BRPI0415362A - molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta - Google Patents
molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma plantaInfo
- Publication number
- BRPI0415362A BRPI0415362A BRPI0415362-6A BRPI0415362A BRPI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A BR PI0415362 A BRPI0415362 A BR PI0415362A
- Authority
- BR
- Brazil
- Prior art keywords
- plant
- nucleic acid
- nicotine
- acid molecule
- levels
- Prior art date
Links
- 241000196324 Embryophyta Species 0.000 title abstract 8
- 150000007523 nucleic acids Chemical class 0.000 title abstract 5
- 238000000034 method Methods 0.000 title abstract 4
- 108090000623 proteins and genes Proteins 0.000 title abstract 4
- 230000009261 transgenic effect Effects 0.000 title abstract 4
- MYKUKUCHPMASKF-VIFPVBQESA-N (S)-nornicotine Chemical compound C1CCN[C@@H]1C1=CC=CN=C1 MYKUKUCHPMASKF-VIFPVBQESA-N 0.000 title abstract 3
- 102000039446 nucleic acids Human genes 0.000 title abstract 3
- 108020004707 nucleic acids Proteins 0.000 title abstract 3
- MYKUKUCHPMASKF-UHFFFAOYSA-N Nornicotine Natural products C1CCNC1C1=CC=CN=C1 MYKUKUCHPMASKF-UHFFFAOYSA-N 0.000 title abstract 2
- 102000004169 proteins and genes Human genes 0.000 title abstract 2
- 235000019505 tobacco product Nutrition 0.000 title abstract 2
- 230000003247 decreasing effect Effects 0.000 title 1
- 241000208125 Nicotiana Species 0.000 abstract 3
- 102000002004 Cytochrome P-450 Enzyme System Human genes 0.000 abstract 2
- 108010015742 Cytochrome P-450 Enzyme System Proteins 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 1
- 108090000790 Enzymes Proteins 0.000 abstract 1
- 244000061176 Nicotiana tabacum Species 0.000 abstract 1
- 235000002637 Nicotiana tabacum Nutrition 0.000 abstract 1
- 238000002955 isolation Methods 0.000 abstract 1
- 238000004519 manufacturing process Methods 0.000 abstract 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/0004—Oxidoreductases (1.)
- C12N9/0071—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14)
- C12N9/0077—Oxidoreductases (1.) acting on paired donors with incorporation of molecular oxygen (1.14) with a reduced iron-sulfur protein as one donor (1.14.15)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
- C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
Landscapes
- Health & Medical Sciences (AREA)
- Life Sciences & Earth Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Chemical & Material Sciences (AREA)
- Zoology (AREA)
- Biotechnology (AREA)
- Organic Chemistry (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Wood Science & Technology (AREA)
- Molecular Biology (AREA)
- General Engineering & Computer Science (AREA)
- Biomedical Technology (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- Microbiology (AREA)
- Nutrition Science (AREA)
- Physics & Mathematics (AREA)
- Cell Biology (AREA)
- Biophysics (AREA)
- Plant Pathology (AREA)
- Medicinal Chemistry (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Enzymes And Modification Thereof (AREA)
- Cigarettes, Filters, And Manufacturing Of Filters (AREA)
Abstract
"MOLéCULA DE áCIDO NUCLEICO ISOLADA A PARTIR DE NICOTIANA, PROTEìNA ISOLADA DE NICOTIANA, PLANTA TRANSGêNICA, MéTODOS DE PRODUçãO DE UMA PLANTA TRANSGêNICA, DE SELEçãO DE UMA PLANTA CONTENDO UMA MOLéCULA DE áCIDO NUCLEICO, E DE AUMENTO OU DIMINUIçãO DOS NìVEIS DE NORNICOTINA EM UMA PLANTA, PRODUTO E FOLHA DE TABACO TENDO QUANTIDADES REDUZIDAS DE NìVEIS DE NORNICOTINA, E, MéTODO DE ISOLAMENTO DE UM GENE DE UMA PLANTA". A presente invenção refere-se a enzimas P450 e seqüências de ácido nucleico codificando as enzimas p450 em Nicotiana, e métodos de usar estas enzimas e seqüências de ácido nucleico para alterar os fenótipos de plantas.
