BRPI0909212A2 - Composições e método para sequeciamento de ácido nucléico - Google Patents

Composições e método para sequeciamento de ácido nucléico

Info

Publication number
BRPI0909212A2
BRPI0909212A2 BRPI0909212-9A BRPI0909212A BRPI0909212A2 BR PI0909212 A2 BRPI0909212 A2 BR PI0909212A2 BR PI0909212 A BRPI0909212 A BR PI0909212A BR PI0909212 A2 BRPI0909212 A2 BR PI0909212A2
Authority
BR
Brazil
Prior art keywords
compositions
nucleic acid
acid sequencing
sequencing
nucleic
Prior art date
Application number
BRPI0909212-9A
Other languages
English (en)
Inventor
Kevin Travers
Geoff Otto
Stephen Turner
Cheryl Heiner
Congcong Ma
Original Assignee
Pacific Biosciences California
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=41114541&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0909212(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pacific Biosciences California filed Critical Pacific Biosciences California
Publication of BRPI0909212A2 publication Critical patent/BRPI0909212A2/pt

Links

Classifications

    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6853—Nucleic acid amplification reactions using modified primers or templates
    • C12Q1/6855—Ligating adaptors
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869—Methods for sequencing
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • G—PHYSICS
    • G16—INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR SPECIFIC APPLICATION FIELDS
    • G16B—BIOINFORMATICS, i.e. INFORMATION AND COMMUNICATION TECHNOLOGY [ICT] SPECIALLY ADAPTED FOR GENETIC OR PROTEIN-RELATED DATA PROCESSING IN COMPUTATIONAL MOLECULAR BIOLOGY
    • G16B30/00—ICT specially adapted for sequence analysis involving nucleotides or amino acids
    • G16B30/10—Sequence alignment; Homology search

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Organic Chemistry (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • General Health & Medical Sciences (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Genetics & Genomics (AREA)
  • General Engineering & Computer Science (AREA)
  • Biochemistry (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Theoretical Computer Science (AREA)
  • Bioinformatics & Computational Biology (AREA)
  • Evolutionary Biology (AREA)
  • Spectroscopy & Molecular Physics (AREA)
  • Medical Informatics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BRPI0909212-9A 2008-03-28 2009-03-27 Composições e método para sequeciamento de ácido nucléico BRPI0909212A2 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US7216008P 2008-03-28 2008-03-28
US9969608P 2008-09-24 2008-09-24
PCT/US2009/001926 WO2009120372A2 (en) 2008-03-28 2009-03-27 Compositions and methods for nucleic acid sequencing

Publications (1)

Publication Number Publication Date
BRPI0909212A2 true BRPI0909212A2 (pt) 2015-08-18

Family

ID=41114541

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0909212-9A BRPI0909212A2 (pt) 2008-03-28 2009-03-27 Composições e método para sequeciamento de ácido nucléico

Country Status (9)

Country Link
US (12) US8153375B2 (pt)
EP (6) EP3425060B1 (pt)
JP (1) JP2011515102A (pt)
CN (2) CN102084001B (pt)
AU (1) AU2009229157B2 (pt)
BR (1) BRPI0909212A2 (pt)
CA (2) CA2719747C (pt)
MX (1) MX2010010600A (pt)
WO (1) WO2009120372A2 (pt)

