BRPI0514376A - promotores e terminadores de transcrição gênica isolados de fungos e métodos para a produção de proteìna e composto quìmico em um fungo - Google Patents

promotores e terminadores de transcrição gênica isolados de fungos e métodos para a produção de proteìna e composto quìmico em um fungo

Info

Publication number
BRPI0514376A
BRPI0514376A BRPI0514376-4A BRPI0514376A BRPI0514376A BR PI0514376 A BRPI0514376 A BR PI0514376A BR PI0514376 A BRPI0514376 A BR PI0514376A BR PI0514376 A BRPI0514376 A BR PI0514376A
Authority
BR
Brazil
Prior art keywords
fungus
gene transcription
protein
production
gene
Prior art date
Application number
BRPI0514376-4A
Other languages
English (en)
Inventor
Ziyu Dai
Linda L Lasure
Jon K Magnuson
Original Assignee
Battelle Memorial Institute
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Battelle Memorial Institute filed Critical Battelle Memorial Institute
Publication of BRPI0514376A publication Critical patent/BRPI0514376A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/38—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from Aspergillus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P17/00—Preparation of heterocyclic carbon compounds with only O, N, S, Se or Te as ring hetero atoms
    • C12P17/02—Oxygen as only ring hetero atoms
    • C12P17/06—Oxygen as only ring hetero atoms containing a six-membered hetero ring, e.g. fluorescein
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/005—Glycopeptides, glycoproteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P35/00—Preparation of compounds having a 5-thia-1-azabicyclo [4.2.0] octane ring system, e.g. cephalosporin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P37/00—Preparation of compounds having a 4-thia-1-azabicyclo [3.2.0] heptane ring system, e.g. penicillin
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/48—Tricarboxylic acids, e.g. citric acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/58—Aldonic, ketoaldonic or saccharic acids

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

PROMOTORES E TERMINADORES DA TRANSCRIçãO GêNICA ISOLADOS DE FUNGOS E MéTODOS PARA A PRODUçãO DE PROTEìNA E COMPOSTO QUìMICO EM UM FUNGO A presente invenção engloba elementos regulatórios de genes isolados e terminadores da transcrição de gene que são diferencialmente expressos em um fungo nativo exibindo uma primeira morfologia filamentosa em relação ao fungo nativo exibindo uma segunda morfologia. A invenção também engloba um método de utilização de um fungo para a produção de proteína ou composto químico. Um fungo transformado é produzido pela transformação de um fungo com uma molécula de polinucleotídeo recombinante. A molécula de polinucleotídeo recombinante contém uma seqüência de polinucleotídeo isolada operavelmente ligada a outra molécula compreendendo uma região codificante de um gene de interesse. O elemento regulatório do gene e o terminador da transcrição do gene podem temporariamente e espacialmente regular a expressão de genes em particular para a produção ótima dos compostos de interesse em um fungo transgênico.
BRPI0514376-4A 2004-08-17 2005-08-12 promotores e terminadores de transcrição gênica isolados de fungos e métodos para a produção de proteìna e composto quìmico em um fungo BRPI0514376A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US10/920,625 US7449569B2 (en) 2004-08-17 2004-08-17 Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
PCT/US2005/028651 WO2007032751A2 (en) 2004-08-17 2005-08-12 Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Publications (1)

Publication Number Publication Date
BRPI0514376A true BRPI0514376A (pt) 2008-06-10

Family

ID=35910074

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0514376-4A BRPI0514376A (pt) 2004-08-17 2005-08-12 promotores e terminadores de transcrição gênica isolados de fungos e métodos para a produção de proteìna e composto quìmico em um fungo

Country Status (10)

Country Link
US (2) US7449569B2 (pt)
EP (2) EP1799704B1 (pt)
JP (1) JP2008510495A (pt)
KR (1) KR20080028829A (pt)
AT (1) ATE482278T1 (pt)
BR (1) BRPI0514376A (pt)
CA (1) CA2573130A1 (pt)
DE (1) DE602005023756D1 (pt)
DK (1) DK1799704T3 (pt)
WO (1) WO2007032751A2 (pt)

