BRPI0415435A - método e kit para amplificação baseada em iniciador de ácidos nucleicos - Google Patents

método e kit para amplificação baseada em iniciador de ácidos nucleicos

Info

Publication number
BRPI0415435A
BRPI0415435A BRPI0415435-5A BRPI0415435A BRPI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A BR PI0415435 A BRPI0415435 A BR PI0415435A
Authority
BR
Brazil
Prior art keywords
amplification
nucleic acid
target
tempcr
pathogens
Prior art date
Application number
BRPI0415435-5A
Other languages
English (en)
Inventor
Jian Han
Original Assignee
Genaco Biomedical Products Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genaco Biomedical Products Inc filed Critical Genaco Biomedical Products Inc
Publication of BRPI0415435A publication Critical patent/BRPI0415435A/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/155—Modifications characterised by incorporating/generating a new priming site
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2527/00—Reactions demanding special reaction conditions
    • C12Q2527/143—Concentration of primer or probe
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2531/00—Reactions of nucleic acids characterised by
    • C12Q2531/10—Reactions of nucleic acids characterised by the purpose being amplify/increase the copy number of target nucleic acid
    • C12Q2531/113—PCR
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2537/00—Reactions characterised by the reaction format or use of a specific feature
    • C12Q2537/10—Reactions characterised by the reaction format or use of a specific feature the purpose or use of
    • C12Q2537/143—Multiplexing, i.e. use of multiple primers or probes in a single reaction, usually for simultaneously analyse of multiple analysis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2549/00—Reactions characterised by the features used to influence the efficiency or specificity
    • C12Q2549/10—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals
    • C12Q2549/119—Reactions characterised by the features used to influence the efficiency or specificity the purpose being that of reducing false positive or false negative signals using nested primers
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2565/00—Nucleic acid analysis characterised by mode or means of detection
    • C12Q2565/10—Detection mode being characterised by the assay principle
    • C12Q2565/102—Multiple non-interacting labels
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A50/00—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE in human health protection, e.g. against extreme weather
    • Y02A50/30—Against vector-borne diseases, e.g. mosquito-borne, fly-borne, tick-borne or waterborne diseases whose impact is exacerbated by climate change

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

"MéTODO E KIT PARA AMPLIFICAçãO BASEADA EM INICIADOR DE áCIDOS NUCLEICOS". Um novo método para diagnóstico ou diagnóstico diferencial de agentes patogênicos e agentes patogênicos secundários é divulgado. O método divulgado utiliza um nova estratégia de amplificação denominada TemPCR para permitir amplificação sensível e específica de seqüências alvo de quaisquer agentes patogênicos e/ou agentes patogênicos secundários cuja seqüência de ácido nucleico seja conhecida. o método TemPCR utiliza pelo menos um grupo de iniciadores de enriquecimento de alvo específicos para o agente patogênico ou para o agente patogênico secundário a ser detectado (presente em uma baixa concentração) e pelo menos um par de iniciadores de amplificação de alvo compartilhado (presentes em altas concentrações) . Pelo menos um par dos citados iniciadores de enriquecimento de alvo compreende uma seqüência de ligação para os iniciadores de amplificação de alvo. Portanto, a utilização do método TemPCR permite a execução de reações de amplificação múltiplas sem a necessidade de otimização empírica dos parâmetros de amplificação múltipla. Métodos para o isolamento de ácido nucleico e para a detecção das seqüências alvo também são divulgadas para utilização com o método TemPCR.
BRPI0415435-5A 2003-10-13 2004-10-13 método e kit para amplificação baseada em iniciador de ácidos nucleicos BRPI0415435A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US51076203P 2003-10-13 2003-10-13
PCT/US2004/033818 WO2005038039A2 (en) 2003-10-13 2004-10-13 Method for multiplex primer based amplification of nucleic acids

Publications (1)

Publication Number Publication Date
BRPI0415435A true BRPI0415435A (pt) 2006-12-05

Family

ID=34465146

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0415435-5A BRPI0415435A (pt) 2003-10-13 2004-10-13 método e kit para amplificação baseada em iniciador de ácidos nucleicos

Country Status (10)

Country Link
US (1) US7851148B2 (pt)
EP (1) EP1687437A4 (pt)
JP (1) JP4999460B2 (pt)
KR (1) KR100830623B1 (pt)
CN (1) CN1898395B (pt)
AU (1) AU2004282555B2 (pt)
BR (1) BRPI0415435A (pt)
CA (1) CA2542787C (pt)
NZ (1) NZ546903A (pt)
WO (1) WO2005038039A2 (pt)