Families Citing this family (350)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US10337054B2 (en) 2004-02-02 2019-07-02 Quantum-Si Incorporated Enrichment of nucleic acid targets
GB0523282D0 (en) 2005-11-15 2005-12-21 Isis Innovation Methods using pores
WO2009020682A2 (en) 2007-05-08 2009-02-12 The Trustees Of Boston University Chemical functionalization of solid-state nanopores and nanopore arrays and applications thereof
CN104297506B (zh) 2007-10-02 2017-05-03 赛拉诺斯股份有限公司 模块化现场护理装置及其应用
GB2453377A (en) * 2007-10-05 2009-04-08 Isis Innovation Transmembrane protein pores and molecular adapters therefore.
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
US8420366B2 (en) * 2008-03-31 2013-04-16 Pacific Biosciences Of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
US8999676B2 (en) 2008-03-31 2015-04-07 Pacific Biosciences Of California, Inc. Recombinant polymerases for improved single molecule sequencing
EP2271751B1 (en) * 2008-03-31 2015-07-22 Pacific Biosciences of California, Inc. Generation of modified polymerases for improved accuracy in single molecule sequencing
CA2730068A1 (en) * 2008-07-07 2010-01-14 Oxford Nanopore Technologies Limited Base-detecting pore
US20110229877A1 (en) 2008-07-07 2011-09-22 Oxford Nanopore Technologies Limited Enzyme-pore constructs
CN102144031B (zh) * 2008-09-04 2013-07-17 中国农业科学院生物技术研究所 表达发卡rna的dna分子及其构建方法与应用
US8921046B2 (en) * 2008-09-19 2014-12-30 Pacific Biosciences Of California, Inc. Nucleic acid sequence analysis
WO2010048337A2 (en) * 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
EP2364368B1 (en) 2008-11-07 2014-01-15 Sequenta, Inc. Methods of monitoring conditions by sequence analysis
US9528160B2 (en) 2008-11-07 2016-12-27 Adaptive Biotechnolgies Corp. Rare clonotypes and uses thereof
US9394567B2 (en) 2008-11-07 2016-07-19 Adaptive Biotechnologies Corporation Detection and quantification of sample contamination in immune repertoire analysis
US9365901B2 (en) 2008-11-07 2016-06-14 Adaptive Biotechnologies Corp. Monitoring immunoglobulin heavy chain evolution in B-cell acute lymphoblastic leukemia
US8748103B2 (en) 2008-11-07 2014-06-10 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8628927B2 (en) 2008-11-07 2014-01-14 Sequenta, Inc. Monitoring health and disease status using clonotype profiles
US8486630B2 (en) * 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US9506119B2 (en) 2008-11-07 2016-11-29 Adaptive Biotechnologies Corp. Method of sequence determination using sequence tags
GB0820927D0 (en) 2008-11-14 2008-12-24 Isis Innovation Method
WO2010057185A1 (en) 2008-11-17 2010-05-20 Pacific Biosciences Of California, Inc. Phospholink nucleotides for sequencing applications
US8370079B2 (en) * 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US12527665B2 (en) 2008-12-11 2026-01-20 Pacific Biosciences Of California, Inc. Methods for identifying modified bases in nucleic acid templates
US9175338B2 (en) 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
ES2568509T3 (es) 2009-01-15 2016-04-29 Adaptive Biotechnologies Corporation Perfilado de la inmunidad adaptativa y métodos para la generación de anticuerpos monoclonales
CN102482330A (zh) 2009-01-30 2012-05-30 牛津纳米孔技术有限公司 酶突变体
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
GB0905140D0 (en) 2009-03-25 2009-05-06 Isis Innovation Method
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
CA2765949C (en) 2009-06-25 2016-03-29 Fred Hutchinson Cancer Research Center Method of measuring adaptive immunity
US9566335B1 (en) 2009-09-25 2017-02-14 The Governing Council Of The University Of Toronto Protein sequencing method and reagents
WO2011040996A1 (en) 2009-09-30 2011-04-07 Quantapore, Inc. Ultrafast sequencing of biological polymers using a labeled nanopore
GB2487341A (en) * 2009-11-02 2012-07-18 Nugen Technologies Inc Compositions and methods for targeted nucleic acid sequence selection and amplification
EP2933629B1 (en) 2010-02-19 2019-04-10 Pacific Biosciences Of California, Inc. System for measuring analytical reactions comprising a socket for an optode array chip
US20110224106A1 (en) 2010-03-10 2011-09-15 Ibis Biosciences, Inc. Production Of Single-Stranded Circular Nucleic Acid
US9074251B2 (en) 2011-02-10 2015-07-07 Illumina, Inc. Linking sequence reads using paired code tags
WO2012092260A1 (en) * 2010-12-27 2012-07-05 Ibis Biosciences, Inc. Compositions and methods for producing single-stranded circular dna
CN103477197B (zh) 2011-01-21 2016-08-17 提拉诺斯公司 样品使用最大化的系统和方法
JP6017458B2 (ja) * 2011-02-02 2016-11-02 ユニヴァーシティ・オブ・ワシントン・スルー・イッツ・センター・フォー・コマーシャリゼーション 大量並列連続性マッピング
BR112013020411B1 (pt) 2011-02-11 2021-09-08 Oxford Nanopore Technologies Limited Monômero de msp mutante, construto, polinucleotídeo, poro, kit e aparelho para caracterizar uma sequência de ácido nucleico alvo, e, método para caracterizar uma sequência de ácido nucleico alvo
WO2012121756A1 (en) * 2011-03-04 2012-09-13 Quantapore, Inc. Apparatus and methods for performing optical nanopore detection or sequencing
EP2689028B1 (en) 2011-03-23 2017-08-30 Pacific Biosciences Of California, Inc. Isolation of polymerase-nucleic acid complexes and loading onto substrates
