BRPI0515547A - incorporação in vivo de aminoácidos alquinil em proteìnas em eubacterias - Google Patents

incorporação in vivo de aminoácidos alquinil em proteìnas em eubacterias

Info

Publication number
BRPI0515547A
BRPI0515547A BRPI0515547-9A BRPI0515547A BRPI0515547A BR PI0515547 A BRPI0515547 A BR PI0515547A BR PI0515547 A BRPI0515547 A BR PI0515547A BR PI0515547 A BRPI0515547 A BR PI0515547A
Authority
BR
Brazil
Prior art keywords
amino acids
proteins
eubacteria
alkynyl amino
vivo incorporation
Prior art date
Application number
BRPI0515547-9A
Other languages
English (en)
Inventor
Alexander Deiters
Peter Schultz
Original Assignee
Scripps Research Inst
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Scripps Research Inst filed Critical Scripps Research Inst
Publication of BRPI0515547A publication Critical patent/BRPI0515547A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/195—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from bacteria
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K47/00—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient
    • A61K47/50—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates
    • A61K47/51—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent
    • A61K47/62—Medicinal preparations characterised by the non-active ingredients used, e.g. carriers or inert additives; Targeting or modifying agents chemically bound to the active ingredient the non-active ingredient being chemically bound to the active ingredient, e.g. polymer-drug conjugates the non-active ingredient being a modifying agent the modifying agent being a protein, peptide or polyamino acid
    • A61K47/64—Drug-peptide, drug-protein or drug-polyamino acid conjugates, i.e. the modifying agent being a peptide, protein or polyamino acid which is covalently bonded or complexed to a therapeutically active agent
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07H—SUGARS; DERIVATIVES THEREOF; NUCLEOSIDES; NUCLEOTIDES; NUCLEIC ACIDS
    • C07H21/00—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids
    • C07H21/04—Compounds containing two or more mononucleotide units having separate phosphate or polyphosphate groups linked by saccharide radicals of nucleoside groups, e.g. nucleic acids with deoxyribosyl as saccharide radical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/67—General methods for enhancing the expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/93—Ligases (6)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biomedical Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Biophysics (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Epidemiology (AREA)
  • Animal Behavior & Ethology (AREA)
  • Public Health (AREA)
  • Veterinary Medicine (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Peptides Or Proteins (AREA)
  • Enzymes And Modification Thereof (AREA)

Abstract

"INCORPORAçãO IN VIVO DE AMINOáCIDOS ALQUINIL EM PROTEìNAS EM EUBACTERIAS". A invenção se relaciona a pares ortogonais de tR-NAs e aminoacil-tRNA sintetases que podem incorporar aminoácidos alquinil como para-propargiloxifenilalanina em proteínas produzidas em um hospedeiro Eubactéria como E. coli. A invenção fornece novas sintetases ortogonais, métodos para a identificação e fabricação das novas sintetases, métodos para a produção de proteínas contendo aminoácidos alquinil e sistemas de tradução celular.
BRPI0515547-9A 2004-09-21 2005-09-20 incorporação in vivo de aminoácidos alquinil em proteìnas em eubacterias BRPI0515547A (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US61222004P 2004-09-21 2004-09-21
US63087604P 2004-11-24 2004-11-24
US63415104P 2004-12-07 2004-12-07
PCT/US2005/033784 WO2006034332A2 (en) 2004-09-21 2005-09-20 In vivo incorporation of alkynyl amino acids into proteins in eubacteria

Publications (1)

Publication Number Publication Date
BRPI0515547A true BRPI0515547A (pt) 2008-07-29

Family

ID=36090646

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0515547-9A BRPI0515547A (pt) 2004-09-21 2005-09-20 incorporação in vivo de aminoácidos alquinil em proteìnas em eubacterias

Country Status (13)

Country Link
US (2) US7824893B2 (pt)
EP (2) EP1794323B1 (pt)
JP (2) JP2008513039A (pt)
CN (1) CN101151366B (pt)
AU (1) AU2005286722B2 (pt)
BR (1) BRPI0515547A (pt)
CA (1) CA2580840A1 (pt)
ES (2) ES2666693T3 (pt)
IL (3) IL182035A0 (pt)
MX (1) MX2007003142A (pt)
PL (1) PL1794323T3 (pt)
PT (1) PT1794323E (pt)
WO (1) WO2006034332A2 (pt)

