BRPI0705744A2 - composição de enzimas, uso da composição na hidrólise enzimática de material lignocelulósico, processo de produção de enzimas que degradam a fração de polissacarìdeos da biomassa, processo de produção de álcool utilizando a composição de enzima - Google Patents
composição de enzimas, uso da composição na hidrólise enzimática de material lignocelulósico, processo de produção de enzimas que degradam a fração de polissacarìdeos da biomassa, processo de produção de álcool utilizando a composição de enzimaInfo
- Publication number
- BRPI0705744A2 BRPI0705744A2 BRPI0705744-0A BRPI0705744A BRPI0705744A2 BR PI0705744 A2 BRPI0705744 A2 BR PI0705744A2 BR PI0705744 A BRPI0705744 A BR PI0705744A BR PI0705744 A2 BRPI0705744 A2 BR PI0705744A2
- Authority
- BR
- Brazil
- Prior art keywords
- enzymes
- composition
- biomass
- cellulolytic
- enzyme composition
- Prior art date
Links
- 108090000790 Enzymes Proteins 0.000 title abstract 23
- 102000004190 Enzymes Human genes 0.000 title abstract 23
- 239000002028 Biomass Substances 0.000 title abstract 4
- LFQSCWFLJHTTHZ-UHFFFAOYSA-N Ethanol Chemical compound CCO LFQSCWFLJHTTHZ-UHFFFAOYSA-N 0.000 title abstract 4
- 238000004519 manufacturing process Methods 0.000 title abstract 4
- 150000004676 glycans Chemical class 0.000 title abstract 3
- 229920001282 polysaccharide Polymers 0.000 title abstract 3
- 239000005017 polysaccharide Substances 0.000 title abstract 3
- 230000007071 enzymatic hydrolysis Effects 0.000 title 1
- 238000006047 enzymatic hydrolysis reaction Methods 0.000 title 1
- 239000012978 lignocellulosic material Substances 0.000 title 1
- 230000001461 cytolytic effect Effects 0.000 abstract 5
- 238000000855 fermentation Methods 0.000 abstract 4
- 230000004151 fermentation Effects 0.000 abstract 4
- 241000894007 species Species 0.000 abstract 4
- 241000499912 Trichoderma reesei Species 0.000 abstract 3
- 230000002573 hemicellulolytic effect Effects 0.000 abstract 3
- 238000000034 method Methods 0.000 abstract 3
- 101000874334 Dalbergia nigrescens Isoflavonoid 7-O-beta-apiosyl-glucoside beta-glycosidase Proteins 0.000 abstract 2
- 101000757733 Enterococcus faecalis (strain ATCC 700802 / V583) Autolysin Proteins 0.000 abstract 2
- 229920002488 Hemicellulose Polymers 0.000 abstract 2
- 101000757734 Mycolicibacterium phlei 38 kDa autolysin Proteins 0.000 abstract 2
- 235000020054 awamori Nutrition 0.000 abstract 2
- 230000000694 effects Effects 0.000 abstract 2
- 230000002255 enzymatic effect Effects 0.000 abstract 2
- 229920005610 lignin Polymers 0.000 abstract 2
- 230000002195 synergetic effect Effects 0.000 abstract 2
- 241001513093 Aspergillus awamori Species 0.000 abstract 1
- 108010085318 carboxymethylcellulase Proteins 0.000 abstract 1
- 239000012228 culture supernatant Substances 0.000 abstract 1
- 230000007062 hydrolysis Effects 0.000 abstract 1
- 238000006460 hydrolysis reaction Methods 0.000 abstract 1
- 239000000463 material Substances 0.000 abstract 1
- 102000004196 processed proteins & peptides Human genes 0.000 abstract 1
- 108090000765 processed proteins & peptides Proteins 0.000 abstract 1
Classifications
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01021—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2437—Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
- C12N9/14—Hydrolases (3)
- C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
- C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
- C12N9/2405—Glucanases
- C12N9/2434—Glucanases acting on beta-1,4-glucosidic bonds
- C12N9/2445—Beta-glucosidase (3.2.1.21)
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y301/00—Hydrolases acting on ester bonds (3.1)
- C12Y301/01—Carboxylic ester hydrolases (3.1.1)
- C12Y301/01073—Feruloyl esterase (3.1.1.73)
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- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01004—Cellulase (3.2.1.4), i.e. endo-1,4-beta-glucanase
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Y—ENZYMES
- C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
- C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
- C12Y302/01008—Endo-1,4-beta-xylanase (3.2.1.8)
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
- Y02E50/00—Technologies for the production of fuel of non-fossil origin
- Y02E50/10—Biofuels, e.g. bio-diesel
Landscapes
- Chemical & Material Sciences (AREA)
- Organic Chemistry (AREA)
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Wood Science & Technology (AREA)
- Zoology (AREA)
- Bioinformatics & Cheminformatics (AREA)
- Engineering & Computer Science (AREA)
- Genetics & Genomics (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Engineering & Computer Science (AREA)
- Medicinal Chemistry (AREA)
- Molecular Biology (AREA)
- Biomedical Technology (AREA)
- Biotechnology (AREA)
- Microbiology (AREA)
- Enzymes And Modification Thereof (AREA)
- Micro-Organisms Or Cultivation Processes Thereof (AREA)
- Preparation Of Compounds By Using Micro-Organisms (AREA)
Abstract
COMPOSIçãO DE ENZIMAS, USO DA COMPOSIçãO NA HIDRóLISE ENZIMáTICA DE MATERIAL LIGNOCELULÈSICO, PROCESSO DE PRODUçãO DE ENZIMAS QUE DEGRADAM A FRAçãO DE POLISSAC.ARìDEOS DA BIOMASSA, PROCESSO DE PRODUçãO DE áLCOOL UTILIZANDO A COMPOSIçãO DE ENZIMAS. A presente invenção se refere a uma composição de enzimas compreendendo (a) enzimas celulolíticas obtidas por fermentação com Trichoderrna reesei; (b) enzimas celulolíticas, hemicelulolíticas, <225>-glicosidase e enzimas acessórias obtidas por fermentação com Aspergilius awamori; (c) adicionalmente, pelo menos uma espécie molecular selecionada do grupo de enzimas e/ou peptideos com atividade CMCase, ditas espécies moleculares estando presentes nos sobrenadantes do cultivo de T. reesei e/ou A. awamori como apresentado no zimograma da Figura 6; e (d) opcionalmente, um veículo compatível com ditas enzimas, sendo que as ditas enzimas (a) e (b) interagem de modo a resultar em efeito sinérgico dado pela ação das enzimas acessórias que, entre outras atividades, rompem as ligações entre a lignina e os polissacarídeos da biomassa, notadamente a hemicelulose. A presente invenção compreende, ainda, uma composição de enzimas compreendendo: (a) enzimas celuloliticas obtidas por fermentação com Trichoderma .reesei; (b) enzimas celuloliticas, hemicelulolíticas, <225>-glicosidase e enzimas acessórias obtidas por fermentação com Aspergilius awamori; e (c) opcionalmente um veículo compatível com ditas enzimas, sendo que as ditas enzimas (a) e (b) interagem de modo a resultar em efeito sínérgíco dado pela ação das enzimas acessórias e espécies moleculares que atuam de forma diferenciada nos polissacarideos da biomassa e também nas ligações entre a lignina e a hemicelulose. A invenção ainda inclui um processo de produção de ditas enzimas celuloliticas e hemícelulolítícas, <225>- glicosidase e enzimas acessórias e ditas espécies moleculares que compõem a composição da invenção e um processo de produção de álcool a partir de biomassa utilizando, na etapa de hidrólise enzímática, a composição da invenção.
Priority Applications (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0705744-0A BRPI0705744B1 (pt) | 2007-11-19 | 2007-11-19 | Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition |
| PCT/BR2008/000344 WO2009065199A1 (en) | 2007-11-19 | 2008-11-19 | Enzyme composition from trichoderma reesei and aspergillus awamori |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0705744-0A BRPI0705744B1 (pt) | 2007-11-19 | 2007-11-19 | Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition |
Publications (2)
| Publication Number | Publication Date |
|---|---|
| BRPI0705744A2 true BRPI0705744A2 (pt) | 2009-07-21 |
| BRPI0705744B1 BRPI0705744B1 (pt) | 2017-06-13 |
Family
ID=40667068
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0705744-0A BRPI0705744B1 (pt) | 2007-11-19 | 2007-11-19 | Composition of enzymes, use of composition in enzymatic hydrolysis of lignocellulose material, process of production of enzymes that degrades the fraction of polymacarides of the biomass, process of alcohol production using enzyme composition |
Country Status (2)
| Country | Link |
|---|---|
| BR (1) | BRPI0705744B1 (pt) |
| WO (1) | WO2009065199A1 (pt) |
Families Citing this family (5)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| CN113367160B (zh) * | 2021-06-09 | 2022-07-01 | 云南星耀生物制品有限公司 | 绿色木霉菌制剂在防治番茄灰霉病中的应用 |
| CN113667609B (zh) * | 2021-08-26 | 2023-05-12 | 泸州老窖股份有限公司 | 水解白酒丢糟的混合菌酶液的制备方法 |
| CN114058605B (zh) * | 2021-11-10 | 2023-05-12 | 湖北大学 | 一种提高纤维素酶比活力的方法及其应用 |
| CN115975819B (zh) * | 2022-11-21 | 2024-06-11 | 福建师范大学 | 一株糠醛耐受的里氏木霉突变菌株及其应用 |
| CN116083405B (zh) * | 2023-03-07 | 2023-09-05 | 中国科学院天津工业生物技术研究所 | 一种酒糟降解酶制剂及菌酶协同生产单细胞蛋白的方法 |
Family Cites Families (2)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| FI89613C (fi) * | 1991-12-05 | 1993-10-25 | Valtion Teknillinen | Foerfarande foer enzymatisk behandling av cellulosamassor |
| AU2003219956A1 (en) * | 2003-02-27 | 2004-09-28 | Midwest Research Institute | Superactive cellulase formulation using cellobiohydrolase-1 from penicillium funiculosum |
-
2007
- 2007-11-19 BR BRPI0705744-0A patent/BRPI0705744B1/pt active IP Right Grant
-
2008
- 2008-11-19 WO PCT/BR2008/000344 patent/WO2009065199A1/en not_active Ceased
Also Published As
| Publication number | Publication date |
|---|---|
| WO2009065199A1 (en) | 2009-05-28 |
| BRPI0705744B1 (pt) | 2017-06-13 |
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| B25A | Requested transfer of rights approved |
Owner name: FUNDACAO UNIVERSIDADE DE BRASILIA (BR/DF) , UNIVER Free format text: TRANSFERIDO DE: UNIVERSIDADE ESTADUAL DE CAMPINAS - UNICAMP |
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| B06F | Objections, documents and/or translations needed after an examination request according art. 34 industrial property law | ||
| B07A | Technical examination (opinion): publication of technical examination (opinion) | ||
| B06A | Notification to applicant to reply to the report for non-patentability or inadequacy of the application according art. 36 industrial patent law | ||
| B09A | Decision: intention to grant | ||
| B16A | Patent or certificate of addition of invention granted |