BRPI0701529A2 - nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit - Google Patents

nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit

Info

Publication number
BRPI0701529A2
BRPI0701529A2 BRPI0701529-1A BRPI0701529A BRPI0701529A2 BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2 BR PI0701529 A BRPI0701529 A BR PI0701529A BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2
Authority
BR
Brazil
Prior art keywords
nucleotide sequences
kit
detection
quantification
quantifying
Prior art date
Application number
BRPI0701529-1A
Other languages
Portuguese (pt)
Inventor
Eraldo Luiz Batista Jr
Santos Diogenes Santiago
Jocelei Maria Chies
Luiz Augusto Basso
Original Assignee
Uniao Brasileira De Educacao E Assistencia Ubea
Quatro G Pesquisa & Desenvolvimento
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniao Brasileira De Educacao E Assistencia Ubea, Quatro G Pesquisa & Desenvolvimento filed Critical Uniao Brasileira De Educacao E Assistencia Ubea
Priority to BRPI0701529-1A priority Critical patent/BRPI0701529A2/en
Priority to PCT/BR2008/000091 priority patent/WO2008122097A1/en
Publication of BRPI0701529A2 publication Critical patent/BRPI0701529A2/en

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Padrão de Referência Contendo Sequência Nucleotídica, Processo para a Detecção e/ou Quantificação de Seqúências Nucleotídicas, e Kit. A presente invenção proporciona meios práticos e eficazes pelos quais sequências nucleotídicas de qualquer organismo podem ser utilizadas como padrões em sistemas de detecção e/ou quantificação, notadamente nos casos em que são empregadas técnicas de amplificação de ácidos nucléicos. Os padrões de referência da invenção são úteis na quantificação de seqúências nucleotidicas e/ou de organismos em tecidos, fluidos biológicos, alimentos e cosméticos, a partir do emprego da técnica de Reação de Polimerase em Cadeia, preferencialmente associada à fluorescência, ou PCR em Tempo Real. As aplicações da invenção incluem a geração de dados em pesquisa que envolva quantificação de ácidos nucléicos, incluindo DNA e/ou RNA, bem como dos organismos que os contêm, como patógenos virais ou bacterianos, sendo, portanto, útil em serviços de diagnóstico hospitalar para detecção e quantificação de patógenos em quadros de infecção e na indústria alimentícia e de cosméticos. A presente invenção também proporciona um melhorado processo para a detecção e/ou quantificação de seqúências nucleotídicas, e um kit.Reference Standard Containing Nucleotide Sequence, Process for Detecting and / or Quantifying Nucleotide Sequences, and Kit. The present invention provides practical and effective means by which nucleotide sequences from any organism can be used as standards in detection and / or quantification systems. , especially where nucleic acid amplification techniques are employed. The reference standards of the invention are useful in quantifying nucleotide sequences and / or organisms in tissues, biological fluids, food and cosmetics by employing the polymerase chain reaction, preferably fluorescence, or Time PCR technique. Real. Applications of the invention include research data generation involving quantification of nucleic acids, including DNA and / or RNA, as well as the organisms containing them, such as viral or bacterial pathogens, and is therefore useful in hospital diagnostic services for detection and quantification of pathogens in infection and in the food and cosmetics industry. The present invention also provides an improved method for detecting and / or quantifying nucleotide sequences, and a kit.

BRPI0701529-1A 2007-04-05 2007-04-05 nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit BRPI0701529A2 (en)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BRPI0701529-1A BRPI0701529A2 (en) 2007-04-05 2007-04-05 nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit
PCT/BR2008/000091 WO2008122097A1 (en) 2007-04-05 2008-04-04 Synthesis of standards for detection and quantification of nucleic acids and kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0701529-1A BRPI0701529A2 (en) 2007-04-05 2007-04-05 nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit

Publications (1)

Publication Number Publication Date
BRPI0701529A2 true BRPI0701529A2 (en) 2008-11-18

Family

ID=39830422

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0701529-1A BRPI0701529A2 (en) 2007-04-05 2007-04-05 nucleotide sequence reference standard, process for detection and / or quantitation of nucleotide sequences, and kit

Country Status (2)

Country Link
BR (1) BRPI0701529A2 (en)
WO (1) WO2008122097A1 (en)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201720088D0 (en) 2017-12-01 2018-01-17 Univ Oslo Hf PCR Controls
CN110408612B (en) * 2019-07-09 2023-02-21 上海市计量测试技术研究院(中国上海测试中心、华东国家计量测试中心、上海市计量器具强制检定中心) Protective agent for low-concentration DNA standard substance, preservation method and application
CN112680534B (en) * 2021-01-21 2022-10-28 哈尔滨医科大学 Mycobacterium tuberculosis sRNA fluorescent quantitative PCR standard substance for identifying false positive reaction and application thereof
CN117867086B (en) * 2024-03-12 2024-06-25 北京雅康博生物科技有限公司 Standard substance for quantitative high-throughput sequencing library and preparation method and application thereof
CN120485408B (en) * 2025-07-17 2025-10-28 中国食品发酵工业研究院有限公司 LNA-qPCR accurate detection method of bifidobacterium longum subspecies infancy CICC 6069, and primers, probes, kit and application thereof

Also Published As

Publication number Publication date
WO2008122097A1 (en) 2008-10-16

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Legal Events

Date Code Title Description
B03A Publication of an application: publication of a patent application or of a certificate of addition of invention
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B07D Technical examination (opinion) related to article 229 of industrial property law
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa)
B08F Application fees: dismissal - article 86 of industrial property law
B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)