US9260753B2 (en) 2011-03-24 2016-02-16 President And Fellows Of Harvard College Single cell nucleic acid detection and analysis
WO2012138921A1 (en) 2011-04-08 2012-10-11 Life Technologies Corporation Phase-protecting reagent flow orderings for use in sequencing-by-synthesis
EP3246416B1 (en) 2011-04-15 2024-06-05 The Johns Hopkins University Safe sequencing system
KR20140050067A (ko) 2011-07-25 2014-04-28 옥스포드 나노포어 테크놀로지즈 리미티드 막횡단 포어를 사용한 이중 가닥 폴리뉴클레오티드 서열분석을 위한 헤어핀 루프 방법
EP3225698B1 (en) * 2011-09-06 2019-07-31 Gen-Probe Incorporated Closed nucleic acid structures
WO2013036668A1 (en) * 2011-09-06 2013-03-14 Gen-Probe Incorporated Circularized templates for sequencing
US10385475B2 (en) 2011-09-12 2019-08-20 Adaptive Biotechnologies Corp. Random array sequencing of low-complexity libraries
US9664702B2 (en) 2011-09-25 2017-05-30 Theranos, Inc. Fluid handling apparatus and configurations
US8435738B2 (en) 2011-09-25 2013-05-07 Theranos, Inc. Systems and methods for multi-analysis
US9268915B2 (en) 2011-09-25 2016-02-23 Theranos, Inc. Systems and methods for diagnosis or treatment
US8475739B2 (en) 2011-09-25 2013-07-02 Theranos, Inc. Systems and methods for fluid handling
US9619627B2 (en) 2011-09-25 2017-04-11 Theranos, Inc. Systems and methods for collecting and transmitting assay results
US20140170735A1 (en) 2011-09-25 2014-06-19 Elizabeth A. Holmes Systems and methods for multi-analysis
US9632102B2 (en) 2011-09-25 2017-04-25 Theranos, Inc. Systems and methods for multi-purpose analysis
US8840838B2 (en) 2011-09-25 2014-09-23 Theranos, Inc. Centrifuge configurations
US9810704B2 (en) 2013-02-18 2017-11-07 Theranos, Inc. Systems and methods for multi-analysis
US9250229B2 (en) 2011-09-25 2016-02-02 Theranos, Inc. Systems and methods for multi-analysis
US10012664B2 (en) 2011-09-25 2018-07-03 Theranos Ip Company, Llc Systems and methods for fluid and component handling
US10152569B2 (en) 2011-09-26 2018-12-11 Gen-Probe Incorporated Algorithms for sequence determinations
WO2013056241A2 (en) 2011-10-14 2013-04-18 Pacific Biosciences Of California, Inc. Real-time redox sequencing
EP2769007B1 (en) 2011-10-19 2016-12-07 Nugen Technologies, Inc. Compositions and methods for directional nucleic acid amplification and sequencing
HK1201565A1 (en) 2011-10-21 2015-09-04 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
EP3388535B1 (en) 2011-12-09 2021-03-24 Adaptive Biotechnologies Corporation Diagnosis of lymphoid malignancies and minimal residual disease detection
JP6093498B2 (ja) * 2011-12-13 2017-03-08 株式会社日立ハイテクノロジーズ 核酸増幅方法
US9499865B2 (en) 2011-12-13 2016-11-22 Adaptive Biotechnologies Corp. Detection and measurement of tissue-infiltrating lymphocytes
US9404147B2 (en) 2011-12-19 2016-08-02 Gen-Probe Incorporated Closed nucleic acid structures
WO2013096819A2 (en) 2011-12-22 2013-06-27 Ibis Biosciences, Inc. Macromolecule positioning by electrical potential
US9803231B2 (en) 2011-12-29 2017-10-31 Ibis Biosciences, Inc. Macromolecule delivery to nanowells
WO2013109731A1 (en) * 2012-01-18 2013-07-25 Singular Bio Inc. Methods for mapping bar-coded molecules for structural variation detection and sequencing
CA2861457A1 (en) * 2012-01-20 2013-07-25 Genia Technologies, Inc. Nanopore based molecular detection and sequencing
CN105861487B (zh) 2012-01-26 2020-05-05 纽亘技术公司 用于靶向核酸序列富集和高效文库产生的组合物和方法
US9238836B2 (en) 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9528107B2 (en) 2012-01-31 2016-12-27 Pacific Biosciences Of California, Inc. Compositions and methods for selection of nucleic acids
EP3222627B1 (en) 2012-02-15 2019-08-07 Pacific Biosciences of California, Inc. Polymerase enzyme substrates with protein shield
US10077478B2 (en) 2012-03-05 2018-09-18 Adaptive Biotechnologies Corp. Determining paired immune receptor chains from frequency matched subunits
WO2013142389A1 (en) 2012-03-20 2013-09-26 University Of Washington Through Its Center For Commercialization Methods of lowering the error rate of massively parallel dna sequencing using duplex consensus sequencing
EP2831283A4 (en) 2012-03-30 2015-11-04 Pacific Biosciences California METHODS AND COMPOSITION FOR SEQUENCING MODIFIED NUCLEIC ACIDS
WO2013153359A1 (en) 2012-04-10 2013-10-17 Oxford Nanopore Technologies Limited Mutant lysenin pores
WO2013155223A1 (en) 2012-04-10 2013-10-17 The Regents Of The University Of California Compositions and methods for treating cancer
WO2013163207A1 (en) 2012-04-24 2013-10-31 Pacific Biosciences Of California, Inc. Identification of 5-methyl-c in nucleic acid templates
PT2831276T (pt) 2012-05-08 2016-07-26 Adaptive Biotechnologies Corp Composições e método para medição e calibração do viés de amplificação em reações pcr multiplex
AU2013267609C1 (en) 2012-05-31 2019-01-03 Board Of Regents, The University Of Texas System Method for accurate sequencing of DNA
AU2013205064B2 (en) 2012-06-04 2015-07-30 Gen-Probe Incorporated Compositions and Methods for Amplifying and Characterizing HCV Nucleic Acid
GB2559073A (en) 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
US9267168B2 (en) 2012-06-12 2016-02-23 Pacific Biosciences Of California, Inc. Methods and compositions for isolating template nucleic acids
CA2877094A1 (en) 2012-06-18 2013-12-27 Nugen Technologies, Inc. Compositions and methods for negative selection of non-desired nucleic acid sequences
US20150011396A1 (en) 2012-07-09 2015-01-08 Benjamin G. Schroeder Methods for creating directional bisulfite-converted nucleic acid libraries for next generation sequencing
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
EP2880182B1 (en) 2012-08-02 2017-07-26 Qiagen GmbH Recombinase mediated targeted dna enrichment for next generation sequencing
US11913065B2 (en) 2012-09-04 2024-02-27 Guardent Health, Inc. Systems and methods to detect rare mutations and copy number variation
US20160040229A1 (en) 2013-08-16 2016-02-11 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
US10876152B2 (en) 2012-09-04 2020-12-29 Guardant Health, Inc. Systems and methods to detect rare mutations and copy number variation
PL2893040T3 (pl) 2012-09-04 2019-05-31 Guardant Health Inc Sposoby wykrywania rzadkich mutacji i wariantu liczby kopii
WO2014044724A1 (en) 2012-09-18 2014-03-27 Qiagen Gmbh Method and kit for preparing a target rna depleted sample
EP2904111B1 (en) 2012-10-01 2017-12-06 Adaptive Biotechnologies Corporation Immunocompetence assessment by adaptive immune receptor diversity and clonality characterization
US9399766B2 (en) 2012-10-01 2016-07-26 Pacific Biosciences Of California, Inc. Recombinant polymerases for incorporation of protein shield nucleotide analogs
GB201217772D0 (en) * 2012-10-04 2012-11-14 Base4 Innovation Ltd Sequencing method
GB201217770D0 (en) 2012-10-04 2012-11-14 Base4 Innovation Ltd Biological probes and the use thereof
US10000802B2 (en) 2012-10-04 2018-06-19 Base4 Innovation Ltd Sequencing method
WO2015160439A2 (en) 2014-04-17 2015-10-22 Adaptive Biotechnologies Corporation Quantification of adaptive immune cell genomes in a complex mixture of cells
US9651539B2 (en) 2012-10-28 2017-05-16 Quantapore, Inc. Reducing background fluorescence in MEMS materials by low energy ion beam treatment
US9683230B2 (en) 2013-01-09 2017-06-20 Illumina Cambridge Limited Sample preparation on a solid support
US10745686B2 (en) 2013-02-08 2020-08-18 Qiagen Gmbh Method for separating DNA by size
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
US20140274733A1 (en) * 2013-03-12 2014-09-18 Life Technologies Corporation Methods and Systems for Local Sequence Alignment
EP2970951B1 (en) 2013-03-13 2019-02-20 Illumina, Inc. Methods for nucleic acid sequencing
EP2971130A4 (en) 2013-03-15 2016-10-05 Nugen Technologies Inc SEQUENTIAL SEQUENCING
US10732183B2 (en) * 2013-03-15 2020-08-04 The Trustees Of Columbia University In The City Of New York Method for detecting multiple predetermined compounds in a sample
US9290800B2 (en) 2013-03-15 2016-03-22 Pacific Biosciences Of California, Inc. Targeted rolling circle amplification
US9227978B2 (en) 2013-03-15 2016-01-05 Araxes Pharma Llc Covalent inhibitors of Kras G12C
US10450595B2 (en) 2013-03-15 2019-10-22 Theranos Ip Company, Llc Nucleic acid amplification
WO2014150851A1 (en) 2013-03-15 2014-09-25 Ibis Biosciences, Inc. Nucleotide analogs for sequencing
AU2014233145A1 (en) * 2013-03-15 2015-09-17 Theranos Ip Company, Llc Nucleic acid amplification
SG11201507261WA (en) 2013-03-15 2015-10-29 Theranos Inc Nucleic acid amplification
US9328382B2 (en) 2013-03-15 2016-05-03 Complete Genomics, Inc. Multiple tagging of individual long DNA fragments
WO2014145296A2 (en) 2013-03-15 2014-09-18 Theranos, Inc. Nuclei acid amplification
GB201313477D0 (en) 2013-07-29 2013-09-11 Univ Leuven Kath Nanopore biosensors for detection of proteins and nucleic acids
CN111187811B (zh) 2013-05-06 2024-03-08 加利福尼亚太平洋生物科学股份有限公司 实时电子测序
US9862997B2 (en) 2013-05-24 2018-01-09 Quantapore, Inc. Nanopore-based nucleic acid analysis with mixed FRET detection
US9708657B2 (en) 2013-07-01 2017-07-18 Adaptive Biotechnologies Corp. Method for generating clonotype profiles using sequence tags
US9879318B2 (en) 2013-09-06 2018-01-30 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
TWI659021B (zh) 2013-10-10 2019-05-11 亞瑞克西斯製藥公司 Kras g12c之抑制劑
US9416414B2 (en) 2013-10-24 2016-08-16 Pacific Biosciences Of California, Inc. Delaying real-time sequencing
JP6525473B2 (ja) 2013-11-13 2019-06-05 ニューゲン テクノロジーズ, インコーポレイテッド 複製物配列決定リードを同定するための組成物および方法
EP3709003A1 (en) 2013-11-17 2020-09-16 Quantum-Si Incorporated Optical system and assay chip for probing, detecting and analyzing molecules
DK3083994T3 (da) 2013-12-20 2021-09-13 Illumina Inc Bevarelse af genomisk konnektivitetsinformation i fragmenterede genomiske DNA-prøver
CN111534580B (zh) 2013-12-28 2024-06-04 夸登特健康公司 用于检测遗传变异的方法和系统
EP3097210B2 (en) 2014-01-22 2022-04-20 Oxford Nanopore Technologies Limited Method for attaching one or more polynucleotide binding proteins to a target polynucleotide
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
WO2015131107A1 (en) 2014-02-28 2015-09-03 Nugen Technologies, Inc. Reduced representation bisulfite sequencing with diversity adaptors
CA2941612A1 (en) 2014-03-05 2015-09-11 Adaptive Biotechnologies Corporation Methods using randomer-containing synthetic molecules
US10066265B2 (en) 2014-04-01 2018-09-04 Adaptive Biotechnologies Corp. Determining antigen-specific t-cells
JP7058502B2 (ja) * 2014-04-11 2022-04-22 レッドボールト バイオサイエンシズ エルピー ゲノム適用および治療適用のための、核酸分子のクローン複製および増幅のためのシステムおよび方法
EP2940136A1 (en) 2014-04-30 2015-11-04 QIAGEN GmbH Method for isolating poly(A) nucleic acids
US10443097B2 (en) 2014-05-02 2019-10-15 Oxford Nanopore Technologies Ltd. Method of improving the movement of a target polynucleotide with respect to a transmembrane pore
EP3177740B1 (en) 2014-08-06 2021-01-13 Nugen Technologies, Inc. Digital measurements from targeted sequencing
US10174363B2 (en) 2015-05-20 2019-01-08 Quantum-Si Incorporated Methods for nucleic acid sequencing
CN111710687B (zh) 2014-08-08 2023-11-14 宽腾矽公司 基于入射光子到达时间的识别、成像、测序法及存储介质
KR102738114B1 (ko) 2014-08-08 2024-12-06 퀀텀-에스아이 인코포레이티드 분자들을 프로빙, 검출 및 분석하기 위한 광학계 및 검정 칩
US9885657B2 (en) 2014-08-08 2018-02-06 Quantum-Si Incorporated Integrated device with external light source for probing detecting and analyzing molecules
US12655467B2 (en) 2014-08-08 2026-06-16 Quantum-Si Incorporated Methods for nucleic acid sequencing
EP3183367B1 (en) 2014-08-19 2019-06-26 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
US10435685B2 (en) 2014-08-19 2019-10-08 Pacific Biosciences Of California, Inc. Compositions and methods for enrichment of nucleic acids
CN119899249A (zh) 2014-09-01 2025-04-29 弗拉芒区生物技术研究所 突变csgg孔
WO2016037358A1 (zh) 2014-09-12 2016-03-17 深圳华大基因科技有限公司 分离的寡核苷酸及其在核酸测序中的用途
WO2016037361A1 (zh) * 2014-09-12 2016-03-17 深圳华大基因科技有限公司 试剂盒及其在核酸测序中的用途
JO3556B1 (ar) 2014-09-18 2020-07-05 Araxes Pharma Llc علاجات مدمجة لمعالجة السرطان
US9862701B2 (en) 2014-09-25 2018-01-09 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
US10011600B2 (en) 2014-09-25 2018-07-03 Araxes Pharma Llc Methods and compositions for inhibition of Ras
US10266885B2 (en) 2014-10-07 2019-04-23 Oxford Nanopore Technologies Ltd. Mutant pores
EP3204519B1 (en) 2014-10-10 2020-04-29 Quantapore Inc. Nanopore-based polymer analysis with mutually-quenching fluorescent labels
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
CN107002126B (zh) 2014-10-24 2021-05-25 昆塔波尔公司 使用纳米结构阵列的聚合物的高效光学分析
CA2966201A1 (en) 2014-10-29 2016-05-06 Adaptive Biotechnologies Corp. Highly-multiplexed simultaneous detection of nucleic acids encoding paired adaptive immune receptor heterodimers from many samples
US10000799B2 (en) 2014-11-04 2018-06-19 Boreal Genomics, Inc. Methods of sequencing with linked fragments
WO2016077313A1 (en) 2014-11-11 2016-05-19 Bgi Shenzhen Multi-pass sequencing
US10246701B2 (en) 2014-11-14 2019-04-02 Adaptive Biotechnologies Corp. Multiplexed digital quantitation of rearranged lymphoid receptors in a complex mixture
EP3498866B1 (en) 2014-11-25 2026-02-25 Adaptive Biotechnologies Corp. Characterization of adaptive immune response to vaccination or infection using immune repertoire sequencing
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US10429342B2 (en) 2014-12-18 2019-10-01 Edico Genome Corporation Chemically-sensitive field effect transistor
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
GB201502810D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Method
GB201502809D0 (en) 2015-02-19 2015-04-08 Oxford Nanopore Tech Ltd Mutant pore
WO2016138122A1 (en) 2015-02-24 2016-09-01 Adaptive Biotechnologies Corp. Methods for diagnosing infectious disease and determining hla status using immune repertoire sequencing
JP6560336B2 (ja) 2015-02-26 2019-08-14 株式会社日立ハイテクノロジーズ 核酸分子の構築方法
EP3274473B1 (en) 2015-03-24 2020-10-28 Pacific Biosciences of California, Inc. Methods and compositions for single molecule composition loading
EP3277294B1 (en) 2015-04-01 2024-05-15 Adaptive Biotechnologies Corp. Method of identifying human compatible t cell receptors specific for an antigenic target
AU2016245864C1 (en) 2015-04-10 2021-09-09 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
CN107735686B (zh) 2015-04-14 2021-06-11 鲁汶天主教大学 具有内部蛋白质衔接子的纳米孔
ES2856880T3 (es) 2015-04-15 2021-09-28 Araxes Pharma Llc Inhibidores tricíclicos condensados de KRAS y métodos de uso de los mismos
US10385387B2 (en) 2015-04-20 2019-08-20 Pacific Biosciences Of California, Inc. Methods for selectively amplifying and tagging nucleic acids
CA2983882C (en) 2015-05-07 2023-10-03 Scott E. Helgesen Multiprocessor pipeline architecture
WO2016185284A1 (en) 2015-05-20 2016-11-24 Boreal Genomics, Inc. Method for isolating target nucleic acid using heteroduplex binding proteins
CN107615283B (zh) 2015-05-26 2022-07-05 加利福尼亚太平洋生物科学股份有限公司 用于二倍体基因组组装和单倍型序列重建的方法、软件和系统
WO2016193490A1 (en) * 2015-06-05 2016-12-08 Qiagen Gmbh Method for separating dna by size
WO2016201142A1 (en) 2015-06-09 2016-12-15 Life Technologies Corporation Methods, systems, compositions, kits, apparatus and computer-readable media for molecular tagging
US10144724B2 (en) 2015-07-22 2018-12-04 Araxes Pharma Llc Substituted quinazoline compounds and methods of use thereof