Families Citing this family (4)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US7449569B2 (en) 2004-08-17 2008-11-11 Battelle Memorial Institute Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus
US8454906B2 (en) * 2007-07-24 2013-06-04 The Regents Of The University Of California Microfabricated droplet generator for single molecule/cell genetic analysis in engineered monodispersed emulsions
CN102517265B (zh) * 2011-12-30 2014-05-28 中国农业大学 一种酯酶及其制备方法和应用
CN105087521B (zh) * 2014-05-21 2018-02-09 东莞泛亚太生物科技有限公司 具提升酶产量及活性之甘露聚糖酶

Family Cites Families (3)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US442017A (en) * 1890-12-02 Ore distributer and sampler
US7439067B2 (en) * 2002-05-20 2008-10-21 Battelle Memorial Institute Isolated polynucleotides and methods of promoting a morphology in a fungus
US7449569B2 (en) 2004-08-17 2008-11-11 Battelle Memorial Institute Isolated fungal promoters and gene transcription terminators and methods of protein and chemical production in a fungus

Also Published As

Publication number Publication date
JP2008510495A (ja) 2008-04-10
US7449569B2 (en) 2008-11-11
DK1799704T3 (da) 2010-12-13
DE602005023756D1 (de) 2010-11-04
US8735562B2 (en) 2014-05-27
US20090068723A1 (en) 2009-03-12
EP2267134A1 (en) 2010-12-29
WO2007032751A3 (en) 2007-08-09
WO2007032751A2 (en) 2007-03-22
CA2573130A1 (en) 2006-02-17
EP1799704B1 (en) 2010-09-22
EP1799704A2 (en) 2007-06-27
US20060040342A1 (en) 2006-02-23
ATE482278T1 (de) 2010-10-15
KR20080028829A (ko) 2008-04-01

Similar Documents

Publication Publication Date Title
DK1831362T3 (da) Syreaktive svampeproteaser
AR127516A2 (es) VARIANTES DE Fc Y MÉTODOS PARA SU PRODUCCIÓN
WO2008074891A3 (en) Plants having enhanced yield-related traits and a method for method for making the same
DK1928901T3 (da) Polypeptider, der har beta-glucosidaseaktivitet og polynukleotider, der koder for samme
EP2298872A3 (en) Polypeptides having lipase activity and polynucleotides encoding same
WO2006042158A3 (en) Methods and compositions for improving recombinant protein production
DE602006019050D1 (de) Polypeptide mit cellobiohydrolaseaktivität und dafür kodierende polynukleotide
WO2005081714A3 (en) Use of interfering rna in the production of transgenic animals
WO2006066203A3 (en) Small interfering rna (sirna) molecules for modulating superoxide dismutase (sod)
EA201290930A1 (ru) Растения с улучшенными характеристиками урожайности и способ их получения
WO2006047469A8 (en) Polypeptides having lipase activity and polynucleotides encoding same
WO2005062918A3 (en) Methods of treating asthma
ATE515568T1 (de) Polypeptide mit endoglucanase-aktivtät und dafür codierende polynukleotide
BRPI0514376A (pt) promotores e terminadores de transcrição gênica isolados de fungos e métodos para a produção de proteìna e composto quìmico em um fungo
ATE419340T1 (de) Neue carbonylreduktase, deren gen sowie verfahren zur verwendung davon
WO2005110067A3 (en) Method and compositions for rna interference
ATE463572T1 (de) Rekombinanter faktor viii mit erhöhter spezifischer aktivität
WO2008017073A3 (en) Vector systems
WO2005040215A3 (en) Ovomucoid promoters and mehtods of use
DE602005024868D1 (de) Neuer l-lysin-induzierbarer promotor
DK1756273T3 (da) Signalpeptid til frembringelse af et polypeptid
ATE534743T1 (de) Promotoren mit veränderter transkriptionseffizienz aus der methylotrophen hefe hansenula polymorpha
DK2066790T3 (da) Galactosyltransferase
WO2021231549A3 (en) Engineered gene-editing proteins
ATE430191T1 (de) Überexprimierte und gereinigte aspergillus ficuum oxidase und nukleinsäuren die für sie kodieren

Legal Events

Date Code Title Description
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]

Free format text: REFERENTE AS 5A E 6A ANUIDADES.

B08K Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette]

Free format text: REFERENTE AO DESPACHO 8.6 PUBLICADO NA RPI 2160 DE 29/05/2012.