BRPI0415362A - molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta - Google Patents

molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta

Info

Publication number
BRPI0415362A
BRPI0415362A BRPI0415362-6A BRPI0415362A BRPI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A
Authority
BR
Brazil
Prior art keywords
plant
nucleic acid
nicotine
acid molecule
levels
Prior art date
Application number
BRPI0415362-6A
Other languages
English (en)
Inventor
Dongmei Xu
Original Assignee
Us Smokeless Tobacco Co
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from US10/686,947 external-priority patent/US20040162420A1/en
Priority claimed from US10/943,507 external-priority patent/US7855318B2/en
Application filed by Us Smokeless Tobacco Co filed Critical Us Smokeless Tobacco Co
Publication of BRPI0415362A publication Critical patent/BRPI0415362A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/0004—Oxidoreductases (1.)
    • C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
    • C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Zoology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Cell Biology (AREA)
  • Nutrition Science (AREA)
  • Medicinal Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Cigarettes, Filters, And Manufacturing Of Filters (AREA)

Abstract

"MOLéCULA DE áCIDO NUCLEICO ISOLADA A PARTIR DE NICOTIANA, PROTEìNA ISOLADA DE NICOTIANA, PLANTA TRANSGêNICA, MéTODOS DE PRODUçãO DE UMA PLANTA TRANSGêNICA, DE SELEçãO DE UMA PLANTA CONTENDO UMA MOLéCULA DE áCIDO NUCLEICO, E DE AUMENTO OU DIMINUIçãO DOS NìVEIS DE NORNICOTINA EM UMA PLANTA, PRODUTO E FOLHA DE TABACO TENDO QUANTIDADES REDUZIDAS DE NìVEIS DE NORNICOTINA, E, MéTODO DE ISOLAMENTO DE UM GENE DE UMA PLANTA". A presente invenção refere-se a enzimas P450 e seqüências de ácido nucleico codificando as enzimas p450 em Nicotiana, e métodos de usar estas enzimas e seqüências de ácido nucleico para alterar os fenótipos de plantas.
BRPI0415362-6A 2003-10-16 2004-10-15 molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta BRPI0415362A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US10/686,947 US20040162420A1 (en) 2002-10-16 2003-10-16 Cloning of cytochrome p450 genes from nicotiana
US56623504P 2004-04-29 2004-04-29
US10/943,507 US7855318B2 (en) 2001-11-13 2004-09-17 Cloning of cytochrome P450 genes from Nicotiana
PCT/US2004/034218 WO2005038018A2 (en) 2003-10-16 2004-10-15 Cloning of cytochrome p450 genes from nicotiana

Publications (1)

Publication Number Publication Date
BRPI0415362A true BRPI0415362A (pt) 2006-12-12

Family

ID=34468386

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0415362-6A BRPI0415362A (pt) 2003-10-16 2004-10-15 molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta

Country Status (7)

