BRPI0517121A - célula hospedeira eucariótica de planta não-superior que contém proteìna de fusão recombinante dentro de conjuntos do tipo corpo de proteìna recombinante ( rpblas ), método para a produção de uma proteìna de fusão e molécula de ácido nucléico recombinante - Google Patents

célula hospedeira eucariótica de planta não-superior que contém proteìna de fusão recombinante dentro de conjuntos do tipo corpo de proteìna recombinante ( rpblas ), método para a produção de uma proteìna de fusão e molécula de ácido nucléico recombinante

Info

Publication number
BRPI0517121A
BRPI0517121A BRPI0517121-0A BRPI0517121A BRPI0517121A BR PI0517121 A BRPI0517121 A BR PI0517121A BR PI0517121 A BRPI0517121 A BR PI0517121A BR PI0517121 A BRPI0517121 A BR PI0517121A
Authority
BR
Brazil
Prior art keywords
recombinant
protein
fusion protein
cell containing
rblblas
Prior art date
Application number
BRPI0517121-0A
Other languages
English (en)
Inventor
Mugica Maria Dolores Ludevid
Miriam Bastida Virgili
Blanca Llompart Royo
Plabo Marzabal Luna
Quetglas Margarita Torrent
Original Assignee
Era Biotech Sa
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Era Biotech Sa filed Critical Era Biotech Sa
Publication of BRPI0517121A publication Critical patent/BRPI0517121A/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/415—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from plants
    • C07K14/425—Zeins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/475—Growth factors; Growth regulators
    • C07K14/485—Epidermal growth factor [EGF], i.e. urogastrone
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/585—Calcitonins
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/435—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from animals; from humans
    • C07K14/575—Hormones
    • C07K14/61—Growth hormone [GH], i.e. somatotropin
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K19/00—Hybrid peptides, i.e. peptides covalently bound to nucleic acids, or non-covalently bound protein-protein complexes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/02—Preparation of hybrid cells by fusion of two or more cells, e.g. protoplast fusion
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C12N15/62—DNA sequences coding for fusion proteins
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
    • C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
    • C12N15/8242—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits
    • C12N15/8257—Phenotypically and genetically modified plants via recombinant DNA technology with non-agronomic quality (output) traits, e.g. for industrial processing; Value added, non-agronomic traits for the production of primary gene products, e.g. pharmaceutical products, interferon
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K2319/00—Fusion polypeptide
    • C07K2319/01—Fusion polypeptide containing a localisation/targetting motif
    • C07K2319/04—Fusion polypeptide containing a localisation/targetting motif containing an ER retention signal such as a C-terminal HDEL motif

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Organic Chemistry (AREA)
  • Molecular Biology (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biotechnology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Toxicology (AREA)
  • Endocrinology (AREA)
  • Botany (AREA)
  • Cell Biology (AREA)
  • Pharmacology & Pharmacy (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Peptides Or Proteins (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

CéLULA HOSPEDEIRA EUCARIOTICA DE PLANTA NãO- SUPERIOR QUE CONTEM PROTEìNA DE FUSãO RECOMBINANTE DENTRO DE CONJUNTOS DO TIPO CORPO DE PROTEìNA RECOMBINANTE (RPBLAS), MéTODO PARA A PRODUçãO DE UMA PROTEìNA DE FUSãO E MOLéCULA DE áCIDO NUCLéICO RECOMBINANTE. Trata-se de um método para a formação de uma proteína de fusão que é expressa como um conjunto do tipo corpo de proteína recombinante em células hospedeiras eucarióticas e os organismos com exceção de plantas superiores como sistemas hospedeiros. Mais particularmente, os peptideos e as proteínas são fundidos às seqúências de proteínas que mediam a indução da formação de conjunto do tipo corpo de proteína recombinante (RPBLA), e são estavelmente expressos e acumulados nestas células hospedeiras após a transformação com um vetor apropriado. Também são apresentados métodos para a preparação da proteína de fusão.
BRPI0517121-0A 2004-11-29 2005-11-29 célula hospedeira eucariótica de planta não-superior que contém proteìna de fusão recombinante dentro de conjuntos do tipo corpo de proteìna recombinante ( rpblas ), método para a produção de uma proteìna de fusão e molécula de ácido nucléico recombinante BRPI0517121A (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
GBGB0426160.8A GB0426160D0 (en) 2004-11-29 2004-11-29 Production of proteins
PCT/EP2005/012877 WO2006056483A1 (en) 2004-11-29 2005-11-29 Production of proteins

Publications (1)

Publication Number Publication Date
BRPI0517121A true BRPI0517121A (pt) 2008-09-30

Family

ID=33561506

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0517121-0A BRPI0517121A (pt) 2004-11-29 2005-11-29 célula hospedeira eucariótica de planta não-superior que contém proteìna de fusão recombinante dentro de conjuntos do tipo corpo de proteìna recombinante ( rpblas ), método para a produção de uma proteìna de fusão e molécula de ácido nucléico recombinante

Country Status (12)