Families Citing this family (36)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006244460B2 (en) * 2005-05-06 2010-04-08 Gen-Probe Incorporated Compositions and assays to detect influenza virus A and B nucleic acids
KR20080047355A (ko) * 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
US20080053911A1 (en) * 2006-08-30 2008-03-06 Qiagen Gmbh Separation of an organic phase from a mixture comprising organic and aqueous phases by solid phase systems
US8198027B2 (en) 2006-12-21 2012-06-12 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
GB0703996D0 (en) * 2007-03-01 2007-04-11 Oxitec Ltd Nucleic acid detection
JP5730568B2 (ja) * 2007-06-01 2015-06-10 モノクアント・ピーティーワイ・リミテッド Ｄｎａ切断点の分析のための方法
CA2700233A1 (en) * 2007-10-22 2009-04-30 Monoquant Pty Ltd. A method of dna amplification
CN104928335A (zh) * 2008-04-03 2015-09-23 哈森阿尔法生物技术研究院 用于扩增多个靶标的扩增子拯救多重聚合酶链式反应
US9012148B2 (en) * 2008-04-16 2015-04-21 Jian Han Method for evaluating and comparing immunorepertoires
WO2010027870A2 (en) * 2008-08-26 2010-03-11 Fluidigm Corporation Assay methods for increased throughput of samples and/or targets
EP2789694A1 (en) 2009-04-02 2014-10-15 Fluidigm Corporation Microfluidic device with reaction product recovery system
EP2449132B1 (en) 2009-07-01 2015-05-13 Gen-Probe Incorporated Methods and compositions for nucleic acid amplification
US9416419B2 (en) 2009-11-16 2016-08-16 Michael E. Hogan Methods for PCR and HLA typing using unpurified samples
US8771951B2 (en) * 2009-11-16 2014-07-08 Genomics Usa, Inc. Methods for PCR and HLA typing using raw blood
KR101287431B1 (ko) * 2010-05-07 2013-07-19 (주)진매트릭스 표적 유전자의 다양한 변이가 존재하는 유전자 영역을 증폭하기 위한 프라이머 조성물, 이를 이용한 표적 유전자 증폭 방법 및 이를 포함하는 ｐｃｒ 증폭 키트 그리고 이를 이용한 표적 유전자의 유전자형 분석방법
CA2799200A1 (en) * 2010-05-19 2011-11-24 Qiagen Gaithersburg, Inc. Methods and compositions for sequence-specific purification and multiplex analysis of nucleic acids
CN102286612B (zh) * 2010-06-18 2014-10-22 中国科学院上海生命科学研究院 一种致病微生物快速检测试剂盒
US9074204B2 (en) 2011-05-20 2015-07-07 Fluidigm Corporation Nucleic acid encoding reactions
KR101483576B1 (ko) * 2011-10-31 2015-01-21 아크레이 가부시키가이샤 유전자 존재량의 측정 방법
US9115394B2 (en) 2011-12-22 2015-08-25 Roche Molecular Systems, Inc. Methods and reagents for reducing non-specific amplification
US20150045258A1 (en) * 2012-02-14 2015-02-12 Gnubio, Inc. Cascaded addition of target specific universal adapters to nucleic acids
JP2015519900A (ja) 2012-05-21 2015-07-16 フリューダイム・コーポレイション 粒子集団の単粒子解析方法及び単粒子単離方法
US9139870B2 (en) 2012-08-30 2015-09-22 Gen-Probe Incorporated Multiphase nucleic acid amplification
CN104250663B (zh) * 2013-06-27 2017-09-15 北京大学 甲基化CpG岛的高通量测序检测方法
CN103981278B (zh) * 2014-06-06 2015-08-19 黑龙江出入境检验检疫局检验检疫技术中心 利用Tem-PCR技术检测多种致病菌的PCR检测通用引物对
US11117113B2 (en) 2015-12-16 2021-09-14 Fluidigm Corporation High-level multiplex amplification
CN105543410B (zh) * 2015-12-25 2019-06-07 四川农业大学 基于tem-pcr和基因芯片检测猪病毒性疾病的方法
CN106834546A (zh) * 2017-03-20 2017-06-13 杭州迪安医学检验中心有限公司 一种基于熔解曲线法单管同时检测多种呼吸道病毒的引物及其应用
US20180340214A1 (en) * 2017-05-25 2018-11-29 Diatherix Laboratories, Inc. Quantitative multiplex polymerase chain reaction in two reactions
CN111206081B (zh) * 2018-11-21 2023-06-30 思纳福(苏州)生命科技有限公司 核酸检测微球、制备方法、试剂盒及高通量核酸检测方法
CN109609615A (zh) * 2018-12-06 2019-04-12 云南中医学院 一种缺血性脑卒中miRNA标记物的检测方法
CN109609693A (zh) * 2018-12-26 2019-04-12 山东艾克韦生物技术有限公司 一种同时检测多种肠道病毒的方法
CN109913592A (zh) * 2019-04-30 2019-06-21 广西大学 ENTV-2的SYBR GreenⅠ荧光定量PCR检测引物对及诊断试剂盒
CN112593011A (zh) * 2020-12-25 2021-04-02 中山大学 一组检测柯萨奇病毒b组的引物和探针
CN113637799B (zh) * 2021-08-12 2023-09-29 北京农业生物技术研究中心 一种菊花b病毒的rpa检测方法
WO2025252784A1 (de) 2024-06-06 2025-12-11 Robert Bosch Gmbh Verfahren, primer-set und kit zum gleichzeitigen amplifizieren von einer mehr-zahl von ziel-nukleinsäuresequenzen in einer probe

