BRPI0414422A - método para a análise de assinatura de identificação de gene (gis) - Google Patents

método para a análise de assinatura de identificação de gene (gis)

Info

Publication number
BRPI0414422A
BRPI0414422A BRPI0414422-8A BRPI0414422A BRPI0414422A BR PI0414422 A BRPI0414422 A BR PI0414422A BR PI0414422 A BRPI0414422 A BR PI0414422A BR PI0414422 A BRPI0414422 A BR PI0414422A
Authority
BR
Brazil
Prior art keywords
gis
identification signature
label
signature analysis
gene identification
Prior art date
Application number
BRPI0414422-8A
Other languages
English (en)
Inventor
Yijun Ruan
Patrick Ng
Chialin Wei
Original Assignee
Agency Science Tech & Res
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Agency Science Tech & Res filed Critical Agency Science Tech & Res
Publication of BRPI0414422A publication Critical patent/BRPI0414422A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/02—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with ribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1065—Preparation or screening of tagged libraries, e.g. tagged microorganisms by STM-mutagenesis, tagged polynucleotides, gene tags
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1093—General methods of preparing gene libraries, not provided for in other subgroups
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1096—Processes for the isolation, preparation or purification of DNA or RNA cDNA Synthesis; Subtracted cDNA library construction, e.g. RT, RT-PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6809—Methods for determination or identification of nucleic acids involving differential detection
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2521/00—Reaction characterised by the enzymatic activity
    • C12Q2521/30—Phosphoric diester hydrolysing, i.e. nuclease
    • C12Q2521/313—Type II endonucleases, i.e. cutting outside recognition site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/131—Modifications characterised by incorporating a restriction site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/191—Modifications characterised by incorporating an adaptor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Plant Pathology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODO PARA A ANáLISE DE ASSINATURA DE IDENTIFICAçãO DE GENE (GIS)". Um oligonucleotídeo isolado que compreende pelo menos um rótulo duplo, em que o rótulo duplo compreende dois rótulos de seqüência, primeiro e segundo, unidos, em que o primeiro rótulo compreende a seqüência de terminal 5<39> e o segundo rótulo compreende a seqüência de terminal 3<39> de uma molécula de ácido nucléico ou um fragmento deste. A análise do rótulo duplo é útil para o gene descoberto e o mapeamento do genoma.
BRPI0414422-8A 2003-09-17 2004-09-16 método para a análise de assinatura de identificação de gene (gis) BRPI0414422A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/664,234 US8222005B2 (en) 2003-09-17 2003-09-17 Method for gene identification signature (GIS) analysis
PCT/SG2004/000298 WO2005026391A1 (en) 2003-09-17 2004-09-16 Method for gene identification signature (gis) analysis

Publications (1)

Publication Number Publication Date
BRPI0414422A true BRPI0414422A (pt) 2006-11-14

Family

ID=34274547

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0414422-8A BRPI0414422A (pt) 2003-09-17 2004-09-16 método para a análise de assinatura de identificação de gene (gis)

Country Status (12)

Country Link
US (4) US8222005B2 (pt)
EP (1) EP1533386B1 (pt)
JP (2) JP4371967B2 (pt)
KR (1) KR20060072145A (pt)
CN (1) CN1849401B (pt)
AU (1) AU2004272950A1 (pt)
BR (1) BRPI0414422A (pt)
CA (1) CA2538137A1 (pt)
MY (1) MY141856A (pt)
SG (1) SG146647A1 (pt)
TW (1) TWI365222B (pt)
WO (1) WO2005026391A1 (pt)

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US8005621B2 (en) * 2004-09-13 2011-08-23 Agency For Science Technology And Research Transcript mapping method
US8428882B2 (en) * 2005-06-14 2013-04-23 Agency For Science, Technology And Research Method of processing and/or genome mapping of diTag sequences
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EP1951900B1 (en) * 2005-10-07 2016-06-15 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
US7960104B2 (en) 2005-10-07 2011-06-14 Callida Genomics, Inc. Self-assembled single molecule arrays and uses thereof
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US20090156431A1 (en) * 2007-12-12 2009-06-18 Si Lok Methods for Nucleic Acid Mapping and Identification of Fine Structural Variations in Nucleic Acids
ATE483804T1 (de) * 2006-01-04 2010-10-15 Si Lok Verfahren zur zuordnung von nukleinsäuren und zur identifikation fein strukturierter variationen in nukleinsäuren sowie hilfsmittel dafür
SG170028A1 (en) * 2006-02-24 2011-04-29 Callida Genomics Inc High throughput genome sequencing on dna arrays
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US9074244B2 (en) 2008-03-11 2015-07-07 Affymetrix, Inc. Array-based translocation and rearrangement assays
WO2010104608A2 (en) * 2009-03-13 2010-09-16 Life Technologies Corporation Computer implemented method for indexing reference genome
JP2012000044A (ja) * 2010-06-16 2012-01-05 Hitachi Ltd 大規模並列核酸分析方法
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EP4490318A4 (en) * 2022-03-07 2026-06-10 Twist Bioscience Corp METHYLATION-MEDIATED ADAPTER ELIMINATION ON NUCLEIC ACID SEQUENCES

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Also Published As

Publication number Publication date
US20050255501A1 (en) 2005-11-17
US8222005B2 (en) 2012-07-17
JP2005118036A (ja) 2005-05-12
US20050059022A1 (en) 2005-03-17
AU2004272950A1 (en) 2005-03-24
SG146647A1 (en) 2008-10-30
US20070161024A1 (en) 2007-07-12
CA2538137A1 (en) 2005-03-24
KR20060072145A (ko) 2006-06-27
EP1533386A3 (en) 2006-08-30
US7553947B2 (en) 2009-06-30
TW200517916A (en) 2005-06-01
EP1533386B1 (en) 2012-06-20
CN1849401A (zh) 2006-10-18
TWI365222B (en) 2012-06-01
CN1849401B (zh) 2010-08-18
US20060084111A1 (en) 2006-04-20
EP1533386A2 (en) 2005-05-25
WO2005026391A1 (en) 2005-03-24
JP2009273470A (ja) 2009-11-26
JP4371967B2 (ja) 2009-11-25
MY141856A (en) 2010-07-16

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Legal Events

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B08F Application fees: application dismissed [chapter 8.6 patent gazette]

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE 8.6 DA RPI 2112 DE 28/06/2011.