BRPI0513965A - plantas transgênicas com expressão de celulase para autohidrólise de componentes de celulose e método para produção de açúcar solúvel - Google Patents

plantas transgênicas com expressão de celulase para autohidrólise de componentes de celulose e método para produção de açúcar solúvel

Info

Publication number
BRPI0513965A
BRPI0513965A BRPI0513965-1A BRPI0513965A BRPI0513965A BR PI0513965 A BRPI0513965 A BR PI0513965A BR PI0513965 A BRPI0513965 A BR PI0513965A BR PI0513965 A BRPI0513965 A BR PI0513965A
Authority
BR
Brazil
Prior art keywords
self
cellulose
hydrolysis
transgenic
transgenic plants
Prior art date
Application number
BRPI0513965-1A
Other languages
English (en)
Inventor
Hyeun-Jong Bae
Inhwan Hwang
Ginette Turcotte
Serge Laberge
Original Assignee
Postech Foundation
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Postech Foundation filed Critical Postech Foundation
Publication of BRPI0513965A publication Critical patent/BRPI0513965A/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/113—Non-coding nucleic acids modulating the expression of genes, e.g. antisense oligonucleotides; Antisense DNA or RNA; Triplex- forming oligonucleotides; Catalytic nucleic acids, e.g. ribozymes; Nucleic acids used in co-suppression or gene silencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00—Preparation of compounds containing saccharide radicals
    • C12P19/14—Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C12N15/69—Increasing the copy number of the vector
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8243—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine
    • C12N15/8245—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits involving biosynthetic or metabolic pathways, i.e. metabolic engineering, e.g. nicotine, caffeine involving modified carbohydrate or sugar alcohol metabolism, e.g. starch biosynthesis
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase

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  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Nutrition Science (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Enzymes And Modification Thereof (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

PLANTAS TRANSGêNICAS COM EXPRESSãO DE CELULASE PARA AUTOHIDRóLISE DE COMPONENTES DE CELULOSE E MéTODO PARA PRODUçãO DE AçúCAR SOLúVEL. A presente invenção se relaciona com plantas transgênicas para produzir celulose por um mecanismo de autohidrólise e com um método para produção de açúcares solúveis que utiliza as mesmas, mais particularmente com plantas transgênicas transformadas com cDNA recombinante que codifica a celulose, o domínio de ligação à celulose e o peptídeo de direcionamento para o cloroplasto, e um método para produzir açúcares solúveis pelo uso das mesmas. As plantas transgênicas e um método de produção de açúcares solúveis que utiliza as mesmas seriam um sistema muito eficaz em termos de custo para a produção de açúcares solúveis em grande escala.
BRPI0513965-1A 2004-07-30 2005-07-30 plantas transgênicas com expressão de celulase para autohidrólise de componentes de celulose e método para produção de açúcar solúvel BRPI0513965A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020040060618A KR100769584B1 (ko) 2004-07-30 2004-07-30 셀룰로오스의 자가가수분해를 위한 셀룰라아제 발현형질전환 식물체 및 이를 이용한 수용성 당의 생산방법
PCT/KR2005/002494 WO2006011779A1 (en) 2004-07-30 2005-07-30 Transgenic plants expressing cellulase for autohydrolysis of cellulose components and method for production of soluble sugar

Publications (1)

Publication Number Publication Date
BRPI0513965A true BRPI0513965A (pt) 2008-05-20

Family

ID=35786477

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0513965-1A BRPI0513965A (pt) 2004-07-30 2005-07-30 plantas transgênicas com expressão de celulase para autohidrólise de componentes de celulose e método para produção de açúcar solúvel

Country Status (8)

Country Link
US (1) US7786345B2 (pt)
EP (1) EP1789550A4 (pt)
KR (1) KR100769584B1 (pt)
CN (1) CN101001952B (pt)
BR (1) BRPI0513965A (pt)
CA (1) CA2575717A1 (pt)
NZ (1) NZ553435A (pt)
WO (1) WO2006011779A1 (pt)

