BRPI0414611A - método de clivagem de polipeptìdeo usando variante de protease ompt - Google Patents
método de clivagem de polipeptìdeo usando variante de protease omptInfo
- Publication number
- BRPI0414611A BRPI0414611A BRPI0414611-5A BRPI0414611A BRPI0414611A BR PI0414611 A BRPI0414611 A BR PI0414611A BR PI0414611 A BRPI0414611 A BR PI0414611A BR PI0414611 A BRPI0414611 A BR PI0414611A
- Authority
- BR
- Brazil
- Prior art keywords
- amino acid
- polypeptide
- ompt protease
- cleavage method
- protease variant
- Prior art date
Links
- 238000003776 cleavage reaction Methods 0.000 title abstract 5
- 229920001184 polypeptide Polymers 0.000 title abstract 5
- 102000004196 processed proteins & peptides Human genes 0.000 title abstract 5
- 108090000765 processed proteins & peptides Proteins 0.000 title abstract 5
- 230000007017 scission Effects 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 3
- 108010003052 omptin outer membrane protease Proteins 0.000 title abstract 3
- 150000001413 amino acids Chemical class 0.000 abstract 6
- 235000001014 amino acid Nutrition 0.000 abstract 5
- 239000004475 Arginine Substances 0.000 abstract 1
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- WHUUTDBJXJRKMK-UHFFFAOYSA-N Glutamic acid Natural products OC(=O)C(N)CCC(O)=O WHUUTDBJXJRKMK-UHFFFAOYSA-N 0.000 abstract 1
- ONIBWKKTOPOVIA-BYPYZUCNSA-N L-Proline Chemical compound OC(=O)[C@@H]1CCCN1 ONIBWKKTOPOVIA-BYPYZUCNSA-N 0.000 abstract 1
- CKLJMWTZIZZHCS-REOHCLBHSA-N L-aspartic acid Chemical compound OC(=O)[C@@H](N)CC(O)=O CKLJMWTZIZZHCS-REOHCLBHSA-N 0.000 abstract 1
- WHUUTDBJXJRKMK-VKHMYHEASA-N L-glutamic acid Chemical compound OC(=O)[C@@H](N)CCC(O)=O WHUUTDBJXJRKMK-VKHMYHEASA-N 0.000 abstract 1
- KDXKERNSBIXSRK-UHFFFAOYSA-N Lysine Natural products NCCCCC(N)C(O)=O KDXKERNSBIXSRK-UHFFFAOYSA-N 0.000 abstract 1
- 239000004472 Lysine Substances 0.000 abstract 1
- ONIBWKKTOPOVIA-UHFFFAOYSA-N Proline Natural products OC(=O)C1CCCN1 ONIBWKKTOPOVIA-UHFFFAOYSA-N 0.000 abstract 1
- ODKSFYDXXFIFQN-UHFFFAOYSA-N arginine Natural products OC(=O)C(N)CCCNC(N)=N ODKSFYDXXFIFQN-UHFFFAOYSA-N 0.000 abstract 1
- 235000003704 aspartic acid Nutrition 0.000 abstract 1
- OQFSQFPPLPISGP-UHFFFAOYSA-N beta-carboxyaspartic acid Natural products OC(=O)C(N)C(C(O)=O)C(O)=O OQFSQFPPLPISGP-UHFFFAOYSA-N 0.000 abstract 1
- 235000013922 glutamic acid Nutrition 0.000 abstract 1
- 239000004220 glutamic acid Substances 0.000 abstract 1
- 235000013930 proline Nutrition 0.000 abstract 1
- 238000006467 substitution reaction Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
- C12P21/00—Preparation of peptides or proteins
- C12P21/06—Preparation of peptides or proteins produced by the hydrolysis of a peptide bond, e.g. hydrolysate products
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/585—Calcitonins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/575—Hormones
- C07K14/63—Motilins
-
- C—CHEMISTRY; METALLURGY
- C07—ORGANIC CHEMISTRY
- C07K—PEPTIDES
- C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
- C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
- C07K14/665—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans derived from pro-opiomelanocortin, pro-enkephalin or pro-dynorphin
