BRPI0806831B8 - métodos atuadores de gotículas empregando esferas magnéticas - Google Patents

métodos atuadores de gotículas empregando esferas magnéticas

Info

Publication number
BRPI0806831B8
BRPI0806831B8 BRPI0806831A BRPI0806831A BRPI0806831B8 BR PI0806831 B8 BRPI0806831 B8 BR PI0806831B8 BR PI0806831 A BRPI0806831 A BR PI0806831A BR PI0806831 A BRPI0806831 A BR PI0806831A BR PI0806831 B8 BRPI0806831 B8 BR PI0806831B8
Authority
BR
Brazil
Prior art keywords
droplet
reactive
magnetic force
spheres
initial
Prior art date
Application number
BRPI0806831A
Other languages
English (en)
Inventor
E Eckhardt Allen
G Pollack Michael
Sista Ramakrishna
K Pamula Vamsee
Srinivasan Vijay
Original Assignee
Advanced Liquid Logic Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Advanced Liquid Logic Inc filed Critical Advanced Liquid Logic Inc
Publication of BRPI0806831A2 publication Critical patent/BRPI0806831A2/pt
Publication of BRPI0806831B1 publication Critical patent/BRPI0806831B1/pt
Publication of BRPI0806831B8 publication Critical patent/BRPI0806831B8/pt

Links

Classifications

    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54313—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being characterised by its particulate form
    • G01N33/54326—Magnetic particles
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502715—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by interfacing components, e.g. fluidic, electrical, optical or mechanical interfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/50273—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by the means or forces applied to move the fluids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502761—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip specially adapted for handling suspended solids or molecules independently from the bulk fluid flow, e.g. for trapping or sorting beads or physically stretching molecules
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/01—Drops
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12M—APPARATUS FOR ENZYMOLOGY OR MICROBIOLOGY; APPARATUS FOR CULTURING MICROORGANISMS FOR PRODUCING BIOMASS, FOR GROWING CELLS OR FOR OBTAINING FERMENTATION OR METABOLIC PRODUCTS, i.e. BIOREACTORS OR FERMENTERS
    • C12M25/00—Means for supporting, enclosing or fixing the microorganisms, e.g. immunocoatings
    • C12M25/16—Particles; Beads; Granular material; Encapsulation
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44704—Details; Accessories
    • G01N27/44717—Arrangements for investigating the separated zones, e.g. localising zones
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N27/00—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means
    • G01N27/26—Investigating or analysing materials by the use of electric, electrochemical, or magnetic means by investigating electrochemical variables; by using electrolysis or electrophoresis
    • G01N27/416—Systems
    • G01N27/447—Systems using electrophoresis
    • G01N27/44756—Apparatus specially adapted therefor
    • G01N27/44786—Apparatus specially adapted therefor of the magneto-electrophoresis type
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/54366—Apparatus specially adapted for solid-phase testing
    • G01N33/54386—Analytical elements
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/50—Chemical analysis of biological material, e.g. blood, urine; Testing involving biospecific ligand binding methods; Immunological testing
    • G01N33/53—Immunoassay; Biospecific binding assay; Materials therefor
    • G01N33/543—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals
    • G01N33/551—Immunoassay; Biospecific binding assay; Materials therefor with an insoluble carrier for immobilising immunochemicals the carrier being inorganic
    • G01N33/553—Metal or metal coated
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/02—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor using a plurality of sample containers moved by a conveyor system past one or more treatment or analysis stations
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/025—Align devices or objects to ensure defined positions relative to each other
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/02—Adapting objects or devices to another
    • B01L2200/028—Modular arrangements
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0647—Handling flowable solids, e.g. microscopic beads, cells, particles
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0673—Handling of plugs of fluid surrounded by immiscible fluid
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/14—Process control and prevention of errors
    • B01L2200/141—Preventing contamination, tampering
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0864—Configuration of multiple channels and/or chambers in a single devices comprising only one inlet and multiple receiving wells, e.g. for separation, splitting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0861—Configuration of multiple channels and/or chambers in a single devices
    • B01L2300/0867—Multiple inlets and one sample wells, e.g. mixing, dilution
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0427—Electrowetting
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/043—Moving fluids with specific forces or mechanical means specific forces magnetic forces
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/10—Devices for transferring samples or any liquids to, in, or from, the analysis apparatus, e.g. suction devices, injection devices
    • G01N2035/1027—General features of the devices
    • G01N2035/1034—Transferring microquantities of liquid
    • G01N2035/1046—Levitated, suspended drops
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N35/00—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor
    • G01N35/0098—Automatic analysis not limited to methods or materials provided for in any single one of groups G01N1/00 - G01N33/00; Handling materials therefor involving analyte bound to insoluble magnetic carrier, e.g. using magnetic separation

Landscapes

  • Health & Medical Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Immunology (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • Analytical Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Biochemistry (AREA)
  • Physics & Mathematics (AREA)
  • Urology & Nephrology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Dispersion Chemistry (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Pathology (AREA)
  • Organic Chemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Clinical Laboratory Science (AREA)
  • Food Science & Technology (AREA)
  • Cell Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Sustainable Development (AREA)
  • Electrochemistry (AREA)
  • Fluid Mechanics (AREA)
  • Inorganic Chemistry (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Physical Or Chemical Processes And Apparatus (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Soft Magnetic Materials (AREA)
  • Micromachines (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

dispositivos atuadores de gotículas e métodos empregando esferas magnéticas trata-se de um método para proporcionar uma gotícula em contato com uma esfera reativa à força magnética e contendo uma quantidade reduzida de uma substância. o método em geral inclui as etapas de a(a) proporcionar um atuador de. gotículas compreendendo: (i) um substrato compreendendo eletrodos configurados para realizar operações de gotícula em uma superfície; (ii) uma gotícula inicial, compreendendo: (1) uma ou mais esferas reativas à força magnética; (2) uma quantidade inicial da substância; e (3) um volume inicial; (b) imobilizar magneticamente a uma ou mais esferas reativas à força magnética em uma localização que se encontra a uma distância de uma zona de divisão da gotícula alvo; (c) realizar uma ou mais operações de gotícula compreendendo operações de divisão de gotícula selecionadas para dividir a gotícula combinada para produzir um conjunto de gotícula, compreendendo: (i) uma gotícula compreendendo substancialmente todas da uma ou mais esferas reativas à força magnética e contendo uma quantidade reduzida da substância em relação à concentração inicial; e (ii) uma gotícula substancialmente sem as esferas reativas à força magnética.
BRPI0806831A 2007-02-09 2008-02-11 métodos atuadores de gotículas empregando esferas magnéticas BRPI0806831B8 (pt)

Applications Claiming Priority (9)

Application Number Priority Date Filing Date Title
US90065307P 2007-02-09 2007-02-09
US60/900,653 2007-02-09
US96973607P 2007-09-04 2007-09-04
US60/969,736 2007-09-04
US98076207P 2007-10-17 2007-10-17
US98077207P 2007-10-17 2007-10-17
US60/980,762 2007-10-17
US60/980,772 2007-10-17
PCT/US2008/053545 WO2008098236A2 (en) 2007-02-09 2008-02-11 Droplet actuator devices and methods employing magnetic beads

Publications (3)

Publication Number Publication Date
BRPI0806831A2 BRPI0806831A2 (pt) 2014-05-13
BRPI0806831B1 BRPI0806831B1 (pt) 2019-06-25
BRPI0806831B8 true BRPI0806831B8 (pt) 2021-07-27

Family

ID=39495992

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0806831A BRPI0806831B8 (pt) 2007-02-09 2008-02-11 métodos atuadores de gotículas empregando esferas magnéticas

Country Status (11)

