BRPI0800612A2 - método e kit para a quantificação de subclasses de igg humanas especìficas a alérgenos para o acompanhamento da imunoterapia especìfica - Google Patents
método e kit para a quantificação de subclasses de igg humanas especìficas a alérgenos para o acompanhamento da imunoterapia especìficaInfo
- Publication number
- BRPI0800612A2 BRPI0800612A2 BRPI0800612-1A BRPI0800612A BRPI0800612A2 BR PI0800612 A2 BRPI0800612 A2 BR PI0800612A2 BR PI0800612 A BRPI0800612 A BR PI0800612A BR PI0800612 A2 BRPI0800612 A2 BR PI0800612A2
- Authority
- BR
- Brazil
- Prior art keywords
- specific
- allergen
- kit
- antibodies
- der
- Prior art date
Links
- 238000000034 method Methods 0.000 title abstract 4
- 238000009169 immunotherapy Methods 0.000 title abstract 3
- 239000013566 allergen Substances 0.000 abstract 4
- 238000002965 ELISA Methods 0.000 abstract 2
- 102000004190 Enzymes Human genes 0.000 abstract 2
- 108090000790 Enzymes Proteins 0.000 abstract 2
- 208000028004 allergic respiratory disease Diseases 0.000 abstract 1
- 239000013642 negative control Substances 0.000 abstract 1
- 239000013641 positive control Substances 0.000 abstract 1
- 238000011002 quantification Methods 0.000 abstract 1
- 230000035945 sensitivity Effects 0.000 abstract 1
- 239000000758 substrate Substances 0.000 abstract 1
Classifications
-
- G—PHYSICS
- G01—MEASURING; TESTING
- G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
- G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
- G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
- G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
- G01N33/68—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing involving proteins, peptides or amino acids
- G01N33/6854—Immunoglobulins
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Engineering & Computer Science (AREA)
- Immunology (AREA)
- Molecular Biology (AREA)
- Urology & Nephrology (AREA)
- Biomedical Technology (AREA)
- Chemical & Material Sciences (AREA)
- Hematology (AREA)
- Food Science & Technology (AREA)
- Analytical Chemistry (AREA)
- Microbiology (AREA)
- Cell Biology (AREA)
- Biotechnology (AREA)
- Medicinal Chemistry (AREA)
- Physics & Mathematics (AREA)
- Proteomics, Peptides & Aminoacids (AREA)
- Biochemistry (AREA)
- General Health & Medical Sciences (AREA)
- General Physics & Mathematics (AREA)
- Pathology (AREA)
- Peptides Or Proteins (AREA)
- Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
Abstract
MéTODO E KIT PARA A QUANTIFICAçãO DE SUBCLASSES DE IgG HUMANAS ESPECìFICAS A ALéRGENOS PARA O ACOMPANHAMENTO DA IMUNOTERAPIA ESPECìFICA. A presente invenção tem como objetivo fornecer um método para quantificar níveis de subclasses de anticorpos IgG humanos, particularmente das subclasses IgGl, IgG2, IgG3 ou IgG4, específicos a alérgenos através de uma técnica imunoenzimática ELISA reversa, e um kit compreendendo placas de microtitulação para ELISA já sensibilizadas/revestidas com anticorpos monoclonais, por exemplo, anti-Der p 1 ou anti-Der p 2, extrato total de alérgenos correspondentes, anticorpos secundários marcados, conjugado enzimático, substrato enzimático e tampão cromógeno, além dos soros controles positivo e negativo de referência, proporcionando, assim, maior sensibilidade na detecção dos anticorpos específicos das subclasses de IgG, como uma potencial ferramenta para o acompanhamento da imunoterapia específica com alérgenos em pacientes com doença alérgica respiratória.