- C07K14/695—Corticotropin [ACTH]
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/48—Hydrolases (3) acting on peptide bonds (3.4)
- C12N9/50—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25)
- C12N9/52—Proteinases, e.g. Endopeptidases (3.4.21-3.4.25) derived from bacteria or Archaea
Landscapes
- Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Organic Chemistry (AREA)
- Zoology (AREA)
- Genetics & Genomics (AREA)
- Molecular Biology (AREA)
- General Health & Medical Sciences (AREA)
- Biochemistry (AREA)
- Engineering & Computer Science (AREA)
- Wood Science & Technology (AREA)
- Medicinal Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Biophysics (AREA)
- Toxicology (AREA)
- Gastroenterology & Hepatology (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Microbiology (AREA)
- Endocrinology (AREA)
- Biomedical Technology (AREA)
- Chemical Kinetics & Catalysis (AREA)
- General Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Physics & Mathematics (AREA)
- Peptides Or Proteins (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
Abstract
"MéTODO DE CLIVAGEM DE POLIPEPTìDEO USANDO VARIANTE DE PROTEASE OmpT". A presente invenção refere-se a um método de clivagem de polipeptídeo caracterizado pelo fato de que arginina ou lisina está na posição P1 de um sítio de clivagem desejado em um polipeptídeo, um outro aminoácido que não ácido aspártico, ácido glutâmico ou prolina está na posição P1<39>, um aminoácido básico simples ou dois ou três aminoácidos básicos consecutivos estão localizados em qualquer local na seqüência de aminoácidos da posição P10 para a posição P3 ou da posição P3<39> para a posição P5<39>(contanto que um aminoácido básico simples não esteja localizado na posição P6 ou P4) e protease OmpT ou sua enzima variante tendo uma substituição no 97<198> aminoácido a partir do N-término é usada para clivar o sítio de clivagem desejado no polipeptídeo.
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| JP2003342183 | 2003-09-30 | ||
| PCT/JP2004/014704 WO2005030956A1 (ja) | 2003-09-30 | 2004-09-29 | OmpTプロテアーゼ変異体を用いたポリペプチドの切断方法 |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0414611A true BRPI0414611A (pt) | 2006-11-07 |
Family
ID=34386241
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0414611-5A BRPI0414611A (pt) | 2003-09-30 | 2004-09-29 | método de clivagem de polipeptìdeo usando variante de protease ompt |
Country Status (12)
| Country | Link |
|---|---|
| US (1) | US9657323B2 (pt) |
| EP (1) | EP1674567B1 (pt) |
| JP (1) | JP4663524B2 (pt) |
| KR (1) | KR101099398B1 (pt) |
| CN (1) | CN1860226B (pt) |
| AU (1) | AU2004276687B2 (pt) |
| BR (1) | BRPI0414611A (pt) |
| CA (1) | CA2540446A1 (pt) |
| ES (1) | ES2398463T3 (pt) |
| IL (1) | IL174588A (pt) |
| RU (1) | RU2395582C2 (pt) |
| WO (1) | WO2005030956A1 (pt) |
Families Citing this family (3)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| WO2008038296A2 (en) * | 2006-06-21 | 2008-04-03 | Reliance Life Sciences Pvt Ltd | Recombinant calcitonin fused to interleukin-2 |
| JP6421122B2 (ja) | 2012-10-12 | 2018-11-07 | ストロ バイオファーマ， インコーポレイテッド | 改善された発現のための、細菌抽出物中の選択タンパク質のタンパク質分解不活性化 |