Country Link
US (4) US9046514B2 (pt)
EP (4) EP2570812B1 (pt)
JP (2) JP5156762B2 (pt)
KR (2) KR101431778B1 (pt)
CN (1) CN101627308B (pt)
AU (1) AU2008212808B2 (pt)
BR (1) BRPI0806831B8 (pt)
CA (2) CA2712863C (pt)
DK (1) DK2111554T3 (pt)
ES (1) ES2423930T3 (pt)
WO (1) WO2008098236A2 (pt)

Families Citing this family (134)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
AU2006207933B2 (en) 2005-01-28 2010-11-18 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8716015B2 (en) 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
US8470606B2 (en) 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
WO2010042637A2 (en) * 2008-10-07 2010-04-15 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2010027894A2 (en) * 2008-08-27 2010-03-11 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
WO2008091848A2 (en) 2007-01-22 2008-07-31 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
CA2712863C (en) 2007-02-09 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
EP2109774B1 (en) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US8440392B2 (en) 2007-03-22 2013-05-14 Advanced Liquid Logic Inc. Method of conducting a droplet based enzymatic assay
US8202686B2 (en) 2007-03-22 2012-06-19 Advanced Liquid Logic, Inc. Enzyme assays for a droplet actuator
CN101743304B (zh) * 2007-04-10 2013-04-24 先进流体逻辑公司 微滴分配设备和方法
US8951732B2 (en) 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
AU2008293652B2 (en) 2007-08-24 2013-02-21 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
US20100236928A1 (en) * 2007-10-17 2010-09-23 Advanced Liquid Logic, Inc. Multiplexed Detection Schemes for a Droplet Actuator
WO2009052095A1 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Reagent storage and reconstitution for a droplet actuator
EP2212683A4 (en) * 2007-10-17 2011-08-31 Advanced Liquid Logic Inc MANIPULATION OF BEADS INTO DROPLETS
WO2009052354A2 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Droplet actuator structures
US20100236929A1 (en) * 2007-10-18 2010-09-23 Advanced Liquid Logic, Inc. Droplet Actuators, Systems and Methods
EP2232535A4 (en) 2007-12-10 2016-04-13 Advanced Liquid Logic Inc DROPLET ACTUATOR CONFIGURATIONS AND METHODS
MX2010007034A (es) * 2007-12-23 2010-09-14 Advanced Liquid Logic Inc Configuraciones para eyector de gotas y metodos para realizar operaciones de gota.
US8617899B2 (en) * 2008-02-14 2013-12-31 Palo Alto Research Center Incorporated Enhanced drop mixing using magnetic actuation
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
DK2279405T3 (da) * 2008-05-13 2014-01-13 Advanced Liquid Logic Inc Dråbeaktuatorindretninger, -systemer og -fremgangsmåder
US20110097763A1 (en) * 2008-05-13 2011-04-28 Advanced Liquid Logic, Inc. Thermal Cycling Method
EP2286228B1 (en) * 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
WO2010077859A2 (en) * 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
WO2010120080A2 (ko) * 2009-04-14 2010-10-21 서울대학교산학협력단 컬러 코드화된 자성 구조물
EP2599548B1 (de) * 2009-05-13 2018-07-04 ibidi GmbH Probenträger zum Positionieren einer organischen, biologischen und/oder medizinischen Probe
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
EP2539450B1 (en) * 2010-02-25 2016-02-17 Advanced Liquid Logic, Inc. Method of making nucleic acid libraries
EP2553473A4 (en) 2010-03-30 2016-08-10 Advanced Liquid Logic Inc DROPLET OPERATION PLATFORM
US10232374B2 (en) 2010-05-05 2019-03-19 Miroculus Inc. Method of processing dried samples using digital microfluidic device
WO2012012090A2 (en) 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012009320A2 (en) * 2010-07-15 2012-01-19 Advanced Liquid Logic, Inc. Systems for and methods of promoting cell lysis in droplet actuators
EP2641097A4 (en) 2010-11-17 2016-09-07 CAPACITY RECOGNITION IN A DROPPER ACTUATOR
ES2837407T3 (es) 2011-04-05 2021-06-30 Purdue Research Foundation Sistema microfluídico usando microaberturas para detección con alto rendimiento de entidades
WO2012154745A2 (en) 2011-05-09 2012-11-15 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
WO2012154794A2 (en) 2011-05-10 2012-11-15 Advanced Liquid Logic, Inc. Enzyme concentration and assays
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
KR20140064771A (ko) * 2011-07-06 2014-05-28 어드밴스드 리퀴드 로직, 아이엔씨. 비말 작동기 상의 시약 저장
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
KR20130009504A (ko) 2011-07-15 2013-01-23 삼성전자주식회사 개구 조절 방법 및 개구 조절 소자
WO2013016413A2 (en) 2011-07-25 2013-01-31 Advanced Liquid Logic Inc Droplet actuator apparatus and system
US8637242B2 (en) 2011-11-07 2014-01-28 Illumina, Inc. Integrated sequencing apparatuses and methods of use
US9709469B2 (en) * 2011-11-11 2017-07-18 The Regents Of The University Of California Valveless microfluidic device
WO2013078216A1 (en) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Glucose-6-phosphate dehydrogenase assays
US9068695B2 (en) * 2012-06-12 2015-06-30 Smrt Delivery Llc Active guidance of fluid agents using magnetorheological antibubbles
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
CN104540594B (zh) * 2012-06-25 2019-07-02 通用医疗公司 使用高梯度磁场对粒子进行分类
CN104603595B (zh) 2012-06-27 2017-08-08 先进流体逻辑公司 用于减少气泡形成的技术和液滴致动器设计
US10316872B2 (en) * 2012-08-27 2019-06-11 The Board Of Trustees Of The Leland Stanford Junior University Two-dimensional magnetic trap arrays for droplet control
CA2885546C (en) * 2012-10-05 2018-06-05 Cornell University Enzymes forming mesoporous assemblies embedded in macroporous scaffolds
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
US20140322706A1 (en) 2012-10-24 2014-10-30 Jon Faiz Kayyem Integrated multipelx target analysis
JP1628115S (pt) 2012-10-24 2019-04-01
US9366647B2 (en) * 2013-03-14 2016-06-14 Taiwan Semiconductor Manufacturing Company, Ltd. Optical detection for bio-entities
US9752968B2 (en) 2012-12-21 2017-09-05 Luminex Corporation Rotating shielded magnetic actuator
US9636689B2 (en) 2012-12-21 2017-05-02 Luminex Corporation Rotating magnetic actuator
US9492824B2 (en) * 2013-01-16 2016-11-15 Sharp Kabushiki Kaisha Efficient dilution method, including washing method for immunoassay
CN107015013B (zh) 2013-01-31 2018-12-21 卢米耐克斯公司 准备测定的系统和方法
EP3520895A1 (en) 2013-03-15 2019-08-07 Genmark Diagnostics Inc. Fluid container with cantilevered lance
US9341639B2 (en) 2013-07-26 2016-05-17 Industrial Technology Research Institute Apparatus for microfluid detection
WO2015023747A1 (en) 2013-08-13 2015-02-19 Advanced Liquid Logic, Inc. Methods of improving accuracy and precision of droplet metering using an on-actuator reservoir as the fluid input
