BRPI0415435A - método e kit para amplificação baseada em iniciador de ácidos nucleicos - Google Patents

método e kit para amplificação baseada em iniciador de ácidos nucleicos

Info

Publication number
BRPI0415435A
BRPI0415435A BRPI0415435-5A BRPI0415435A BRPI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A
Authority
BR
Brazil
Prior art keywords
amplification
nucleic acid
target
tempcr
pathogens
Prior art date
Application number
BRPI0415435-5A
Other languages
English (en)
Inventor
Jian Han
Original Assignee
Genaco Biomedical Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genaco Biomedical Products Inc filed Critical Genaco Biomedical Products Inc
Publication of BRPI0415435A publication Critical patent/BRPI0415435A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/102—Multiple non-interacting labels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODO E KIT PARA AMPLIFICAçãO BASEADA EM INICIADOR DE áCIDOS NUCLEICOS". Um novo método para diagnóstico ou diagnóstico diferencial de agentes patogênicos e agentes patogênicos secundários é divulgado. O método divulgado utiliza um nova estratégia de amplificação denominada TemPCR para permitir amplificação sensível e específica de seqüências alvo de quaisquer agentes patogênicos e/ou agentes patogênicos secundários cuja seqüência de ácido nucleico seja conhecida. o método TemPCR utiliza pelo menos um grupo de iniciadores de enriquecimento de alvo específicos para o agente patogênico ou para o agente patogênico secundário a ser detectado (presente em uma baixa concentração) e pelo menos um par de iniciadores de amplificação de alvo compartilhado (presentes em altas concentrações) . Pelo menos um par dos citados iniciadores de enriquecimento de alvo compreende uma seqüência de ligação para os iniciadores de amplificação de alvo. Portanto, a utilização do método TemPCR permite a execução de reações de amplificação múltiplas sem a necessidade de otimização empírica dos parâmetros de amplificação múltipla. Métodos para o isolamento de ácido nucleico e para a detecção das seqüências alvo também são divulgadas para utilização com o método TemPCR.
BRPI0415435-5A 2003-10-13 2004-10-13 método e kit para amplificação baseada em iniciador de ácidos nucleicos BRPI0415435A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51076203P 2003-10-13 2003-10-13
PCT/US2004/033818 WO2005038039A2 (en) 2003-10-13 2004-10-13 Method for multiplex primer based amplification of nucleic acids

Publications (1)

Publication Number Publication Date
BRPI0415435A true BRPI0415435A (pt) 2006-12-05

Family

ID=34465146

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0415435-5A BRPI0415435A (pt) 2003-10-13 2004-10-13 método e kit para amplificação baseada em iniciador de ácidos nucleicos

Country Status (10)

Country Link
US (1) US7851148B2 (pt)
EP (1) EP1687437A4 (pt)
JP (1) JP4999460B2 (pt)
KR (1) KR100830623B1 (pt)
CN (1) CN1898395B (pt)
AU (1) AU2004282555B2 (pt)
BR (1) BRPI0415435A (pt)
CA (1) CA2542787C (pt)
NZ (1) NZ546903A (pt)
WO (1) WO2005038039A2 (pt)

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Also Published As

Publication number Publication date
CN1898395B (zh) 2014-02-12
EP1687437A4 (en) 2008-01-02
KR20060088128A (ko) 2006-08-03
JP2007508824A (ja) 2007-04-12
US20070141575A1 (en) 2007-06-21
WO2005038039A2 (en) 2005-04-28
US7851148B2 (en) 2010-12-14
AU2004282555B2 (en) 2009-05-28
CN1898395A (zh) 2007-01-17
NZ546903A (en) 2009-06-26
CA2542787C (en) 2013-10-29
KR100830623B1 (ko) 2008-05-20
AU2004282555A1 (en) 2005-04-28
WO2005038039A3 (en) 2006-09-28
JP4999460B2 (ja) 2012-08-15
EP1687437A2 (en) 2006-08-09
CA2542787A1 (en) 2005-04-28

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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