BRPI0509168A - xilanases modificadas que exibem expressão melhorada - Google Patents

xilanases modificadas que exibem expressão melhorada

Info

Publication number
BRPI0509168A
BRPI0509168A BRPI0509168-3A BRPI0509168A BRPI0509168A BR PI0509168 A BRPI0509168 A BR PI0509168A BR PI0509168 A BRPI0509168 A BR PI0509168A BR PI0509168 A BRPI0509168 A BR PI0509168A
Authority
BR
Brazil
Prior art keywords
xylanase
modified
amino acid
expression
enhanced expression
Prior art date
Application number
BRPI0509168-3A
Other languages
English (en)
Inventor
Theresa White
Genevieve R Giroux
Katie E A Wallace
Original Assignee
Iogen Bio Products Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Iogen Bio Products Corp filed Critical Iogen Bio Products Corp
Publication of BRPI0509168A publication Critical patent/BRPI0509168A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2477—Hemicellulases not provided in a preceding group
    • C12N9/248—Xylanases
    • A—HUMAN NECESSITIES
    • A21—BAKING; EDIBLE DOUGHS
    • A21D—TREATMENT OF FLOUR OR DOUGH FOR BAKING, e.g. BY ADDITION OF MATERIALS; BAKING; BAKERY PRODUCTS
    • A21D8/00—Methods for preparing or baking dough
    • A21D8/02—Methods for preparing dough; Treating dough prior to baking
    • A21D8/04—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes
    • A21D8/042—Methods for preparing dough; Treating dough prior to baking treating dough with microorganisms or enzymes with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K10/00—Animal feeding-stuffs
    • A23K10/10—Animal feeding-stuffs obtained by microbiological or biochemical processes
    • A23K10/14—Pretreatment of feeding-stuffs with enzymes
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/30—Feeding-stuffs specially adapted for particular animals for swines
    • A—HUMAN NECESSITIES
    • A23—FOODS OR FOODSTUFFS; TREATMENT THEREOF, NOT COVERED BY OTHER CLASSES
    • A23K—FODDER
    • A23K50/00—Feeding-stuffs specially adapted for particular animals
    • A23K50/70—Feeding-stuffs specially adapted for particular animals for birds
    • A23K50/75—Feeding-stuffs specially adapted for particular animals for birds for poultry
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Polymers & Plastics (AREA)
  • Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Food Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Birds (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Animal Husbandry (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Physiology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Paper (AREA)

Abstract

XILANASES MODIFICADAS QUE EXIBEM EXPRESSãO MELHORADA. Uma enzima xilanase da Família 11 modificada compreendendo uma seqüência que introduz um sítio de glicosilação de consenso funcional é fornecida. Os exemplos não limitantes de sítios de glicosilação introduzidos incluem a mutação do aminoácido nas posições 34, 131, 180, 182, ou uma combinação destas, para uma asparagina. A posição de aminoácido indicada na xilanase da Família 11 é determinada a partir do alinhamento de seqüência da xilanase de interesse com aquele de uma seqüência de aminoá-cido da xilanase II de Trichoderma reesei.O sítio de glicosilação de consenso introduzido facilita a eficiência de expressão aumentada da xilanase modificada quando comparada á eficiência de expressão de uma xilanase correspondente a partir da qual a xilanase modificada foi derivada, usando cepas hospedeiras e condições de crescimento similares.
BRPI0509168-3A 2004-03-25 2005-03-24 xilanases modificadas que exibem expressão melhorada BRPI0509168A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55606104P 2004-03-25 2004-03-25
PCT/CA2005/000448 WO2005093072A1 (en) 2004-03-25 2005-03-24 Modified xylanases exhibiting improved expression

Publications (1)

Publication Number Publication Date
BRPI0509168A true BRPI0509168A (pt) 2007-09-11

Family

ID=35056207

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0509168-3A BRPI0509168A (pt) 2004-03-25 2005-03-24 xilanases modificadas que exibem expressão melhorada

Country Status (11)