CA2920390A1 (en) 2013-08-30 2015-03-05 Illumina, Inc. Manipulation of droplets on hydrophilic or variegated-hydrophilic surfaces
US9498778B2 (en) 2014-11-11 2016-11-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
USD881409S1 (en) 2013-10-24 2020-04-14 Genmark Diagnostics, Inc. Biochip cartridge
CN105658784B (zh) 2013-12-18 2018-12-11 东洋制罐集团控股株式会社 培养容器、淋巴细胞的培养方法、培养容器的制造方法和固相化装置
TWI510780B (zh) * 2014-03-20 2015-12-01 Univ Nat Chiao Tung 生物檢測設備及生物晶片
CN106459967A (zh) 2014-04-29 2017-02-22 Illumina公司 使用模板转换和标签化的多重单细胞基因表达分析
JP2016067277A (ja) * 2014-09-30 2016-05-09 セイコーエプソン株式会社 生体関連物質抽出デバイス及び生体関連物質抽出装置
CN106999850B (zh) 2014-10-09 2020-04-07 亿明达股份有限公司 用于分离不混溶的液体以有效地隔离至少一种液体的方法和装置
US10005080B2 (en) 2014-11-11 2018-06-26 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system employing electrowetting fluid manipulation
US9598722B2 (en) 2014-11-11 2017-03-21 Genmark Diagnostics, Inc. Cartridge for performing assays in a closed sample preparation and reaction system
WO2016077364A2 (en) 2014-11-11 2016-05-19 Genmark Diagnostics, Inc. Instrument and cartridge for performing assays in a closed sample preparation and reaction system
WO2016087397A1 (en) * 2014-12-02 2016-06-09 Koninklijke Philips N.V. Dispersion and accumulation of magnetic particles in a microfluidic system
EP3059588B1 (en) * 2015-02-18 2018-01-10 Fundacion Tekniker Method and device for detection and quantification of analytes
EP3061529A1 (en) * 2015-02-24 2016-08-31 AdnaGen GmbH Apparatus and method for the analysis, isolation and/or enrichment of target structures in a fluid sample
US10576471B2 (en) 2015-03-20 2020-03-03 Illumina, Inc. Fluidics cartridge for use in the vertical or substantially vertical position
EP3303548A4 (en) 2015-06-05 2019-01-02 Miroculus Inc. Evaporation management in digital microfluidic devices
WO2016197103A1 (en) 2015-06-05 2016-12-08 Miroculus Inc. Air-matrix digital microfluidics apparatuses and methods for limiting evaporation and surface fouling
RU2709986C2 (ru) 2015-08-14 2019-12-23 Иллюмина, Инк. Системы и способы применения магниточувствительных датчиков для определения генетической характеристики
CN115928221A (zh) 2015-08-28 2023-04-07 Illumina公司 单细胞核酸序列分析
HK1253910A1 (zh) 2015-09-02 2019-07-05 伊卢米纳剑桥有限公司 改善流控系统中的液滴操作的系统和方法
US10450598B2 (en) 2015-09-11 2019-10-22 Illumina, Inc. Systems and methods for obtaining a droplet having a designated concentration of a substance-of-interest
CA3002983A1 (en) 2015-10-22 2017-04-27 Illumina, Inc. Filler fluid for fluidic devices
EP3383538B1 (en) 2015-12-01 2022-03-09 Illumina, Inc. Liquid storage and delivery mechanisms
WO2017095917A1 (en) 2015-12-01 2017-06-08 Illumina, Inc. Digital microfluidic system for single-cell isolation and characterization of analytes
CA3016221C (en) 2016-04-07 2021-09-28 Illumina, Inc. Methods and systems for construction of normalized nucleic acid libraries
EP3488256B1 (en) 2016-07-21 2025-06-18 Siemens Healthcare Diagnostics Inc. Magnetic shielding for ivd automation system
CN109715781A (zh) 2016-08-22 2019-05-03 米罗库鲁斯公司 用于数字微流控设备中的并行液滴控制的反馈系统
WO2018053501A1 (en) 2016-09-19 2018-03-22 Genmark Diagnostics, Inc. Instrument for processing cartridge for performing assays in a closed sample preparation and reaction system
JP2020515815A (ja) 2016-12-28 2020-05-28 ミロキュラス インコーポレイテッド デジタルマイクロ流体デバイスおよび方法
CA3057175A1 (en) * 2017-03-31 2018-10-04 The Governing Council Of The University Of Toronto Methods for the filtration of small-volume heterogeneous suspensions in a digital microfluidic device
WO2018187476A1 (en) 2017-04-04 2018-10-11 Miroculus Inc. Digital microfluidic apparatuses and methods for manipulating and processing encapsulated droplets
CN110892258A (zh) 2017-07-24 2020-03-17 米罗库鲁斯公司 具有集成的血浆收集设备的数字微流控系统和方法
WO2019046860A1 (en) 2017-09-01 2019-03-07 Miroculus Inc. DIGITAL MICROFLUIDIC DEVICES AND METHODS OF USE
DE102018125403A1 (de) * 2017-10-13 2019-04-18 Maxim Integrated Products, Inc. Analytensensor-package mit abgabechemie und mikrofluidkappe
IL312691A (en) 2018-01-29 2024-07-01 St Jude Childrens Res Hospital Inc Method for nucleic acid amplification
US11666914B2 (en) 2018-05-09 2023-06-06 Tecan Trading Ag Cartridge, electrowetting sample processing system and bead manipulation method
EP3796999A4 (en) 2018-05-23 2022-03-09 Miroculus Inc. Control of evaporation in digital microfluidics
JP7396777B2 (ja) 2018-09-28 2023-12-12 オクタン バイオテック インコーポレーテッド 磁気分離
EP3917670A4 (en) 2019-01-31 2022-11-02 Miroculus Inc. ANTIFOULING COMPOSITIONS AND METHODS FOR HANDLING AND TREATING ENCAPSULATED DROPLET
CN109603942B (zh) * 2019-02-15 2021-12-24 京东方科技集团股份有限公司 微流控装置和微流控方法
CN113766975B (zh) 2019-02-28 2023-12-29 米罗库鲁斯公司 数字微流控设备及其使用方法
CN119158636A (zh) 2019-04-08 2024-12-20 米罗库鲁斯公司 多盒式数字微流控装置和使用方法
US11618021B2 (en) 2019-06-28 2023-04-04 Wisconsin Alumni Research Foundation Volume-free reagent addition and exclusion-based sample preparation for streamlined multi-step assays
CA3143224A1 (en) 2019-07-10 2021-01-14 National Research Council Of Canada Oil residue protection in oil-encapsulated digital microfluidics
US11524298B2 (en) 2019-07-25 2022-12-13 Miroculus Inc. Digital microfluidics devices and methods of use thereof
MX2022001324A (es) 2019-07-31 2022-05-19 Bioskryb Genomics Inc Análisis de células individuales.
CN114450090B (zh) * 2019-09-10 2024-07-23 深圳华大智造控股有限公司 磁珠子在微流控基板上的操作
KR102308065B1 (ko) 2019-11-08 2021-09-30 경북대학교 산학협력단 입자검출장치 및 이를 이용한 입자검출방법
US11970692B2 (en) * 2019-12-13 2024-04-30 Becton, Dickinson And Company Magnet assembly to prevent extraction particle carryover
CN117121115A (zh) 2020-06-12 2023-11-24 豪夫迈·罗氏有限公司 用于评定诊断设备上的舱室的系统和方法
US20230304066A1 (en) 2020-09-04 2023-09-28 Baebies, Inc. Microfluidic based assay for unbound bilirubin
US11951476B2 (en) * 2020-11-19 2024-04-09 Rutgers, The State University Of New Jersey Electronic-sensing and magnetic-modulation (ESMM) biosensor for phagocytosis quantification in pathogenic infections and methods of use thereof
CN112934280B (zh) * 2021-02-01 2023-03-10 上海中航光电子有限公司 一种微流控装置及其检测方法
CN113578406A (zh) * 2021-09-07 2021-11-02 北京航空航天大学 液滴转移用微柱表面、微柱表面制备方法及液滴转移方法
US11772093B2 (en) 2022-01-12 2023-10-03 Miroculus Inc. Methods of mechanical microfluidic manipulation
DE102022200663A1 (de) 2022-01-21 2023-07-27 Robert Bosch Gesellschaft mit beschränkter Haftung Mikrofluidische Vorrichtung und Verfahren zu ihrem Betrieb
KR20250137709A (ko) * 2023-01-30 2025-09-18 아이덱스 래보러토리즈, 인코포레이티드 전기습윤 바코드 자성 비드를 위한 방법, 시스템 및 디바이스
CN118513306A (zh) * 2024-05-23 2024-08-20 广东奥素液芯微纳科技有限公司 基于数字微流控的磁珠洗涤方法及其应用