Family Cites Families (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
US5194300A (en) * 1987-07-15 1993-03-16 Cheung Sau W Methods of making fluorescent microspheres
JPH0491790A (ja) 1990-08-02 1992-03-25 Shionogi & Co Ltd ２段階ｐｃｒ法
US5341809A (en) * 1990-08-31 1994-08-30 Hitachi, Ltd. Ultrasonic flowmeter
EP0519338B1 (en) * 1991-06-20 1996-08-28 F. Hoffmann-La Roche Ag Improved methods for nucleic acid amplification
GB2259138B (en) * 1991-08-27 1995-08-23 Ici Plc Minisatellite variant repeat (MVR-PRC) mapping
US5340728A (en) 1992-12-09 1994-08-23 E. I. Du Pont De Nemours And Company Method for amplification of targeted segments of nucleic acid using nested polymerase chain reaction
US5422252A (en) 1993-06-04 1995-06-06 Becton, Dickinson And Company Simultaneous amplification of multiple targets
KR100189229B1 (ko) 1993-07-23 1999-06-01 다니엘 엘. 캐시앙, 헨리 엘. 노르호프 핵산 증폭을 강화하는 방법
US5882856A (en) * 1995-06-07 1999-03-16 Genzyme Corporation Universal primer sequence for multiplex DNA amplification
US5642825A (en) * 1995-08-21 1997-07-01 Superseal Corporation Container closure having peripheral tamper-indicator
GB9609441D0 (en) * 1996-05-04 1996-07-10 Zeneca Ltd Process
ATE229571T1 (de) 1996-10-02 2002-12-15 Us Gov Health & Human Serv Nachweis und identifizierung von nicht-polio enteroviren
AUPQ008799A0 (en) 1999-04-30 1999-05-27 Tillett, Daniel Genome sequencing
US6509157B1 (en) 1999-11-05 2003-01-21 Roche Molecular Systems, Inc 3 blocked nucleic acid amplification primers
US6605451B1 (en) 2000-06-06 2003-08-12 Xtrana, Inc. Methods and devices for multiplexing amplification reactions
US6566067B2 (en) 2001-02-14 2003-05-20 Synthegen Systems, Inc. High fidelity PCR cloning
AU2002305941A1 (en) * 2001-03-01 2002-09-19 The Johns Hopkins University Quantitative assay for the simultaneous detection and speciation of bacterial infections
AU2002305436A1 (en) 2001-05-09 2002-11-18 Virginia Commonwealth University Multiple sequencible and ligatible structures for genomic analysis
EP1421200B1 (en) * 2001-08-23 2006-12-13 Merck & Co., Inc. Fluorescent multiplex hpv pcr assays using multiple fluorophores
WO2003020983A1 (en) * 2001-08-30 2003-03-13 Virginia Commonwealth University Allele specific pcr for genotyping
US7153656B2 (en) 2001-09-11 2006-12-26 Los Alamos National Security, Llc Nucleic acid sequence detection using multiplexed oligonucleotide PCR
CA2467460A1 (en) 2001-11-19 2003-05-30 Parallele Bioscience, Inc. Multiplex oligonucleotide addition and target amplification
WO2003060159A2 (en) 2002-01-15 2003-07-24 Matforsk Methods of nucleic acid amplification
EP1474529B1 (en) 2002-02-08 2008-10-22 Olympus Corporation Specific multiplex analysis of nucleic acids

Also Published As

Publication number Publication date
US7851148B2 (en) 2010-12-14
EP1687437A2 (en) 2006-08-09
KR20060088128A (ko) 2006-08-03
JP4999460B2 (ja) 2012-08-15
CA2542787A1 (en) 2005-04-28
AU2004282555A1 (en) 2005-04-28
NZ546903A (en) 2009-06-26
CN1898395A (zh) 2007-01-17
CA2542787C (en) 2013-10-29
KR100830623B1 (ko) 2008-05-20
JP2007508824A (ja) 2007-04-12
CN1898395B (zh) 2014-02-12
WO2005038039A3 (en) 2006-09-28
EP1687437A4 (en) 2008-01-02
US20070141575A1 (en) 2007-06-21
WO2005038039A2 (en) 2005-04-28
AU2004282555B2 (en) 2009-05-28

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B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

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