Country Link
US (2) US7456005B2 (pt)
EP (1) EP1727903B1 (pt)
CN (1) CN101023173B (pt)
AR (1) AR048445A1 (pt)
AT (1) ATE527362T1 (pt)
BR (1) BRPI0509168A (pt)
CA (1) CA2560590C (pt)
ES (1) ES2377408T3 (pt)
RU (1) RU2394909C2 (pt)
WO (1) WO2005093072A1 (pt)
ZA (1) ZA200608088B (pt)

Families Citing this family (18)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2003208216A1 (en) * 2002-03-06 2003-09-16 Iogen Bio-Products Corporation Xylanase treatment of chemical pulp
WO2007115391A1 (en) 2006-04-12 2007-10-18 National Research Council Of Cananda Modification of xylanases to increase thermophilicity, thermostability and alkalophilicity
RU2464313C2 (ru) * 2006-04-12 2012-10-20 Нэйшенл Рисерч Каунсил Оф Кэнэда Модифицированная ксиланаза
CA2693084A1 (en) * 2007-06-08 2008-12-18 Benjamin S. Bower Heterologous and homologous cellulase expression system
DK2382310T3 (en) 2008-12-23 2016-12-12 Dupont Nutrition Biosci Aps POLYPEPTIDES HAVING xylanase activity
WO2010072226A1 (en) 2008-12-23 2010-07-01 Danisco A/S Polypeptides with xylanase activity
US9279112B2 (en) 2009-02-27 2016-03-08 Iogen Energy Corporation Cellulase enzymes having a modified linker and reduced lignin binding
WO2011097713A1 (en) 2010-02-11 2011-08-18 Iogen Energy Corporation Carbohydrate binding modules with reduced binding to lignin
US9034627B2 (en) 2010-03-01 2015-05-19 Toray Industries, Inc. Method for producing glucosidase, enzyme composition, and method for hydrolyzing biomass
JP5881234B2 (ja) 2010-07-14 2016-03-09 東レ株式会社 変異型βグルコシダーゼ、バイオマス分解用酵素組成物および糖液の製造方法
WO2015183710A1 (en) * 2014-05-30 2015-12-03 Novozymes A/S Variants of gh family 11 xylanase and polynucleotides encoding same
WO2016109422A1 (en) 2014-12-29 2016-07-07 Intercontinental Great Brands Llc Enzymatic bran and germ flavor and texture improvement
DK3481948T5 (da) * 2016-07-08 2024-09-02 Novozymes As Xylanasevarianter og polynukleotider, der koder for dem
CN107354165B (zh) * 2017-06-09 2019-02-26 北京工商大学 一种高产特异性产物的木聚糖酶改良基因及其工程菌制备低聚木糖的应用
EP3502242A1 (en) * 2017-12-20 2019-06-26 Technische Universität München New xylanase with improved thermostability and increased enzyme activity on arabinoxylan
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
CN115960869B (zh) * 2022-12-12 2024-01-30 华东理工大学 一种木聚糖酶及其分泌表达菌株的构建和应用
CN119020324A (zh) * 2024-08-14 2024-11-26 广西大学 突变木聚糖酶q156a-d201i及其应用