| US12062453B2 (en) | 2018-09-19 | 2024-08-13 | Georgia Tech Research Corporation | Self-titrating bacterial protease-activated prodrug |
Family Cites Families (8)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| GR79124B (pt) | 1982-12-22 | 1984-10-02 | Genentech Inc | |
| US4784949A (en) | 1984-04-19 | 1988-11-15 | Cetus Corporation | Universal dominant selectable marker cassette |
| US5801038A (en) * | 1984-05-29 | 1998-09-01 | Genencor International Inc. | Modified subtilisins having amino acid alterations |
| US5077204A (en) | 1984-06-21 | 1991-12-31 | Chiron Corporation | Yeast endopeptidase for basic amino-acid site cleavage, preparation and use |
| US5013653A (en) | 1987-03-20 | 1991-05-07 | Creative Biomolecules, Inc. | Product and process for introduction of a hinge region into a fusion protein to facilitate cleavage |
| JPH07108232B2 (ja) * | 1990-10-09 | 1995-11-22 | エム・ディ・リサーチ株式会社 | ペプチド又は蛋白質の製造方法 |
| ATE253639T1 (de) | 1991-08-19 | 2003-11-15 | Daiichi Suntory Pharma Co Ltd | Verfahren zur herstellung von peptiden |
| KR100916169B1 (ko) | 1999-03-04 | 2009-09-08 | 아스비오파마 가부시키가이샤 | ＯｍｐＴ 프로테아제에 의한 절단 제어 방법 |
-
2004
- 2004-09-29 KR KR1020067005984A patent/KR101099398B1/ko not_active Expired - Fee Related
- 2004-09-29 CA CA002540446A patent/CA2540446A1/en not_active Abandoned
- 2004-09-29 BR BRPI0414611-5A patent/BRPI0414611A/pt not_active Application Discontinuation
- 2004-09-29 EP EP04773628A patent/EP1674567B1/en not_active Expired - Lifetime
- 2004-09-29 RU RU2006114756/13A patent/RU2395582C2/ru not_active IP Right Cessation
- 2004-09-29 AU AU2004276687A patent/AU2004276687B2/en not_active Ceased
- 2004-09-29 WO PCT/JP2004/014704 patent/WO2005030956A1/ja not_active Ceased
- 2004-09-29 JP JP2005514321A patent/JP4663524B2/ja not_active Expired - Fee Related
- 2004-09-29 US US10/573,821 patent/US9657323B2/en not_active Expired - Fee Related
- 2004-09-29 CN CN2004800285250A patent/CN1860226B/zh not_active Expired - Fee Related
- 2004-09-29 ES ES04773628T patent/ES2398463T3/es not_active Expired - Lifetime
-
2006
- 2006-03-27 IL IL174588A patent/IL174588A/en not_active IP Right Cessation
Also Published As
| Publication number | Publication date |
|---|---|
| RU2395582C2 (ru) | 2010-07-27 |
| EP1674567A4 (en) | 2008-01-09 |
| KR20060089724A (ko) | 2006-08-09 |
| AU2004276687A1 (en) | 2005-04-07 |
| CN1860226A (zh) | 2006-11-08 |
| EP1674567A1 (en) | 2006-06-28 |
| RU2006114756A (ru) | 2007-12-20 |
| IL174588A (en) | 2011-07-31 |
| CA2540446A1 (en) | 2005-04-07 |
| EP1674567B1 (en) | 2013-01-16 |
| IL174588A0 (en) | 2006-08-20 |
| ES2398463T3 (es) | 2013-03-19 |
| KR101099398B1 (ko) | 2011-12-27 |
| CN1860226B (zh) | 2013-09-11 |
| US20070077617A1 (en) | 2007-04-05 |
| JPWO2005030956A1 (ja) | 2006-12-07 |
| JP4663524B2 (ja) | 2011-04-06 |
| US9657323B2 (en) | 2017-05-23 |
| WO2005030956A1 (ja) | 2005-04-07 |
| AU2004276687B2 (en) | 2011-07-14 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B11E | Dismissal acc. art. 34 of ipl - requirements for examination incomplete | ||
| B11T | Dismissal of application maintained [chapter 11.20 patent gazette] |