EP3332033B1 (en) 2015-08-06 2021-04-21 Pacific Biosciences of California, Inc. Single-molecule nanofet sequencing systems and methods
WO2017058768A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
EP3356347A1 (en) 2015-09-28 2018-08-08 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
US10730867B2 (en) 2015-09-28 2020-08-04 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
WO2017058902A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
WO2017058728A1 (en) 2015-09-28 2017-04-06 Araxes Pharma Llc Inhibitors of kras g12c mutant proteins
US10975071B2 (en) 2015-09-28 2021-04-13 Araxes Pharma Llc Inhibitors of KRAS G12C mutant proteins
EP3356359B1 (en) 2015-09-28 2021-10-20 Araxes Pharma LLC Inhibitors of kras g12c mutant proteins
JP2018533939A (ja) 2015-10-19 2018-11-22 アラクセス ファーマ エルエルシー Rasの阻害剤をスクリーニングするための方法
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
KR102932353B1 (ko) 2015-11-16 2026-02-27 아락세스 파마 엘엘씨 치환된 헤테로사이클릭 그룹을 포함하는 2-치환된 퀴나졸린 화합물 및 이의 사용 방법
US10814299B2 (en) 2015-11-18 2020-10-27 Pacific Biosciences Of California, Inc. Loading nucleic acids onto substrates
US10731211B2 (en) 2015-11-18 2020-08-04 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
WO2017100441A1 (en) 2015-12-08 2017-06-15 Twinstrand Biosciences, Inc. Improved adapters, methods, and compositions for duplex sequencing
CN109072295B (zh) 2015-12-08 2022-05-31 鲁汶大学研究与发展中心 修饰的纳米孔,包含其的组合物及其应用
WO2017100546A1 (en) 2015-12-09 2017-06-15 Araxes Pharma Llc Methods for preparation of quinazoline derivatives
SG11201805119QA (en) 2015-12-17 2018-07-30 Guardant Health Inc Methods to determine tumor gene copy number by analysis of cell-free dna
TW202139919A (zh) 2016-02-17 2021-11-01 美商太斯萊特健康股份有限公司 用於生命期成像及偵測應用之感測器及裝置
KR102222191B1 (ko) 2016-03-02 2021-03-02 옥스포드 나노포어 테크놀로지즈 리미티드 돌연변이체 기공
US11384380B2 (en) 2016-03-16 2022-07-12 Yale University Compositions and methods for detecting N6-methyladenine in the mammalian genome
WO2017162754A1 (en) * 2016-03-22 2017-09-28 Vib Vzw Means and methods for amplifying nucleotide sequences
US10961573B2 (en) 2016-03-28 2021-03-30 Boreal Genomics, Inc. Linked duplex target capture
WO2017168331A1 (en) 2016-03-28 2017-10-05 Boreal Genomics, Inc. Linked duplex fragment sequencing
WO2017172979A1 (en) 2016-03-30 2017-10-05 Araxes Pharma Llc Substituted quinazoline compounds and methods of use
WO2017174572A1 (en) * 2016-04-04 2017-10-12 F. Hoffmann-La Roche Ag Nucleic acid sample preparation methods
CN118272512A (zh) 2016-04-06 2024-07-02 牛津纳米孔科技公开有限公司 突变体孔
US11384382B2 (en) 2016-04-14 2022-07-12 Guardant Health, Inc. Methods of attaching adapters to sample nucleic acids
WO2017181146A1 (en) 2016-04-14 2017-10-19 Guardant Health, Inc. Methods for early detection of cancer
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
WO2017209891A1 (en) * 2016-05-31 2017-12-07 Quantapore, Inc. Two-color nanopore sequencing
CN107488656B (zh) * 2016-06-13 2020-07-17 陆欣华 一种核酸等温自扩增方法
US10544457B2 (en) 2016-06-14 2020-01-28 Pacific Biosciences Of California, Inc. Methods and compositions for enriching compositions for polymerase enzyme complexes
US10370701B2 (en) 2016-06-17 2019-08-06 Pacific Biosciences Of California, Inc. Methods and compositions for generating asymmetrically-tagged nucleic acid fragments
US10590451B2 (en) 2016-07-01 2020-03-17 Personal Genomics, Inc. Methods of constructing a circular template and detecting DNA molecules
EP3482196B1 (en) 2016-07-05 2022-02-23 Quantapore, Inc. Optically based nanopore sequencing
US10646488B2 (en) 2016-07-13 2020-05-12 Araxes Pharma Llc Conjugates of cereblon binding compounds and G12C mutant KRAS, HRAS or NRAS protein modulating compounds and methods of use thereof
JP6889769B2 (ja) * 2016-07-18 2021-06-18 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 核酸配列決定の非対称な鋳型および非対称な方法
CN109312391B (zh) * 2016-07-18 2022-06-03 豪夫迈·罗氏有限公司 生成用于单分子测序的单链环状dna文库的方法
US10711300B2 (en) 2016-07-22 2020-07-14 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
CN116732145A (zh) * 2016-08-16 2023-09-12 塔驰莱特Ip有限公司 闭合线性dna的生产
US10428325B1 (en) 2016-09-21 2019-10-01 Adaptive Biotechnologies Corporation Identification of antigen-specific B cell receptors
JP2019529484A (ja) 2016-09-29 2019-10-17 アラクセス ファーマ エルエルシー Kras g12c変異体タンパク質の阻害剤
EP3792922A1 (en) 2016-09-30 2021-03-17 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US9850523B1 (en) 2016-09-30 2017-12-26 Guardant Health, Inc. Methods for multi-resolution analysis of cell-free nucleic acids
US10377743B2 (en) 2016-10-07 2019-08-13 Araxes Pharma Llc Inhibitors of RAS and methods of use thereof
CN110312825A (zh) 2016-10-24 2019-10-08 吉内恩福赛克公司 隐藏核酸内存在的信息
JP7048609B2 (ja) 2016-12-09 2022-04-05 ボリアル ジェノミクス, インコーポレイテッド 連結型ライゲーション
CN110291084A (zh) 2016-12-15 2019-09-27 加利福尼亚大学董事会 用于治疗癌症的组合物和方法
WO2018118997A2 (en) 2016-12-19 2018-06-28 Quantum-Si Incorporated Polymerizing enzymes for sequencing reactions
JP6847499B2 (ja) 2016-12-20 2021-03-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 環状コンセンサスシークエンシングのための一本鎖環状dnaライブラリー
CN110168732B (zh) 2016-12-22 2024-03-08 宽腾矽公司 具有直接合并像素的整合式光电侦测器