Family Cites Families (245)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US647486A (en) 1899-10-05 1900-04-17 Wellis R Fox Acetylene-gas generator.
US4127460A (en) 1976-10-27 1978-11-28 Desoto, Inc. Radiation-curing aqueous coatings providing a nonadherent surface
US4244693A (en) 1977-02-28 1981-01-13 The United States Of America As Represented By The United States Department Of Energy Method and composition for testing for the presence of an alkali metal
FR2543320B1 (fr) * 1983-03-23 1986-01-31 Thomson Csf Dispositif indicateur a commande electrique de deplacement d'un fluide
US5038852A (en) 1986-02-25 1991-08-13 Cetus Corporation Apparatus and method for performing automated amplification of nucleic acid sequences and assays using heating and cooling steps
KR970007077B1 (ko) * 1987-03-13 1997-05-02 코울터 일렉트로닉스 인커퍼레이티드 광산란 기술을 이용한 다중-부분식별 분석 방법
US6013531A (en) 1987-10-26 2000-01-11 Dade International Inc. Method to use fluorescent magnetic polymer particles as markers in an immunoassay
US5225332A (en) 1988-04-22 1993-07-06 Massachusetts Institute Of Technology Process for manipulation of non-aqueous surrounded microdroplets
GB8917963D0 (en) 1989-08-05 1989-09-20 Scras Apparatus for repeated automatic execution of a thermal cycle for treatment of biological samples
US5266498A (en) 1989-10-27 1993-11-30 Abbott Laboratories Ligand binding assay for an analyte using surface-enhanced scattering (SERS) signal
SE467309B (sv) * 1990-10-22 1992-06-29 Berol Nobel Ab Hydrofiliserad fast yta, foerfarande foer dess framstaellning samt medel daerfoer
US5181016A (en) * 1991-01-15 1993-01-19 The United States Of America As Represented By The United States Department Of Energy Micro-valve pump light valve display
IL100866A (en) * 1991-02-06 1995-10-31 Igen Inc Method and apparatus for magnetic microparticulate based luminescence assay including plurality of magnets
US5370842A (en) 1991-11-29 1994-12-06 Canon Kabushiki Kaisha Sample measuring device and sample measuring system
US5498392A (en) 1992-05-01 1996-03-12 Trustees Of The University Of Pennsylvania Mesoscale polynucleotide amplification device and method
WO1994008759A1 (en) 1992-10-16 1994-04-28 Thomas Jefferson University Method and apparatus for robotically performing sanger dideoxynucleotide dna sequencing reactions
US5472881A (en) 1992-11-12 1995-12-05 University Of Utah Research Foundation Thiol labeling of DNA for attachment to gold surfaces
US6152181A (en) 1992-11-16 2000-11-28 The United States Of America As Represented By The Secretary Of The Air Force Microdevices based on surface tension and wettability that function as sensors, actuators, and other devices
EP0636413B1 (en) 1993-07-28 2001-11-14 PE Corporation (NY) Nucleic acid amplification reaction apparatus and method
US5486337A (en) * 1994-02-18 1996-01-23 General Atomics Device for electrostatic manipulation of droplets
US6613560B1 (en) * 1994-10-19 2003-09-02 Agilent Technologies, Inc. PCR microreactor for amplifying DNA using microquantities of sample fluid
US6673533B1 (en) 1995-03-10 2004-01-06 Meso Scale Technologies, Llc. Multi-array multi-specific electrochemiluminescence testing
US6319668B1 (en) 1995-04-25 2001-11-20 Discovery Partners International Method for tagging and screening molecules
CA2176053C (en) 1995-05-09 1999-10-05 Yoshihiro Kinoshita Method and apparatus for agglutination immunoassay
US6130098A (en) 1995-09-15 2000-10-10 The Regents Of The University Of Michigan Moving microdroplets
US5945281A (en) 1996-02-02 1999-08-31 Becton, Dickinson And Company Method and apparatus for determining an analyte from a sample fluid
IT1291608B1 (it) 1997-04-18 1999-01-11 Sisme Immobiliare S P A Disposizione a motore sincrono monofase a magneti permanenti
DE19717085C2 (de) 1997-04-23 1999-06-17 Bruker Daltonik Gmbh Verfahren und Geräte für extrem schnelle DNA-Vervielfachung durch Polymerase-Kettenreaktionen (PCR)
US5998224A (en) 1997-05-16 1999-12-07 Abbott Laboratories Magnetically assisted binding assays utilizing a magnetically responsive reagent
US20020001544A1 (en) 1997-08-28 2002-01-03 Robert Hess System and method for high throughput processing of droplets
US7214298B2 (en) * 1997-09-23 2007-05-08 California Institute Of Technology Microfabricated cell sorter
WO1999026067A1 (en) 1997-11-18 1999-05-27 Bio-Rad Laboratories, Inc. Multiplex flow immunoassays with magnetic particles as solid phase
DE19822123C2 (de) 1997-11-21 2003-02-06 Meinhard Knoll Verfahren und Vorrichtung zum Nachweis von Analyten
US6063339A (en) 1998-01-09 2000-05-16 Cartesian Technologies, Inc. Method and apparatus for high-speed dot array dispensing
GB9820755D0 (en) 1998-09-23 1998-11-18 Xaar Technology Ltd Drop on demand ink jet printing apparatus
US6591852B1 (en) * 1998-10-13 2003-07-15 Biomicro Systems, Inc. Fluid circuit components based upon passive fluid dynamics
US6565727B1 (en) 1999-01-25 2003-05-20 Nanolytics, Inc. Actuators for microfluidics without moving parts
US6294063B1 (en) * 1999-02-12 2001-09-25 Board Of Regents, The University Of Texas System Method and apparatus for programmable fluidic processing
AU3498900A (en) 1999-02-23 2000-09-14 Caliper Technologies Corporation Sequencing by incorporation
US6225061B1 (en) * 1999-03-10 2001-05-01 Sequenom, Inc. Systems and methods for performing reactions in an unsealed environment
DE60036746T2 (de) 1999-03-25 2008-07-24 Tosoh Corp., Shinnanyo Analysator
IT1309430B1 (it) 1999-05-18 2002-01-23 Guerrieri Roberto Metodo ed apparato per la manipolazione di particelle per mezzo delladielettroforesi
US20020051971A1 (en) 1999-05-21 2002-05-02 John R. Stuelpnagel Use of microfluidic systems in the detection of target analytes using microsphere arrays
FR2794039B1 (fr) * 1999-05-27 2002-05-03 Osmooze Sa Dispositif de formation, de deplacement et de diffusion de petites quantites calibrees de liquides
US6977145B2 (en) 1999-07-28 2005-12-20 Serono Genetics Institute S.A. Method for carrying out a biochemical protocol in continuous flow in a microreactor
DE19938002A1 (de) * 1999-08-11 2001-02-15 Studiengesellschaft Kohle Mbh Beschichtung mit quervernetzten hydrophilen Polymeren
US20030027204A1 (en) 1999-09-03 2003-02-06 Yokogawa Electric Corporation, A Japan Corporation Method and apparatus for producing biochips
US20040209376A1 (en) 1999-10-01 2004-10-21 Surromed, Inc. Assemblies of differentiable segmented particles
ATE328670T1 (de) 1999-11-11 2006-06-15 Trinity College Dublin Vorrichtung und verfahren zur verabreichung von tropfen
US6432290B1 (en) * 1999-11-26 2002-08-13 The Governors Of The University Of Alberta Apparatus and method for trapping bead based reagents within microfluidic analysis systems
JP4240729B2 (ja) * 2000-02-14 2009-03-18 日油株式会社 臨床検査用微粒子分散剤、検査用試薬、試薬の製造方法、検査方法および用途
US6924792B1 (en) * 2000-03-10 2005-08-02 Richard V. Jessop Electrowetting and electrostatic screen display systems, colour displays and transmission means
JP3442338B2 (ja) 2000-03-17 2003-09-02 株式会社日立製作所 Ｄｎａ分析装置、ｄｎａ塩基配列決定装置、ｄｎａ塩基配列決定方法、および反応モジュール
WO2002007503A1 (en) 2000-07-25 2002-01-31 The Regents Of The University Of California Electrowetting-driven micropumping
CA2314398A1 (en) 2000-08-10 2002-02-10 Edward Shipwash Microarrays and microsystems for amino acid analysis and protein sequencing