Country Link
US (1) US8822181B2 (pt)
EP (1) EP1819725B1 (pt)
JP (1) JP2008521391A (pt)
KR (1) KR101430081B1 (pt)
CN (1) CN101098886B (pt)
AR (1) AR051837A1 (pt)
AU (1) AU2005308921B2 (pt)
BR (1) BRPI0517121A (pt)
CA (1) CA2589164C (pt)
GB (1) GB0426160D0 (pt)
RU (1) RU2429243C2 (pt)
WO (1) WO2006056483A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426161D0 (en) * 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
US8163880B2 (en) 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
EP2356133B1 (en) 2008-10-10 2019-07-31 Zera Intein Protein Solutions, S.L. Recombinant protein bodies as immunogen-specific adjuvants
EP2418284A1 (en) * 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences
EP2468872A1 (en) * 2010-12-23 2012-06-27 Philip Morris Products S.A. Method for producing mullerian inhibitor substance in plants
CA2824152C (en) 2011-01-17 2022-12-13 Philip Morris Products S.A. Method and system for producing a heterologous polypeptide in a nicotiana tabacum plant
WO2012098111A1 (en) 2011-01-17 2012-07-26 Philip Morris Products S.A. Vectors for nucleic acid expression in plants
FI20115704A7 (fi) 2011-07-01 2013-01-02 Teknologian Tutkimuskeskus Vtt Oy Vierasproteiinien tuoton parantaminen
GB201514652D0 (en) * 2015-08-18 2015-09-30 Univ Cape Town Synthetic BTV VP2 multiepitope peptide vaccine
GB201514648D0 (en) * 2015-08-18 2015-09-30 Univ Cape Town Synthetic BTV VP2 fusion protein
CN109071611B (zh) * 2016-05-06 2022-11-01 葛兰素史克知识产权开发有限公司 产生重组蛋白的方法
US12679875B2 (en) * 2019-11-08 2026-07-14 Korea Institute Of Ocean Science & Technology Human epidermal growth factor-TF fusion protein and use thereof
CN112048518B (zh) * 2020-08-22 2022-06-24 江西农业大学 一种玉米醇溶蛋白降解酶的制备方法及其应用
US10894812B1 (en) 2020-09-30 2021-01-19 Alpine Roads, Inc. Recombinant milk proteins
WO2022072718A1 (en) 2020-09-30 2022-04-07 Nobell Foods, Inc. Recombinant milk proteins and food compositions comprising the same
US10947552B1 (en) 2020-09-30 2021-03-16 Alpine Roads, Inc. Recombinant fusion proteins for producing milk proteins in plants

Family Cites Families (12)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4215040A (en) 1978-12-22 1980-07-29 The Procter & Gamble Company Density separation process
US5650554A (en) 1991-02-22 1997-07-22 Sembiosys Genetics Inc. Oil-body proteins as carriers of high-value peptides in plants
DE69836552T3 (de) 1997-09-30 2014-10-09 The Regents Of The University Of California Herstellung von proteinen in pflanzensamen
US6489542B1 (en) * 1998-11-04 2002-12-03 Monsanto Technology Llc Methods for transforming plants to express Cry2Ab δ-endotoxins targeted to the plastids
AU2002256310A1 (en) 2001-04-18 2002-11-05 New Mexico State University Technology Transfer Corporation Co-expression of zein proteins
EP1521767B1 (en) * 2002-05-24 2009-12-16 Restoragen Inc. Methods and constructs for high yield expression of clostripain
ATE457355T1 (de) 2002-05-24 2010-02-15 Medtronic Inc Verfahren und dna-konstrukte zur produktion von polypeptiden mit hoher ausbeute
ES2224792B1 (es) * 2002-06-28 2007-02-16 Era Plantech, S.L. Produccion de peptidos y proteinas por acumulacion de cuerpos proteicos derivados de reticulos endoplasmico en plantas.
GB0426161D0 (en) 2004-11-29 2004-12-29 Era Plantech S L Protein isolation and purification
US8163880B2 (en) 2006-02-23 2012-04-24 Era Biotech S.A. Production of biologically active proteins
EP2356133B1 (en) 2008-10-10 2019-07-31 Zera Intein Protein Solutions, S.L. Recombinant protein bodies as immunogen-specific adjuvants
EP2418284A1 (en) 2010-08-13 2012-02-15 ERA Biotech, S.A. Protein body-inducing polypeptide sequences

Also Published As

Publication number Publication date
AU2005308921B2 (en) 2011-11-24
WO2006056483A1 (en) 2006-06-01
AU2005308921A1 (en) 2006-06-01
CA2589164A1 (en) 2006-06-01
CN101098886B (zh) 2012-09-26
RU2007124369A (ru) 2009-01-10
AR051837A1 (es) 2007-02-14
KR101430081B1 (ko) 2014-08-13
US20060121573A1 (en) 2006-06-08
US8822181B2 (en) 2014-09-02
EP1819725B1 (en) 2013-03-27
JP2008521391A (ja) 2008-06-26
GB0426160D0 (en) 2004-12-29
CA2589164C (en) 2014-10-28
RU2429243C2 (ru) 2011-09-20
KR20070091156A (ko) 2007-09-07
CN101098886A (zh) 2008-01-02
EP1819725A1 (en) 2007-08-22

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C07K 14/425 , C12N 15/62

Ipc: C12N 5/14 (2006.01), C12N 15/11 (2006.01), C12N 15

B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 5/14 , C12N 15/11 , C12N 15/62 , C12N 15/79

Ipc: C12N 15/63 (2006.01), C12N 15/29 (2006.01), C07K 1

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: C12N 15/63 , C12N 15/29 , C07K 14/415 , C07K 14/425

Ipc: C12N 5/14 (2006.01), C12N 5/16 (2006.01), C12N 15/

Ipc: C12N 5/14 (2006.01), C12N 5/16 (2006.01), C12N 15/

B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]