BRPI0806252A2 - molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo - Google Patents

molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo Download PDF

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Publication number
BRPI0806252A2
BRPI0806252A2 BRPI0806252-8A BRPI0806252A BRPI0806252A2 BR PI0806252 A2 BRPI0806252 A2 BR PI0806252A2 BR PI0806252 A BRPI0806252 A BR PI0806252A BR PI0806252 A2 BRPI0806252 A2 BR PI0806252A2
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BR
Brazil
Prior art keywords
host cell
nucleic acid
acid molecule
recombinant vector
gene expression
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BRPI0806252-8A
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English (en)
Inventor
So Yeon Rah
Jae-Woo Jang
Sun-Young Lee
Young Hoon Park
Sang Jo Lim
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Cj Cheiljedang Corp
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Application filed by Cj Cheiljedang Corp filed Critical Cj Cheiljedang Corp
Publication of BRPI0806252A2 publication Critical patent/BRPI0806252A2/pt
Publication of BRPI0806252B1 publication Critical patent/BRPI0806252B1/pt
Publication of BRPI0806252B8 publication Critical patent/BRPI0806252B8/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/74—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora
    • C12N15/77—Vectors or expression systems specially adapted for prokaryotic hosts other than E. coli, e.g. Lactobacillus, Micromonospora for Corynebacterium; for Brevibacterium
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/66—General methods for inserting a gene into a vector to form a recombinant vector using cleavage and ligation; Use of non-functional linkers or adaptors, e.g. linkers containing the sequence for a restriction endonuclease

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  • Genetics & Genomics (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Chemical & Material Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Molecular Biology (AREA)
  • Microbiology (AREA)
  • Plant Pathology (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

MOLéCULA DE áCIDO NUCLéICO DE PROMOTOR, VETOR RECOMBINANTE, CéLULA DE HOSPEDEIRO E MéTODO DE EXPRESSãO DE GENE ALVO. São proporcionados uma molécula inovativa de ácido nucléico de promotor que tem uma sequência de nucleotideo de SEQ ID NO: 1 ou 2 derivada de Corynebacterium glutamicum, um vetor recombinante que compreende o promotor, uma célula de hospedeiro trans- formada com o vetor e um método de expressar genes de interesse usando a célula de hospedeiro.
BRPI0806252A 2007-01-15 2008-01-15 molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo BRPI0806252B8 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
KR1020070004386A KR100789274B1 (ko) 2007-01-15 2007-01-15 코리네박테리움 글루타미쿰에서 유래한 신규한 프로모터핵산 분자, 그 프로모터를 포함하는 재조합 벡터, 그재조합 벡터를 포함하는 숙주 세포 및 그 숙주 세포를이용하여 유전자를 발현하는 방법
PCT/KR2008/000246 WO2008088158A1 (en) 2007-01-15 2008-01-15 Novel promoter nucleic acid molecule derived from corynebacterium glutamicum, recombinant vector comprising the promoter, host cell comprising the recombinant vector and method of expressing gene using the host cell

Publications (3)

Publication Number Publication Date
BRPI0806252A2 true BRPI0806252A2 (pt) 2011-08-30
BRPI0806252B1 BRPI0806252B1 (pt) 2016-11-29
BRPI0806252B8 BRPI0806252B8 (pt) 2017-05-02

Family

ID=39216004

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0806252A BRPI0806252B8 (pt) 2007-01-15 2008-01-15 molécula de ácido nucléico de promotor, vetor recombinante, célula de hospedeiro e método de expressão de gene alvo

Country Status (7)

Country Link
US (1) US8426195B2 (pt)
JP (1) JP5266252B2 (pt)
KR (1) KR100789274B1 (pt)
CN (1) CN101605890B (pt)
BR (1) BRPI0806252B8 (pt)
DE (1) DE112008000181T9 (pt)
WO (1) WO2008088158A1 (pt)