Families Citing this family (19)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US20090221039A1 (en) * 2006-04-06 2009-09-03 Institut Francias Du Petrole Fusion proteins between plant cell-wall degrading enzymes and a swollenin, and their uses
KR101137020B1 (ko) * 2006-12-15 2012-04-19 한국생명공학연구원 호열성 미생물 유래의 셀룰라아제 유전자
US20090205075A1 (en) * 2008-01-30 2009-08-13 Stacy Miles Use of plastid transit peptides derived from glaucocystophytes
KR101262300B1 (ko) 2009-10-06 2013-05-20 헬릭스 주식회사 형질전환 식물 유래의 고병원성 조류독감 바이러스 단백질 백신 및 그 제조방법
EP2585606A4 (en) 2010-06-26 2016-02-17 Virdia Ltd SUGAR MIXTURES, METHODS OF PRODUCTION AND USE THEREOF
IL206678A0 (en) 2010-06-28 2010-12-30 Hcl Cleantech Ltd A method for the production of fermentable sugars
IT1406669B1 (it) * 2010-12-03 2014-03-07 Longoni Produzione di cellulasi ricombinanti di chaetamium globosum in piante transplastomiche di tabacco
US9512495B2 (en) 2011-04-07 2016-12-06 Virdia, Inc. Lignocellulose conversion processes and products
GB2518547B (en) 2012-05-03 2015-10-07 Virdia Ltd Method for the preparation of high purity lignin
KR101678067B1 (ko) * 2013-02-15 2016-11-21 고려대학교 산학협력단 클로스트리디움 셀룰로보란스 유래 엔도글루카나아제를 포함하는 셀룰로좀 복합체 및 그의 용도
US10260081B2 (en) 2013-03-15 2019-04-16 Edeniq, Inc. Cellulosic enzyme recycling from separation of saccharified biomass
US9487770B2 (en) 2013-04-26 2016-11-08 Korea University Research And Business Foundation Recombinant cellulase and use thereof
CN112226466A (zh) 2015-01-07 2021-01-15 威尔迪亚公司 萃取和转化半纤维素糖的方法
BR112017025322A8 (pt) 2015-05-27 2022-08-23 Virdia Inc Processos integrados para recuperação de hidrolisato celulósico após hidrólise de polpa de celulose
WO2017195919A1 (ko) * 2016-05-12 2017-11-16 주식회사 바이오앱 식물 유래의 돼지 열병 백신용 조성물 및 이의 제조방법
KR101957561B1 (ko) * 2016-05-20 2019-03-12 포항공과대학교 산학협력단 RbcS 융합 단백질을 이용하여 식물로부터 목적 단백질을　고발현하는 방법 및 목적 단백질 발현 식물체를 이용한 의료용 단백질의 경구투여용 조성물의 제조 방법
WO2020101187A1 (ko) * 2018-11-15 2020-05-22 주식회사 바이오앱 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법
KR102288367B1 (ko) 2018-11-15 2021-08-11 주식회사 바이오앱 식물체에서 바이러스-유사 입자를 발현하는 재조합 벡터 및 이를 이용한 써코바이러스-유사 입자를 포함하는 백신 조성물의 제조방법
KR102232535B1 (ko) * 2018-11-16 2021-03-26 주식회사 포스코 재조합 ccmv 외피 단백질을 발현시키는 재조합 벡터 및 이를 이용하여 재조합 ccmv 바이러스유사입자를 획득하는 방법

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CA1340766C (en) * 1984-12-24 1999-09-28 Clive Waldron Selectable marker for development of vectors and transformation systems in plants
KR20000036077A (ko) * 1996-09-12 2000-06-26 한스 루돌프 하우스, 헨리테 브룬너, 베아트리체 귄터 셀룰로스 분해 효소를 발현하는 트랜스젠 식물
AU754791B2 (en) * 1998-09-04 2002-11-28 University Of Georgia Research Foundation, Inc., The Phenolic acid esterases, coding sequences and methods
AU5279100A (en) * 1999-05-19 2000-12-05 Midwest Research Institute E1 endoglucanase variants y245g, y82r and w42r
US6429359B1 (en) * 1999-09-02 2002-08-06 Arch Development Corporation Production of cellulase in plastids of transgenic plants
AU2002211798A1 (en) * 2000-10-20 2002-05-06 Michigan State University Transgenic plants containing ligninase and cellulase which degrade lignin and cellulose to fermentable sugars
WO2003049538A2 (en) * 2001-12-06 2003-06-19 Prodigene, Inc. Methods for the cost-effective saccharification of lignocellulosic biomass
US20040005674A1 (en) * 2002-04-30 2004-01-08 Athenix Corporation Methods for enzymatic hydrolysis of lignocellulose

Also Published As

Publication number Publication date
US7786345B2 (en) 2010-08-31
CN101001952A (zh) 2007-07-18
KR20060011675A (ko) 2006-02-03
CN101001952B (zh) 2011-06-29
KR100769584B1 (ko) 2007-10-23
US20070226840A1 (en) 2007-09-27
WO2006011779A1 (en) 2006-02-02
EP1789550A1 (en) 2007-05-30
CA2575717A1 (en) 2006-02-02
NZ553435A (en) 2009-12-24
EP1789550A4 (en) 2008-07-02

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Ipc: C12N 15/56 (2006.01), C12N 15/82 (2006.01), A01H 5

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

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