US6773566B2 (en) 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
AU2001290879A1 (en) * 2000-09-15 2002-03-26 California Institute Of Technology Microfabricated crossflow devices and methods
US6453928B1 (en) 2001-01-08 2002-09-24 Nanolab Ltd. Apparatus, and method for propelling fluids
US7010391B2 (en) 2001-03-28 2006-03-07 Handylab, Inc. Methods and systems for control of microfluidic devices
US7211442B2 (en) 2001-06-20 2007-05-01 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
US7179423B2 (en) 2001-06-20 2007-02-20 Cytonome, Inc. Microfluidic system including a virtual wall fluid interface port for interfacing fluids with the microfluidic system
JP4004018B2 (ja) * 2001-06-29 2007-11-07 株式会社東芝 周波数変換器及びこれを用いた無線通信装置
WO2003076892A2 (en) * 2001-08-06 2003-09-18 Vanderbilt University Device and methods for measuring the response of at least one cell to a medium
US6734436B2 (en) 2001-08-07 2004-05-11 Sri International Optical microfluidic devices and methods
US6995024B2 (en) 2001-08-27 2006-02-07 Sri International Method and apparatus for electrostatic dispensing of microdroplets
AU2002359508A1 (en) * 2001-11-26 2003-06-10 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
US20040231987A1 (en) 2001-11-26 2004-11-25 Keck Graduate Institute Method, apparatus and article for microfluidic control via electrowetting, for chemical, biochemical and biological assays and the like
GB0129068D0 (en) * 2001-12-05 2002-01-23 Koninl Philips Electronics Nv Display device
US6733172B2 (en) 2002-03-11 2004-05-11 The Regents Of The University Of California Magnetohydrodynamic (MHD) driven droplet mixer
US7147763B2 (en) 2002-04-01 2006-12-12 Palo Alto Research Center Incorporated Apparatus and method for using electrostatic force to cause fluid movement
US6958132B2 (en) 2002-05-31 2005-10-25 The Regents Of The University Of California Systems and methods for optical actuation of microfluidics based on opto-electrowetting
FR2841063B1 (fr) * 2002-06-18 2004-09-17 Commissariat Energie Atomique Dispositif de deplacement de petits volumes de liquide le long d'un micro-catenaire par des forces electrostatiques
JP2006507921A (ja) 2002-06-28 2006-03-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ 流体分散のための方法および装置
FR2842747B1 (fr) 2002-07-23 2004-10-15 Commissariat Energie Atomique Procede et dispositif pour le criblage de molecules dans des cellules
FR2843048B1 (fr) * 2002-08-01 2004-09-24 Commissariat Energie Atomique Dispositif d'injection et de melange de micro-gouttes liquides.
JP4115207B2 (ja) * 2002-08-30 2008-07-09 キヤノン株式会社 インクジェット記録装置
US6911132B2 (en) 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
US7329545B2 (en) * 2002-09-24 2008-02-12 Duke University Methods for sampling a liquid flow
US6989234B2 (en) * 2002-09-24 2006-01-24 Duke University Method and apparatus for non-contact electrostatic actuation of droplets
US20040055871A1 (en) 2002-09-25 2004-03-25 The Regents Of The University Of California Use of ion beams for protecting substrates from particulate defect contamination in ultra-low-defect coating processes
US7217542B2 (en) 2002-10-31 2007-05-15 Hewlett-Packard Development Company, L.P. Microfluidic system for analyzing nucleic acids
US7547380B2 (en) * 2003-01-13 2009-06-16 North Carolina State University Droplet transportation devices and methods having a fluid surface
GB0304033D0 (en) 2003-02-21 2003-03-26 Imp College Innovations Ltd Apparatus
US7041481B2 (en) 2003-03-14 2006-05-09 The Regents Of The University Of California Chemical amplification based on fluid partitioning
JP4921163B2 (ja) * 2003-05-06 2012-04-25 コーニンクレッカ フィリップス エレクトロニクス エヌ ヴィ 切替え可能素子における駆動電圧の低減
JP4404672B2 (ja) 2003-05-28 2010-01-27 セイコーエプソン株式会社 液滴吐出ヘッド、液滴吐出ヘッドの製造方法、マイクロアレイ製造装置、及びマイクロアレイの製造方法
JP4432374B2 (ja) 2003-06-17 2010-03-17 セイコーエプソン株式会社 ヘッドキャッピング機構及びこれを備えた液滴吐出装置
JP2005037346A (ja) 2003-06-25 2005-02-10 Aisin Seiki Co Ltd マイクロ流体制御システム
TWI230760B (en) * 2003-07-29 2005-04-11 Univ Tsinghua Electrowetting electrode design with electromagnetic field for actuation of the magnetic-beads biochemical detection system
TW594007B (en) * 2003-08-15 2004-06-21 Prec Instr Dev Ct Nat Biochemical detection device and method of magnetic fluid bead control combining digital fluid and electromagnetic field
US7767435B2 (en) 2003-08-25 2010-08-03 University Of Washington Method and device for biochemical detection and analysis of subcellular compartments from a single cell
CA2479452C (en) * 2003-08-30 2008-11-04 F.Hoffmann-La Roche Ag Method and device for determining analytes in a liquid
AU2004283721B2 (en) * 2003-10-24 2009-08-13 Adhesives Research, Inc. Rapidly disintegrating film
JP2005139011A (ja) 2003-11-04 2005-06-02 Nof Corp 火薬原料及びその製造方法
ATE434131T1 (de) * 2003-11-17 2009-07-15 Koninkl Philips Electronics Nv System zur handhabung einer fluidmenge
WO2005068971A1 (en) * 2004-01-14 2005-07-28 Luminex Corporation Methods and systems for dynamic range expansion
KR20070037432A (ko) * 2004-01-15 2007-04-04 도꾸리쯔교세이호징 가가꾸 기쥬쯔 신꼬 기꼬 화학 분석장치 및 화학 분석방법
FR2866493B1 (fr) * 2004-02-16 2010-08-20 Commissariat Energie Atomique Dispositif de controle du deplacement d'une goutte entre deux ou plusieurs substrats solides
US7495031B2 (en) 2004-02-24 2009-02-24 Kao Corporation Process for producing an emulsion
KR100552706B1 (ko) 2004-03-12 2006-02-20 삼성전자주식회사 핵산 증폭 방법 및 장치
US7048889B2 (en) 2004-03-23 2006-05-23 Lucent Technologies Inc. Dynamically controllable biological/chemical detectors having nanostructured surfaces
WO2005090997A1 (ja) * 2004-03-23 2005-09-29 Toray Industries, Inc. 溶液を攪拌する方法
US20050226991A1 (en) 2004-04-07 2005-10-13 Hossainy Syed F Methods for modifying balloon of a catheter assembly
KR100583231B1 (ko) 2004-04-13 2006-05-26 한국과학기술연구원 물방울형 세포 부유액을 이용한 세포 분리 장치
JP2007536634A (ja) 2004-05-04 2007-12-13 フィッシャー−ローズマウント・システムズ・インコーポレーテッド プロセス制御システムのためのサービス指向型アーキテクチャ
EP3144066A1 (en) 2004-05-28 2017-03-22 Board of Regents, The University of Texas System Programmable fluidic processors
FR2871076A1 (fr) 2004-06-04 2005-12-09 Univ Lille Sciences Tech Dispositif pour desorption par rayonnement laser incorporant une manipulation de l'echantillon liquide sous forme de gouttes individuelles permettant leur traitement chimique et biochimique
US7121998B1 (en) 2004-06-08 2006-10-17 Eurica Califorrniaa Vented microcradle for prenidial incubator
FR2872438B1 (fr) 2004-07-01 2006-09-15 Commissariat Energie Atomique Dispositif de deplacement et de traitement de volumes de liquide
US7693666B2 (en) * 2004-07-07 2010-04-06 Rensselaer Polytechnic Institute Method, system, and program product for controlling chemical reactions in a digital microfluidic system
FR2872715B1 (fr) * 2004-07-08 2006-11-17 Commissariat Energie Atomique Microreacteur goutte
FR2872809B1 (fr) * 2004-07-09 2006-09-15 Commissariat Energie Atomique Methode d'adressage d'electrodes
US7267752B2 (en) 2004-07-28 2007-09-11 University Of Rochester Rapid flow fractionation of particles combining liquid and particulate dielectrophoresis
JP2006058031A (ja) 2004-08-17 2006-03-02 Hitachi High-Technologies Corp 化学分析装置
EP1789195B1 (en) 2004-08-26 2010-10-27 Life Technologies Corporation Electrowetting dispensing devices and related methods
JP4047314B2 (ja) 2004-09-07 2008-02-13 株式会社東芝 微細流路構造体
