BRPI0418992A - processo para a purificação de hormÈnio do crescimento humano - Google Patents

processo para a purificação de hormÈnio do crescimento humano

Info

Publication number
BRPI0418992A
BRPI0418992A BRPI0418992-2A BRPI0418992A BRPI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A BR PI0418992 A BRPI0418992 A BR PI0418992A
Authority
BR
Brazil
Prior art keywords
detergent
growth hormone
human growth
elution buffer
buffer
Prior art date
Application number
BRPI0418992-2A
Other languages
English (en)
Inventor
Ahmed Monsur Borbhuiya
Yada Madhava Rao
Laxmi Srinivas Rao
Dipanwita Maiti
Prabhakar Milind Niphadkar
Shrikant Mishra
Original Assignee
Usv Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Usv Ltd filed Critical Usv Ltd
Publication of BRPI0418992A publication Critical patent/BRPI0418992A/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K14/00Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575Hormones
    • C07K14/61Growth hormone [GH], i.e. somatotropin
    • CCHEMISTRY; METALLURGY
    • C07ORGANIC CHEMISTRY
    • C07KPEPTIDES
    • C07K1/00General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14Extraction; Separation; Purification
    • C07K1/16Extraction; Separation; Purification by chromatography
    • C07K1/20Partition-, reverse-phase or hydrophobic interaction chromatography

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Endocrinology (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Biophysics (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Zoology (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Peptides Or Proteins (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROCESSO PARA A PURIFICAçãO DE HORMÈNIO DO CRESCIMENTO HUMANO. A presente invenção refere-se a um processo comercial para a purificação de hormónio do crescimento humano obtido por uma técnica de DNA recombinante usando cromatografia interativa hidrofóbica, eficiente para separar moléculas encurtadas de hormónio do crescimento humano, presentes como uma impureza que surge devido a posições de corte presentes na megamolécula de hormónio do crescimento humano, da molécula de hormónio do crescimento humano-alvo, usando uma coluna fenila, uma mistura de tampões aquosos e solventes orgânicos como eluente, seguido por dessalinização, liofilização, e a invenção também refere-se ao processo em que a eluição de moléculas encurtadas de hormónio do crescimento humano precede a eluição do hormónio do crescimento humano-alvo, para se obter hGH de pureza > 99,5%.
BRPI0418992-2A 2004-11-09 2004-11-09 processo para a purificação de hormÈnio do crescimento humano BRPI0418992A (pt)

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
PCT/IN2004/000346 WO2006051554A1 (en) 2004-11-09 2004-11-09 A novel process for purification of human growth harmone

Publications (1)

Publication Number Publication Date
BRPI0418992A true BRPI0418992A (pt) 2007-12-11

Family

ID=34964528

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0418992-2A BRPI0418992A (pt) 2004-11-09 2004-11-09 processo para a purificação de hormÈnio do crescimento humano

Country Status (10)

Country Link
US (1) US20080167452A1 (pt)
EP (1) EP1809664B1 (pt)
CN (1) CN101061137A (pt)
AT (1) ATE395360T1 (pt)
AU (1) AU2004324756A1 (pt)
BR (1) BRPI0418992A (pt)
CA (1) CA2565209A1 (pt)
DE (1) DE602004013847D1 (pt)
EA (1) EA200602097A1 (pt)
WO (1) WO2006051554A1 (pt)

Families Citing this family (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8080391B2 (en) * 2004-12-22 2011-12-20 Ambrx, Inc. Process of producing non-naturally encoded amino acid containing high conjugated to a water soluble polymer
WO2010096394A2 (en) 2009-02-17 2010-08-26 Redwood Biosciences, Inc. Aldehyde-tagged protein-based drug carriers and methods of use
AU2011343813B2 (en) 2010-12-15 2015-05-21 Takeda Pharmaceutical Company Limited Eluate collection using conductivity gradient
US9540438B2 (en) 2011-01-14 2017-01-10 Redwood Bioscience, Inc. Aldehyde-tagged immunoglobulin polypeptides and methods of use thereof
US8679140B2 (en) 2012-05-30 2014-03-25 Covidien Lp Surgical clamping device with ratcheting grip lock
CN109071634A (zh) 2016-04-26 2018-12-21 R.P.谢勒技术有限责任公司 抗体偶联物及其制备和使用方法

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JPS5630925A (en) * 1979-08-21 1981-03-28 Sumitomo Chem Co Ltd Purification of human growth hormone
DK309184D0 (da) * 1984-06-25 1984-06-25 Nordisk Insulinlab Fremgangsmaade til isolering af insulin eller insulinlignende materiale fra en fermenteringsvaeske
IT1223577B (it) * 1987-12-22 1990-09-19 Eniricerche Spa Procedimento migliorato per la preparazione dell'ormone della crescita umano naturale in forma pura
DD285113A5 (de) * 1989-06-21 1990-12-05 Akad Wissenschaften Ddr Verfahren zur gewinnung von reinem menschlichen wachstumshormon (hgh)
IL118201A (en) * 1995-05-11 2004-12-15 Roche Diagnostics Gmbh Preparation comprising a protein with human erythropoietin activity which is free of serum and non-recombinant mammalian protein and process for the preparation thereof
US6451347B1 (en) * 1999-03-01 2002-09-17 Alkermes Controlled Therapeutics, Inc. Method for purifying human growth hormone

Also Published As

Publication number Publication date
EA200602097A1 (ru) 2007-04-27
DE602004013847D1 (de) 2008-06-26
EP1809664A1 (en) 2007-07-25
CN101061137A (zh) 2007-10-24
CA2565209A1 (en) 2006-05-18
EP1809664B1 (en) 2008-05-14
US20080167452A1 (en) 2008-07-10
ATE395360T1 (de) 2008-05-15
WO2006051554A1 (en) 2006-05-18
AU2004324756A1 (en) 2006-05-18

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Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 4A,5A,6A E 7A ANUIDADE(S)

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2129 DE 25/10/2011.