BRPI0608103A2 - dispositivo para detecção e identificação rápida de microorganismos isolados sem cultura preliminar - Google Patents

dispositivo para detecção e identificação rápida de microorganismos isolados sem cultura preliminar

Info

Publication number
BRPI0608103A2
BRPI0608103A2 BRPI0608103-7A BRPI0608103A BRPI0608103A2 BR PI0608103 A2 BRPI0608103 A2 BR PI0608103A2 BR PI0608103 A BRPI0608103 A BR PI0608103A BR PI0608103 A2 BRPI0608103 A2 BR PI0608103A2
Authority
BR
Brazil
Prior art keywords
microchannels
microchannel
microorganisms
filter
identification
Prior art date
Application number
BRPI0608103-7A
Other languages
English (en)
Inventor
Sergey Gazenko
Original Assignee
Nanologix Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Nanologix Inc filed Critical Nanologix Inc
Publication of BRPI0608103A2 publication Critical patent/BRPI0608103A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/02—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving viable microorganisms
    • C12Q1/04—Determining presence or kind of microorganism; Use of selective media for testing antibiotics or bacteriocides; Compositions containing a chemical indicator therefor

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Toxicology (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biotechnology (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

DISPOSITIVO PARA DETECçãO E IDENTIFICAçãO RáPIDA DE MICROORGANISMOS ISOLADOS SEM CULTURA PRELIMINAR. Esta invenção descreve um dispositivo que compreende uma placa de microcanais, filtro e suporte poroso para o filtro, que durante o desempenho do método é substituído por um bloco de ágar puro, e elementos estruturais de apoio. O dispositivo destina-se à detecção e/ou identificação rápida de microorganismos. Os microorganismos são retidos pela filtração em microcanais longos (diâmetro/comprimento= 1/10 - 1/100), cilíndricos, paralelos, abertos em ambos os lados e com uma das extremidades presa a um filtro. Uma placa de microcanais abriga diversos microcanais (canal com 1 - 3Opm de diâmetro possível, 100 - 1000<109>m de comprimento e um número de microcanais por cm^ 2^ = 100.000 - 1.000.000). A placa de microcanais com as células retidas na superfície do filtro é fixada a um bloco de ágar impregnado por substrato(s) artificial(is), de forma que as moléculas dos substratos artificiais encherão todos os microcanais. As células retidas produzirão moléculas coloridas ou fluorescentes dos substratos artificiais. Estas moléculas são recolhidas no volume muito pequeno de um microcanal. O volume extremamente reduzido de um microcanal (1/25 milionésima parte de um mililitro) permite recolher uma concentração detectável de substâncias coloridas ou fluorescentes em um período de tempo bastante curto (vários minutos), O método da enzima-substrato artificial é capaz de detectar até mesmo uma única célula de uma amostra filtrada e/ou identificá-la pelo imunoensaio de enzima.
BRPI0608103-7A 2005-04-20 2006-04-20 dispositivo para detecção e identificação rápida de microorganismos isolados sem cultura preliminar BRPI0608103A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US11/109,857 US8663909B2 (en) 2002-05-09 2005-04-20 Device for rapid detection and identification of single microorganisms without preliminary growth
PCT/US2006/015321 WO2006113930A2 (en) 2005-04-20 2006-04-20 Device for rapid detection and identification of single microoganisms without preliminary growth

Publications (1)

Publication Number Publication Date
BRPI0608103A2 true BRPI0608103A2 (pt) 2009-11-03

Family

ID=37115982

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0608103-7A BRPI0608103A2 (pt) 2005-04-20 2006-04-20 dispositivo para detecção e identificação rápida de microorganismos isolados sem cultura preliminar

Country Status (5)

Country Link
US (1) US8663909B2 (pt)
EP (1) EP1871892A4 (pt)
CN (1) CN101203617B (pt)
BR (1) BRPI0608103A2 (pt)
WO (1) WO2006113930A2 (pt)

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Also Published As

Publication number Publication date
CN101203617B (zh) 2012-03-14
EP1871892A2 (en) 2008-01-02
WO2006113930A3 (en) 2007-08-09
CN101203617A (zh) 2008-06-18
EP1871892A4 (en) 2009-08-12
WO2006113930A2 (en) 2006-10-26
US20050221403A1 (en) 2005-10-06
US8663909B2 (en) 2014-03-04

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Technical examination (opinion): publication of technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: INDEFIRO O PEDIDO DE ACORDO COM O(S) ARTIGO(S) 8O C/C 11, 8O C/C 13 E 25 DA LPI.

B09B Patent application refused [chapter 9.2 patent gazette]

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B15K Others concerning applications: alteration of classification

Free format text: PROCEDIMENTO AUTOMATICO DE RECLASSIFICACAO. AS CLASSIFICACOES IPC ANTERIORES ERAM: C12Q 1/00; C12Q 1/68; G01N 1/00; G01N 1/18; G01N 15/06; G01N 21/00; G01N 21/75; G01N 33/53; G01N 33/566; C12M 1/34; C12M 1/36; C12M 1/38; C12M 3/00; B01L 11/00; B32B 5/02.

Ipc: C12Q 1/04 (2006.01)

Ipc: C12Q 1/04 (2006.01)