JP5885901B2 (ja) 2004-09-09 2016-03-16 アンスティテュート キュリー マイクロチャネルまたは他のマイクロ容器中でパケットを操作するためのデバイス
JP4185904B2 (ja) 2004-10-27 2008-11-26 株式会社日立ハイテクノロジーズ 液体搬送基板、分析システム、分析方法
WO2007044029A2 (en) * 2004-12-03 2007-04-19 Nano Science Diagnostic, Inc. Method and apparatus for low quantity detection of bioparticles in small sample volumes
FR2879946B1 (fr) 2004-12-23 2007-02-09 Commissariat Energie Atomique Dispositif de dispense de gouttes
US7458661B2 (en) * 2005-01-25 2008-12-02 The Regents Of The University Of California Method and apparatus for promoting the complete transfer of liquid drops from a nozzle
AU2006207933B2 (en) 2005-01-28 2010-11-18 Duke University Apparatuses and methods for manipulating droplets on a printed circuit board
US7454988B2 (en) 2005-02-10 2008-11-25 Applera Corporation Method for fluid sampling using electrically controlled droplets
US20060210443A1 (en) 2005-03-14 2006-09-21 Stearns Richard G Avoidance of bouncing and splashing in droplet-based fluid transport
JP4559274B2 (ja) 2005-03-30 2010-10-06 シャープ株式会社 画像表示装置
FR2884437B1 (fr) * 2005-04-19 2007-07-20 Commissariat Energie Atomique Dispositif et procede microfluidique de transfert de matiere entre deux phases immiscibles.
JP2008539759A (ja) 2005-05-11 2008-11-20 ナノリティックス・インコーポレイテッド 多数の温度で生化学的又は化学的な反応を実施する方法及び装置
JP4547301B2 (ja) 2005-05-13 2010-09-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス及び分析システム
JP2006317364A (ja) 2005-05-16 2006-11-24 Hitachi High-Technologies Corp 分注装置
CN101237934B (zh) 2005-05-21 2012-12-19 先进液体逻辑公司 用亲水性聚合物助剂减弱生物分子的吸附
JP2006329904A (ja) 2005-05-30 2006-12-07 Hitachi High-Technologies Corp 液体搬送デバイス及び分析システム
JP4500733B2 (ja) 2005-05-30 2010-07-14 株式会社日立ハイテクノロジーズ 化学分析装置
JP4969060B2 (ja) 2005-06-08 2012-07-04 株式会社日立ハイテクノロジーズ 自動分析装置
EP1890815A1 (en) * 2005-06-16 2008-02-27 Core-Microsolutions, Inc. Biosensor detection by means of droplet driving, agitation, and evaporation
FR2887305B1 (fr) 2005-06-17 2011-05-27 Commissariat Energie Atomique Dispositif de pompage par electromouillage et application aux mesures d'activite electrique
EP1899048B1 (fr) * 2005-07-01 2008-12-17 Commissariat A L'energie Atomique Revetement de surface hydrophobe et a faible hysteresis de mouillage, procede de depot d'un tel revetement, micro-composant et utilisation
FR2888912B1 (fr) * 2005-07-25 2007-08-24 Commissariat Energie Atomique Procede de commande d'une communication entre deux zones par electromouillage, dispositif comportant des zones isolables les unes des autres et procede de realisation d'un tel dispositif
US20070023292A1 (en) * 2005-07-26 2007-02-01 The Regents Of The University Of California Small object moving on printed circuit board
US7556776B2 (en) 2005-09-08 2009-07-07 President And Fellows Of Harvard College Microfluidic manipulation of fluids and reactions
EP4071458B1 (en) * 2005-09-21 2025-11-05 Luminex Corporation Methods and systems for image data processing
FR2890875B1 (fr) * 2005-09-22 2008-02-22 Commissariat Energie Atomique Fabrication d'un systeme diphasique liquide/liquide ou gaz en micro-fluidique
US20070075922A1 (en) 2005-09-28 2007-04-05 Jessop Richard V Electronic display systems
US7344679B2 (en) * 2005-10-14 2008-03-18 International Business Machines Corporation Method and apparatus for point of care osmolarity testing
WO2007048111A2 (en) 2005-10-22 2007-04-26 Core-Microsolutions, Inc. Droplet extraction from a liquid column for on-chip microfluidics
EP3431994B1 (en) 2005-12-21 2024-03-06 Meso Scale Technologies, LLC. Assay modules having assay reagents and methods of making and using same
EP2364774A3 (en) 2006-01-11 2014-06-04 Raindance Technologies, Inc. Microfluidic Devices And Methods Of Use In The Formation And Control Of Nanoreactors
US20070207513A1 (en) * 2006-03-03 2007-09-06 Luminex Corporation Methods, Products, and Kits for Identifying an Analyte in a Sample
US8637317B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Method of washing beads
WO2010006166A2 (en) 2008-07-09 2010-01-14 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8492168B2 (en) 2006-04-18 2013-07-23 Advanced Liquid Logic Inc. Droplet-based affinity assays
US8613889B2 (en) 2006-04-13 2013-12-24 Advanced Liquid Logic, Inc. Droplet-based washing
WO2010027894A2 (en) 2008-08-27 2010-03-11 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7815871B2 (en) 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet microactuator system
US7816121B2 (en) * 2006-04-18 2010-10-19 Advanced Liquid Logic, Inc. Droplet actuation system and method
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US7901947B2 (en) * 2006-04-18 2011-03-08 Advanced Liquid Logic, Inc. Droplet-based particle sorting
JP5054096B2 (ja) * 2006-04-18 2012-10-24 アドヴァンスト リキッド ロジック インコーポレイテッド 液滴に基づく生化学
US8685754B2 (en) 2006-04-18 2014-04-01 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for immunoassays and washing
US8716015B2 (en) * 2006-04-18 2014-05-06 Advanced Liquid Logic, Inc. Manipulation of cells on a droplet actuator
WO2007123908A2 (en) * 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Droplet-based multiwell operations
US7727723B2 (en) 2006-04-18 2010-06-01 Advanced Liquid Logic, Inc. Droplet-based pyrosequencing
US8658111B2 (en) * 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US7763471B2 (en) * 2006-04-18 2010-07-27 Advanced Liquid Logic, Inc. Method of electrowetting droplet operations for protein crystallization
US8637324B2 (en) * 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
US8470606B2 (en) 2006-04-18 2013-06-25 Duke University Manipulation of beads in droplets and methods for splitting droplets
WO2010042637A2 (en) 2008-10-07 2010-04-15 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8980198B2 (en) 2006-04-18 2015-03-17 Advanced Liquid Logic, Inc. Filler fluids for droplet operations
US9675972B2 (en) 2006-05-09 2017-06-13 Advanced Liquid Logic, Inc. Method of concentrating beads in a droplet
US7822510B2 (en) * 2006-05-09 2010-10-26 Advanced Liquid Logic, Inc. Systems, methods, and products for graphically illustrating and controlling a droplet actuator
US8041463B2 (en) * 2006-05-09 2011-10-18 Advanced Liquid Logic, Inc. Modular droplet actuator drive
EP2021103B1 (en) 2006-05-09 2017-07-12 Advanced Liquid Logic, Inc. Electrowetting droplet microactuator controlled via graphical user interface
WO2009026339A2 (en) 2007-08-20 2009-02-26 Advanced Liquid Logic, Inc. Modular droplet actuator drive
US7939021B2 (en) 2007-05-09 2011-05-10 Advanced Liquid Logic, Inc. Droplet actuator analyzer with cartridge
ATE540750T1 (de) 2006-05-11 2012-01-15 Raindance Technologies Inc Mikrofluidische vorrichtung und verfahren
US8179216B2 (en) 2006-06-06 2012-05-15 University Of Virginia Patent Foundation Capillary force actuator device and related method of applications
US7629124B2 (en) 2006-06-30 2009-12-08 Canon U.S. Life Sciences, Inc. Real-time PCR in micro-channels
JP4844263B2 (ja) * 2006-07-07 2011-12-28 株式会社島津製作所 微量化学反応方法及び装置
JP4881950B2 (ja) 2006-07-10 2012-02-22 株式会社日立ハイテクノロジーズ 液体搬送デバイス
EP1905513A1 (en) 2006-09-13 2008-04-02 Institut Curie Methods and devices for sampling fluids
JP4901410B2 (ja) 2006-10-10 2012-03-21 シャープ株式会社 バックライト装置及び映像表示装置
US9266076B2 (en) * 2006-11-02 2016-02-23 The Regents Of The University Of California Method and apparatus for real-time feedback control of electrical manipulation of droplets on chip