EP3573971A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC 1-(3-(6-(3-hydroxynaphthalen-1-yl)benzofuran-2-yl)azetidin-1yl)prop-2-en-1-one derivatives and similar compounds as kras g12c modulators for treating cancer
US11136308B2 (en) 2017-01-26 2021-10-05 Araxes Pharma Llc Substituted quinazoline and quinazolinone compounds and methods of use thereof
EP3573954A1 (en) 2017-01-26 2019-12-04 Araxes Pharma LLC Fused bicyclic benzoheteroaromatic compounds and methods of use thereof
JP7327802B2 (ja) 2017-01-26 2023-08-16 アラクセス ファーマ エルエルシー 縮合ヘテロ-ヘテロ二環式化合物およびその使用方法
WO2018140599A1 (en) 2017-01-26 2018-08-02 Araxes Pharma Llc Benzothiophene and benzothiazole compounds and methods of use thereof
US10894979B2 (en) 2017-01-27 2021-01-19 Integrated Dna Technologies, Inc. Construction of next generation sequencing (NGS) libraries using competitive strand displacement
JP6982087B2 (ja) * 2017-01-27 2021-12-17 インテグレイテッド ディエヌエイ テクノロジイズ, インコーポレイテッドIntegrated Dna Technologies, Incorporated 競合的鎖置換を利用する次世代シーケンシング(ngs)ライブラリーの構築
US12492430B2 (en) 2017-04-11 2025-12-09 Tecan Genomics, Inc. Library quantitation and qualification
CN106906211B (zh) * 2017-04-13 2020-11-20 苏州普瑞迈德医学检验所有限公司 一种分子接头及其应用
GB201707122D0 (en) 2017-05-04 2017-06-21 Oxford Nanopore Tech Ltd Pore
JP7356909B2 (ja) 2017-05-05 2023-10-05 クアンタム-エスアイ インコーポレイテッド 生物学的反応における表面反応性と防汚特性が改変された基板
CN110869358A (zh) 2017-05-25 2020-03-06 亚瑞克西斯制药公司 Kras的共价抑制剂
TW201906832A (zh) 2017-05-25 2019-02-16 美商亞瑞克西斯製藥公司 用於癌症治療之化合物及其使用方法
WO2018218069A1 (en) 2017-05-25 2018-11-29 Araxes Pharma Llc Quinazoline derivatives as modulators of mutant kras, hras or nras
CN107423554B (zh) * 2017-06-09 2020-02-18 东南大学 一种用可逆单分子反应实现马尔科夫链的设计方法
EP3645552B1 (en) 2017-06-30 2023-06-28 Vib Vzw Novel protein pores
CN111094586A (zh) 2017-07-18 2020-05-01 加利福尼亚太平洋生物科学股份有限公司 用于分离不对称核酸复合物的方法和组合物
CA3069983A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated Hand-held, massively-parallel, bio-optoelectronic instrument
WO2019023257A1 (en) 2017-07-24 2019-01-31 Quantum-Si Incorporated HIGH INTENSITY MARKED REAGENT COMPOSITIONS AND SEQUENCING METHODS
JP2020532976A (ja) 2017-09-14 2020-11-19 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft 環状一本鎖dnaライブラリーを生成するための新規な方法
EP3682027B1 (en) 2017-09-15 2025-02-26 F. Hoffmann-La Roche AG Hybridization-extension-ligation strategy for generating circular single-stranded dna libraries
GB201715684D0 (en) 2017-09-28 2017-11-15 Univ Gent Means and methods for single molecule peptide sequencing
WO2019068797A1 (en) 2017-10-06 2019-04-11 F. Hoffmann-La Roche Ag CIRCULARIZATION METHODS FOR THE PREPARATION OF SAMPLE SAMPLES OF UNIQUE MOLECULES
US10501739B2 (en) 2017-10-18 2019-12-10 Mission Bio, Inc. Method, systems and apparatus for single cell analysis
US11099202B2 (en) 2017-10-20 2021-08-24 Tecan Genomics, Inc. Reagent delivery system
US11162138B2 (en) 2017-10-30 2021-11-02 Pacific Biosciences Of California, Inc. Multi-amplitude modular labeled compounds
WO2019086531A1 (en) 2017-11-03 2019-05-09 F. Hoffmann-La Roche Ag Linear consensus sequencing
CA3081027A1 (en) 2017-11-08 2019-05-16 Twinstrand Biosciences, Inc. Reagents and adapters for nucleic acid sequencing and methods for making such reagents and adapters
US11254980B1 (en) 2017-11-29 2022-02-22 Adaptive Biotechnologies Corporation Methods of profiling targeted polynucleotides while mitigating sequencing depth requirements
CN111936634B (zh) * 2017-12-11 2025-05-09 Umc乌德勒支控股有限公司 用于制备用于测序的核酸分子的方法
WO2019126734A1 (en) 2017-12-22 2019-06-27 Pacific Biosciences Of California, Inc. Modified biotin-binding proteins for immobilization
CN111801427B (zh) * 2018-02-05 2023-12-05 豪夫迈·罗氏有限公司 用于单分子的单链环状dna模板的产生
CN108359723B (zh) * 2018-02-23 2021-06-08 奥明(杭州)基因科技有限公司 一种降低深度测序错误的方法
WO2019166565A1 (en) 2018-03-02 2019-09-06 F. Hoffmann-La Roche Ag Generation of double-stranded dna templates for single molecule sequencing
WO2019183640A1 (en) 2018-03-23 2019-09-26 Board Of Regents, The University Of Texas System Efficient sequencing of dsdna with extremely low level of errors
WO2019204720A1 (en) * 2018-04-20 2019-10-24 The Regents Of The University Of California Nucleic acid sequencing methods and computer-readable media for practicing same
WO2019209946A1 (en) * 2018-04-25 2019-10-31 Qiagen Sciences Llc Sequential paired-end sequencing
ES2947437T3 (es) 2018-05-08 2023-08-09 Mgi Tech Co Ltd Creación de Códigos de barra compartidos en el ADN, en un solo tubo con perlas, para la secuenciación, haplotipado y ensamblaje preciso y rentable
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
CN121472373A (zh) 2018-05-22 2026-02-06 安序源有限公司 用于核酸测序的方法、系统和组合物
JP2021525095A (ja) 2018-05-28 2021-09-24 エフ.ホフマン−ラ ロシュ アーゲーF. Hoffmann−La Roche Aktiengesellschaft Dna−ポア−ポリメラーゼ複合体の酵素的濃縮
MX2020013788A (es) 2018-06-22 2021-03-02 Quantum Si Inc Fotodetector integrado con deposito de almacenamiento de carga con tiempo de deteccion variado.
EP3814531A4 (en) 2018-06-29 2022-04-06 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
WO2020002621A2 (en) 2018-06-29 2020-01-02 F. Hoffmann-La Roche Ag Detection of microsatellite instability
