BRPI0515929A - método para modificar morfologia em uma planta, ácido nucléico, gene quimérico, dna recombinante, planta, célula vegetal, métodos para regular a expressão de um gene em uma planta, e para modificar o metabolismo dentro de uma célula de uma planta transgênica, e, usos de um ácido nucléico e de um gene quimérico - Google Patents

método para modificar morfologia em uma planta, ácido nucléico, gene quimérico, dna recombinante, planta, célula vegetal, métodos para regular a expressão de um gene em uma planta, e para modificar o metabolismo dentro de uma célula de uma planta transgênica, e, usos de um ácido nucléico e de um gene quimérico

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Publication number
BRPI0515929A
BRPI0515929A BRPI0515929-6A BRPI0515929A BRPI0515929A BR PI0515929 A BRPI0515929 A BR PI0515929A BR PI0515929 A BRPI0515929 A BR PI0515929A BR PI0515929 A BRPI0515929 A BR PI0515929A
Authority
BR
Brazil
Prior art keywords
plant
gene
nucleic acid
modifying
cell
Prior art date
Application number
BRPI0515929-6A
Other languages
English (en)
Inventor
Christopher John Robert Thomas
Original Assignee
Cambridge Advanced Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Cambridge Advanced Tech filed Critical Cambridge Advanced Tech
Publication of BRPI0515929A publication Critical patent/BRPI0515929A/pt
Publication of BRPI0515929B1 publication Critical patent/BRPI0515929B1/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8226—Stem-specific, e.g. including tubers, beets
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8222—Developmentally regulated expression systems, tissue, organ specific, temporal or spatial regulation
    • C12N15/8223—Vegetative tissue-specific promoters
    • C12N15/8229—Meristem-specific, e.g. nodal, apical
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8216—Methods for controlling, regulating or enhancing expression of transgenes in plant cells
    • C12N15/8237—Externally regulated expression systems
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
    • C12N15/8262—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield involving plant development
    • C12N15/8263—Ablation; Apoptosis
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
    • Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
    • Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
    • Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants

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  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Organic Chemistry (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Cell Biology (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)

Abstract

"MéTODO PARA MODIFICAR MORFOLOGIA EM UMA PLANTA, áCIDO NUCLEICO, GENE QUIMéRICO, DNA RECOMBINANTE, PLANTA, CéLULA VEGETAL, MéTODOS PARA REGULAR A EXPRESSãO DE UM GENE EM UMA PLANTA, E PARA MODIFICAR O METABOLISMO DENTRO DE UMA CéLULA DE UMA PLANTA TRANSGêNICA, E, USOS DE UM áCIDO NUCLEICO E DE UM GENE QUIMéRICO". A presente invenção se refere a um método para modificar morfologia em uma planta compreendendo introduzir em uma planta pelo menos um gene quimérico compreendendo uma seqüência promotora operativamente associada com uma seqüência de ácidos nucleicos, a seqüência promotora sendo operável para direcionar expressão em células específicas da planta,e a seqüência de ácidos nucleicos codificando pelo menos um produto de gene capaz de alterar o metabolismo de ou causar morte das células específicas e/ou células vizinhas. Em particular, a seqüência promotora é operável para direcionar expressão em gema lateral ou broto lateral e o ácido nucleico codificando pelo menos um produto de gene capaz de interromper o metabolismo de ou causar a morte da gema lateral ou broto lateral ou células vizinhas. Preferivelmente a seqüência promotora compreende a seqüência mostrada como SEQ ID No. 1 ou SEQ ID No. 7 ou SEQ ID No. 4, ou uma parte destas capaz de regular expressão de um gene, ou uma seqüência tendo pelo menos 60%, preferivelmente pelo menos 75%, de homologia com SEQ ID No. 1 ou SEQ ID No. 7 e sendo capaz de regular expressão de um gene.
BRPI0515929A 2004-09-29 2005-09-28 método para modificar morfologia em uma planta, ácido nucleico, gene quimérico, dna recombinante, métodos para regular a expressão de um gene em uma planta e para modificar o metabolismo de uma célula de uma planta transgênica, e, usos de um ácido nucleico e de um gene quimérico BRPI0515929B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0421598.4A GB0421598D0 (en) 2004-09-29 2004-09-29 Modification of plant development and morphology
PCT/GB2005/003719 WO2006035221A2 (en) 2004-09-29 2005-09-28 Modification of plant development and morphology

Publications (2)

Publication Number Publication Date
BRPI0515929A true BRPI0515929A (pt) 2008-08-12
BRPI0515929B1 BRPI0515929B1 (pt) 2017-05-02

Family

ID=33397428

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0515929A BRPI0515929B1 (pt) 2004-09-29 2005-09-28 método para modificar morfologia em uma planta, ácido nucleico, gene quimérico, dna recombinante, métodos para regular a expressão de um gene em uma planta e para modificar o metabolismo de uma célula de uma planta transgênica, e, usos de um ácido nucleico e de um gene quimérico

Country Status (9)

Country Link
US (3) US8093459B2 (pt)
EP (1) EP1794303B1 (pt)
CN (1) CN101048508B (pt)
AR (1) AR050809A1 (pt)
AT (1) ATE523594T1 (pt)
BR (1) BRPI0515929B1 (pt)
ES (1) ES2373318T3 (pt)
GB (1) GB0421598D0 (pt)
WO (1) WO2006035221A2 (pt)

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CN104109671B (zh) * 2014-07-10 2017-01-11 中国农业科学院作物科学研究所 一种蚜虫共生菌基因的dsRNA及其在抑制蚜虫生长中的应用
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CA3157607A1 (en) * 2019-11-26 2021-06-03 Changbao LI Methods of in planta transformation using axillary meristem
CN113774070B (zh) * 2020-08-21 2023-05-12 中国农业科学院烟草研究所 一种烟草打顶后抑制其侧枝生长的方法和材料
CN117153250B (zh) * 2023-10-31 2024-03-01 中日友好医院(中日友好临床医学研究所) 烟草依赖个体化干预基因变异位点筛选方法及试剂盒

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Also Published As

Publication number Publication date
US9481890B2 (en) 2016-11-01
AR050809A1 (es) 2006-11-22
US20090249518A1 (en) 2009-10-01
BRPI0515929B1 (pt) 2017-05-02
US20120011616A1 (en) 2012-01-12
WO2006035221A3 (en) 2006-05-18
EP1794303B1 (en) 2011-09-07
ES2373318T3 (es) 2012-02-02
CN101048508B (zh) 2012-05-16
US20140033364A1 (en) 2014-01-30
GB0421598D0 (en) 2004-10-27
WO2006035221A2 (en) 2006-04-06
HK1113390A1 (en) 2008-10-03
US8093459B2 (en) 2012-01-10
EP1794303A2 (en) 2007-06-13
CN101048508A (zh) 2007-10-03
US8575423B2 (en) 2013-11-05
ATE523594T1 (de) 2011-09-15

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