Families Citing this family (41)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2322631T3 (en) * 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
CA2500653A1 (en) * 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
ES2301099T3 (es) * 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2006036779A2 (en) * 2004-09-22 2006-04-06 The Scripps Research Institute Site-specific labeling of proteins for nmr studies
WO2006110182A2 (en) 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids
US8367588B2 (en) * 2005-10-12 2013-02-05 The Scripps Research Institute Selective posttranslational modification of phage-displayed polypeptides
US7776535B2 (en) * 2006-02-06 2010-08-17 Franklin And Marshall College Site-specific incorporation of fluorinated amino acids into proteins
ES2432391T3 (es) * 2006-03-09 2013-12-03 The Scripps Research Institute Sistema para la expresión de componentes de traducción ortogonales en células huésped eubacterianas
BRPI0709603A2 (pt) 2006-03-16 2011-07-19 Scripps Research Inst expressão de proteìnas geneticamente programada contendo o aminoácido não natural fenilselenocisteìna
CA2652894A1 (en) * 2006-05-23 2007-12-06 The Scripps Research Institute Genetically encoded fluorescent coumarin amino acids
EP2052083B1 (en) 2006-09-21 2014-01-08 The Scripps Research Institute Genetically programmed expression of selectively sulfated proteins in eubacteria
EP2069481A4 (en) 2006-10-18 2010-01-06 Scripps Research Inst GENETIC ASSEMBLY OF UNNATURAL AMINO ACIDS IN PROTEINS IN MAMMALIAN CELLS
CA2703736A1 (en) 2007-10-25 2009-04-30 The Scripps Research Institute Genetic incorporation of 3-aminotyrosine into reductases
US20090148887A1 (en) * 2007-11-02 2009-06-11 The Scripps Research Institute Genetically encoded boronate amino acid
CA2704494A1 (en) * 2007-11-02 2009-05-14 The Scripps Research Institute Directed evolution using proteins comprising unnatural amino acids
EP2889370A1 (en) 2007-12-11 2015-07-01 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast Pichia pastoris
JP2011514322A (ja) * 2008-02-08 2011-05-06 ザ スクリプス リサーチ インスティチュート 遺伝的にコードされる非天然アミノ酸による免疫寛容解除
CA2975841C (en) 2008-04-30 2021-05-25 Siemens Medical Solutions Usa, Inc. Substrate based pet imaging agents
JP5959201B2 (ja) 2008-12-10 2016-08-02 ザ スクリプス リサーチ インスティテュート 化学的に反応性の非天然アミノ酸を使用するキャリア−ペプチド接合体の製造
US20090197339A1 (en) * 2008-12-10 2009-08-06 The Scripps Research Institute In vivo unnatural amino acid expression in the methylotrophic yeast pichia pastoris
GB2470770A (en) * 2009-06-04 2010-12-08 Medical Res Council Incorporation of unnatural amino acids having an orthogonal functional group into polypeptides using orthogonal tRNA/tRNA synthetase pairs
US8828395B2 (en) 2009-12-11 2014-09-09 Atyr Pharma, Inc. Antibodies that bind tyrosyl-tRNA synthetases
AU2011293294B2 (en) 2010-08-25 2016-03-24 Pangu Biopharma Limited Innovative discovery of therapeutic, diagnostic, and antibody compositions related to protein fragments of Tyrosyl-tRNA synthetases
US9714419B2 (en) 2011-08-09 2017-07-25 Atyr Pharma, Inc. PEGylated tyrosyl-tRNA synthetase polypeptides
CA2873793C (en) 2012-05-18 2022-02-22 Medical Research Council Methods of incorporating an amino acid comprising a bcn group into a polypeptide using an orthogonal codon encoding it and an orthorgonal pylrs synthase
CN103571804B (zh) * 2012-08-10 2015-08-12 中国科学院生物物理研究所 3-吡唑基酪氨酸翻译系统及其应用
JP2016507520A (ja) 2013-01-23 2016-03-10 ザ ボード オブ トラスティーズ オブ ザ レランド スタンフォード ジュニア ユニバーシティー 安定化されたｂ型肝炎コアポリペプチド
US9255255B2 (en) 2013-03-04 2016-02-09 The Board Of Trustees Of The Leland Stanford Junior University Synthesis of linear and branched polymers of polypeptides through direct conjugation
UY35368A (es) 2013-03-08 2014-10-31 Irm Llc Péptidos y composiciones para el tratamiento de daño articular
US10005838B2 (en) 2013-07-19 2018-06-26 The Regents Of The University Of California Milk fat globule epidermal growth factor 8 regulates fatty acid uptake
GB2528227A (en) 2014-03-14 2016-01-20 Medical Res Council Cyclopropene amino acids and methods
CN106084017B (zh) * 2016-06-13 2019-11-22 浙江大学 一种大肠杆菌水通道蛋白AQPZ中整合非天然氨基酸pPpa的方法
CN117860868A (zh) 2016-11-14 2024-04-12 诺华股份有限公司 用于治疗软骨损伤和关节炎的方法和组合物
EP3538546B1 (en) 2016-11-14 2025-01-08 Novartis AG Compositions, methods, and therapeutic uses related to fusogenic protein minion
GB201817444D0 (en) 2018-10-26 2018-12-12 Res & Innovation Uk Methods and compositions
JP7495420B2 (ja) * 2019-02-20 2024-06-04 ノヴォ ノルディスク アー／エス アミノアシル－ｔＲＮＡ合成酵素およびこの使用
KR102152939B1 (ko) * 2019-04-30 2020-09-07 한국화학연구원 공유결합성 트리아졸로 연결된 가시광선 흡수 광촉매, 이의 제조방법 및 이를 이용한 아자이드-알카인 고리화 첨가반응으로 트리아졸 유도체를 제조하는 방법
EP4196610A1 (en) * 2020-08-12 2023-06-21 Cambridge Enterprise Limited Method for targeted nucleic acid cleavage
CN114107394B (zh) * 2021-11-05 2024-01-30 中国科学院精密测量科学与技术创新研究院 一种慢病毒转移载体、表达PylRS及tRNACUA的细胞系及制备方法与应用
CN116376851A (zh) * 2022-08-25 2023-07-04 凯莱英医药集团（天津）股份有限公司 氨酰tRNA合酶突变体及其应用