Country Link
EP (1) EP1694832B1 (pt)
JP (1) JP4519137B2 (pt)
CN (1) CN102703477A (pt)
AU (1) AU2004282588B2 (pt)
BR (1) BRPI0415362A (pt)
PL (1) PL1694832T3 (pt)
WO (1) WO2005038018A2 (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7812227B2 (en) 2001-11-13 2010-10-12 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
US10266836B2 (en) 2001-11-13 2019-04-23 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
US7700834B2 (en) 2001-11-13 2010-04-20 U.S. Smokless Tobacco Company Nicotiana nucleic acid molecules and uses thereof
US7700851B2 (en) 2001-11-13 2010-04-20 U.S. Smokeless Tobacco Company Tobacco nicotine demethylase genomic clone and uses thereof
US8592663B2 (en) 2001-11-13 2013-11-26 U.S. Smokeless Tobacco Company Llc Tobacco nicotine demethylase genomic clone and uses thereof
EP2330191A1 (en) 2003-10-16 2011-06-08 U.S. Smokeless Tobacco Company Cloning of cytochrome P450 genes from nicotiana
US8586837B2 (en) 2004-04-29 2013-11-19 U.S. Smokeless Tobacco Company Llc Nicotiana nucleic acid molecules and uses thereof
JP5106103B2 (ja) * 2004-04-29 2012-12-26 ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー タバコニコチンデメチラーゼゲノムクローンおよびその用途
US20070199097A1 (en) * 2004-09-03 2007-08-23 U.S. Smokeless Tobacco Company Tobacco plants having a mutation in a nicotine demethylase gene
WO2006091194A1 (en) 2005-02-23 2006-08-31 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes
US11332753B2 (en) 2006-12-15 2022-05-17 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8319011B2 (en) 2006-12-15 2012-11-27 U.S. Smokeless Tobacco Company Llc Tobacco plants having reduced nicotine demethylase activity
US8124851B2 (en) 2007-11-12 2012-02-28 North Carolina State University Alteration of tobacco alkaloid content through modification of specific cytochrome P450 genes
CN102858983B (zh) 2010-01-15 2015-08-12 北卡罗来纳州立大学 最小化烟草中去甲烟碱合成的组合物和方法
WO2012118779A1 (en) 2011-02-28 2012-09-07 North Carolina State University Tobacco inbred plants ncbex1f, ncbex1ms, and nc ex90
KR20140034160A (ko) 2011-02-28 2014-03-19 알트리아 클라이언트 서비시스 인코포레이티드 담배 근친교배 식물체 albex1f 및 albex1ms
EP2943059A1 (en) 2013-01-11 2015-11-18 North Carolina State University Tobacco inbred plants k326 src, cms k326 src, k346 src, cms k346 src, nc1562-1 src, nctg-61 src, cms nctg-61 src and hybrid nc196 src
WO2014137802A1 (en) 2013-03-05 2014-09-12 North Carolina State University Tobacco inbred and hybrid plants and uses thereof
WO2015134423A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
EP3113604A1 (en) 2014-03-03 2017-01-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
WO2015134438A1 (en) 2014-03-03 2015-09-11 North Carolina State University Tobacco inbred and hybrid plants and tobacco products made thereof
KR20170052647A (ko) * 2014-09-09 2017-05-12 에볼바 에스아 재조합 숙주에서 스테비올 글리코시드의 생성

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20040162420A1 (en) * 2002-10-16 2004-08-19 U.S. Smokeless Tobacco Company Cloning of cytochrome p450 genes from nicotiana
JP2005531297A (ja) * 2002-03-12 2005-10-20 ユーエス スモークレス タバコ カンパニー タバコ由来シトクロムｐ４５０遺伝子のクローニング
JP2006519590A (ja) * 2002-10-16 2006-08-31 ユーエス スモークレス タバコ カンパニー タバコ属由来のシトクロムｐ４５０遺伝子のクローニング
EP1675957B1 (en) * 2003-10-16 2011-12-07 U.S. Smokeless Tobacco Company LLC Cloning of cytochrome p450 genes from nicotiana

Also Published As

Publication number Publication date
CN102703477A (zh) 2012-10-03
WO2005038018A2 (en) 2005-04-28
AU2004282588B2 (en) 2010-01-28
WO2005038018A8 (en) 2005-09-22
HK1104575A1 (en) 2008-01-18
AU2004282588A1 (en) 2005-04-28
EP1694832B1 (en) 2012-04-18
EP1694832A2 (en) 2006-08-30
JP2007512003A (ja) 2007-05-17
PL1694832T3 (pl) 2012-09-28
JP4519137B2 (ja) 2010-08-04

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Legal Events

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B25D Requested change of name of applicant approved

Owner name: U.S. SMOKELESS TOBACCO COMPANY LLC (US)

Free format text: NOME ALTERADO DE: U.S. SMOKELESS TOBACCO COMPANY

B15G Others concerning applications: unknown petition

Free format text: DESCONHECIDA A ALTERACAO DE SEDE, REQUERIDA ATRAVES DA PETICAO NO 020120016179/RJ DE 27/02/2012, POR AUSENCIA DE FUNDAMENTACAO LEGAL, UMA VEZ QUE O ENDERECO REQUERIDO E O DO DEPOSITANTE, E O MESMO.

B150 Others concerning applications: publication cancelled

Free format text: ANULADO O DESCONHECIMENTO DA PETICAO, CODIGO DO DESPACHO (15.7), PUBLICADO NA RPI 2172 DE 21/08/2012, POR TER SIDO INDEVIDO.

B25G Requested change of headquarter approved

Owner name: U.S. SMOKELESS TOBACCO COMPANY LLC (US)

Free format text: ENDERECO ALTERADO CONFORME SOLICITADO NA PETICAO NO 020120016179/RJ DE 27/02/2012.

B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 9/02 , C12N 15/82 , A01H 5/12

Ipc: C12N 15/82 (2006.01), A01H 5/12 (2006.01), C12N 9/

B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application according art. 36 industrial patent law
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements