FR2933713B1 - Procede et dispositif de manipulation et d'observation de gouttes de liquide - Google Patents

Procede et dispositif de manipulation et d'observation de gouttes de liquide

Info

Publication number
FR2933713B1
FR2933713B1 FR0854745A FR0854745A FR2933713B1 FR 2933713 B1 FR2933713 B1 FR 2933713B1 FR 0854745 A FR0854745 A FR 0854745A FR 0854745 A FR0854745 A FR 0854745A FR 2933713 B1 FR2933713 B1 FR 2933713B1
Authority
FR
France
Prior art keywords
handling
liquid drops
observing liquid
observing
drops
Prior art date
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.)
Active
Application number
FR0854745A
Other languages
English (en)
Other versions
FR2933713A1 (fr
Inventor
Olivier Fuchs
Starace Fabien Sauter
Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)
Commissariat a lEnergie Atomique et aux Energies Alternatives CEA
Original Assignee
Commissariat a lEnergie Atomique CEA
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Commissariat a lEnergie Atomique CEA filed Critical Commissariat a lEnergie Atomique CEA
Priority to FR0854745A priority Critical patent/FR2933713B1/fr
Priority to EP09793960A priority patent/EP2318136A1/fr
Priority to US13/003,105 priority patent/US20110147215A1/en
Priority to PCT/EP2009/058777 priority patent/WO2010004014A1/fr
Publication of FR2933713A1 publication Critical patent/FR2933713A1/fr
Application granted granted Critical
Publication of FR2933713B1 publication Critical patent/FR2933713B1/fr
Active legal-status Critical Current
Anticipated expiration legal-status Critical

Links

Classifications

    • F—MECHANICAL ENGINEERING; LIGHTING; HEATING; WEAPONS; BLASTING
    • F04—POSITIVE - DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS FOR LIQUIDS OR ELASTIC FLUIDS
    • F04B—POSITIVE-DISPLACEMENT MACHINES FOR LIQUIDS; PUMPS
    • F04B19/00—Machines or pumps having pertinent characteristics not provided for in, or of interest apart from, groups F04B1/00 - F04B17/00
    • F04B19/006—Micropumps
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L3/00—Containers or dishes for laboratory use, e.g. laboratory glassware; Droppers
    • B01L3/50—Containers for the purpose of retaining a material to be analysed, e.g. test tubes
    • B01L3/502—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures
    • B01L3/5027—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip
    • B01L3/502769—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements
    • B01L3/502784—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics
    • B01L3/502792—Containers for the purpose of retaining a material to be analysed, e.g. test tubes with fluid transport, e.g. in multi-compartment structures by integrated microfluidic structures, i.e. dimensions of channels and chambers are such that surface tension forces are important, e.g. lab-on-a-chip characterised by multiphase flow arrangements specially adapted for droplet or plug flow, e.g. digital microfluidics for moving individual droplets on a plate, e.g. by locally altering surface tension
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2200/00—Solutions for specific problems relating to chemical or physical laboratory apparatus
    • B01L2200/06—Fluid handling related problems
    • B01L2200/0678—Facilitating or initiating evaporation
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/06—Auxiliary integrated devices, integrated components
    • B01L2300/0627—Sensor or part of a sensor is integrated
    • B01L2300/0645—Electrodes
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/0809—Geometry, shape and general structure rectangular shaped
    • B01L2300/0816—Cards, e.g. flat sample carriers usually with flow in two horizontal directions
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/08—Geometry, shape and general structure
    • B01L2300/089—Virtual walls for guiding liquids
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2300/00—Additional constructional details
    • B01L2300/16—Surface properties and coatings
    • B01L2300/161—Control and use of surface tension forces, e.g. hydrophobic, hydrophilic
    • B01L2300/165—Specific details about hydrophobic, oleophobic surfaces
    • B—PERFORMING OPERATIONS; TRANSPORTING
    • B01—PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL
    • B01L—CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE
    • B01L2400/00—Moving or stopping fluids
    • B01L2400/04—Moving fluids with specific forces or mechanical means
    • B01L2400/0403—Moving fluids with specific forces or mechanical means specific forces
    • B01L2400/0415—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic
    • B01L2400/0421—Moving fluids with specific forces or mechanical means specific forces electrical forces, e.g. electrokinetic electrophoretic flow
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1006—Investigating individual particles for cytology
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N2015/1481—Optical analysis of particles within droplets

Landscapes

  • Chemical & Material Sciences (AREA)
  • Dispersion Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Hematology (AREA)
  • General Engineering & Computer Science (AREA)
  • Mechanical Engineering (AREA)
  • Clinical Laboratory Science (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Physics & Mathematics (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Signal Processing (AREA)
  • Automatic Analysis And Handling Materials Therefor (AREA)
FR0854745A 2008-07-11 2008-07-11 Procede et dispositif de manipulation et d'observation de gouttes de liquide Active FR2933713B1 (fr)

Priority Applications (4)

