BRPI0509212A - proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma - Google Patents

proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma

Info

Publication number
BRPI0509212A
BRPI0509212A BRPI0509212-4A BRPI0509212A BRPI0509212A BR PI0509212 A BRPI0509212 A BR PI0509212A BR PI0509212 A BRPI0509212 A BR PI0509212A BR PI0509212 A BRPI0509212 A BR PI0509212A
Authority
BR
Brazil
Prior art keywords
cellulase fusion
cellulase
fusion protein
heterologous
same
Prior art date
Application number
BRPI0509212-4A
Other languages
English (en)
Inventor
Benjamin S Bower
Edmund A Larenas
Colin Mitchinson
Original Assignee
Genencor Int
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Genencor Int filed Critical Genencor Int
Publication of BRPI0509212A publication Critical patent/BRPI0509212A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091—Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/02—Fusion polypeptide containing a localisation/targetting motif containing a signal sequence

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biomedical Technology (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PROTEìNA DE FUSãO DE CELULASE E CONSTRUTO DE FUSãO DE CELULASE HETERóLOGO CODIFICANDO A MESMA. A presente invenção refere-se a um construto de fusão de celulase heterólogo que codifica uma proteina de fusão tendo atividade celulolítica compreendendo um primeiro domínio catalítico derivado de uma exocelobiodrolase catalítica e um segundo domínio catalítico derivado de uma inzima celulase. A invenção refere-se também a vetores e células hospedeiras fúngicas compreendendo o construto de fusão de celulase heterólogo bem como métodos para produção da dita proteína de fusão de celulase e composições de celulase enzimáticas.
BRPI0509212-4A 2004-03-25 2005-03-25 proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma BRPI0509212A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US55671104P 2004-03-25 2004-03-25
PCT/US2005/010242 WO2005093050A2 (en) 2004-03-25 2005-03-25 Cellulase fusion protein and heterologous cellulase fusion construct encoding the same

Publications (1)

Publication Number Publication Date
BRPI0509212A true BRPI0509212A (pt) 2007-08-28

Family

ID=35033698

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0509212-4A BRPI0509212A (pt) 2004-03-25 2005-03-25 proteìna de fusão de celulase e construto de fusão de celulase heterólogo codificando a mesma

Country Status (6)

Country Link
EP (2) EP2354222A1 (pt)
JP (1) JP5114194B2 (pt)
CN (1) CN1930294A (pt)
BR (1) BRPI0509212A (pt)
CA (1) CA2560588A1 (pt)
WO (1) WO2005093050A2 (pt)

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