DK2152872T3 - Fremgangsmåde til fremstilling af et rekombinant polyklonalt protein - Google Patents

Fremgangsmåde til fremstilling af et rekombinant polyklonalt protein

Info

Publication number
DK2152872T3
DK2152872T3 DK08748815.1T DK08748815T DK2152872T3 DK 2152872 T3 DK2152872 T3 DK 2152872T3 DK 08748815 T DK08748815 T DK 08748815T DK 2152872 T3 DK2152872 T3 DK 2152872T3
Authority
DK
Denmark
Prior art keywords
collection
cells
recombinant polyclonal
polyclonal protein
producing
Prior art date
Application number
DK08748815.1T
Other languages
English (en)
Inventor
Lars Soegaard Nielsen
Anne Bondgaard Tolstrup
Finn Wiberg
Christian Mueller
Dietmar Weilguny
Original Assignee
Symphogen As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Symphogen As filed Critical Symphogen As
Application granted granted Critical
Publication of DK2152872T3 publication Critical patent/DK2152872T3/da

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/10—Processes for the isolation, preparation or purification of DNA or RNA
    • C12N15/1034—Isolating an individual clone by screening libraries
    • C12N15/1086—Preparation or screening of expression libraries, e.g. reporter assays
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K16/00—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies
    • C07K16/08—Immunoglobulins [IG], e.g. monoclonal or polyclonal antibodies against material from viruses
    • C07K16/081—DNA viruses
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N5/00—Undifferentiated human, animal or plant cells, e.g. cell lines; Tissues; Cultivation or maintenance thereof; Culture media therefor
    • C12N5/10—Cells modified by introduction of foreign genetic material

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Genetics & Genomics (AREA)
  • Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biotechnology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Wood Science & Technology (AREA)
  • Biomedical Technology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • General Engineering & Computer Science (AREA)
  • Medicinal Chemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Physics & Mathematics (AREA)
  • Virology (AREA)
  • Plant Pathology (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Crystallography & Structural Chemistry (AREA)
  • Cell Biology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Peptides Or Proteins (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)
  • Non-Volatile Memory (AREA)
DK08748815.1T 2007-05-25 2008-05-21 Fremgangsmåde til fremstilling af et rekombinant polyklonalt protein DK2152872T3 (da)

Applications Claiming Priority (5)

Application Number Priority Date Filing Date Title
DKPA200700764 2007-05-25
US92470807P 2007-05-29 2007-05-29
DKPA200701292 2007-09-07
US96000207P 2007-09-11 2007-09-11
PCT/DK2008/050116 WO2008145133A2 (en) 2007-05-25 2008-05-21 Method for manufacturing a recombinant polyclonal protein

Publications (1)

Publication Number Publication Date
DK2152872T3 true DK2152872T3 (da) 2010-12-06

Family

ID=39739965

Family Applications (1)

Application Number Title Priority Date Filing Date
DK08748815.1T DK2152872T3 (da) 2007-05-25 2008-05-21 Fremgangsmåde til fremstilling af et rekombinant polyklonalt protein

Country Status (22)

Country Link
US (1) US7910332B2 (da)
EP (1) EP2152872B1 (da)
JP (2) JP5941616B2 (da)
KR (1) KR101555658B1 (da)
CN (1) CN101688204B (da)
AT (1) ATE481483T1 (da)
AU (1) AU2008255383B2 (da)
BR (1) BRPI0812091A2 (da)
CA (1) CA2683800C (da)
CY (1) CY1110939T1 (da)
DE (1) DE602008002593D1 (da)
DK (1) DK2152872T3 (da)
HR (1) HRP20100640T1 (da)
IL (1) IL201395A (da)
MX (1) MX2009012526A (da)
NZ (1) NZ581316A (da)
PL (1) PL2152872T3 (da)
PT (1) PT2152872E (da)
SI (1) SI2152872T1 (da)
TW (1) TW200902548A (da)
WO (1) WO2008145133A2 (da)
ZA (1) ZA200908021B (da)

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Publication number Priority date Publication date Assignee Title
ES2273202T3 (es) 2003-01-07 2007-05-01 Symphogen A/S Procedimiento para producir proteinas policlonales recombinantes.
PT2121920E (pt) * 2007-03-01 2011-10-03 Symphogen As Método de clonagem de anticorpos cognatos
HRP20100640T1 (hr) * 2007-05-25 2010-12-31 Symphogen A/S Postupak proizvodnje rekombinantnog poliklonskog proteina
CA2695309A1 (en) * 2007-09-07 2009-03-12 Symphogen A/S Methods for recombinant manufacturing of anti-rsv antibodies
EP2245171A2 (en) * 2008-02-01 2010-11-03 Chromocell Corporation Cell lines and methods for making and using them
EP2098536A1 (en) 2008-03-05 2009-09-09 4-Antibody AG Isolation and identification of antigen- or ligand-specific binding proteins
RU2010147652A (ru) * 2008-04-23 2012-05-27 Симфоген А/С (Dk) Способы производства поликлонального белка
US8882484B2 (en) * 2008-05-28 2014-11-11 Bayer Healthcare Llc Methods and compositions for production of recombinant protein in HBX-expressing mammalian cells
RU2011111725A (ru) * 2008-08-29 2012-10-10 Симфоген А/С (Dk) Способ клонирования антител птиц
WO2010089387A1 (en) 2009-02-09 2010-08-12 Morphosys Ag Production of oligoclonal mixtures of immunoglobulins in single cells
JP6033783B2 (ja) 2010-11-01 2016-11-30 シムフォゲン・アクティーゼルスカブＳｙｍｐｈｏｇｅｎ Ａ／Ｓ Ｐａｎ−ｈｅｒ抗体組成物
DK2640743T3 (da) * 2010-11-16 2017-01-23 Excelimmune Inc Fremgangsmåder til fremstilling af rekombinante proteiner
CN102558353A (zh) * 2011-12-09 2012-07-11 上海应用技术学院 一种大肠杆菌NrfA基因表达蛋白多克隆抗体及其制备方法
GB2502127A (en) 2012-05-17 2013-11-20 Kymab Ltd Multivalent antibodies and in vivo methods for their production
EP2844675B1 (en) 2012-05-02 2019-01-02 Symphogen A/S Humanized pan-her antibody compositions
EP2953970A4 (en) 2013-02-08 2016-06-29 Misfolding Diagnostics Inc TRANSTYRETIN ANTIBODIES AND USES THEREOF
US10450376B2 (en) 2014-09-16 2019-10-22 Symphogen A/S Anti-MET antibodies and compositions
US12404552B2 (en) * 2015-07-17 2025-09-02 Allele Biotechnology & Pharmaceuticals, Inc. Methods of selecting antibodies and antibody fragments
WO2017121896A1 (en) * 2016-01-14 2017-07-20 Anton Bauer Method of producing a production cell line
WO2018073393A2 (en) 2016-10-19 2018-04-26 Cellectis Tal-effector nuclease (talen) -modified allogenic cells suitable for therapy
EA201992350A1 (ru) 2017-04-05 2020-03-23 Симфоген А/С Комбинированные лекарственные средства, нацеленные на pd-1, tim-3 и lag-3
CN112513074A (zh) * 2018-06-22 2021-03-16 健玛保 生产两种或更多种不同抗体的受控混合物的方法
EP3870604B1 (en) 2018-10-26 2022-11-23 F. Hoffmann-La Roche AG Multispecific antibody screening method using recombinase mediated cassette exchange
CA3118692A1 (en) 2018-11-06 2020-05-14 Alsatech, Inc. Cell-based gene therapy for neurodegenerative diseases
CN109988751A (zh) * 2019-01-15 2019-07-09 济宁学院 一种用于制备抗egfr全人源化单克隆抗体的细胞株构建方法
US12590386B2 (en) * 2019-04-30 2026-03-31 Gigagen, Inc. Recombinant polyclonal proteins and methods of use thereof
US20220344000A1 (en) * 2019-09-27 2022-10-27 Glaxosmithkline Intellectual Property Development Limited Method of predicting production stability of clonal cell lines
CA3177152A1 (en) 2020-06-12 2021-12-16 David Scott Johnson Recombinant polyclonal proteins targeting covid-19 and methods of use thereof
IL324556A (en) 2023-05-19 2026-01-01 Servier Lab Anti-met antibodies, antibody-drug conjugates, compositions and uses thereof