Applications Claiming Priority (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US10/686,947 US20040162420A1 (en) | 2002-10-16 | 2003-10-16 | Cloning of cytochrome p450 genes from nicotiana |
| US56623504P | 2004-04-29 | 2004-04-29 | |
| US10/943,507 US7855318B2 (en) | 2001-11-13 | 2004-09-17 | Cloning of cytochrome P450 genes from Nicotiana |
| PCT/US2004/034218 WO2005038018A2 (en) | 2003-10-16 | 2004-10-15 | Cloning of cytochrome p450 genes from nicotiana |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0415362A true BRPI0415362A (pt) | 2006-12-12 |
Family
ID=34468386
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0415362-6A BRPI0415362A (pt) | 2003-10-16 | 2004-10-15 | molécula de ácido nucleico isolada a partir de nicotina, proteìna isolada de nicotina, planta transgênica, métodos de produção de uma planta transgênica, de seleção de uma planta contendo uma molécula de ácido nucleico, e de aumento ou diminuição dos nìveis de nornicotina em uma planta, produto e folha de tabaco tendo quantidades reduzidas de nìveis de nornicotina, e, método de isolamento de um gene de uma planta |
Country Status (7)
| Country | Link |
|---|---|
| EP (1) | EP1694832B1 (pt) |
| JP (1) | JP4519137B2 (pt) |
| CN (1) | CN102703477A (pt) |
| AU (1) | AU2004282588B2 (pt) |
| BR (1) | BRPI0415362A (pt) |
| PL (1) | PL1694832T3 (pt) |
| WO (1) | WO2005038018A2 (pt) |
Families Citing this family (22)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US7812227B2 (en) | 2001-11-13 | 2010-10-12 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| US7700834B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokless Tobacco Company | Nicotiana nucleic acid molecules and uses thereof |
| US7700851B2 (en) | 2001-11-13 | 2010-04-20 | U.S. Smokeless Tobacco Company | Tobacco nicotine demethylase genomic clone and uses thereof |
| US8592663B2 (en) | 2001-11-13 | 2013-11-26 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| US10266836B2 (en) | 2001-11-13 | 2019-04-23 | U.S. Smokeless Tobacco Company Llc | Tobacco nicotine demethylase genomic clone and uses thereof |
| CN1926235B (zh) | 2003-10-16 | 2012-07-18 | 美国无烟烟草有限责任公司 | 从烟草克隆细胞色素p450基因 |
| JP5106103B2 (ja) * | 2004-04-29 | 2012-12-26 | ユーエス スモークレス タバコ カンパニー リミテッド ライアビリティ カンパニー | タバコニコチンデメチラーゼゲノムクローンおよびその用途 |
| US8586837B2 (en) | 2004-04-29 | 2013-11-19 | U.S. Smokeless Tobacco Company Llc | Nicotiana nucleic acid molecules and uses thereof |
| US20070199097A1 (en) * | 2004-09-03 | 2007-08-23 | U.S. Smokeless Tobacco Company | Tobacco plants having a mutation in a nicotine demethylase gene |
| ATE530656T1 (de) | 2005-02-23 | 2011-11-15 | Univ North Carolina State | Veränderung des alkaloidgehaltes in tabak durch modifikation spezifischer cytochrome p450 gene. |
| US8319011B2 (en) | 2006-12-15 | 2012-11-27 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| US11332753B2 (en) | 2006-12-15 | 2022-05-17 | U.S. Smokeless Tobacco Company Llc | Tobacco plants having reduced nicotine demethylase activity |
| WO2009064771A2 (en) | 2007-11-12 | 2009-05-22 | North Carolina State University | Alteration of tobacco alkaloid content through modification of specific cytochrome p450 genes |
| PH12012501393B1 (en) | 2010-01-15 | 2017-10-25 | Univ North Carolina State | Compositions and methods for minimizing nornicotine synthesis in tobacco |
| US9096864B2 (en) | 2011-02-28 | 2015-08-04 | North Carolina State University | Tobacco inbred plants NCBEX1F, NCBEX1MS, and NC EX90 |
| EP2681321A1 (en) | 2011-02-28 | 2014-01-08 | Altria Client Services Inc. | Tobacco inbred plants albex1f and albex1ms |
| US9603335B2 (en) | 2013-01-11 | 2017-03-28 | North Carolina State University | Tobacco inbred plants K326 SRC, CMS K326 SRC, K346 SRC, CMS K346 SRC, NC1562-1 SRC, NCTG-61 SRC, CMS NCTG-61 SRC and hybrid NC196 SRC |