Application Number Priority Date Filing Date Title
FR0854745A FR2933713B1 (fr) 2008-07-11 2008-07-11 Procede et dispositif de manipulation et d'observation de gouttes de liquide
EP09793960A EP2318136A1 (fr) 2008-07-11 2009-07-09 Procede et dispositif de manipulation et d'observation de gouttes de liquide
US13/003,105 US20110147215A1 (en) 2008-07-11 2009-07-09 Method and device for manipulating and observing liquid droplets
PCT/EP2009/058777 WO2010004014A1 (fr) 2008-07-11 2009-07-09 Procede et dispositif de manipulation et d'observation de gouttes de liquide

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
FR0854745A FR2933713B1 (fr) 2008-07-11 2008-07-11 Procede et dispositif de manipulation et d'observation de gouttes de liquide

Publications (2)

Publication Number Publication Date
FR2933713A1 FR2933713A1 (fr) 2010-01-15
FR2933713B1 true FR2933713B1 (fr) 2011-03-25

Family

ID=40380456

Family Applications (1)

Application Number Title Priority Date Filing Date
FR0854745A Active FR2933713B1 (fr) 2008-07-11 2008-07-11 Procede et dispositif de manipulation et d'observation de gouttes de liquide

Country Status (4)

Country Link
US (1) US20110147215A1 (fr)
EP (1) EP2318136A1 (fr)
FR (1) FR2933713B1 (fr)
WO (1) WO2010004014A1 (fr)

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Publication number Priority date Publication date Assignee Title
CA2594483C (fr) 2005-01-28 2014-08-26 Duke University Appareils et procedes de manipulation de gouttelettes sur une carte de circuits imprimes
FR2887305B1 (fr) 2005-06-17 2011-05-27 Commissariat Energie Atomique Dispositif de pompage par electromouillage et application aux mesures d'activite electrique
US20140193807A1 (en) 2006-04-18 2014-07-10 Advanced Liquid Logic, Inc. Bead manipulation techniques
US8637324B2 (en) 2006-04-18 2014-01-28 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US8809068B2 (en) 2006-04-18 2014-08-19 Advanced Liquid Logic, Inc. Manipulation of beads in droplets and methods for manipulating droplets
US8658111B2 (en) 2006-04-18 2014-02-25 Advanced Liquid Logic, Inc. Droplet actuators, modified fluids and methods
US10078078B2 (en) 2006-04-18 2018-09-18 Advanced Liquid Logic, Inc. Bead incubation and washing on a droplet actuator
US7439014B2 (en) 2006-04-18 2008-10-21 Advanced Liquid Logic, Inc. Droplet-based surface modification and washing
WO2007123908A2 (fr) 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Opérations en puits multiples à base de gouttelettes
US8179216B2 (en) * 2006-06-06 2012-05-15 University Of Virginia Patent Foundation Capillary force actuator device and related method of applications
WO2008091848A2 (fr) 2007-01-22 2008-07-31 Advanced Liquid Logic, Inc. Chargement de fluide assisté en surface et distribution de gouttelette
EP2573562A3 (fr) 2007-02-09 2013-10-30 Advanced Liquid Logic, Inc. Dispositifs actionneurs de gouttelettes et procédés utilisant des billes magnétiques
WO2008101194A2 (fr) 2007-02-15 2008-08-21 Advanced Liquid Logic, Inc. Détection de capacité sur un actuateur goutte
WO2011084703A2 (fr) 2009-12-21 2011-07-14 Advanced Liquid Logic, Inc. Analyses d'enzymes sur un diffuseur à gouttelettes
US8702938B2 (en) 2007-09-04 2014-04-22 Advanced Liquid Logic, Inc. Droplet actuator with improved top substrate
KR20100100974A (ko) 2007-12-23 2010-09-15 어드밴스드 리퀴드 로직, 아이엔씨. 액적 작업들을 수행하는 액적 작동기 구성부 및 방법
US8926065B2 (en) 2009-08-14 2015-01-06 Advanced Liquid Logic, Inc. Droplet actuator devices and methods
US9091649B2 (en) 2009-11-06 2015-07-28 Advanced Liquid Logic, Inc. Integrated droplet actuator for gel; electrophoresis and molecular analysis
US9248450B2 (en) 2010-03-30 2016-02-02 Advanced Liquid Logic, Inc. Droplet operations platform
WO2012012090A2 (fr) 2010-06-30 2012-01-26 Advanced Liquid Logic, Inc. Ensembles actionneurs à gouttelettes et leurs procédés de fabrication
FR2967148B1 (fr) * 2010-11-10 2012-12-21 Commissariat Energie Atomique Procede d'evaporation controlee d'une goutte liquide dans un dispositif microfluidique.
WO2012085728A1 (fr) * 2010-12-20 2012-06-28 Koninklijke Philips Electronics N.V. Dispositif microfluidique comprenant un moyen de commande du flux de fluide
US9188615B2 (en) 2011-05-09 2015-11-17 Advanced Liquid Logic, Inc. Microfluidic feedback using impedance detection
CA2833907A1 (fr) 2011-05-10 2012-11-15 Advanced Liquid Logic, Inc. Concentration d'enzymes et dosages
FR2975491B1 (fr) * 2011-05-19 2013-07-05 Commissariat Energie Atomique Procede d'identification de la phase d'un echantillon fluide dans un dispositif microfluidique.
AU2012279420A1 (en) 2011-07-06 2014-01-30 Advanced Liquid Logic Inc Reagent storage on a droplet actuator
US8901043B2 (en) 2011-07-06 2014-12-02 Advanced Liquid Logic, Inc. Systems for and methods of hybrid pyrosequencing
WO2013009927A2 (fr) 2011-07-11 2013-01-17 Advanced Liquid Logic, Inc. Actionneurs de gouttelettes et techniques pour dosages à base de gouttelettes
US9446404B2 (en) 2011-07-25 2016-09-20 Advanced Liquid Logic, Inc. Droplet actuator apparatus and system
CA2854023A1 (fr) 2011-11-07 2013-05-16 Illumina, Inc. Appareils de sequencage integre et procedes d'utilisation
WO2013078216A1 (fr) 2011-11-21 2013-05-30 Advanced Liquid Logic Inc Dosages de la glucose-6-phosphate déshydrogénase
KR101337131B1 (ko) * 2012-04-18 2013-12-05 명지대학교 산학협력단 전기습윤 현상을 이용한 다중 버블 제어기술 및 이를 이용한 미소물체 제어방법
KR101923052B1 (ko) 2012-05-09 2018-11-29 리쿠아비스타 비.브이. 전기습윤 표시장치
US9223317B2 (en) 2012-06-14 2015-12-29 Advanced Liquid Logic, Inc. Droplet actuators that include molecular barrier coatings
JP6222671B2 (ja) 2012-06-27 2017-11-01 アドバンスト リキッド ロジック インコーポレイテッドＡｄｖａｎｃｅｄ Ｌｉｑｕｉｄ Ｌｏｇｉｃ， Ｉｎｃ． 泡形成を低減するための技術および液滴アクチュエーターの設計
US9863913B2 (en) 2012-10-15 2018-01-09 Advanced Liquid Logic, Inc. Digital microfluidics cartridge and system for operating a flow cell
SG11201912282YA (en) 2017-06-21 2020-01-30 Base4 Innovation Ltd Microdroplet manipulation device
AU2018288533B2 (en) 2017-06-21 2023-07-13 Lightcast Discovery Ltd Microfluidic analytical device
US20190262829A1 (en) * 2018-02-28 2019-08-29 Volta Labs, Inc. Directing Motion of Droplets Using Differential Wetting
US10913067B2 (en) * 2018-10-01 2021-02-09 Sharp Life Science (Eu) Limited Barrier droplet configurations against migration between droplets on AM-EWOD devices
EP3656473A1 (fr) * 2018-11-20 2020-05-27 Lightcast Discovery Limited Détection de microgouttelettes de cellules
GB201909514D0 (en) 2018-11-20 2019-08-14 Lightcast Discovery Ltd Device and method for microdroplet detection of cells
JP7094398B2 (ja) * 2019-02-08 2022-07-01 平田機工株式会社 標本作製方法

