BRPI0820156B8 - processo para purificação de uma biomolécula alvo - Google Patents

processo para purificação de uma biomolécula alvo

Info

Publication number
BRPI0820156B8
BRPI0820156B8 BRPI0820156A BRPI0820156A BRPI0820156B8 BR PI0820156 B8 BRPI0820156 B8 BR PI0820156B8 BR PI0820156 A BRPI0820156 A BR PI0820156A BR PI0820156 A BRPI0820156 A BR PI0820156A BR PI0820156 B8 BRPI0820156 B8 BR PI0820156B8
Authority
BR
Brazil
Prior art keywords
target biomolecule
purification
dual
affinity polypeptide
biomolecule
Prior art date
Application number
BRPI0820156A
Other languages
English (en)
Inventor
Kyhse-Andersen Jan
Original Assignee
Chreto Aps
Novozymes As
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Chreto Aps, Novozymes As filed Critical Chreto Aps
Publication of BRPI0820156A2 publication Critical patent/BRPI0820156A2/pt
Publication of BRPI0820156B1 publication Critical patent/BRPI0820156B1/pt
Publication of BRPI0820156B8 publication Critical patent/BRPI0820156B8/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C07—ORGANIC CHEMISTRY
    • C07K—PEPTIDES
    • C07K1/00—General methods for the preparation of peptides, i.e. processes for the organic chemical preparation of peptides or proteins of any length
    • C07K1/14—Extraction; Separation; Purification
    • C07K1/16—Extraction; Separation; Purification by chromatography
    • C07K1/22—Affinity chromatography or related techniques based upon selective absorption processes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Biochemistry (AREA)
  • Biophysics (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Peptides Or Proteins (AREA)
  • Solid-Sorbent Or Filter-Aiding Compositions (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

processo para purificação de uma biomolécula alvo. a presente invenção diz respeito a um processo para a purificação de uma biomolécula alvo, que compreende as etapas: (a) contatar (i) uma biomolécula alvo, (ii) um poiipeptídeo de afinidade dupla e (iii) um suporte sóiido que compreende um ligando de captura em que a razão entre as constante de dissociação de equilíbrio do polipeptideo de afinidade dupla, [kdj/kds]3 é pelo menos 10°nas condições padrão; e (b) recuperar a biomolécula alvo pela eluição.
BRPI0820156A 2007-11-12 2008-11-12 processo para purificação de uma biomolécula alvo BRPI0820156B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
EP07120454 2007-11-12
EP07120454 2007-11-12
PCT/EP2008/065346 WO2009062942A2 (en) 2007-11-12 2008-11-12 Dual affinity polypeptides for purification

Publications (3)

Publication Number Publication Date
BRPI0820156A2 BRPI0820156A2 (pt) 2015-10-20
BRPI0820156B1 BRPI0820156B1 (pt) 2021-01-12
BRPI0820156B8 true BRPI0820156B8 (pt) 2021-05-25

Family

ID=40568532

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0820156A BRPI0820156B8 (pt) 2007-11-12 2008-11-12 processo para purificação de uma biomolécula alvo

Country Status (15)

Country Link
US (2) US20110207916A1 (pt)
EP (1) EP2220107B1 (pt)
CN (1) CN101910194B (pt)
AU (1) AU2008322998B2 (pt)
BR (1) BRPI0820156B8 (pt)
CA (1) CA2705334C (pt)
DK (1) DK2220107T3 (pt)
ES (1) ES2613029T3 (pt)
HR (1) HRP20170069T1 (pt)
HU (1) HUE032930T2 (pt)
LT (1) LT2220107T (pt)
PL (1) PL2220107T3 (pt)
PT (1) PT2220107T (pt)
SI (1) SI2220107T1 (pt)
WO (1) WO2009062942A2 (pt)

Families Citing this family (20)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CN101910194B (zh) 2007-11-12 2017-03-08 科雷托公司 供纯化用的双亲和性多肽
ES2774186T3 (es) 2009-05-07 2020-07-17 Chreto Aps Método para la purificación de polipéptidos diana
KR101855714B1 (ko) 2010-03-05 2018-05-10 베링거 인겔하임 인터내셔날 게엠베하 항체의 선택적 농축
EP2436389A1 (en) * 2010-10-01 2012-04-04 Nestec S.A. Milk-based protein hydrolysates and infant formulae and nutritional compositions made thereof
CN103534349B (zh) * 2010-10-01 2017-07-21 诺维信公司 具有内肽酶活性的多肽以及编码其的多核苷酸
DK2831105T3 (da) * 2012-03-28 2017-11-20 Univ Texas Tgf-type ii-type iii-receptorfusioner
CA3204845A1 (en) 2014-08-15 2016-02-18 Ecolab Usa Inc. Cip wash summary and library
ES3032400T3 (en) * 2014-08-15 2025-07-18 Ecolab Usa Inc Cip wash comparison and simulation
CA2965862A1 (en) 2014-10-28 2016-05-06 Merck Patent Gmbh Methods for non-covalent fc-domain-containing protein display on the surface of cells and methods of screening thereof
EP3298137A1 (en) * 2015-05-22 2018-03-28 DuPont Nutrition Biosciences ApS Aldc production methods
CN108191956B (zh) * 2017-12-25 2020-10-30 浙江大学 组合型配基、组合型仿生层析介质及其制备方法和应用
CN108440666B (zh) * 2018-03-07 2021-03-23 深圳市伯劳特生物制品有限公司 一种生物素化的insulin抗原及其生物素化工艺
PL4134157T3 (pl) 2018-12-17 2025-03-03 Chreto Aps Zastosowanie podłoża z łącznikiem ligandu do oczyszczania
WO2020200941A1 (en) 2019-03-29 2020-10-08 F. Hoffmann-La Roche Ag Spr-based binding assay for the functional analysis of multivalent molecules
EP4065619A1 (en) 2019-11-25 2022-10-05 3M Innovative Properties Company Biotin-containing monomers and articles formed therefrom
CN116925197A (zh) * 2021-12-21 2023-10-24 百林科(兰州)新材料有限公司 耐碱蛋白A变体在纯化IgG抗体中的应用
US20250311734A1 (en) 2022-05-14 2025-10-09 Novozymes A/S Compositions and Methods for Preventing, Treating, Suppressing and/or Eliminatting Phytopathogenic Infestations and Infections
WO2024110444A1 (en) * 2022-11-21 2024-05-30 Chreto Aps Method for purification of a target product using affinity purification technology
CN116068206A (zh) * 2023-03-15 2023-05-05 安徽惠邦生物工程有限公司 一种髓鞘碱性蛋白检测试剂盒及其检测方法
EP4541444A1 (en) * 2023-10-20 2025-04-23 Cytiva BioProcess R&D AB Fusion protein for affinity capture

