BRPI0812091A2 - Método para fabricar uma proteína policlonal recombinante - Google Patents

Método para fabricar uma proteína policlonal recombinante

Info

Publication number
BRPI0812091A2
BRPI0812091A2 BRPI0812091-9A2A BRPI0812091A BRPI0812091A2 BR PI0812091 A2 BRPI0812091 A2 BR PI0812091A2 BR PI0812091 A BRPI0812091 A BR PI0812091A BR PI0812091 A2 BRPI0812091 A2 BR PI0812091A2
Authority
BR
Brazil
Prior art keywords
collection
cells
manufacturing
recombinant polyclonal
polyclonal protein
Prior art date
Application number
BRPI0812091-9A2A
Other languages
English (en)
Inventor
Lars Soegaard Nielsen
Dietmar Weilguny
Anne Bondgaard Tolstrup
Finn C Wiberg
Christian Mueller
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Publication of BRPI0812091A2 publication Critical patent/BRPI0812091A2/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Cell Biology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
BRPI0812091-9A2A 2007-05-25 2008-05-21 Método para fabricar uma proteína policlonal recombinante BRPI0812091A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
DKPA200701292 2007-09-07
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
BRPI0812091A2 true BRPI0812091A2 (pt) 2014-10-14

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0812091-9A2A BRPI0812091A2 (pt) 2007-05-25 2008-05-21 Método para fabricar uma proteína policlonal recombinante

Country Status (22)

Country Link
US (1) US7910332B2 (pt)
EP (1) EP2152872B1 (pt)
JP (2) JP5941616B2 (pt)
KR (1) KR101555658B1 (pt)
CN (1) CN101688204B (pt)
AT (1) ATE481483T1 (pt)
AU (1) AU2008255383B2 (pt)
BR (1) BRPI0812091A2 (pt)
CA (1) CA2683800C (pt)
CY (1) CY1110939T1 (pt)
DE (1) DE602008002593D1 (pt)
DK (1) DK2152872T3 (pt)
HR (1) HRP20100640T1 (pt)
IL (1) IL201395A (pt)
MX (1) MX2009012526A (pt)
NZ (1) NZ581316A (pt)
PL (1) PL2152872T3 (pt)
PT (1) PT2152872E (pt)
SI (1) SI2152872T1 (pt)
TW (1) TW200902548A (pt)
WO (1) WO2008145133A2 (pt)
ZA (1) ZA200908021B (pt)

Families Citing this family (29)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2512647C (en) 2003-01-07 2013-10-08 Symphogen A/S Method for manufacturing recombinant polyclonal proteins
JP5669397B2 (ja) * 2007-03-01 2015-02-12 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ 同族抗体をクローニングするための方法
HRP20100640T1 (hr) * 2007-05-25 2010-12-31 Symphogen A/S Postupak proizvodnje rekombinantnog poliklonskog proteina
RU2010113510A (ru) * 2007-09-07 2011-10-20 Симфоген А/С (Dk) Способы рекомбинантной продукции антител против респираторно-цинцитиального вируса (rsv)
NZ586957A (en) * 2008-02-01 2014-03-28 Chromocell Corp Cell lines and methods for making and using them
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
CA2722348A1 (en) * 2008-04-23 2009-10-29 Symphogen A/S Methods for manufacturing a polyclonal protein
CN102112622B (zh) * 2008-05-28 2014-12-10 拜耳医药保健有限公司 用于在表达HBx的哺乳动物细胞中生产重组蛋白的方法和组合物
WO2010022738A1 (en) * 2008-08-29 2010-03-04 Symphogen A/S Method for cloning avian-derived antibodies
EP2393927B1 (en) 2009-02-09 2014-12-31 MorphoSys AG Production of oligoclonal mixtures of immunoglobulins in single cells
KR101862832B1 (ko) 2010-11-01 2018-05-30 심포젠 에이/에스 Ｐａｎ―ｈｅｒ 항체 조성물
DK2640743T3 (en) 2010-11-16 2017-01-23 Excelimmune Inc PROCEDURES FOR THE PREPARATION OF RECOMBINANT PROTEINS
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
HK1202431A1 (en) 2012-05-02 2015-10-02 Symphogen A/S Humanized pan-her antibody compositions
EP2953970A4 (en) 2013-02-08 2016-06-29 Misfolding Diagnostics Inc TRANSTYRETIN ANTIBODIES AND USES THEREOF
ES2748295T3 (es) 2014-09-16 2020-03-16 Symphogen As Anticuerpos anti-MET y composiciones
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
US20190024114A1 (en) * 2016-01-14 2019-01-24 Anton Bauer Method of producing a production cell line
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
KR102737715B1 (ko) 2017-04-05 2024-12-03 르 라보레또레 쎄르비에르 Pd-1, tim-3 및 lag-3을 표적화하는 조합 요법
EP3810649A1 (en) * 2018-06-22 2021-04-28 Genmab A/S Method for producing a controlled mixture of two or more different antibodies
KR102559149B1 (ko) 2018-10-26 2023-07-24 에프. 호프만-라 로슈 아게 재조합효소 매개 카세트 교환을 이용한 다중특이적 항체 스크리닝 방법
US12016885B2 (en) 2018-11-06 2024-06-25 Alsatech, Inc. Method of solubilizing TDP-43 protein aggregates in amyotrophic lateral sclerosis using a microvascular endothelial cell expressing an anti-TDP-43 Fab
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
JP2022531113A (ja) * 2019-04-30 2022-07-06 ギガジェン，インコーポレイティッド 組換えポリクローナルタンパク質およびその使用方法
US20220344000A1 (en) * 2019-09-27 2022-10-27 Glaxosmithkline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines
CA3177152A1 (en) 2020-06-12 2021-12-16 David Scott Johnson Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
IL324556A (en) 2023-05-19 2026-01-01 Servier Lab Anti-met antibodies, antibody-drug conjugates, compositions and uses thereof