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
DK2322631T3 (en) * 2001-04-19 2015-01-12 Scripps Research Inst Methods and compositions for the production of orthogonal tRNA-aminoacyl-tRNA syntetasepar
US7432092B2 (en) 2002-10-16 2008-10-07 The Scripps Research Institute Site specific incorporation of keto amino acids into proteins
CA2500653A1 (en) 2002-10-16 2004-04-29 The Scripps Research Institute Glycoprotein synthesis
EP1583816A4 (en) 2002-12-22 2007-06-13 Scripps Research Inst PROTEIN ARRAYS
KR101171397B1 (ko) 2003-04-17 2012-08-07 더 스크립스 리서치 인스티튜트 진핵 유전자 코드의 확장
CA2527877A1 (en) * 2003-06-18 2005-01-13 The Scripps Research Institute Unnatural reactive amino acid genetic code additions
EP1658366A4 (en) 2003-07-07 2006-08-09 Scripps Research Inst COMPOSITIONS OF ORTHOGONAL GLUTAMYL-TRNA AND AMINOACYL-TRNA-SYNTHETASEPAARES AND THEIR USES
WO2005007870A2 (en) 2003-07-07 2005-01-27 The Scripps Research Institute COMPOSITIONS OF ORTHOGONAL LEUCYL-tRNA AND AMINOACYL-tRNA SYNTHETASE PAIRS AND USES THEREOF
ES2301099T3 (es) 2003-07-07 2008-06-16 The Scripps Research Institute Composiciones de parejas ortogonales lisil-arnt y aminoacil-arnt sintetasa y usos de las mismas.
WO2006110182A2 (en) 2004-10-27 2006-10-19 The Scripps Research Institute Orthogonal translation components for the in vivo incorporation of unnatural amino acids