Families Citing this family (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
KR101072708B1 (ko) * 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－크산틸산 생산능이 향상된 미생물 및 이를 이용한 ５＇－크산틸산의 생산방법
KR101072720B1 (ko) * 2008-12-17 2011-10-11 씨제이제일제당 (주) ５＇－구아닐산 생산능이 향상된 미생물 및 이를 이용한 ５＇－구아닐산의 생산방법
KR101226384B1 (ko) * 2010-03-05 2013-01-25 씨제이제일제당 (주) 개량된 프로모터 및 이를 이용한 ｌ-라이신의 생산 방법
DE102010003419B4 (de) 2010-03-30 2019-09-12 Evonik Degussa Gmbh Verfahren zur fermentativen Herstellung von L-Ornithin
CN103153044B (zh) 2010-10-08 2015-06-17 韩国生命工学研究院 源自巴西橡胶树的乳胶分泌组织特异性srpp启动子及其用途
WO2012067174A1 (ja) * 2010-11-18 2012-05-24 グリーンフェノール・高機能フェノール樹脂製造技術研究組合 コリネ型細菌形質転換体及びそれを用いるフェノールの製造方法
KR20140066553A (ko) 2012-11-23 2014-06-02 삼성전자주식회사 코리네박테리움 속 균주의 신규 프로모터
KR20140095850A (ko) 2013-01-25 2014-08-04 삼성전자주식회사 코리네박테리움과 대장균용 셔틀벡터
KR101677368B1 (ko) 2015-04-02 2016-11-18 씨제이제일제당 (주) 신규 프로모터 및 이의 용도
CN108350464A (zh) * 2015-12-07 2018-07-31 齐默尔根公司 来自谷氨酸棒杆菌的启动子
KR20180084756A (ko) 2015-12-07 2018-07-25 지머젠 인코포레이티드 코리네박테리움 글루타미컴으로부터의 프로모터
JP2020524492A (ja) * 2017-06-07 2020-08-20 ザイマージェン インコーポレイテッド Ｃｏｒｙｎｅｂａｃｔｅｒｉｕｍ ｇｌｕｔａｍｉｃｕｍ由来のプロモーターおよび補助遺伝子発現の制御におけるその使用
KR101925930B1 (ko) * 2017-07-24 2019-02-26 씨제이제일제당 주식회사 Ｌ-라이신을 생산하는 미생물 및 이를 이용한 ｌ-라이신의 생산 방법
KR102016050B1 (ko) * 2018-03-20 2019-08-29 씨제이제일제당 주식회사 신규한 프로모터 및 이의 용도
WO2022152036A1 (zh) * 2021-01-12 2022-07-21 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其在生产目标化合物中的用途
CN115322989B (zh) * 2021-05-10 2024-09-27 中国科学院天津工业生物技术研究所 具有启动子活性的多核苷酸及其用途和生产目标化合物的方法
CN112877352B (zh) * 2021-02-08 2023-03-21 华南理工大学 一种适用于谷氨酸棒状杆菌的cumate诱导系统及该诱导系统构建的质粒载体和应用

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JP4623825B2 (ja) * 1999-12-16 2011-02-02 協和発酵バイオ株式会社 新規ポリヌクレオチド
JP2003265177A (ja) * 2002-03-13 2003-09-24 Gekkeikan Sake Co Ltd タンパク質の高発現システム
CN1842601A (zh) * 2003-08-27 2006-10-04 奥夫莱夫塔埃克尼公司 通过靶向抑制内源贮藏蛋白增强植物种子中异源多肽的累积
DE102004035065A1 (de) * 2004-07-20 2006-02-16 Basf Ag P-ET-TS-Expressionseinheiten
KR100620092B1 (ko) 2004-12-16 2006-09-08 씨제이 주식회사 코리네박테리움 속 세포로부터 유래된 신규한 프로모터서열, 그를 포함하는 발현 카세트 및 벡터, 상기 벡터를포함하는 숙주 세포 및 그를 이용하여 유전자를 발현하는방법
JP2006214933A (ja) 2005-02-04 2006-08-17 Seiko Epson Corp 測位システム、端末装置、端末装置の制御方法、端末装置の制御プログラム、端末装置の制御プログラムを記録したコンピュータ読み取り可能な記録媒体
JP4700395B2 (ja) 2005-04-13 2011-06-15 株式会社豊田中央研究所 酸性条件下で使用するためのプロモーター及びその利用
JP5756259B2 (ja) 2006-09-15 2015-07-29 シージェイ チェイルジェダン コーポレイション Ｌ−リジン生産能の向上したコリネバクテリウム属およびそれを用いたｌ−リジン生産方法

Also Published As

Publication number Publication date
DE112008000181T5 (de) 2009-12-10
CN101605890A (zh) 2009-12-16
CN101605890B (zh) 2013-05-01
US20100261256A1 (en) 2010-10-14
KR100789274B1 (ko) 2008-01-02
US8426195B2 (en) 2013-04-23
BRPI0806252B1 (pt) 2016-11-29
WO2008088158A1 (en) 2008-07-24
BRPI0806252B8 (pt) 2017-05-02
DE112008000181T9 (de) 2010-04-08
JP5266252B2 (ja) 2013-08-21
JP2010515453A (ja) 2010-05-13

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B06A Notification to applicant to reply to the report for non-patentability or inadequacy of the application [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 15/01/2008, OBSERVADAS AS CONDICOES LEGAIS.

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