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FI980874A7 (fi) * 1998-04-20 1999-10-21 Wallac Oy Menetelmä ja laite pienten nestemäärien kemiallisen analyysin suorittamiseksi
US6773566B2 (en) * 2000-08-31 2004-08-10 Nanolytics, Inc. Electrostatic actuators for microfluidics and methods for using same
US6911132B2 (en) * 2002-09-24 2005-06-28 Duke University Apparatus for manipulating droplets by electrowetting-based techniques
FR2872438B1 (fr) * 2004-07-01 2006-09-15 Commissariat Energie Atomique Dispositif de deplacement et de traitement de volumes de liquide
US8129123B2 (en) * 2004-10-05 2012-03-06 The Regents Of The University Of California Methods for assessing atherogenesis by determining oxidized phospholipid to apolipoprotein B ratios
FR2887030B1 (fr) * 2005-06-09 2008-06-13 Commissariat Energie Atomique Dispositif planaire avec adressage de puits automatise par electromouillage dynamique
FR2887705B1 (fr) * 2005-06-27 2007-08-10 Commissariat Energie Atomique Dispositif de pompage ou de centrifugation des gouttes deplacees par electromouillage
US8293524B2 (en) * 2006-03-31 2012-10-23 Fluxion Biosciences Inc. Methods and apparatus for the manipulation of particle suspensions and testing thereof
WO2007123908A2 (fr) * 2006-04-18 2007-11-01 Advanced Liquid Logic, Inc. Opérations en puits multiples à base de gouttelettes

Also Published As

Publication number Publication date
US20110147215A1 (en) 2011-06-23
EP2318136A1 (fr) 2011-05-11
FR2933713A1 (fr) 2010-01-15
WO2010004014A1 (fr) 2010-01-14

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