cells or tissues by carotenoid pigmentation
CN1192110C (zh) * 1997-06-30 2005-03-09 辛根塔默根有限公司 用于植物转化的质粒和使用此质粒的方法
US6521458B1 (en) 1998-05-22 2003-02-18 Dna Plant Technology Corporation Compositions and methods for improved plant transformation
AU776046B2 (en) 1998-12-22 2004-08-26 Dow Agrosciences Llc Transgenic plants and methods for production thereof
JP2001218583A (ja) * 1999-01-06 2001-08-14 Nippon Paper Industries Co Ltd 新規選抜マーカーを用いた植物への遺伝子導入用ベクター
CA2292798A1 (en) * 1999-01-06 2000-07-06 Nippon Paper Industries Co., Ltd. Novel vector for introducing a gene into a plant using a selectable marker
ATE447036T1 (de) 1999-12-16 2009-11-15 Cropdesign Nv Optimisierter t-dna transfer und entsprechende vektoren
AU2001247480A1 (en) * 2000-03-17 2001-10-03 Exelixis Plant Sciences, Inc. Melon promoters for expression of transgenes in plants
DE60139619D1 (de) * 2000-06-16 2009-10-01 Thomas Schmuelling Verfahren zur modifizierung der morphologie, biochemien und physiologie der pflanzen durch cytokinin-oxidase
AU7752001A (en) * 2000-06-28 2002-01-08 Sungene Gmbh And Co Kgaa Binary vectors for improved transformation of plant systems
NZ535395A (en) 2002-02-20 2009-01-31 Simplot Co J R Precise plant breeding using plant DNA border like sequences instead of Agrobacterium borders
EP1560484B1 (en) 2002-03-20 2011-05-11 J.R. Simplot Company Refined plant transformation

Also Published As

Publication number Publication date
US20090328253A1 (en) 2009-12-31
EP1613755A2 (en) 2006-01-11
US20040237142A1 (en) 2004-11-25
US8415527B2 (en) 2013-04-09
AU2004230602B2 (en) 2009-07-09
AU2004230602A1 (en) 2004-10-28
CA2521674C (en) 2015-02-24
DE602004027717D1 (de) 2010-07-29
BRPI0409239B1 (pt) 2020-01-14
CN1809639B (zh) 2011-05-04
CA2870964A1 (en) 2004-10-28
JP2006522610A (ja) 2006-10-05
CN1809639A (zh) 2006-07-26
WO2004092390A3 (en) 2005-01-20
CA2521674A1 (en) 2004-10-28
JP4744436B2 (ja) 2011-08-10
EP1613755B1 (en) 2010-06-16
US7575917B2 (en) 2009-08-18
WO2004092390A2 (en) 2004-10-28
MXPA05010872A (es) 2006-01-23
ATE471378T1 (de) 2010-07-15

Similar Documents

Publication Publication Date Title
BRPI0409239A (pt) contructos de dna e métodos para melhorar a produção de plantas transgênicas comercialmente viáveis
Eichten et al. Epigenetics: beyond chromatin modifications and complex genetic regulation
WO2019103442A3 (ko) CRISPR/Cpf1 시스템을 이용한 유전체 편집용 조성물 및 이의 용도
FI3472325T3 (fi) Menetelmä kohdennettuun dna:n muuttamiseen kasvisoluissa
EP4310196A3 (en) Methods and compositions for manipulating nucleic acids
UA103887C2 (ru) Способ получения стойкого к гербицидам растения
MX347199B (es) Evento de brassica transgénica mon 88302 y métodos para su uso.
MY176922A (en) Engineered zinc finger proteins targeting plant genes involved in fatty acid biosynthesis
WO2010014857A3 (en) Chromosome therapy
MX2013007678A (es) Moleculas de acido nucleico dirigidas ala pequeña proteina de union de gtp rho1 y que confieren resistencia a las plagas de coleopteros.
WO2019140102A8 (en) Compositions and methods for facilitating delivery of synthetic nucleic acids to cells
MX344605B (es) Elementos de regulacion de plantas y sus usos.
MX2022006939A (es) Elementos reguladores de las plantas y sus usos.
ZA202101024B (en) Insecticidal proteins and methods for their use
MX347575B (es) Metodos y medios para modificar un genoma vegetal en una secuencia de nucleotidos usada comunmente en la ingenieria genetica de genomas vegetales.
MX2021014004A (es) Elementos reguladores de plantas y usos de los mismos.
WO2020077347A3 (en) Compositions and methods for transfecting cells
PH12018501939A1 (en) Plant regulatory elements and uses thereof
PH12021550220A1 (en) Plant regulatory elements and uses thereof
WO2015164726A3 (en) Chimeric vsv-g proteins as nucleic acid transfer vehicles
WO2020030783A3 (en) Means and methods for drought tolerance in crops
PH12018502447B1 (en) Plant regulatory elements and uses thereof
PH12017500612A1 (en) Gho/sec24b2 and sec24b1 nucleic acid molecules to control coleopteran and hemipteran pests
WO2019150200A3 (en) Dna free crispr plant transformation
WO2017066041A3 (en) Wupa nucleic acid molecules that confer resistance to coleopteran and hemipteran pests

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 24 E 25 DA LPI.

B12B Appeal against refusal [chapter 12.2 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 14/01/2020, OBSERVADAS AS CONDICOES LEGAIS.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 18A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2665 DE 01-02-2022 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.