| US9560830B2 (en) | 2013-03-05 | 2017-02-07 | North Carolina State University | Tobacco inbred and hybrid plants and uses thereof |
| EP3113604A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| EP3113603A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| EP3113602A1 (en) | 2014-03-03 | 2017-01-11 | North Carolina State University | Tobacco inbred and hybrid plants and tobacco products made thereof |
| AU2015314251A1 (en) * | 2014-09-09 | 2017-03-16 | Evolva Sa | Production of steviol glycosides in recombinant hosts |
Family Cites Families (4)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20040162420A1 (en) * | 2002-10-16 | 2004-08-19 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| CA2477452A1 (en) * | 2002-03-12 | 2003-09-25 | U.S. Smokeless Tobacco Company | Cloning of cytochrome p450 genes from nicotiana |
| EP2336305A3 (en) * | 2002-10-16 | 2011-10-05 | U.S. Smokeless Tobacco Company LLC | Cloning of cytochrome P450 genes from Nicotiana |
| PL1675957T3 (pl) * | 2003-10-16 | 2012-06-29 | U S Smokeless Tobacco Company Llc | Klonowanie genów cytochromu p450 z Nicotiana |
-
2004
- 2004-10-15 EP EP04795391A patent/EP1694832B1/en not_active Expired - Lifetime
- 2004-10-15 CN CN2012101717953A patent/CN102703477A/zh active Pending
- 2004-10-15 WO PCT/US2004/034218 patent/WO2005038018A2/en not_active Ceased
- 2004-10-15 PL PL04795391T patent/PL1694832T3/pl unknown
- 2004-10-15 AU AU2004282588A patent/AU2004282588B2/en not_active Expired
- 2004-10-15 BR BRPI0415362-6A patent/BRPI0415362A/pt not_active Application Discontinuation
- 2004-10-15 JP JP2006535378A patent/JP4519137B2/ja not_active Expired - Fee Related
Also Published As
| Publication number | Publication date |
|---|---|
| PL1694832T3 (pl) | 2012-09-28 |
| WO2005038018A2 (en) | 2005-04-28 |
| AU2004282588A1 (en) | 2005-04-28 |
| CN102703477A (zh) | 2012-10-03 |
| JP4519137B2 (ja) | 2010-08-04 |
| HK1104575A1 (en) | 2008-01-18 |
| JP2007512003A (ja) | 2007-05-17 |
| EP1694832A2 (en) | 2006-08-30 |
| WO2005038018A8 (en) | 2005-09-22 |
| EP1694832B1 (en) | 2012-04-18 |
| AU2004282588B2 (en) | 2010-01-28 |
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Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B25D | Requested change of name of applicant approved |
Owner name: U.S. SMOKELESS TOBACCO COMPANY LLC (US) Free format text: NOME ALTERADO DE: U.S. SMOKELESS TOBACCO COMPANY |
|
| B15G | Others concerning applications: unknown petition |
Free format text: DESCONHECIDA A ALTERACAO DE SEDE, REQUERIDA ATRAVES DA PETICAO NO 020120016179/RJ DE 27/02/2012, POR AUSENCIA DE FUNDAMENTACAO LEGAL, UMA VEZ QUE O ENDERECO REQUERIDO E O DO DEPOSITANTE, E O MESMO. |
|
| B150 | Others concerning applications: publication cancelled |
Free format text: ANULADO O DESCONHECIMENTO DA PETICAO, CODIGO DO DESPACHO (15.7), PUBLICADO NA RPI 2172 DE 21/08/2012, POR TER SIDO INDEVIDO. |
|
| B25G | Requested change of headquarter approved |
Owner name: U.S. SMOKELESS TOBACCO COMPANY LLC (US) Free format text: ENDERECO ALTERADO CONFORME SOLICITADO NA PETICAO NO 020120016179/RJ DE 27/02/2012. |
|
| B06F | Objections, documents and/or translations needed after an examination request according art. 34 industrial property law | ||
| B06F | Objections, documents and/or translations needed after an examination request according art. 34 industrial property law | ||
| B15K | Others concerning applications: alteration of classification |
Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 9/02 , C12N 15/82 , A01H 5/12 Ipc: C12N 15/82 (2006.01), A01H 5/12 (2006.01), C12N 9/ |
|
| B06A | Notification to applicant to reply to the report for non-patentability or inadequacy of the application according art. 36 industrial patent law | ||
| B11B | Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements |