BRPI0810357A2 - Método para aumentar a secreção de uma poi a partir de uma célula eucariótica, uso de uma sequência de nucleotídeo, sequência de nucleotídeo, sequência promotora de levedura, vetor de expressão eucariótico, e, usos de um vetor de expressão e de uma sequência promotora de levedura. - Google Patents

Método para aumentar a secreção de uma poi a partir de uma célula eucariótica, uso de uma sequência de nucleotídeo, sequência de nucleotídeo, sequência promotora de levedura, vetor de expressão eucariótico, e, usos de um vetor de expressão e de uma sequência promotora de levedura.

Info

Publication number
BRPI0810357A2
BRPI0810357A2 BRPI0810357-7A2A BRPI0810357A BRPI0810357A2 BR PI0810357 A2 BRPI0810357 A2 BR PI0810357A2 BR PI0810357 A BRPI0810357 A BR PI0810357A BR PI0810357 A2 BRPI0810357 A2 BR PI0810357A2
Authority
BR
Brazil
Prior art keywords
nucleotide sequence
yeast
sequence
eukaryotic
poi
Prior art date
Application number
BRPI0810357-7A2A
Other languages
English (en)
Inventor
Brigitte Gasser
Diethard Mattanovich
Michael Sauer
Gerhard Stadlmayr
Original Assignee
Polymun Scient Immunbio Forsch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Polymun Scient Immunbio Forsch filed Critical Polymun Scient Immunbio Forsch
Publication of BRPI0810357A2 publication Critical patent/BRPI0810357A2/pt

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Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K14/00—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof
    • C07K14/37—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi
    • C07K14/39—Peptides having more than 20 amino acids; Gastrins; Somatostatins; Melanotropins; Derivatives thereof from fungi from yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/14—Fungi; Culture media therefor
    • C12N1/16—Yeasts; Culture media therefor
    • C12N1/18—Baker's yeast; Brewer's yeast
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/11—DNA or RNA fragments; Modified forms thereof; Non-coding nucleic acids having a biological activity
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80—Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815—Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P21/00—Preparation of peptides or proteins
    • C12P21/02—Preparation of peptides or proteins having a known sequence of two or more amino acids, e.g. glutathione

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  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical & Material Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Organic Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Mycology (AREA)
  • Biotechnology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biomedical Technology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • Physics & Mathematics (AREA)
  • Plant Pathology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Medicinal Chemistry (AREA)
  • General Chemical & Material Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Gastroenterology & Hepatology (AREA)
  • Botany (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Virology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0810357-7A2A 2007-04-20 2008-04-17 Método para aumentar a secreção de uma poi a partir de uma célula eucariótica, uso de uma sequência de nucleotídeo, sequência de nucleotídeo, sequência promotora de levedura, vetor de expressão eucariótico, e, usos de um vetor de expressão e de uma sequência promotora de levedura. BRPI0810357A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
EP07008051 2007-04-20
PCT/EP2008/003076 WO2008128701A2 (en) 2007-04-20 2008-04-17 Yeast expression systems

Publications (1)

Publication Number Publication Date
BRPI0810357A2 true BRPI0810357A2 (pt) 2014-10-07

Family

ID=39800700

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0810357-7A2A BRPI0810357A2 (pt) 2007-04-20 2008-04-17 Método para aumentar a secreção de uma poi a partir de uma célula eucariótica, uso de uma sequência de nucleotídeo, sequência de nucleotídeo, sequência promotora de levedura, vetor de expressão eucariótico, e, usos de um vetor de expressão e de uma sequência promotora de levedura.

Country Status (10)

Country Link
US (1) US20100297738A1 (pt)
EP (1) EP2140008A2 (pt)
JP (1) JP2010524440A (pt)
KR (1) KR20100016170A (pt)
CN (1) CN101679992A (pt)
AU (1) AU2008241061A1 (pt)
BR (1) BRPI0810357A2 (pt)
CA (1) CA2684650A1 (pt)
EA (1) EA017803B1 (pt)
WO (1) WO2008128701A2 (pt)

