BRPI0819509A2 - MICROORGANISMO E VETOR RECOMBINANTE, SACAROSE FOSFOTRANSFERASE, GENE (ptsG), BETA-FRUTOFURANOSIDASE E MÉTODO PARA PRODUZIR METABÓLITOCOS, POLÍMEROS BIODEGRADÁVEIS OU PROTEÍNAS RECOMBINANTES - Google Patents

MICROORGANISMO E VETOR RECOMBINANTE, SACAROSE FOSFOTRANSFERASE, GENE (ptsG), BETA-FRUTOFURANOSIDASE E MÉTODO PARA PRODUZIR METABÓLITOCOS, POLÍMEROS BIODEGRADÁVEIS OU PROTEÍNAS RECOMBINANTES

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Publication number
BRPI0819509A2
BRPI0819509A2 BRPI0819509-9A2A BRPI0819509A BRPI0819509A2 BR PI0819509 A2 BRPI0819509 A2 BR PI0819509A2 BR PI0819509 A BRPI0819509 A BR PI0819509A BR PI0819509 A2 BRPI0819509 A2 BR PI0819509A2
Authority
BR
Brazil
Prior art keywords
sacarosis
phospotransferase
frutofuranosidase
metabolytes
recombinable
Prior art date
Application number
BRPI0819509-9A2A
Other languages
English (en)
Inventor
Sang Yup
Hyohak Song
Ji Mahn
Sol Choi
Jin Hwan
Jeong Wook
Original Assignee
Korea Advanced Inst Sci & Tech
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Korea Advanced Inst Sci & Tech filed Critical Korea Advanced Inst Sci & Tech
Publication of BRPI0819509A2 publication Critical patent/BRPI0819509A2/pt

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    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N1/00—Microorganisms; Compositions thereof; Processes of propagating, maintaining or preserving microorganisms or compositions thereof; Processes of preparing or isolating a composition containing a microorganism; Culture media therefor
    • C12N1/20—Bacteria; Culture media therefor
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09—Recombinant DNA-technology
    • C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/70—Vectors or expression systems specially adapted for E. coli
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/10—Transferases (2.)
    • C12N9/12—Transferases (2.) transferring phosphorus containing groups, e.g. kinases (2.7)
    • C12N9/1205—Phosphotransferases with an alcohol group as acceptor (2.7.1), e.g. protein kinases
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/16—Hydrolases (3) acting on ester bonds (3.1)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00—Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14—Hydrolases (3)
    • C12N9/24—Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402—Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405—Glucanases
    • C12N9/2408—Glucanases acting on alpha -1,4-glucosidic bonds
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P1/00—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes
    • C12P1/04—Preparation of compounds or compositions, not provided for in groups C12P3/00 - C12P39/00, by using microorganisms or enzymes by using bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P13/00—Preparation of nitrogen-containing organic compounds
    • C12P13/04—Alpha- or beta- amino acids
    • C12P13/08—Lysine; Diaminopimelic acid; Threonine; Valine
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/02—Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04—Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06—Ethanol, i.e. non-beverage
    • C12P7/065—Ethanol, i.e. non-beverage with microorganisms other than yeasts
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/44—Polycarboxylic acids
    • C12P7/46—Dicarboxylic acids having four or less carbon atoms, e.g. fumaric acid, maleic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/54—Acetic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12P—FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00—Preparation of oxygen-containing organic compounds
    • C12P7/40—Preparation of oxygen-containing organic compounds containing a carboxyl group including Peroxycarboxylic acids
    • C12P7/56—Lactic acid
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01004—Fructokinase (2.7.1.4)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y207/00—Transferases transferring phosphorus-containing groups (2.7)
    • C12Y207/01—Phosphotransferases with an alcohol group as acceptor (2.7.1)
    • C12Y207/01069—Protein-Npi-phosphohistidine-sugar phosphotransferase (2.7.1.69), i.e. sucrose phosphotransferase system II
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Y—ENZYMES
    • C12Y302/00—Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01—Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01026—Beta-fructofuranosidase (3.2.1.26), i.e. invertase
    • Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02E—REDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00—Technologies for the production of fuel of non-fossil origin
    • Y02E50/10—Biofuels, e.g. bio-diesel

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  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Biotechnology (AREA)
  • Microbiology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Biomedical Technology (AREA)
  • Molecular Biology (AREA)
  • Medicinal Chemistry (AREA)
  • Virology (AREA)
  • Tropical Medicine & Parasitology (AREA)
  • Mycology (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
  • Enzymes And Modification Thereof (AREA)
BRPI0819509-9A2A 2007-12-18 2008-12-18 MICROORGANISMO E VETOR RECOMBINANTE, SACAROSE FOSFOTRANSFERASE, GENE (ptsG), BETA-FRUTOFURANOSIDASE E MÉTODO PARA PRODUZIR METABÓLITOCOS, POLÍMEROS BIODEGRADÁVEIS OU PROTEÍNAS RECOMBINANTES BRPI0819509A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
KR20070133239 2007-12-18
KR20080106113 2008-10-28
PCT/KR2008/007533 WO2009078687A2 (en) 2007-12-18 2008-12-18 Recombinant microorganism having an ability of using sucrose as a carbon source

Publications (1)

Publication Number Publication Date
BRPI0819509A2 true BRPI0819509A2 (pt) 2014-10-14

Family

ID=40796042

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0819509-9A2A BRPI0819509A2 (pt) 2007-12-18 2008-12-18 MICROORGANISMO E VETOR RECOMBINANTE, SACAROSE FOSFOTRANSFERASE, GENE (ptsG), BETA-FRUTOFURANOSIDASE E MÉTODO PARA PRODUZIR METABÓLITOCOS, POLÍMEROS BIODEGRADÁVEIS OU PROTEÍNAS RECOMBINANTES

