BRPI0513773A - células tronco obtidas da polpa decìdua ou de dentes permanentes e dos germes dentais, capazes de produzir tecido ósseo humano - Google Patents

células tronco obtidas da polpa decìdua ou de dentes permanentes e dos germes dentais, capazes de produzir tecido ósseo humano

Info

Publication number
BRPI0513773A
BRPI0513773A BRPI0513773-0A BRPI0513773A BRPI0513773A BR PI0513773 A BRPI0513773 A BR PI0513773A BR PI0513773 A BRPI0513773 A BR PI0513773A BR PI0513773 A BRPI0513773 A BR PI0513773A
Authority
BR
Brazil
Prior art keywords
bone tissue
stem cells
osteoblasts
vitro
isolation
Prior art date
Application number
BRPI0513773-0A
Other languages
English (en)
Inventor
Franceso Carinci
Riccardo D Aquino
Alfredo De Rosa
Antonio Graziano
Gregorio Laino
Gianpaolo Papaccio
Original Assignee
Franceso Carinci
Riccardo D Aquino
Alfredo De Rosa
Antonio Graziano
Gregorio Laino
Gianpaolo Papaccio
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Franceso Carinci, Riccardo D Aquino, Alfredo De Rosa, Antonio Graziano, Gregorio Laino, Gianpaolo Papaccio filed Critical Franceso Carinci
Publication of BRPI0513773A publication Critical patent/BRPI0513773A/pt
Publication of BRPI0513773A8 publication Critical patent/BRPI0513773A8/pt

Links

Classifications

    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3804—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by specific cells or progenitors thereof, e.g. fibroblasts, connective tissue cells, kidney cells
    • A61L27/3821—Bone-forming cells, e.g. osteoblasts, osteocytes, osteoprogenitor cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61L—METHODS OR APPARATUS FOR STERILISING MATERIALS OR OBJECTS IN GENERAL; DISINFECTION, STERILISATION OR DEODORISATION OF AIR; CHEMICAL ASPECTS OF BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES; MATERIALS FOR BANDAGES, DRESSINGS, ABSORBENT PADS OR SURGICAL ARTICLES
    • A61L27/00—Materials for grafts or prostheses or for coating grafts or prostheses
    • A61L27/36—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix
    • A61L27/38—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells
    • A61L27/3839—Materials for grafts or prostheses or for coating grafts or prostheses containing ingredients of undetermined constitution or reaction products thereof, e.g. transplant tissue, natural bone, extracellular matrix containing added animal cells characterised by the site of application in the body
    • A61L27/3843—Connective tissue
    • A61L27/3847—Bones
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61P—SPECIFIC THERAPEUTIC ACTIVITY OF CHEMICAL COMPOUNDS OR MEDICINAL PREPARATIONS
    • A61P19/00—Drugs for skeletal disorders
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/06—Animal cells or tissues; Human cells or tissues
    • C12N5/0602—Vertebrate cells
    • C12N5/0652—Cells of skeletal and connective tissues; Mesenchyme
    • C12N5/0662—Stem cells
    • C12N5/0664—Dental pulp stem cells, Dental follicle stem cells
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K35/00—Medicinal preparations containing materials or reaction products thereof with undetermined constitution
    • A61K35/12—Materials from mammals; Compositions comprising non-specified tissues or cells; Compositions comprising non-embryonic stem cells; Genetically modified cells
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2509/00—Methods for the dissociation of cells, e.g. specific use of enzymes