Also Published As

Publication number Publication date
IL218090A0 (en) 2012-03-29
WO2006034332A3 (en) 2007-12-06
CN101151366B (zh) 2012-12-19
PT1794323E (pt) 2014-10-30
EP1794323A4 (en) 2008-08-27
ES2666693T3 (es) 2018-05-07
WO2006034332A2 (en) 2006-03-30
JP2013009682A (ja) 2013-01-17
US20080220472A1 (en) 2008-09-11
PL1794323T3 (pl) 2015-02-27
US7718410B2 (en) 2010-05-18
AU2005286722B2 (en) 2010-12-02
IL218089A0 (en) 2012-03-29
CN101151366A (zh) 2008-03-26
AU2005286722A1 (en) 2006-03-30
ES2519444T3 (es) 2014-11-07
IL218089A (en) 2014-01-30
US7824893B2 (en) 2010-11-02
US20060073507A1 (en) 2006-04-06
IL218090A (en) 2014-02-27
EP1794323A2 (en) 2007-06-13
EP1794323B1 (en) 2014-08-06
JP2008513039A (ja) 2008-05-01
EP2770053B1 (en) 2018-02-21
IL182035A0 (en) 2007-07-24
JP5823941B2 (ja) 2015-11-25
MX2007003142A (es) 2007-07-04
EP2770053A1 (en) 2014-08-27
CA2580840A1 (en) 2006-03-30

Similar Documents

Publication Publication Date Title
BRPI0515547A (pt) incorporação in vivo de aminoácidos alquinil em proteìnas em eubacterias
BRPI0517381A (pt) componentes ortogonais de tradução para a incorporação in vivo de aminoácidos não naturais
ES2353814T3 (es) Proteinas n-glicosiladas recombinantes de celulas procariotas.
BRPI0411475A (pt) adições de código genético de aminoácido reativo não natural
NO20083491L (no) Metode for fremstilling av sulfonamid-substituerts alkoholer og mellomprodukter for slike
BRPI0519170A8 (pt) formulações de hormônio de crescimento humano que compreendem um aminoácido não naturalmente codificado
EA200801372A1 (ru) Способы получения замещенных пиримидинов
CL2011000671A1 (es) Procedimiento para formar una composicion acuosa de proteina de tejido muscular animal que contiene proteinas sarcoplasmicas y miofibrilares que comprende: a) triturar proteina muscular animal, b) mezclar dicha proteina con una composicion de una base de uso permitido en alimentos formado una solucion acuosa basica de ph 11,5-12,0.c) mezclar la solucion basica con una composicion acida formando una composicion acuosa de ph 4,7-11,0,d) triturar.
BRPI0519568A2 (pt) composiÇÕes de aminoacil-trna sintetase e seus usos
MX354100B (es) Formulaciones de proteinas y metodos para elaborarlas.
ATE542888T1 (de) Standortspezifische inkorporation nicht natürlicher aminosäuren in wirbeltierzellen
PT1991680E (pt) Sistema para a expressão de componentes ortogonais de tradução em células hospedeiras eubacterianas
CY1116506T1 (el) Αντισωματα εναντι masp-2
BRPI0519766A2 (pt) proteases Ácidas féngicas
MX2009002526A (es) Transcripcion de tarn supresor en celulas de vertebrados.
WO2004062592A3 (en) 2-o sulfatase compositions and related methods
AR113492A2 (es) Furina recombinante libre de proteínas sustancialmente animal y métodos para producirla
MY150226A (en) Novel h5 proteins, nucleic acid molecules and vectors encoding for those, and medicinal use
WO2007112082A3 (en) Methods and compositions for the identification of cancer markers
CY1109207T1 (el) Διαδικασια εχινοκανδινης
BRPI0518247A2 (pt) composiÇÕes compreendo inibidores de cinases jun n-terminais e uso
ATE478858T1 (de) Cyclische sulfone, die zur verwendung als bace- inhibitoren geeignet sind
CR9109A (es) Tratamiento de mastitis
EA200600439A1 (ru) 4- ((феноксиалкил)тио) феноксиуксусные кислоты и аналоги
ATE472316T1 (de) Haarbehandlungszusammensetzungen

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]