BRPI0607540A2 - ensaio para a produção de circovìrus porcino - Google Patents

ensaio para a produção de circovìrus porcino

Info

Publication number
BRPI0607540A2
BRPI0607540A2 BRPI0607540-1A BRPI0607540A BRPI0607540A2 BR PI0607540 A2 BRPI0607540 A2 BR PI0607540A2 BR PI0607540 A BRPI0607540 A BR PI0607540A BR PI0607540 A2 BRPI0607540 A2 BR PI0607540A2
Authority
BR
Brazil
Prior art keywords
cells
viral
supernatant
production
methods
Prior art date
Application number
BRPI0607540-1A
Other languages
English (en)
Inventor
Patrice Allibert
Lionel Pierre Cupillard
Jean Reyes
Original Assignee
Merial Ltd
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Merial Ltd filed Critical Merial Ltd
Publication of BRPI0607540A2 publication Critical patent/BRPI0607540A2/pt
Publication of BRPI0607540B1 publication Critical patent/BRPI0607540B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • C12N7/02—Recovery or purification
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/569—Immunoassay; Biospecific binding assay; Materials therefor for microorganisms, e.g. protozoa, bacteria, viruses
    • G01N33/56983—Viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N7/00—Viruses; Bacteriophages; Compositions thereof; Preparation or purification thereof
    • A—HUMAN NECESSITIES
    • A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
    • A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
    • A61K39/00—Medicinal preparations containing antigens or antibodies
    • A61K2039/51—Medicinal preparations containing antigens or antibodies comprising whole cells, viruses or DNA/RNA
    • A61K2039/525—Virus
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N2750/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssDNA viruses
    • C12N2750/00011—Details
    • C12N2750/10011—Circoviridae
    • C12N2750/10051—Methods of production or purification of viral material
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N2333/00—Assays involving biological materials from specific organisms or of a specific nature
    • G01N2333/005—Assays involving biological materials from specific organisms or of a specific nature from viruses
    • G01N2333/01—DNA viruses

Landscapes

  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Virology (AREA)
  • Biomedical Technology (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Genetics & Genomics (AREA)
  • Zoology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Medicinal Chemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Physics & Mathematics (AREA)
  • Analytical Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Medicines Containing Material From Animals Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Compositions Of Macromolecular Compounds (AREA)

Abstract

ENSAIO PARA A PRODUçãO DE CIRCOVìRUS PORCINO. A presente invenção refere-se a métodos para a determinação do título viral de uma cultura de células hospedeiras de animal infectadas com um circovírus. Os métodos baseados em FACS da invenção podem incluir a determinação da viabilidade das células hospedeiras no sobrenadante em um sobrenadante de meio de cultura celular e daquelas células que permanecem aderidas a um suporte sólido. Detectar e medir o percentual de células que expressam os antígenos virais de ORF1 e ORF2 pode determinar a carga viral das células hospedeiras cultivadas. O rendimento do vírus pode ser estabelecido pela detecção e medida de ambos os antígenos nas células sobrenadantes, por exemplo, 5 a 7 dias a partir de quando as células hospedeiras são transferidas para um meio livre de soro. Os métodos da invenção podem fornecer dados quantitativos rápidos. Isso permite o monitoramento em andamento repetido da produção viral através do período de incubação e pronta seleção do ponto de coleta mais apropriado.
BRPI0607540A 2005-04-13 2006-04-13 ensaio para a produção de circovírus porcino BRPI0607540B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US67089205P 2005-04-13 2005-04-13
PCT/US2006/014068 WO2006113435A2 (en) 2005-04-13 2006-04-13 Method for porcine circovirus production and assays for monitoring production

Publications (2)

Publication Number Publication Date
BRPI0607540A2 true BRPI0607540A2 (pt) 2009-09-15
BRPI0607540B1 BRPI0607540B1 (pt) 2017-05-09

Family

ID=37115731

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0607540A BRPI0607540B1 (pt) 2005-04-13 2006-04-13 ensaio para a produção de circovírus porcino

Country Status (20)