Country Status (8)

Country Link
US (2) US20110269183A1 (pt)
EP (1) EP2227541B1 (pt)
JP (1) JP2011505869A (pt)
CN (1) CN101970648A (pt)
AU (1) AU2008339217A1 (pt)
BR (1) BRPI0819509A2 (pt)
ES (1) ES2424240T3 (pt)
WO (1) WO2009078687A2 (pt)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN102333859B (zh) * 2008-09-16 2015-04-22 三井化学株式会社 由来自植物的原料生产乳酸的方法及生产乳酸的细菌
CN102197135B (zh) 2008-11-05 2014-08-13 三井化学株式会社 生产2-脱氧蟹肌醇(doi)的细菌及使用其生产2-脱氧蟹肌醇(doi)的方法
IN2012DN02338A (pt) 2009-09-16 2015-08-21 Mitsui Chemicals Inc
DE102010029973A1 (de) * 2010-06-11 2011-12-15 Evonik Degussa Gmbh Mikrobiologische Herstellung von C4-Körpern aus Saccharose und Kohlendioxid
PH12013500068A1 (en) * 2010-07-12 2016-11-16 Inbiose Nv Metabolically engineered organisms for the production of added value bio-products
US8129170B1 (en) 2010-12-06 2012-03-06 E.I. Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
US8222000B2 (en) 2010-12-06 2012-07-17 E I Du Pont De Nemours And Company Recombinant bacteria having the ability to metabolize sucrose
CN103347999B (zh) * 2010-12-13 2016-02-10 麦兰特公司 使用含有蔗糖的原料生产琥珀酸和其他化学品的方法
AR086790A1 (es) * 2011-06-29 2014-01-22 Metabolic Explorer Sa Un microorganismo para la produccion de metionina con importacion de glucosa mejorada
US8673602B2 (en) 2011-08-16 2014-03-18 E I Du Pont De Nemours And Company Recombinant bacteria having improved sucrose utilization
US8629243B2 (en) 2011-08-16 2014-01-14 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides that enable faster sucrose utilization in bacteria
US8686114B2 (en) 2012-03-05 2014-04-01 E I Du Pont De Nemours And Company Variant sucrose transporter polypeptides
US9017961B2 (en) 2012-03-05 2015-04-28 E.I. Du Pont De Nemours And Company Recombinant bacteria comprising novel sucrose transporters
KR20140102393A (ko) * 2013-02-13 2014-08-22 씨제이제일제당 (주) L-쓰레오닌 생산능을 가지는 재조합 에스케리키아 속 미생물 및 이를 이용한 l-쓰레오닌의 생산방법
WO2016030373A1 (en) * 2014-08-28 2016-03-03 Basf Se Modified microorganism for improved production of fine chemicals on sucrose
KR101774431B1 (ko) 2016-01-28 2017-09-05 한국과학기술원 자일로즈로부터 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체 생산능을 가지는 재조합 미생물 및 이를 이용한 폴리(락테이트-co-글라이콜레이트) 또는 그 공중합체의 제조방법
WO2018008646A1 (ja) * 2016-07-06 2018-01-11 国立大学法人名古屋大学 Ｇｃｓ１改変植物種子のスクロース含有破砕液
CA3062384A1 (en) * 2017-05-19 2018-11-22 Basf Se Process for producing an organic compound
KR102187682B1 (ko) 2018-06-08 2020-12-07 한국과학기술원 타입 iii 폴리케타이드 합성 효소 기반 신규 말로닐-코에이 바이오센서 및 그 용도
CN109330590B (zh) * 2018-12-07 2022-05-27 南方科技大学 一种用于表皮信号采集的表皮电极及其应用
WO2022243311A1 (en) 2021-05-17 2022-11-24 Dsm Ip Assets B.V. Microbial strain expressing an invertase/sucrose hydrolase
CN117321071A (zh) * 2021-05-17 2023-12-29 帝斯曼知识产权资产管理有限公司 表达转化酶/蔗糖水解酶的微生物菌株
CN117074699B (zh) * 2023-10-17 2024-01-12 中国农业大学 一种筛选代谢蔗糖的干酪乳杆菌发酵剂的方法
CN119955762A (zh) * 2023-11-07 2025-05-09 廊坊梅花生物技术开发有限公司 蔗糖-6-磷酸水解酶突变的重组微生物及其在生产核苷中的应用
KR20250105844A (ko) * 2023-12-29 2025-07-09 주식회사 삼양사 케스토스를 고생산하는 변이 효소
WO2025248537A1 (en) * 2024-05-29 2025-12-04 Praj Industries Limited Engineered organism for utilizing sucrose

Family Cites Families (1)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US9297028B2 (en) * 2005-09-29 2016-03-29 Butamax Advanced Biofuels Llc Fermentive production of four carbon alcohols

Also Published As

Publication number Publication date
EP2227541B1 (en) 2013-06-19
WO2009078687A3 (en) 2009-10-15
ES2424240T3 (es) 2013-09-30
EP2227541A2 (en) 2010-09-15
EP2227541A4 (en) 2012-05-16
US20130078673A1 (en) 2013-03-28
US20110269183A1 (en) 2011-11-03
AU2008339217A1 (en) 2009-06-25
WO2009078687A2 (en) 2009-06-25
JP2011505869A (ja) 2011-03-03
CN101970648A (zh) 2011-02-09

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