Family Cites Families (23)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5405769A (en) * 1993-04-08 1995-04-11 National Research Council Of Canada Construction of thermostable mutants of a low molecular mass xylanase
BE1008751A3 (fr) * 1994-07-26 1996-07-02 Solvay Xylanase, microorganismes la produisant, molecules d'adn, procedes de preparation de cette xylanase et utilisations de celle-ci.
US6300114B1 (en) * 1994-07-29 2001-10-09 Rohm Enzyme Finland Oy Sequences of xylanase and xylanase expression vectors
US6635464B1 (en) * 1995-12-18 2003-10-21 Rohm Enzyme Finland Oy Xylanases, genes encoding them, and uses thereof
RU2117703C1 (ru) * 1996-04-17 1998-08-20 Акционерное общество "Биотехнология" Мультиэнзимная композиция для животноводства
US5759840A (en) * 1996-09-09 1998-06-02 National Research Council Of Canada Modification of xylanase to improve thermophilicity, alkalophilicity and thermostability
US6015703A (en) * 1998-03-10 2000-01-18 Iogen Corporation Genetic constructs and genetically modified microbes for enhanced production of beta-glucosidase
EP0976838A1 (en) * 1998-05-06 2000-02-02 Rhone-Poulenc Nutrition Animale Enzymes mixture
DK1131447T4 (da) 1998-11-16 2011-12-19 Ca Nat Research Council Termostabile xylanaser
CN1266903A (zh) * 1999-03-15 2000-09-20 财团法人生物技术开发中心 重组木聚糖酶、其制备方法及应用
WO2001092487A2 (en) 2000-05-31 2001-12-06 National Research Council Of Canada Modified xylanases exhibiting increased thermophilicity and alkalophilicity
RU2201689C2 (ru) * 2000-06-27 2003-04-10 Кручина-Богданов Игорь Вадимович Способ производства кормов
AU2001267337A1 (en) * 2000-06-30 2002-01-14 Maxygen Aps Peptide extended glycosylated polypeptides
RU2291901C2 (ru) * 2000-09-21 2007-01-20 Басф Акциенгезелльшафт Полипептид и композиция, обладающие активностью ксиланазы, применение полипептида, полинуклеотид, кодирующий полипептид, вектор экспрессии, содержащий полинуклеотид, способ получения полинуклеотида, способ обработки растительного или ксилансодержащего материала
AU2002213838A1 (en) 2000-11-06 2002-05-15 Chr. Hansen A/S Method of producing non-bovine chymosin and use thereof
RU2201690C2 (ru) * 2001-03-13 2003-04-10 Всероссийский научно-исследовательский институт мясного скотоводства Способ повышения продуктивности кур-несушек
AU2002312627A1 (en) 2001-07-05 2003-01-21 The Regents Of The University Of California Nucleic acids encoding glucosinolate biosynthesis enzymes and methods of their use
US7510860B1 (en) 2001-11-21 2009-03-31 National Research Council Of Canada Xylanases with enhanced thermophilicity and alkalophilicity
RU2211242C2 (ru) * 2001-12-21 2003-08-27 Гусева Галина Валерьевна Способ производства пива (варианты)
DK1516053T3 (da) * 2002-06-14 2013-03-25 Verenium Corp Xylanaser, nucleinsyrer, som koder herfor, og fremgangsmåder til at fremstille og benytte disse
ES2707384T3 (es) * 2002-06-17 2019-04-03 Chr Hansen As Método mejorado de producción de una proteasa aspártica en un organismo huésped recombinante
CN1216998C (zh) * 2003-05-21 2005-08-31 江南大学 大幅度提高海栖热袍菌木聚糖酶b基因表达水平的技术方案
WO2006083240A2 (en) * 2003-12-19 2006-08-10 Syngenta Participations Ag Microbially expressed xylanases and their use as feed additives and other uses

Also Published As

Publication number Publication date
US7456005B2 (en) 2008-11-25
ZA200608088B (en) 2008-05-28
ES2377408T3 (es) 2012-03-27
EP1727903B1 (en) 2011-10-05
CA2560590A1 (en) 2005-10-06
US20050214410A1 (en) 2005-09-29
EP1727903A4 (en) 2008-04-23
AR048445A1 (es) 2006-04-26
RU2394909C2 (ru) 2010-07-20
CN101023173A (zh) 2007-08-22
EP1727903A1 (en) 2006-12-06
CN101023173B (zh) 2012-08-08
US20090111155A1 (en) 2009-04-30
CA2560590C (en) 2015-06-16
WO2005093072A1 (en) 2005-10-06
US7691609B2 (en) 2010-04-06
ATE527362T1 (de) 2011-10-15
RU2006137673A (ru) 2008-04-27

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE A 12A ANUIDADE.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: PROCEDIMENTO AUTOMATICO DE RECLASSIFICACAO. AS CLASSIFICACOES IPC ANTERIORES ERAM: C12N 15/56; C12N 9/24; C12N 1/15; A23K 1/165; A23L 1/03; D21H 17/22.

Ipc: C12N 9/24 (2006.01), A21D 8/04 (2006.01), A23K 10/

Ipc: C12N 9/24 (2006.01), A21D 8/04 (2006.01), A23K 10/