BRPI0701529A2 - padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit - Google Patents

padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit

Info

Publication number
BRPI0701529A2
BRPI0701529A2 BRPI0701529-1A BRPI0701529A BRPI0701529A2 BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2 BR PI0701529 A BRPI0701529 A BR PI0701529A BR PI0701529 A2 BRPI0701529 A2 BR PI0701529A2
Authority
BR
Brazil
Prior art keywords
nucleotide sequences
kit
detection
quantification
quantifying
Prior art date
Application number
BRPI0701529-1A
Other languages
English (en)
Inventor
Eraldo Luiz Batista Jr
Santos Diogenes Santiago
Jocelei Maria Chies
Luiz Augusto Basso
Original Assignee
Uniao Brasileira De Educacao E Assistencia Ubea
Quatro G Pesquisa & Desenvolvimento
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Uniao Brasileira De Educacao E Assistencia Ubea, Quatro G Pesquisa & Desenvolvimento filed Critical Uniao Brasileira De Educacao E Assistencia Ubea
Priority to BRPI0701529-1A priority Critical patent/BRPI0701529A2/pt
Priority to PCT/BR2008/000091 priority patent/WO2008122097A1/en
Publication of BRPI0701529A2 publication Critical patent/BRPI0701529A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/166—Oligonucleotides used as internal standards, controls or normalisation probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Immunology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

Padrão de Referência Contendo Sequência Nucleotídica, Processo para a Detecção e/ou Quantificação de Seqúências Nucleotídicas, e Kit. A presente invenção proporciona meios práticos e eficazes pelos quais sequências nucleotídicas de qualquer organismo podem ser utilizadas como padrões em sistemas de detecção e/ou quantificação, notadamente nos casos em que são empregadas técnicas de amplificação de ácidos nucléicos. Os padrões de referência da invenção são úteis na quantificação de seqúências nucleotidicas e/ou de organismos em tecidos, fluidos biológicos, alimentos e cosméticos, a partir do emprego da técnica de Reação de Polimerase em Cadeia, preferencialmente associada à fluorescência, ou PCR em Tempo Real. As aplicações da invenção incluem a geração de dados em pesquisa que envolva quantificação de ácidos nucléicos, incluindo DNA e/ou RNA, bem como dos organismos que os contêm, como patógenos virais ou bacterianos, sendo, portanto, útil em serviços de diagnóstico hospitalar para detecção e quantificação de patógenos em quadros de infecção e na indústria alimentícia e de cosméticos. A presente invenção também proporciona um melhorado processo para a detecção e/ou quantificação de seqúências nucleotídicas, e um kit.
BRPI0701529-1A 2007-04-05 2007-04-05 padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit BRPI0701529A2 (pt)

Priority Applications (2)

Application Number Priority Date Filing Date Title
BRPI0701529-1A BRPI0701529A2 (pt) 2007-04-05 2007-04-05 padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit
PCT/BR2008/000091 WO2008122097A1 (en) 2007-04-05 2008-04-04 Synthesis of standards for detection and quantification of nucleic acids and kit

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI0701529-1A BRPI0701529A2 (pt) 2007-04-05 2007-04-05 padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit

Publications (1)

Publication Number Publication Date
BRPI0701529A2 true BRPI0701529A2 (pt) 2008-11-18

Family

ID=39830422

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0701529-1A BRPI0701529A2 (pt) 2007-04-05 2007-04-05 padrço de referÊncia contendo seqÜÊncia nucleotÍdica, processo para a detecÇço e/ou quantificaÇço de seqÜÊncias nucleotÍdicas, e kit

Country Status (2)

Country Link
BR (1) BRPI0701529A2 (pt)
WO (1) WO2008122097A1 (pt)

Families Citing this family (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
GB201720088D0 (en) 2017-12-01 2018-01-17 Univ Oslo Hf PCR Controls
CN110408612B (zh) * 2019-07-09 2023-02-21 上海市计量测试技术研究院(中国上海测试中心、华东国家计量测试中心、上海市计量器具强制检定中心) 一种低浓度dna标准物质的保护剂、保存方法及应用
CN112680534B (zh) * 2021-01-21 2022-10-28 哈尔滨医科大学 一种用于识别假阳性反应的结核分枝杆菌sRNA荧光定量PCR标准品及其应用
CN117867086B (zh) * 2024-03-12 2024-06-25 北京雅康博生物科技有限公司 高通量测序文库定量用标准品及其制备方法和应用
CN120485408B (zh) * 2025-07-17 2025-10-28 中国食品发酵工业研究院有限公司 长双歧杆菌婴儿亚种CICC 6069的LNA-qPCR精准检测方法及其引物、探针、试剂盒与应用

Also Published As

Publication number Publication date
WO2008122097A1 (en) 2008-10-16

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Legal Events

Date Code Title Description
B03A Publication of an application: publication of a patent application or of a certificate of addition of invention
B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B07D Technical examination (opinion) related to article 229 of industrial property law
B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa)
B08F Application fees: dismissal - article 86 of industrial property law
B08K Lapse as no evidence of payment of the annual fee has been furnished to inpi (acc. art. 87)