Priority Applications (4)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0800612-1A BRPI0800612A2 (pt) | 2008-01-02 | 2008-01-02 | método e kit para a quantificação de subclasses de igg humanas especìficas a alérgenos para o acompanhamento da imunoterapia especìfica |
| PCT/BR2008/000124 WO2009082798A1 (en) | 2008-01-02 | 2008-04-29 | Method and kit for quantifying of allergen-especific human igg subclasses for the control and attendance of specific immunotherapy |
| EP08733527.9A EP2232265B1 (en) | 2008-01-02 | 2008-04-29 | Method for quantifying allergen-especific human igg subclasses for the control and attendance of specific immunotherapy |
| US12/830,240 US20110008806A1 (en) | 2008-01-02 | 2010-07-02 | Method and kit for quantifying of allergen-specific human igg subclasses for the control and attendance of specific immunotherapy |
Applications Claiming Priority (1)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| BRPI0800612-1A BRPI0800612A2 (pt) | 2008-01-02 | 2008-01-02 | método e kit para a quantificação de subclasses de igg humanas especìficas a alérgenos para o acompanhamento da imunoterapia especìfica |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI0800612A2 true BRPI0800612A2 (pt) | 2009-08-25 |
Family
ID=40823716
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI0800612-1A BRPI0800612A2 (pt) | 2008-01-02 | 2008-01-02 | método e kit para a quantificação de subclasses de igg humanas especìficas a alérgenos para o acompanhamento da imunoterapia especìfica |
Country Status (4)
| Country | Link |
|---|---|
| US (1) | US20110008806A1 (pt) |
| EP (1) | EP2232265B1 (pt) |
| BR (1) | BRPI0800612A2 (pt) |
| WO (1) | WO2009082798A1 (pt) |
Families Citing this family (26)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| TWI507689B (zh) * | 2011-11-16 | 2015-11-11 | Taichung Veterans General Hospital Vacrs | Dust mite test specimen, detection kit and detection method |
| GB201511364D0 (en) * | 2015-06-29 | 2015-08-12 | Binding Site Group The Ltd | Method of detecting or monitoring an IgG4-related disease |
| CN106405090A (zh) * | 2015-08-14 | 2017-02-15 | 翁晖 | 一种用于鉴定每品种变应原制剂批间差异的方法 |
| KR102676276B1 (ko) * | 2017-01-18 | 2024-06-18 | 싸토리우스 바이오애널리티컬 인스트루먼츠, 아이엔씨 | 생물학적 샘플로부터 면역글로불린 감마 (IgG) 항체 아이소타입 농도를 결정하는 방법 |
| CN108614120A (zh) * | 2018-05-03 | 2018-10-02 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgG4抗体捕获气传过敏原的ELISA试剂盒 |
| CN108845122A (zh) * | 2018-05-03 | 2018-11-20 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgG3抗体检测气传过敏原的ELISA试剂盒 |
| CN108646024A (zh) * | 2018-05-03 | 2018-10-12 | 沈阳汇敏源生物科技有限责任公司 | 基于IgM抗体捕获食物过敏原的ELISA试剂盒 |
| CN108593931A (zh) * | 2018-05-03 | 2018-09-28 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgA抗体捕获气传过敏原的ELISA试剂盒 |
| CN108845124A (zh) * | 2018-05-03 | 2018-11-20 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgG1抗体检测气传过敏原的ELISA试剂盒 |
| CN108593932A (zh) * | 2018-05-03 | 2018-09-28 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgM抗体检测气传过敏原的ELISA试剂盒 |
| CN108646025A (zh) * | 2018-05-03 | 2018-10-12 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgG3抗体捕获气传过敏原的ELISA试剂盒 |
| CN108845137A (zh) * | 2018-05-03 | 2018-11-20 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG1抗体捕获食物过敏原的ELISA试剂盒 |
| CN108646026A (zh) * | 2018-05-03 | 2018-10-12 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG3抗体检测食物过敏原的ELISA试剂盒 |
| CN108845136A (zh) * | 2018-05-03 | 2018-11-20 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG3抗体捕获食物过敏原的ELISA试剂盒 |
| CN108845138A (zh) * | 2018-05-03 | 2018-11-20 | 沈阳汇敏源生物科技有限责任公司 | 基于IgM抗体检测食物过敏原的ELISA试剂盒 |
| CN108845123A (zh) * | 2018-05-03 | 2018-11-20 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgM抗体捕获气传过敏原的ELISA试剂盒 |
| CN108445234A (zh) * | 2018-05-03 | 2018-08-24 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG4抗体捕获食物过敏原的ELISA试剂盒 |
| CN108646023A (zh) * | 2018-05-03 | 2018-10-12 | 辽宁汇普源生物医学科技开发有限责任公司 | 基于IgG4抗体检测气传过敏原的ELISA试剂盒 |
| CN108593933A (zh) * | 2018-05-03 | 2018-09-28 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG1抗体检测食物过敏原的ELISA试剂盒 |
| CN108802359A (zh) * | 2018-07-06 | 2018-11-13 | 沈阳汇敏源生物科技有限责任公司 | 基于IgG2抗体检测食物过敏原的ELISA试剂盒 |
| CN108872566A (zh) * | 2018-07-19 | 2018-11-23 | 锦州医科大学 | 基于IgG2抗体检测气传过敏原的ELISA试剂盒 |
| CN109406770B (zh) * | 2018-09-30 | 2020-11-24 | 华南理工大学 | 一种双模式elisa酶免显色剂及其制备与应用 |
| CN111751416A (zh) * | 2020-07-03 | 2020-10-09 | 福建中医药大学 | 一种新型的美洲大蠊多肽快速检测方法 |
| CN112710837B (zh) * | 2020-11-03 | 2022-05-17 | 浙江大学 | 一种定量蒿属植物花粉中nsLTP过敏原的方法 |
| CN116298302A (zh) * | 2022-05-13 | 2023-06-23 | 杭州赛基生物科技有限公司 | 检测免疫球蛋白g亚类的试剂盒及其制备方法和使用方法 |
| CN116102654A (zh) * | 2022-07-08 | 2023-05-12 | 汕头大学医学院 | 一种双特异性抗体及其筛选方法与应用 |
Family Cites Families (9)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US4256833A (en) * | 1979-01-08 | 1981-03-17 | Majid Ali | Enzyme immunoassay for allergic disorders |
| JPS59208462A (ja) * | 1983-04-29 | 1984-11-26 | バイオウィッテッカー・インコーポレーテッド | キモパパインの分析法 |
| US5084379A (en) * | 1983-04-29 | 1992-01-28 | Biowhittaker, Inc. | Fluorometric assay of chymopapain hypersensitivity and reagents therefor |
| DE3586787T2 (de) * | 1984-05-11 | 1993-06-03 | Biowhittaker Inc | Fluoreszenztest fuer igg4. |
| FR2646917A1 (fr) * | 1989-05-12 | 1990-11-16 | Fond Nat Transfusion Sanguine | Procede de dosage d'anti-corps igg anti-allergenes specifiques, fraction riche en igg4 obtenue selon ce procede et composition pharmaceutique contenant cette fraction |
| JPH0862217A (ja) * | 1994-08-01 | 1996-03-08 | Nippon Zeon Co Ltd | 酵素免疫定量法用プレート |
| US6835824B1 (en) * | 1995-12-29 | 2004-12-28 | University Of Arkansas | Peanut allergens and methods |
| US20060008895A1 (en) * | 2004-07-08 | 2006-01-12 | Hsiu-Mei Chen | Microarray chip for detection of immunoglobulin |
| US20060160161A1 (en) * | 2004-10-26 | 2006-07-20 | Elan Pharmaceuticals, Inc. | Methods for assessing antibodies to neurodegenerative disease-associated antigens |
-
2008
- 2008-01-02 BR BRPI0800612-1A patent/BRPI0800612A2/pt not_active Application Discontinuation
- 2008-04-29 EP EP08733527.9A patent/EP2232265B1/en not_active Not-in-force
- 2008-04-29 WO PCT/BR2008/000124 patent/WO2009082798A1/en not_active Ceased
-
2010
- 2010-07-02 US US12/830,240 patent/US20110008806A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| US20110008806A1 (en) | 2011-01-13 |
| WO2009082798A1 (en) | 2009-07-09 |
| EP2232265B1 (en) | 2016-05-18 |
| EP2232265A4 (en) | 2010-12-29 |
| EP2232265A1 (en) | 2010-09-29 |
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Legal Events
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| B03A | Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette] | ||
| B06F | Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette] | ||
| B03H | Publication of an application: rectification [chapter 3.8 patent gazette] | ||
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Free format text: REPUBLICACAO REFERENTE AO DESPACHO 6.6 PUBLICADO NA RPI 2155 DE 24/04/2012, REGULARIZANDO O NOME DOS DEPOSITANTES FUNDACAO DE AMPARO A PESQUISA DO ESTADO DE MINAS GERAIS - FAPEMIG (BR/MG); ALERGOLAB - LABORATORIO DE INVESTIGACAO EM ALERGIA LTDA (BR/MG) E UNIVERSIDADE FEDERAL DE UBERLANDIA (BR/MG), ANTERIORMENTE PUBLICADO COM INCORRECAO NA RPI 2016 DE 25/08/2009 E RETIFICADO NA RPI 2189 DE 18/12/2012. |
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| B07D | Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette] | ||
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| B07A | Application suspended after technical examination (opinion) [chapter 7.1 patent gazette] | ||
| B09B | Patent application refused [chapter 9.2 patent gazette] | ||
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