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Chemical & Material Sciences (AREA)
  • Cell Biology (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Medicinal Chemistry (AREA)
  • Veterinary Medicine (AREA)
  • Public Health (AREA)
  • Animal Behavior & Ethology (AREA)
  • Organic Chemistry (AREA)
  • Botany (AREA)
  • Epidemiology (AREA)
  • Transplantation (AREA)
  • Orthopedic Medicine & Surgery (AREA)
  • Oral & Maxillofacial Surgery (AREA)
  • Dermatology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Developmental Biology & Embryology (AREA)
  • Biotechnology (AREA)
  • Wood Science & Technology (AREA)
  • Genetics & Genomics (AREA)
  • Urology & Nephrology (AREA)
  • Vascular Medicine (AREA)
  • Rheumatology (AREA)
  • Microbiology (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Nuclear Medicine, Radiotherapy & Molecular Imaging (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physical Education & Sports Medicine (AREA)
  • Materials For Medical Uses (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Coloring Foods And Improving Nutritive Qualities (AREA)

Abstract

CéLULAS TRONCO OBTIDAS DA POLPA DECìDUA OU DE DENTES PERMANENTES E DOS GERMES DENTAIS, CAPAZES DE PRODUZIR TECIDO óSSEO HUMANO. A presente invenção refere-se a um método que prevê o isolamento de uma nova subpopulação de células tronco derivadas da polpa dental, cuja diferenciação em osteoblastos leva à subseqüente produção e emprego de um tecido ósseo, denominado LAB (osso autólogo vivo, em inglês, Living Autologous Bone). Especificamente, a invenção descreve: 1) o isolamento de células tronco a partir da polpa de dentes decíduos e permanentes e de germes dentais, obtidas de pacientes humanos; 2) o crescimento destas células in vitro, sob condições específicas que permitem o isolamento de uma subpopulação celular, a qual, depois de diferenciação em osteoblastos, é capaz de produzir uma matriz extracelular in vitro, idêntica à detectável em tecido ósseo; 3) a aplicação desta população celular selecionada e diferenciada de modo a produzir tecido ósseo autólogo in vitro, contendo osteoblastos vitais; 4) a preservação do osso autólogo vivo sob condições as quais garantem a vitalidade celular; 5) a aplicação do osso autólogo vivo em pacientes doadores para reconstruir tecido ósseo, conforme requerido na prática diária na odontologia, na cirurgia maxilo-facial e na ortopedia.
BRPI0513773A 2004-07-28 2005-07-27 células tronco obtidas da polpa decídua ou de dentes permanentes e dos germes dentais, capazes de produzir tecido ósseo humano BRPI0513773A8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
IT000043A ITNA20040043A1 (it) 2004-07-28 2004-07-28 Tecnica di ingegneria tissutale ottenibile mediante isolamento di una nuova sottopopolazione di cellule staminali mbp-shed ed mbp-dpsc, isolate da polpa di denti decidui e permanenti in grado di produrre in vitro tessuto osseo umano.
PCT/EP2005/008136 WO2006010600A2 (en) 2004-07-28 2005-07-27 Stem cells obtained from pulp of deciduous or permanent teeth and of dental germ, able to produce human bone tissue

Publications (2)

Publication Number Publication Date
BRPI0513773A true BRPI0513773A (pt) 2008-05-13
BRPI0513773A8 BRPI0513773A8 (pt) 2018-05-08

Family

ID=35786558

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0513773A BRPI0513773A8 (pt) 2004-07-28 2005-07-27 células tronco obtidas da polpa decídua ou de dentes permanentes e dos germes dentais, capazes de produzir tecido ósseo humano

Country Status (14)

Country Link
US (1) US7955850B2 (pt)
EP (1) EP1773987B1 (pt)
JP (1) JP5179178B2 (pt)
AT (1) ATE503006T1 (pt)
BR (1) BRPI0513773A8 (pt)
CY (1) CY1111727T1 (pt)
DE (1) DE602005027076D1 (pt)
DK (1) DK1773987T3 (pt)
ES (1) ES2361527T3 (pt)
IT (1) ITNA20040043A1 (pt)
PL (1) PL1773987T3 (pt)
PT (1) PT1773987E (pt)
SI (1) SI1773987T1 (pt)
WO (1) WO2006010600A2 (pt)