FR2909293B1 (fr) 2006-12-05 2011-04-22 Commissariat Energie Atomique Micro-dispositif de traitement d'echantillons liquides
CA2672034C (en) 2006-12-13 2014-02-18 Luminex Corporation Systems and methods for multiplex analysis of pcr in real time
US8338166B2 (en) 2007-01-04 2012-12-25 Lawrence Livermore National Security, Llc Sorting, amplification, detection, and identification of nucleic acid subsequences in a complex mixture
WO2008091848A2 (en) 2007-01-22 2008-07-31 Advanced Liquid Logic, Inc. Surface assisted fluid loading and droplet dispensing
CA2712863C (en) 2007-02-09 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods employing magnetic beads
EP2109774B1 (en) 2007-02-15 2018-07-04 Advanced Liquid Logic, Inc. Capacitance detection in a droplet actuator
US20100025250A1 (en) 2007-03-01 2010-02-04 Advanced Liquid Logic, Inc. Droplet Actuator Structures
WO2008109664A1 (en) 2007-03-05 2008-09-12 Advanced Liquid Logic, Inc. Hydrogen peroxide droplet-based assays
CA2717154A1 (en) 2007-03-13 2008-09-18 Advanced Liquid Logic, Inc. Droplet actuator devices, configurations, and methods for improving absorbance detection
WO2011084703A2 (en) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Enzyme assays on a droplet actuator
US8093062B2 (en) 2007-03-22 2012-01-10 Theodore Winger Enzymatic assays using umbelliferone substrates with cyclodextrins in droplets in oil
US8440392B2 (en) 2007-03-22 2013-05-14 Advanced Liquid Logic Inc. Method of conducting a droplet based enzymatic assay
WO2008116221A1 (en) 2007-03-22 2008-09-25 Advanced Liquid Logic, Inc. Bead sorting on a droplet actuator
US8202686B2 (en) 2007-03-22 2012-06-19 Advanced Liquid Logic, Inc. Enzyme assays for a droplet actuator
EP2136920A2 (en) 2007-03-23 2009-12-30 Advanced Liquid Logic, Inc. Droplet actuator loading and target concentration
CN101743304B (zh) 2007-04-10 2013-04-24 先进流体逻辑公司 微滴分配设备和方法
US20100130369A1 (en) 2007-04-23 2010-05-27 Advanced Liquid Logic, Inc. Bead-Based Multiplexed Analytical Methods and Instrumentation
WO2009011952A1 (en) 2007-04-23 2009-01-22 Advanced Liquid Logic, Inc. Device and method for sample collection and concentration
US20100087012A1 (en) 2007-04-23 2010-04-08 Advanced Liquid Logic, Inc. Sample Collector and Processor
US20080283414A1 (en) 2007-05-17 2008-11-20 Monroe Charles W Electrowetting devices
US8951732B2 (en) 2007-06-22 2015-02-10 Advanced Liquid Logic, Inc. Droplet-based nucleic acid amplification in a temperature gradient
WO2009003184A1 (en) 2007-06-27 2008-12-31 Digital Biosystems Digital microfluidics based apparatus for heat-exchanging chemical processes
US20100120130A1 (en) 2007-08-08 2010-05-13 Advanced Liquid Logic, Inc. Droplet Actuator with Droplet Retention Structures
US20110303542A1 (en) 2007-08-08 2011-12-15 Advanced Liquid Logic, Inc. Use of Additives for Enhancing Droplet Operations
WO2009021233A2 (en) 2007-08-09 2009-02-12 Advanced Liquid Logic, Inc. Pcb droplet actuator fabrication
AU2008293652B2 (en) 2007-08-24 2013-02-21 Advanced Liquid Logic, Inc. Bead manipulations on a droplet actuator
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
EP2212683A4 (en) 2007-10-17 2011-08-31 Advanced Liquid Logic Inc MANIPULATION OF BEADS INTO DROPLETS
US20100236928A1 (en) 2007-10-17 2010-09-23 Advanced Liquid Logic, Inc. Multiplexed Detection Schemes for a Droplet Actuator
WO2009052354A2 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Droplet actuator structures
WO2009052095A1 (en) 2007-10-17 2009-04-23 Advanced Liquid Logic, Inc. Reagent storage and reconstitution for a droplet actuator
US20100236929A1 (en) 2007-10-18 2010-09-23 Advanced Liquid Logic, Inc. Droplet Actuators, Systems and Methods
US7621059B2 (en) 2007-10-18 2009-11-24 Oceaneering International, Inc. Underwater sediment evacuation system
EP2232535A4 (en) 2007-12-10 2016-04-13 Advanced Liquid Logic Inc DROPLET ACTUATOR CONFIGURATIONS AND METHODS
MX2010007034A (es) 2007-12-23 2010-09-14 Advanced Liquid Logic Inc Configuraciones para eyector de gotas y metodos para realizar operaciones de gota.
WO2009135205A2 (en) 2008-05-02 2009-11-05 Advanced Liquid Logic, Inc. Droplet actuator techniques using coagulatable samples
US8852952B2 (en) 2008-05-03 2014-10-07 Advanced Liquid Logic, Inc. Method of loading a droplet actuator
US20110097763A1 (en) 2008-05-13 2011-04-28 Advanced Liquid Logic, Inc. Thermal Cycling Method
DK2279405T3 (da) 2008-05-13 2014-01-13 Advanced Liquid Logic Inc Dråbeaktuatorindretninger, -systemer og -fremgangsmåder
US8093064B2 (en) 2008-05-15 2012-01-10 The Regents Of The University Of California Method for using magnetic particles in droplet microfluidics
EP2286228B1 (en) 2008-05-16 2019-04-03 Advanced Liquid Logic, Inc. Droplet actuator devices and methods for manipulating beads
FR2933713B1 (fr) 2008-07-11 2011-03-25 Commissariat Energie Atomique Procede et dispositif de manipulation et d'observation de gouttes de liquide
US20120261264A1 (en) 2008-07-18 2012-10-18 Advanced Liquid Logic, Inc. Droplet Operations Device
EP2331251A4 (en) 2008-08-13 2017-03-08 Advanced Liquid Logic, Inc. Methods, systems, and products for conducting droplet operations
WO2010077859A2 (en) 2008-12-15 2010-07-08 Advanced Liquid Logic, Inc. Nucleic acid amplification and sequencing on a droplet actuator
US8877512B2 (en) 2009-01-23 2014-11-04 Advanced Liquid Logic, Inc. Bubble formation techniques using physical or chemical features to retain a gas bubble within a droplet actuator
WO2011002957A2 (en) 2009-07-01 2011-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
WO2011020011A2 (en) 2009-08-13 2011-02-17 Advanced Liquid Logic, Inc. Droplet actuator and droplet-based techniques
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US8846414B2 (en) 2009-09-29 2014-09-30 Advanced Liquid Logic, Inc. Detection of cardiac markers on a droplet actuator
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
EP2553473A4 (en) 2010-03-30 2016-08-10 Advanced Liquid Logic Inc DROPLET OPERATION PLATFORM
WO2012012090A2 (en) 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Droplet actuator assemblies and methods of making same
WO2012009320A2 (en) 2010-07-15 2012-01-19 Advanced Liquid Logic, Inc. Systems for and methods of promoting cell lysis in droplet actuators
US20130168250A1 (en) 2010-09-16 2013-07-04 Advanced Liquid Logic Inc Droplet Actuator Systems, Devices and Methods
US8829171B2 (en) 2011-02-10 2014-09-09 Illumina, Inc. Linking sequence reads using paired code tags
CA2821299C (en) 2010-11-05 2019-02-12 Frank J. Steemers Linking sequence reads using paired code tags
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
EP2641097A4 (en) 2010-11-17 2016-09-07 CAPACITY RECOGNITION IN A DROPPER ACTUATOR
EP3037536B1 (en) 2011-01-28 2019-11-27 Illumina, Inc. Oligonucleotide replacement for di-tagged and directional libraries
US20130018611A1 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic Inc Systems and Methods of Measuring Gap Height
US9513253B2 (en) 2011-07-11 2016-12-06 Advanced Liquid Logic, Inc. Droplet actuators and techniques for droplet-based enzymatic assays
US20130017544A1 (en) 2011-07-11 2013-01-17 Advanced Liquid Logic Inc High Resolution Melting Analysis on a Droplet Actuator