Family Cites Families (18)

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Publication number Priority date Publication date Assignee Title
AU612370B2 (en) * 1987-05-21 1991-07-11 Micromet Ag Targeted multifunctional proteins
US5169936A (en) 1989-04-14 1992-12-08 Biogen, Inc. Protein purification on immobilized metal affinity resins effected by elution using a weak ligand
US5328985A (en) * 1991-07-12 1994-07-12 The Regents Of The University Of California Recombinant streptavidin-protein chimeras useful for conjugation of molecules in the immune system
US5429746A (en) * 1994-02-22 1995-07-04 Smith Kline Beecham Corporation Antibody purification
WO1997009068A2 (en) 1995-09-01 1997-03-13 University Of Washington Interactive molecular conjugates
US6497881B1 (en) 1995-11-30 2002-12-24 New York University High efficiency tissue specific compound delivery system using streptavidin-protein a fusion protein
US6753189B1 (en) * 1998-06-04 2004-06-22 Mizuho Medy Co., Ltd. Detection apparatus and method for the same
US6831160B1 (en) 2000-01-24 2004-12-14 The Regents Of The University Of California Method of affinity purifying proteins using modified bis-arsenical fluorescein
ATE469661T1 (de) * 2000-06-16 2010-06-15 Mitra Medical Technology Ab Methode zu der konditionierung einer saüle mit avidin oder streptavidin matrix in einer vorrichtung ausserhalb des körpers
EP1529844B2 (en) 2003-07-21 2018-11-07 Fraunhofer-Gesellschaft zur Förderung der angewandten Forschung e.V. Complex formation for the stabilisation and purification of proteins of interest
US7956165B2 (en) * 2003-07-24 2011-06-07 Affisink Biotechnology Ltd. Compositions and methods for purifying and crystallizing molecules of interest
FI20031663A0 (fi) 2003-11-14 2003-11-14 Jyvaeskylaen Yliopisto Avidiinin mutantteja
CA2589839C (en) * 2004-12-02 2016-04-05 Unilever N.V. Method for affinity purification
GB2446550B (en) 2005-11-28 2010-12-08 Proteomtech Inc Methods for production of recombinant alpha1-antitrypsin
WO2007092579A2 (en) * 2006-02-08 2007-08-16 Promega Corporation Compositions and methods for capturing and analyzing cross-linked biomolecules
US20100129889A1 (en) * 2006-09-05 2010-05-27 Innovative Purification Technologies Pty Ltd Affinity separation methods and systems
CN101054594A (zh) * 2007-03-30 2007-10-17 南开大学 双功能融合蛋白CBD－ProA载体及其制备的免疫磁性微球和应用
CN101910194B (zh) 2007-11-12 2017-03-08 科雷托公司 供纯化用的双亲和性多肽

Also Published As

Publication number Publication date
LT2220107T (lt) 2017-04-10
US20140081001A1 (en) 2014-03-20
EP2220107A2 (en) 2010-08-25
CN101910194B (zh) 2017-03-08
PL2220107T3 (pl) 2017-05-31
ES2613029T3 (es) 2017-05-22
CN101910194A (zh) 2010-12-08
AU2008322998B2 (en) 2013-10-03
US20110207916A1 (en) 2011-08-25
BRPI0820156A2 (pt) 2015-10-20
HRP20170069T1 (hr) 2017-03-24
SI2220107T1 (sl) 2017-02-28
WO2009062942A3 (en) 2010-02-18
DK2220107T3 (en) 2017-02-13
AU2008322998A1 (en) 2009-05-22
WO2009062942A2 (en) 2009-05-22
BRPI0820156B1 (pt) 2021-01-12
CA2705334C (en) 2018-04-17
EP2220107B1 (en) 2016-11-02
US9376463B2 (en) 2016-06-28
HUE032930T2 (hu) 2017-11-28
CA2705334A1 (en) 2009-05-22
PT2220107T (pt) 2017-02-08

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: CHRETO APS (DK)

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07D Technical examination (opinion) related to article 229 of industrial property law [chapter 7.4 patent gazette]
B07E Notification of approval relating to section 229 industrial property law [chapter 7.5 patent gazette]
B06U Preliminary requirement: requests with searches performed by other patent offices: procedure suspended [chapter 6.21 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 10 (DEZ) ANOS CONTADOS A PARTIR DE 12/01/2021, OBSERVADAS AS CONDICOES LEGAIS.

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 12/11/2008 OBSERVADAS AS CONDICOES LEGAIS. PATENTE CONCEDIDA CONFORME ADI 5.529/DF