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014107784A1 (pt) * 2013-01-14 2014-07-17 Muniz Ferreira Jose Ricardo Processo de coleta para congelamento e armazenamento de células tronco a partir da polpa de dentes decíduos
WO2017079813A1 (pt) * 2015-11-10 2017-05-18 Muniz Ferreira José Ricardo Processo de tratamento de células tronco provenientes da polpa de dentes decíduos com posterior criopreservação e descongelamento
WO2018064732A1 (pt) * 2016-10-03 2018-04-12 SILY DE ASSIS BUMACHAR, Brunela Processo de criopreservação de células-tronco obtidas a partir da polpa de dentes decíduos

Families Citing this family (24)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DE602005012081D1 (de) * 2005-07-29 2009-02-12 Univ Bonn Verfahren zur Isolierung von Stammzellen aus ein kissenformiges Gewebe der Zähne
DE102007005946A1 (de) * 2007-02-01 2008-08-14 Stiftung Caesar Therapeutische Zusammensetzung und Verwendung einer zellfreien Substanz
US8574567B2 (en) 2007-05-03 2013-11-05 The Brigham And Women's Hospital, Inc. Multipotent stem cells and uses thereof
EP2607477B1 (en) 2007-05-03 2020-09-23 The Brigham and Women's Hospital, Inc. Multipotent stem cells and uses thereof
US20110236977A1 (en) * 2008-04-02 2011-09-29 The Trustees Of Columbia University In The City Of New York Dental stem cell differentiation
ITRM20080342A1 (it) 2008-06-26 2009-12-27 Univ Degli Studi Udine Cellule di polpa dentale midollo-simili, metodi per isolamento ed uso.
WO2010026656A1 (ja) * 2008-09-05 2010-03-11 株式会社乳歯幹細胞バンク 再生医療に活用する良好な乳歯幹細胞を採取し長期間安定して冷凍保存するための総合的管理システム
US20100172951A1 (en) * 2009-01-03 2010-07-08 Ray Wasielewski Enhanced Medical Implant
US8470308B2 (en) 2009-01-03 2013-06-25 Ray C. Wasielewski Enhanced medical implant comprising disrupted tooth pulp and tooth particles
US10328103B2 (en) 2009-01-03 2019-06-25 Ray C. Wasielewski Medical treatment composition comprising mammalian dental pulp stem cells
US11078248B2 (en) * 2010-03-19 2021-08-03 Lifenet Health BMP peptides and methods of use
JPWO2011118795A1 (ja) 2010-03-26 2013-07-04 国立大学法人名古屋大学 損傷部治療用組成物
US10022205B2 (en) 2012-02-01 2018-07-17 Organ Technologies, Inc. Dental implant and method for producing same
WO2013146992A1 (ja) 2012-03-29 2013-10-03 日本ケミカルリサーチ株式会社 歯髄由来の多能性幹細胞の製造方法
JP6985703B2 (ja) 2016-08-24 2021-12-22 国立大学法人東海国立大学機構 精子活性化剤及びその用途
IT201700052399A1 (it) * 2017-06-06 2018-12-06 Costantino Santacroce Nuova metodica di separazione e crioconservazione della polpa dentaria per ottenere cellule staminali mesenchimali multipotenti
CN107475189B (zh) * 2017-10-12 2020-12-22 北京唐颐惠康生物医学技术有限公司 一种牙髓干细胞采集套装
KR20210041578A (ko) 2018-07-31 2021-04-15 제이씨알 파마 가부시키가이샤 치수 유래 세포의 제조 방법
EP4098267A4 (en) 2020-01-30 2024-03-06 JCR Pharmaceuticals Co., Ltd. MEDICINAL COMPOSITION COMPRISING CELLS DERIVED FROM DENTAL PULP
AU2021267900A1 (en) 2020-05-08 2023-01-19 Gallant Pet Inc. Uterine-derived regenerative cell compositions and uses thereof
CN112239744B (zh) * 2020-10-20 2021-08-03 青岛市妇女儿童医院（青岛市妇幼保健院、青岛市残疾儿童医疗康复中心、青岛市新生儿疾病筛查中心） 一种能够促进牙髓干细胞成骨转化的干扰rna及其应用
WO2024135673A1 (ja) 2022-12-20 2024-06-27 国立大学法人 東京大学 ナトリウム－ヨウ素共輸送体を発現する歯髄由来幹細胞
WO2025129376A1 (zh) * 2023-12-18 2025-06-26 冠昊生物科技股份有限公司 一种增强牙髓间充质干细胞免疫调节能力的方法
CN118497117B (zh) 2024-07-17 2024-11-12 广州医科大学附属口腔医院（广州医科大学羊城医院） G007-lk在促进牙源性间充质干细胞成骨分化和骨组织再生中的应用