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EP0530216A4 (en) 1990-04-19 1993-06-30 Medimmune, Inc. Administration of monoclonal antibodies against respiratory viruses
GB9022543D0 (en) * 1990-10-17 1990-11-28 Wellcome Found Antibody production
US6111166A (en) 1994-09-19 2000-08-29 Medarex, Incorporated Transgenic mice expressing human Fcα and β receptors
GB9703369D0 (en) 1997-02-18 1997-04-09 Lindqvist Bjorn H Process
US20040038392A1 (en) * 2000-06-08 2004-02-26 Tatsurou Shibui Expression vector enabling screening of cells with high expression of recombinant protein, transformant with high expression of recombinant protein and utilization thereof
US6849259B2 (en) * 2000-06-16 2005-02-01 Symphogen A/S Polyclonal antibody composition for treating allergy
NZ537277A (en) * 2002-07-18 2008-04-30 Crucell Holland Bv Recombinant production of mixtures of antibodies
AU2003283995A1 (en) * 2002-09-30 2004-04-19 Protein Design Labs, Inc. Efficient generation of stable expression cell lines through the use of scorable homeostatic reporter genes
ES2273202T3 (es) * 2003-01-07 2007-05-01 Symphogen A/S Procedimiento para producir proteinas policlonales recombinantes.
CN100383244C (zh) * 2003-01-07 2008-04-23 西福根有限公司 用于生产重组多克隆蛋白质的方法
US20060275766A1 (en) * 2003-01-07 2006-12-07 Haurum John S Method for manufacturing recombinant polyclonal proteins
TWI333977B (en) * 2003-09-18 2010-12-01 Symphogen As Method for linking sequences of interest
ES2332524T3 (es) * 2004-07-20 2010-02-08 Symphogen A/S Un procedimiento para la caracterizacion de una linea celular policlonal.
KR20070038556A (ko) * 2004-07-20 2007-04-10 심포젠 에이/에스 항-ｒｈｅｓｕｓ ｄ 재조합 폴리클로날 항체 및 이의 제조방법
EP2314619A1 (en) 2005-12-05 2011-04-27 Symphogen A/S Anti-orthopoxvirus recombinant polyclonal antibody
JP2009528828A (ja) 2006-03-06 2009-08-13 シムフォゲン・アクティーゼルスカブ 消化器多核体ウイルス感染症の治療のための組み換えポリクローナル抗体
HRP20100640T1 (hr) * 2007-05-25 2010-12-31 Symphogen A/S Postupak proizvodnje rekombinantnog poliklonskog proteina

Also Published As

Publication number Publication date
PT2152872E (pt) 2010-11-17
JP2015037411A (ja) 2015-02-26
KR20100017923A (ko) 2010-02-16
MX2009012526A (es) 2009-12-09
CA2683800A1 (en) 2008-12-04
WO2008145133A2 (en) 2008-12-04
SI2152872T1 (sl) 2011-01-31
ATE481483T1 (de) 2010-10-15
CN101688204A (zh) 2010-03-31
ZA200908021B (en) 2010-11-24
PL2152872T3 (pl) 2011-03-31
HRP20100640T1 (hr) 2010-12-31
JP5941616B2 (ja) 2016-06-29
EP2152872A2 (en) 2010-02-17
TW200902548A (en) 2009-01-16
NZ581316A (en) 2012-02-24
BRPI0812091A2 (pt) 2014-10-14
WO2008145133A3 (en) 2009-02-05
JP2011504721A (ja) 2011-02-17
HK1134519A1 (en) 2010-04-30
DE602008002593D1 (de) 2010-10-28
CA2683800C (en) 2015-03-17
IL201395A0 (en) 2011-08-01
US7910332B2 (en) 2011-03-22
AU2008255383A1 (en) 2008-12-04
AU2008255383B2 (en) 2012-11-01
KR101555658B1 (ko) 2015-09-24
IL201395A (en) 2015-09-24
EP2152872B1 (en) 2010-09-15
US20090111142A1 (en) 2009-04-30
CN101688204B (zh) 2013-07-03
CY1110939T1 (el) 2015-06-10

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