BRPI0510611A - sistema de perfusão rápida e técnica de sujeição de placa utilizando um sistema de cámara de interface tendo exigências de baixo volume e elevado rendimento - Google Patents

sistema de perfusão rápida e técnica de sujeição de placa utilizando um sistema de cámara de interface tendo exigências de baixo volume e elevado rendimento

Info

Publication number
BRPI0510611A
BRPI0510611A BRPI0510611-7A BRPI0510611A BRPI0510611A BR PI0510611 A BRPI0510611 A BR PI0510611A BR PI0510611 A BRPI0510611 A BR PI0510611A BR PI0510611 A BRPI0510611 A BR PI0510611A
Authority
BR
Brazil
Prior art keywords
high throughput
low volume
interface chamber
plate clamping
throughput requirements
Prior art date
Application number
BRPI0510611-7A
Other languages
English (en)
Inventor
Dmytro Vasylyovich Vasylyev
Mark Robert Bowlby
Original Assignee
Wyeth Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Wyeth Corp filed Critical Wyeth Corp
Publication of BRPI0510611A publication Critical patent/BRPI0510611A/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/48707—Physical analysis of biological material of liquid biological material by electrical means
    • G01N33/48728—Investigating individual cells, e.g. by patch clamp, voltage clamp

Landscapes

  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Food Science & Technology (AREA)
  • Biochemistry (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • Biophysics (AREA)
  • Medicinal Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

SISTEMA DE PERFUSãO RáPIDA E TéCNICA DE SUJEIçãO DE PLACA UTILIZANDO UM SISTEMA DE CáMARA DE INTERFACE TENDO EXIGêNCIAS DE BAIXO VOLUME E ELEVADO RENDIMENTO. A presente invenção refere-se a um sistema para realizar perfusão rápida para as técnicas de sujeição de placa útil no estudo do efeito de compostos sobre canais de transferência de íon em tecido biológico. A invenção inclui adicionalmente conjuntos de câmara de microperfusão capazes de utilizar pequenas quantidades de material a ser testado e pequenas quantidades de veículo líquido, desse modo permitindo que múltiplos testes sejam concluídos em um curto período de tempo. A invenção refere-se mais amplamente a uma montagem de aplicação e manipulação de fármaco de eletrofisiologia para examinar produtos químicos como fármacos enquanto fornece rendimento elevado e baixos volumes de soluções e amostras.
BRPI0510611-7A 2004-05-03 2005-05-02 sistema de perfusão rápida e técnica de sujeição de placa utilizando um sistema de cámara de interface tendo exigências de baixo volume e elevado rendimento BRPI0510611A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/836,597 US20050241940A1 (en) 2004-05-03 2004-05-03 Fast perfusion system and patch clamp technique utilizing an interface chamber system having high throughput and low volume requirements
PCT/US2005/015064 WO2005108971A2 (en) 2004-05-03 2005-05-02 Fast perfusion system and patch clamp technique utilizing an interface chamber system having high throughput and low volume requirements

Publications (1)

Publication Number Publication Date
BRPI0510611A true BRPI0510611A (pt) 2007-10-30

Family

ID=35185963

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0510611-7A BRPI0510611A (pt) 2004-05-03 2005-05-02 sistema de perfusão rápida e técnica de sujeição de placa utilizando um sistema de cámara de interface tendo exigências de baixo volume e elevado rendimento

Country Status (9)

Country Link
US (1) US20050241940A1 (pt)
EP (1) EP1743164A2 (pt)
JP (1) JP2007535931A (pt)
CN (1) CN101076600A (pt)
AU (1) AU2005241475A1 (pt)
BR (1) BRPI0510611A (pt)
CA (1) CA2564511A1 (pt)
MX (1) MXPA06012574A (pt)
WO (1) WO2005108971A2 (pt)

Families Citing this family (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE102006052231A1 (de) * 2006-11-06 2008-05-08 Universität Wien Vorrichtungen und Verfahren für elektrophysiologische Zelluntersuchungen
EP2681304B1 (en) * 2011-03-03 2020-05-27 The Regents of The University of California Method of patterning cells on a substrate
CN102636550A (zh) * 2012-04-18 2012-08-15 南京师范大学 辣根细胞内外钙离子交换动态检测方法
CN102636551A (zh) * 2012-04-18 2012-08-15 南京师范大学 Hek 293细胞和红细胞内外钾离子交换动态检测方法
EP2708889A1 (en) * 2012-09-12 2014-03-19 Universität Leipzig Method and device for obtaining a tissue sample and for characterizing the tissue sample by determining at least one electrical property
CN113334266B (zh) * 2021-04-07 2023-03-28 中国科学院西北生态环境资源研究院 三轴压力室液压油中试后圆柱样取出夹具及使用方法
CN113406316B (zh) * 2021-06-17 2023-08-08 重庆医科大学附属儿童医院 一种电生理膜片钳灌流装置

Family Cites Families (6)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK0788600T3 (da) * 1994-10-28 2002-08-05 Sophion Bioscience As Patch-clamp-apparat og -teknik med høj gennemgang og lave fluidvolumenkrav
WO1997017426A1 (en) * 1995-11-08 1997-05-15 Trustees Of Boston University Cellular physiology workstations for automated data acquisition and perfusion control
NZ337814A (en) * 1997-05-01 2001-04-27 Neurosearch As Patch clamp apparatus with means for automatically connecting electrode to a cell
US20030139336A1 (en) * 2000-03-21 2003-07-24 Norwood James Henry Interface patch clamping
MXPA03000160A (es) * 2000-07-07 2003-09-22 Bristol Myers Squibb Co Configuracion de electrofisiologia adecuada para separacion por exclusion de alta produccion de compuestos para el descubrimiento de farmacos.
GB0128161D0 (en) * 2001-11-23 2002-01-16 Merck Sharp & Dohme Receptor protein

Also Published As

Publication number Publication date
US20050241940A1 (en) 2005-11-03
EP1743164A2 (en) 2007-01-17
JP2007535931A (ja) 2007-12-13
CA2564511A1 (en) 2005-11-17
MXPA06012574A (es) 2006-12-15
WO2005108971A3 (en) 2007-07-12
AU2005241475A1 (en) 2005-11-17
WO2005108971A2 (en) 2005-11-17
CN101076600A (zh) 2007-11-21

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Legal Events

Date Code Title Description
B08F Application fees: application dismissed [chapter 8.6 patent gazette]

Free format text: REFERENTE A 5A E 6A ANUIDADE(S).

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE A 8.6 NA RPI 2099 DE 29/03/2011.