Also Published As

Publication number Publication date
CA2712863C (en) 2015-01-06
CN101627308B (zh) 2013-08-14
US20250155430A1 (en) 2025-05-15
WO2008098236A3 (en) 2009-01-15
US9046514B2 (en) 2015-06-02
CA2856143A1 (en) 2008-08-14
EP2570811A3 (en) 2013-12-11
US20150314293A1 (en) 2015-11-05
CN101627308A (zh) 2010-01-13
KR101431778B1 (ko) 2014-08-20
ES2423930T3 (es) 2013-09-25
EP2570811A2 (en) 2013-03-20
EP2111554A2 (en) 2009-10-28
US20100068764A1 (en) 2010-03-18
DK2111554T3 (da) 2013-07-22
AU2008212808B2 (en) 2013-09-12
AU2008212808A1 (en) 2008-08-14
US20190324026A1 (en) 2019-10-24
JP2013029524A (ja) 2013-02-07
EP2570812A2 (en) 2013-03-20
BRPI0806831B1 (pt) 2019-06-25
CA2712863A1 (en) 2008-08-14
JP2010518403A (ja) 2010-05-27
CA2856143C (en) 2016-11-01
JP5156762B2 (ja) 2013-03-06
EP2573562A2 (en) 2013-03-27
JP5833521B2 (ja) 2015-12-16
EP2570812B1 (en) 2018-07-18
EP2570812A3 (en) 2013-12-11
EP2573562A3 (en) 2013-10-30
BRPI0806831A2 (pt) 2014-05-13
US10379112B2 (en) 2019-08-13
US12181467B2 (en) 2024-12-31
KR20100014917A (ko) 2010-02-11
KR20140078763A (ko) 2014-06-25
EP2570811B1 (en) 2014-11-26
EP2111554B1 (en) 2013-05-08
WO2008098236A2 (en) 2008-08-14
KR101503510B1 (ko) 2015-03-18

Similar Documents

Publication Publication Date Title
BRPI0806831B8 (pt) métodos atuadores de gotículas empregando esferas magnéticas
BRPI0616071B8 (pt) métodos de revestir uma micropartícula cristalina pré-formada em suspensão com um agente ativo
BR112014017899A8 (pt) Filtro químico dessecante a base de favo de mel e método de fabricação do mesmo
BR112012030585A2 (pt) sistema de controle para deslocamento em um comboio
BR112013016876A2 (pt) métodos e composições para um sistema de espressão transiente de soja in-planta
Peek et al. The shikimate dehydrogenase family: functional diversity within a conserved structural and mechanistic framework
WO2009158024A3 (en) Microfluidic droplets for metabolic engineering and other applications
BR112015018053A2 (pt) tinta de jato de tinta, método de gravação por jato de tinta e material gravado por tinta
UY25351A1 (es) Neutralizacion de policationes en un dispositivo cromatografico para uso con sangre completa.
WO2011084703A3 (en) Enzyme assays on a droplet actuator
AR060015A1 (es) Sistema de liberacion de plaguicida
AR074321A1 (es) Remocion de materiales objetivo utilizando metales de tierras raras
BRPI0511551A (pt) método, software e equipamento para utilizar informações de histórico de estado de aplicativo quando relançar aplicativos
CO6160256A2 (es) Sistema y metodo para generar estadisticas de uso de las aplicaciones que identifican la ubicacion
BR112014024811A8 (pt) Meio para receber um jato de tinta, método de impressão,composição de revestimento de pré-tratamento de um substrato.
BR112012031894A2 (pt) composição aquosa curável por radiação, revestimento, adesivo, uma tinta ou um verniz de sobreimpressão, uso de uma composição, artigo, e, método de revestimento de um artigo com uma composição
BR112015019534A8 (pt) processo para seleção de substrato sólido com base no derivado do esqueleto de organismo marinho otimizado para promoção de crescimento celular ou de tecido ou função restaurada, processo para conversão de substrato sólido subótimo em substrato sólido otimizado para promoção de crescimento celular ou de tecido ou função restaurada, substrato sólido e kit compreendendo o referido substrato sólido
BR112019000965A2 (pt) material de mancal de deslizamento de alumínio livre de chumbo com superfície funcional
BR112018074091B8 (pt) Métodos para desinfecção
BR112012026757A2 (pt) membro de adsorção e aparelho que utiliza o mesmo
McKelvey et al. Chemical form of selenium differentially influences DNA repair pathways following exposure to lead nitrate
BR112014004327A2 (pt) aparelho de apoio de direção e método de apoio de direção
BR112018002097A2 (pt) métodos de distribuição de micróbios ou enzimas produtoras de carbonato de cálcio furo abaixo
BR112015014699A2 (pt) película de silicone
BR112014017162A8 (pt) modulação de biologia vegetal

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 25/06/2019, OBSERVADAS AS CONDICOES LEGAIS. (CO) 10 (DEZ) ANOS CONTADOS A PARTIR DE 25/06/2019, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 11/02/2008, OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF, QUE DETERMINA A ALTERACAO DO PRAZO DE CONCESSAO