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EP2258854A1 (en) * 2009-05-20 2010-12-08 FH Campus Wien Eukaryotic host cell comprising an expression enhancer
EP2258855A1 (en) 2009-05-28 2010-12-08 Universität für Bodenkultur Wien Expression sequences
WO2012109220A2 (en) * 2011-02-08 2012-08-16 Merck Sharp & Dohme Corp. Cell cycle control for improving process performance and recombinant expression in fungal host cells
KR102128957B1 (ko) 2011-10-07 2020-07-02 론자 리미티드 조절가능한 프로모터
EP3130598B1 (en) 2012-10-29 2020-02-12 Lonza Ltd Expression sequences
CN102977206B (zh) * 2012-11-19 2014-10-01 中国农业科学院生物技术研究所 细胞色素结合结构域蛋白作为助分泌因子提高外源基因在毕赤酵母中分泌表达量的用途
CN102994541B (zh) * 2012-12-19 2015-04-15 江南大学 一种共表达upr关键基因和下游靶基因增强葡萄糖氧化酶分泌的方法
BR112015021753B1 (pt) 2013-03-08 2023-02-23 Keck Graduate Institute Of Applied Life Sciences Ácidos nucleicos isolados de pichia pastoris, vetor de expressão, células hospedeiras de levedura e construto de dna, bem como método para produzir uma proteína
DK2964775T3 (en) 2013-03-08 2019-04-15 Biogrammatics Inc Yeast promoters for protein expression
US10865416B2 (en) 2014-04-17 2020-12-15 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell engineered to overexpress helper proteins
BR112016023769A2 (pt) 2014-04-17 2017-10-17 Boehringer Ingelheim Rcv Gmbh célula hospedeira recombinante para a expressão de proteínas de interesse
EP2952584A1 (en) 2014-06-04 2015-12-09 Boehringer Ingelheim RCV GmbH & Co KG Improved protein production
CN104357416A (zh) * 2014-10-22 2015-02-18 江南大学 一种改造蛋白折叠分泌途径增强葡萄糖氧化酶分泌的方法
RU2683549C1 (ru) * 2015-12-29 2019-03-28 Федеральное государственное бюджетное учреждение науки институт биоорганической химии им. академиков М.М. Шемякина и Ю.А. Овчинникова Российской академии наук (ИБХ РАН) СИСТЕМА ДЛЯ ЭКСПРЕССИИ FAB-ФРАГМЕНТОВ АНТИТЕЛ В МЕТИЛОТРОФНЫХ ДРОЖЖАХ PICHIAPASTORIS, НА ОСНОВЕ РЕКОМБИНАНТНЫХ ПЛАЗМИДНЫХ ДНК Ab-HCh-HIS/pPICZ_α_A И Ab-LCh-LAMBDA/pPICZα_A, ПРЕДНАЗНАЧЕННЫХ ДЛЯ КЛОНИРОВАНИЯ ВАРИАБЕЛЬНЫХ ДОМЕНОВ ТЯЖЕЛОЙ И ЛЕГКОЙ ЦЕПЕЙ АНТИТЕЛ, СООТВЕТСТВЕННО
CN105802867B (zh) * 2016-05-23 2019-09-17 江南大学 一种碱性果胶酶分泌增强型菌株及其应用
US11479798B2 (en) 2017-03-29 2022-10-25 Boehringer Ingelheim Rcv Gmbh & Co Kg Recombinant host cell with altered membrane lipid composition
WO2018213343A1 (en) * 2017-05-16 2018-11-22 The Regents Of The University Of California Fluorescence detection in yeast colonies
EP3630940A1 (en) 2017-05-31 2020-04-08 Universität für Bodenkultur Wien Yeast expressing a synthetic calvin cycle
CN107043757B (zh) * 2017-06-01 2020-07-07 江苏师范大学 一株异源高效表达米黑根毛霉脂肪酶的重组毕赤酵母及其应用
FR3068368A1 (fr) * 2017-06-30 2019-01-04 Compagnie Generale Des Etablissements Michelin Utilisation des polyketide synthases de type iii de bacteries comme phloroglucinol synthases
BR112020016258A2 (pt) 2018-02-12 2020-12-15 Lonza Ltd Uma célula hospedeira eucariótica modificada geneticamente projetada para reduzir a produção de proteínas da célula hospedeira, método de produzir uma proteína de interesse usando a célula hospedeira, método para reduzir a contaminação por proteína da célula hospedeira
EP3536784A1 (en) 2018-03-05 2019-09-11 ACIB GmbH Host cell engineered for improved metabolite production
SG11202012529VA (en) 2018-06-27 2021-01-28 Boehringer Ingelheim Rcv Gmbh Means and methods for increased protein expression by use of transcription factors