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US5306305A (en) * 1992-01-31 1994-04-26 Etex Corporation Methods of coating implants with bony structure
AU774581B2 (en) * 1998-08-31 2004-07-01 New York University Stem cells bearing an FGF receptor on the cell surface
CA2416801C (en) * 2000-07-21 2012-01-03 The Government Of The United States Of America, Represented By The Secretary, Department Of Health And Human Services Adult human dental pulp stem cells in vitro and in vivo
AU2003231015B2 (en) * 2003-04-19 2009-10-08 Government Of The United States Of America As Represented By The Secretary Of The Department Of Health And Human Services National Institutes Of Health Postnatal stem cells and uses thereof

Cited By (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
WO2014107784A1 (pt) * 2013-01-14 2014-07-17 Muniz Ferreira Jose Ricardo Processo de coleta para congelamento e armazenamento de células tronco a partir da polpa de dentes decíduos
WO2017079813A1 (pt) * 2015-11-10 2017-05-18 Muniz Ferreira José Ricardo Processo de tratamento de células tronco provenientes da polpa de dentes decíduos com posterior criopreservação e descongelamento
WO2018064732A1 (pt) * 2016-10-03 2018-04-12 SILY DE ASSIS BUMACHAR, Brunela Processo de criopreservação de células-tronco obtidas a partir da polpa de dentes decíduos

Also Published As

Publication number Publication date
EP1773987A2 (en) 2007-04-18
DK1773987T3 (da) 2011-06-06
WO2006010600A3 (en) 2006-06-22
BRPI0513773A8 (pt) 2018-05-08
ES2361527T3 (es) 2011-06-17
JP5179178B2 (ja) 2013-04-10
PT1773987E (pt) 2011-05-25
DE602005027076D1 (de) 2011-05-05
US20090035376A1 (en) 2009-02-05
ATE503006T1 (de) 2011-04-15
PL1773987T3 (pl) 2011-08-31
ITNA20040043A1 (it) 2004-10-28
SI1773987T1 (sl) 2011-07-29
US7955850B2 (en) 2011-06-07
CY1111727T1 (el) 2015-10-07
EP1773987B1 (en) 2011-03-23
WO2006010600A2 (en) 2006-02-02
JP2008507962A (ja) 2008-03-21

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06H Technical and formal requirements: requirement cancelled [chapter 6.8 patent gazette]

Free format text: REF. A RPI NO 2153 DE 10/04/2012. ANULACAO DA EXIGENCIA 6.6 POR TER SIDO INDEVIDA. REQUERENTE NAO APRESENTOU RESPOSTA A EXIGENCIA CONFORME DISPOE A RESOLUCAO 207 DE 24/04/2009, ENTRETANTO, A RESPOSTA CONSTA NO FORMULARIO DE DEPOSITO.

B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa) [chapter 7.7 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: A61L 27/38 (2006.01), C12N 5/0775 (2010.01), A61K

B09B Patent application refused [chapter 9.2 patent gazette]
B15K Others concerning applications: alteration of classification

Ipc: C12N 5/0775 (2010.01), C12N 5/077 (2010.01), A61K

B09B Patent application refused [chapter 9.2 patent gazette]