Country Link
US (2) US7358075B2 (pt)
EP (2) EP1869471B1 (pt)
JP (1) JP5408998B2 (pt)
KR (1) KR101369656B1 (pt)
CN (1) CN101194165B (pt)
BR (1) BRPI0607540B1 (pt)
CA (1) CA2604691C (pt)
CR (1) CR9511A (pt)
CY (1) CY1117850T1 (pt)
DK (1) DK1869471T3 (pt)
ES (1) ES2589928T3 (pt)
HR (1) HRP20161132T1 (pt)
HU (1) HUE028343T2 (pt)
ME (1) ME02683B (pt)
MX (1) MX2007012786A (pt)
PL (1) PL1869471T3 (pt)
PT (1) PT1869471T (pt)
RS (1) RS55085B1 (pt)
SI (1) SI1869471T1 (pt)
WO (1) WO2006113435A2 (pt)

Families Citing this family (25)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
FR2772047B1 (fr) * 1997-12-05 2004-04-09 Ct Nat D Etudes Veterinaires E Sequence genomique et polypeptides de circovirus associe a la maladie de l'amaigrissement du porcelet (map), applications au diagnostic et a la prevention et/ou au traitement de l'infection
US7833707B2 (en) 2004-12-30 2010-11-16 Boehringer Ingelheim Vetmedica, Inc. Methods of overexpression and recovery of porcine circovirus type 2 ORF2
US7700285B1 (en) * 2005-12-29 2010-04-20 Boehringer Ingelheim Vetmedica, Inc. PCV2 immunogenic compositions and methods of producing such compositions
UA95602C2 (ru) 2004-12-30 2011-08-25 Берингер Ингельхейм Ветмедика, Инк. Иммуногенная композиция цвс2 и способы приготовления такой композиции
US20080226594A1 (en) * 2005-02-03 2008-09-18 Hans Nauwynck Culturing circular ssdna viruses for the production of vaccines
CA2635430A1 (en) 2005-12-29 2007-08-23 Boehringer Ingelheim Vetmedica, Inc. Use of a pcv2 immunogenic composition for lessening clinical symptoms in pigs
DK2275132T3 (da) 2005-12-29 2021-05-17 Boehringer Ingelheim Animal Health Usa Inc Multivalente pcv2-immunogeniske sammensætninger og fremgangsmåder til at fremstille dem
WO2008073464A2 (en) * 2006-12-11 2008-06-19 Boehringer Ingelheim Vetmedica, Inc. Effective method of treatment of porcine circovirus and lawsonia intracellularis infections
PT2094872T (pt) * 2006-12-15 2017-04-27 Boehringer Ingelheim Vetmedica Inc Tratamento de porcos seropositivos para o anticorpo anti-pcv2 com antigénio de pcv2
EP1941903A1 (en) 2007-01-03 2008-07-09 Boehringer Ingelheim Vetmedica Gmbh Prophylaxis and treatment of PRDC
EP1958644A1 (en) 2007-02-13 2008-08-20 Boehringer Ingelheim Vetmedica Gmbh Prevention and treatment of sub-clinical pcvd
US8537285B2 (en) * 2007-03-14 2013-09-17 Larry Silver Carrier recovery system with phase noise suppression
CN101688185B (zh) 2007-05-11 2013-05-22 淡马锡生命科学研究院有限公司 对2型猪环状构象病毒(pcv2)感染高度容许的均质细胞系的产生
US7829274B2 (en) * 2007-09-04 2010-11-09 Boehringer Ingelheim Vetmedica, Inc. Reduction of concomitant infections in pigs by the use of PCV2 antigen
CN101932336B (zh) * 2007-12-31 2014-07-09 贝林格尔.英格海姆维特梅迪卡有限公司 有外来氨基酸插入的pcv2 orf2病毒样颗粒
KR20100113582A (ko) 2008-01-23 2010-10-21 베링거잉겔하임베트메디카인코퍼레이티드 Ｐｃｖ2 마이코플라즈마 히오뉴모니에 면역원성 조성물 및 당해 조성물의 생산 방법
TWI627281B (zh) 2009-09-02 2018-06-21 百靈佳殷格翰家畜藥品公司 降低ｐｃｖ－２組合物殺病毒活性之方法及具有改良免疫原性之ｐｃｖ－２組合物
CN101773667A (zh) * 2010-01-28 2010-07-14 洛阳普莱柯生物工程有限公司 一种猪圆环病毒ii型疫苗制备
CN102690790B (zh) * 2012-04-27 2013-09-25 成都天邦生物制品有限公司 猪圆环病毒2型细胞悬浮培养生产方法
EP2994162B1 (en) 2013-05-08 2021-04-07 Pharmgate Biologics Inc. Vaccine for pcv2 and mycoplasma
CA2925281C (en) 2013-09-25 2022-05-03 Zoetis Services Llc Pcv2b divergent vaccine composition and methods of use
ES2778425T3 (es) 2013-10-02 2020-08-10 Boehringer Ingelheim Animal Health Usa Inc Variante de la proteína ORF2 del PCV2 y partículas similares a virus compuestas por esta
ES2837133T3 (es) 2015-07-24 2021-06-29 Boehringer Ingelheim Animal Health Usa Inc Procedimiento de cuantificación de virus de alto rendimiento
DE102018100967B4 (de) 2018-01-17 2019-08-14 Immatics US, Inc. Verfahren zur feststellung der wirksamkeit von viralen vektoren
DE102018010282A1 (de) 2018-01-17 2019-07-18 Immatics US, Inc. Verfahren zur Feststellung der Wirksamkeit von viralen Vektoren