CN113056554A (zh) * 2018-11-19 2021-06-29 巴斯夫欧洲公司 重组酵母细胞
CN115838754B (zh) * 2018-12-27 2026-06-26 中国医学科学院药物研究所 生产达玛烯二醇-ii糖苷的重组菌及其应用
ES2921137T5 (en) 2019-01-11 2025-05-06 Lonza Ag Carbon-source regulated protein production in a recombinant host cell
WO2020200414A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Protein production in mut-methylotrophic yeast
WO2020200415A1 (en) 2019-04-01 2020-10-08 Lonza Ltd Mut- methylotrophic yeast
US12270034B2 (en) 2019-04-01 2025-04-08 Universität Für Bodenkultur Wien Mut- methylotrophic yeast
WO2021014470A1 (en) * 2019-07-25 2021-01-28 Council Of Scientific And Industrial Research Recombinant vector for high-expression of proteins in yeasts
CN110592090A (zh) * 2019-10-30 2019-12-20 福建师范大学 Ssa4基因启动子及利用该启动子驱动外源基因转录的毕赤酵母表达载体
RU2728033C1 (ru) * 2019-12-11 2020-07-28 Федеральное государственное бюджетное учреждение "Государственный научно-исследовательский институт генетики и селекции промышленных микроорганизмов национального исследовательского центра "Курчатовский институт" (НИЦ "Курчатовский институт"-ГосНИИгенетика) Трансформант дрожжей Pichia pastoris, продуцирующий эндо-1,4-β-ксиланазу из Paenibacillus brasilensis
ES3073336T3 (en) * 2020-02-13 2026-07-10 Univ Danmarks Tekniske Burden-addicted production strains
ES3034258T3 (en) 2020-04-01 2025-08-14 Lonza Ag Helper factors for expressing proteins in yeast
KR20230075436A (ko) 2020-09-30 2023-05-31 론자 리미티드 번역 인자를 과발현하는 숙주 세포
CN112280700B (zh) * 2020-10-19 2022-09-06 中国石油化工股份有限公司 一株耐乙酸和甲酸的发酵菌株及其构建方法
EP4291659A1 (en) 2021-02-12 2023-12-20 Boehringer Ingelheim RCV GmbH & Co KG Signal peptides for increased protein secretion
CN113088533B (zh) * 2021-04-15 2023-03-24 华中科技大学 一种高效表达藤壶粘胶蛋白的酵母工程菌及其制备方法
CN114657197B (zh) * 2022-04-06 2023-07-21 暨南大学 Gsm1p作为正调控因子在提高宿主细胞中蛋白表达中的应用
CN114657190B (zh) * 2022-04-06 2023-08-29 暨南大学 Msn2p作为负调控因子在提高宿主细胞中蛋白表达中的应用
WO2024126811A1 (en) 2022-12-16 2024-06-20 Boehringer Ingelheim Rcv Gmbh & Co Kg Means and methods for increased protein expression by use of a combination of transport proteins and either chaperones or transcription factors
EP4638756A1 (en) 2022-12-20 2025-10-29 Lonza Ltd. Host cells with pat1 knockout for increased specific protein productivity
CN116970503B (zh) * 2023-07-25 2024-08-02 江南大学 一种强化囊泡转运的产乳铁蛋白毕赤酵母及促进胞外分泌的方法
CN117467695B (zh) * 2023-12-27 2024-05-03 南京鸿瑞杰生物医疗科技有限公司 过表达毕赤酵母分子伴侣提高报告蛋白分泌的方法
WO2026027251A1 (en) 2024-08-01 2026-02-05 Boehringer Ingelheim Rcv Gmbh & Co Kg Signal peptides for increased protein secretion
CN119464103B (zh) * 2025-01-17 2025-05-09 上海昌进生物科技有限公司 高效表达β-乳球蛋白的菌株及其构建方法和应用
CN120574696B (zh) * 2025-05-28 2026-02-17 引加(上海)生物医药科技有限公司 一种高表达梭菌蛋白酶的基因工程菌及其应用
CN121736923A (zh) * 2026-03-02 2026-03-27 中国科学院天津工业生物技术研究所 一种在毕赤酵母中表达曲妥珠单抗的方法

Also Published As

Publication number Publication date
AU2008241061A1 (en) 2008-10-30
CN101679992A (zh) 2010-03-24
EA200970985A1 (ru) 2010-04-30
US20100297738A1 (en) 2010-11-25
CA2684650A1 (en) 2008-10-30
EA017803B1 (ru) 2013-03-29
EP2140008A2 (en) 2010-01-06
JP2010524440A (ja) 2010-07-22
WO2008128701A2 (en) 2008-10-30
KR20100016170A (ko) 2010-02-12
WO2008128701A3 (en) 2009-03-12

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