Family Cites Families (17)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
EP0530216A4 (en) 1990-04-19 1993-06-30 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
EP1293564A4 (en) * 2000-06-08 2005-08-17 Mitsubishi Pharma Corp EXPRESSION VECTOR FOR SCREENING CELLS HIGH EXPRESSION OF RECOMBINANT PROTEINS, TRANSFORMING HIGH EXPRESSION OF A RECOMBINANT PROTEIN AND USE THEREOF
US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
EP2314629B2 (en) * 2002-07-18 2022-11-16 Merus N.V. Recombinant production of mixtures of antibodies
AU2003283995A1 (en) 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
CA2512647C (en) * 2003-01-07 2013-10-08 Symphogen A/S Method for manufacturing recombinant polyclonal proteins
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
MX2007000644A (es) * 2004-07-20 2007-03-28 Symphogen As Anticuerpo policlonal recombinante anti-rhesus d y metodos de fabricacion.
PL1787126T3 (pl) * 2004-07-20 2010-03-31 Symphogen As Sposób charakteryzowania poliklonalnej linii komórkowej
JP2009518320A (ja) 2005-12-05 2009-05-07 シュムフォウエン アクティーゼルスカブ 抗オルトポックスウイルス組換えポリクローナル抗体
MX2008011280A (es) 2006-03-06 2008-09-12 Symphogen As Anticuerpor policlonal recombinante para el tratamiento de infecciones por el virus sincitial respiratorio.
HRP20100640T1 (hr) * 2007-05-25 2010-12-31 Symphogen A/S Postupak proizvodnje rekombinantnog poliklonskog proteina

Also Published As

Publication number Publication date
KR20100017923A (ko) 2010-02-16
AU2008255383B2 (en) 2012-11-01
TW200902548A (en) 2009-01-16
HRP20100640T1 (hr) 2010-12-31
PL2152872T3 (pl) 2011-03-31
KR101555658B1 (ko) 2015-09-24
HK1134519A1 (en) 2010-04-30
WO2008145133A3 (en) 2009-02-05
ATE481483T1 (de) 2010-10-15
WO2008145133A2 (en) 2008-12-04
EP2152872B1 (en) 2010-09-15
IL201395A0 (en) 2011-08-01
JP5941616B2 (ja) 2016-06-29
IL201395A (en) 2015-09-24
CN101688204A (zh) 2010-03-31
US20090111142A1 (en) 2009-04-30
PT2152872E (pt) 2010-11-17
CN101688204B (zh) 2013-07-03
EP2152872A2 (en) 2010-02-17
MX2009012526A (es) 2009-12-09
CA2683800A1 (en) 2008-12-04
CY1110939T1 (el) 2015-06-10
DK2152872T3 (da) 2010-12-06
AU2008255383A1 (en) 2008-12-04
JP2015037411A (ja) 2015-02-26
JP2011504721A (ja) 2011-02-17
DE602008002593D1 (de) 2010-10-28
CA2683800C (en) 2015-03-17
US7910332B2 (en) 2011-03-22
NZ581316A (en) 2012-02-24
SI2152872T1 (sl) 2011-01-31
ZA200908021B (en) 2010-11-24

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Legal Events

Date Code Title Description
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11E Dismissal acc. art. 34 of ipl - requirements for examination incomplete
B11T Dismissal of application maintained [chapter 11.20 patent gazette]
B350 Update of information on the portal [chapter 15.35 patent gazette]