Family Cites Families (7)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US6391314B1 (en) * 1997-10-03 2002-05-21 Merial Porcine circoviruses vaccines diagnostic reagents
UA78180C2 (uk) * 1997-10-03 2007-03-15 Меріаль Кільцевий вірус свині типу ii, вакцини та діагностичні реагенти
EP1111040A1 (en) * 1999-12-10 2001-06-27 Daniel Favre Infection of eukaryotic cells with viruses in vitro
WO2001096377A2 (en) * 2000-06-15 2001-12-20 Purdue Research Foundation Vaccine for congenital tremors in pigs
DE10044648A1 (de) * 2000-09-08 2002-03-21 Aventis Behring Gmbh Verfahren zur Vermehrung oder zur Entfernung von Cirocoviren aus biologischem Material
DK1520008T3 (da) * 2002-07-09 2012-10-15 Baxter Int Medium frit for dyreprotein til dyrkning af celler
CA2807534A1 (en) * 2003-02-25 2005-02-17 Medimmune Vaccines, Inc. Methods of producing influenza vaccine compositions

Also Published As

Publication number Publication date
DK1869471T3 (en) 2016-08-01
PL1869471T3 (pl) 2017-02-28
CN101194165A (zh) 2008-06-04
US20060246425A1 (en) 2006-11-02
CA2604691C (en) 2013-12-31
WO2006113435A2 (en) 2006-10-26
JP5408998B2 (ja) 2014-02-05
KR101369656B1 (ko) 2014-03-04
KR20070120603A (ko) 2007-12-24
EP3088893B1 (en) 2018-02-14
HUE028343T2 (en) 2016-12-28
CR9511A (es) 2008-01-29
BRPI0607540B1 (pt) 2017-05-09
CA2604691A1 (en) 2006-10-26
US7358075B2 (en) 2008-04-15
HRP20161132T1 (hr) 2016-12-02
EP1869471A2 (en) 2007-12-26
US7476507B2 (en) 2009-01-13
ES2589928T3 (es) 2016-11-17
WO2006113435A3 (en) 2007-06-21
HK1121232A1 (en) 2009-04-17
JP2008536499A (ja) 2008-09-11
ME02683B (me) 2017-06-20
CY1117850T1 (el) 2017-05-17
EP3088893A1 (en) 2016-11-02
MX2007012786A (es) 2008-01-11
CN101194165B (zh) 2013-01-09
SI1869471T1 (sl) 2016-09-30
RS55085B1 (sr) 2016-12-30
PT1869471T (pt) 2016-08-16
US20080241821A1 (en) 2008-10-02
EP1869471B1 (en) 2016-06-08

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B25A Requested transfer of rights approved

Owner name: MERIAL, INC. (US)

B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]
B16C Correction of notification of the grant [chapter 16.3 patent gazette]
B25A Requested transfer of rights approved