AU2019300172A1 (en) 2018-07-12 2021-01-28 Twinstrand Biosciences, Inc. Methods and reagents for characterizing genomic editing, clonal expansion, and associated applications
MX2021000470A (es) 2018-07-13 2021-06-23 Quantum Si Inc Etiquetas biconjugables y métodos de uso.
BR112021001709A2 (pt) 2018-08-01 2021-05-04 Araxes Pharma Llc compostos espiro heterocíclicos e métodos de uso dos mesmos para o tratamento de câncer
US12098419B2 (en) 2018-08-23 2024-09-24 Ncan Genomics, Inc. Linked target capture and ligation
WO2020043803A1 (en) 2018-08-28 2020-03-05 Sophia Genetics S.A. Methods for asymmetric dna library generation and optionally integrated duplex sequencing
GB2613480B (en) * 2018-11-15 2023-11-22 Element Biosciences Inc Methods for generating circular nucleic acid molecules
TWI863941B (zh) 2018-11-15 2024-12-01 美商寬騰矽公司 用於蛋白質定序之方法及組合物
EP3887385B1 (en) 2018-11-30 2024-03-20 Geneinfosec, Inc. A method for generating random oligonucleotides and determining their sequence
EP3674702A1 (en) 2018-12-27 2020-07-01 Imec VZW Method for sequencing a polynucleotide using a biofet
EP4538389A3 (en) 2019-01-03 2025-07-30 Ncan Genomics, Inc. Linked target capture
CA3125458A1 (en) * 2019-01-04 2020-07-09 William Marsh Rice University Quantitative amplicon sequencing for multiplexed copy number variation detection and allele ratio quantitation
CN113614097A (zh) 2019-01-23 2021-11-05 宽腾矽公司 高强度标记的反应物组合物和测序方法
EP3918088B1 (en) * 2019-01-29 2024-03-13 MGI Tech Co., Ltd. High coverage stlfr
EP4567131A3 (en) 2019-01-31 2025-09-03 Guardant Health, Inc. Compositions and methods for isolating cell-free dna
US20220298505A1 (en) * 2019-06-26 2022-09-22 Berry Genomics Co., Ltd Method for constructing pacbio sequencing library
KR20220029708A (ko) 2019-06-28 2022-03-08 퀀텀-에스아이 인코포레이티드 서열분석 반응을 위한 중합 효소
WO2020260618A1 (en) 2019-06-28 2020-12-30 Qiagen Gmbh Method for separating nucleic acid molecules by size
EP4017977A1 (en) 2019-08-19 2022-06-29 F. Hoffmann-La Roche AG Single tube preparation of dna and rna for sequencing
EP4031679A1 (en) 2019-09-20 2022-07-27 F. Hoffmann-La Roche AG Immune repertoire profiling by primer extension target enrichment
CN114829669B (zh) 2019-10-11 2024-10-01 宽腾矽公司 气相表面改性
EP4051903A1 (en) 2019-10-29 2022-09-07 Quantum-Si Incorporated Peristaltic pumping of fluids and associated methods, systems, and devices
WO2021142769A1 (zh) * 2020-01-17 2021-07-22 深圳华大智造科技有限公司 同步进行dna正义链和反义链测序的方法
EP3851542A1 (en) 2020-01-20 2021-07-21 Tecan Genomics, Inc. Depletion of abundant uninformative sequences
EP4090667A1 (en) 2020-01-21 2022-11-23 Quantum-si Incorporated Compounds and methods for selective c-terminal labeling
CN113275053A (zh) 2020-02-03 2021-08-20 帝肯基因组学公司 试剂存储系统
EP4114966B1 (en) 2020-03-03 2025-02-26 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing double stranded nucleic acids
WO2021178893A2 (en) * 2020-03-06 2021-09-10 Singular Genomics Systems, Inc. Linked paired strand sequencing
JP7490071B2 (ja) 2020-03-11 2024-05-24 エフ. ホフマン-ラ ロシュ アーゲー シーケンシングのための新規核酸鋳型構造
AU2021276522A1 (en) 2020-05-20 2023-01-05 Quantum-Si Incorporated Methods and compositions for protein sequencing
CN116075596A (zh) 2020-08-07 2023-05-05 牛津纳米孔科技公开有限公司 鉴定核酸条形码的方法
EP4530358A3 (en) * 2020-09-15 2025-06-18 10x Genomics, Inc. Methods of releasing an extended capture probe from a substrate and uses of the same
US12378596B2 (en) 2020-12-03 2025-08-05 Roche Sequencing Solutions, Inc. Whole transcriptome analysis in single cells
CA3183716A1 (en) * 2020-12-10 2022-06-16 Lewis Kraft Image sensor structures and related methods
CN112680320A (zh) * 2021-01-21 2021-04-20 季华实验室 零模波导孔结构及其制备方法、以及其使用方法
WO2022171606A2 (en) 2021-02-09 2022-08-18 F. Hoffmann-La Roche Ag Methods for base-level detection of methylation in nucleic acids
CN115029424B (zh) * 2021-03-03 2025-05-16 郑州思昆生物工程有限公司 一种固定化模板多核苷酸配对末端测序方法
CN117062902A (zh) 2021-03-03 2023-11-14 豪夫迈·罗氏有限公司 用于从生物学样品中电泳提取核酸的装置和方法
WO2022248237A1 (en) 2021-05-24 2022-12-01 F. Hoffmann-La Roche Ag Enhancer oligonucleotides for nucleic acid hybridization
EP4367264A1 (en) * 2021-07-08 2024-05-15 Pacific Biosciences Of California, Inc. Paired-end sequencing methods and compositions
EP4396339A4 (en) * 2021-09-03 2025-08-06 Singular Genomics Systems Inc METHODS FOR DIFFERENTIATING MODIFIED NUCLEOBASES
WO2023049682A1 (en) 2021-09-22 2023-03-30 Illumina, Inc. Sequencing polynucleotides using nanopores
WO2023055752A2 (en) 2021-09-28 2023-04-06 Axbio Inc. Methods for processing a nucleic acid sample and compositions thereof
WO2023081485A1 (en) 2021-11-08 2023-05-11 Pacific Biosciences Of California, Inc. Stepwise sequencing of a polynucleotide with a homogenous reaction mixture
EP4540408A1 (en) 2022-06-14 2025-04-23 F. Hoffmann-La Roche AG Detection of epigenetic cytosine modification
KR20250027734A (ko) * 2022-06-18 2025-02-27 나노가미 게엠베하 단일 세포 시퀀싱 및 오류율 감소를 위한 방법
WO2024015962A1 (en) 2022-07-15 2024-01-18 Pacific Biosciences Of California, Inc. Blocked asymmetric hairpin adaptors
US20240271202A1 (en) * 2023-01-31 2024-08-15 New York University Ultra high-fidelity single molecule sequencing
JP2026524816A (ja) 2023-06-14 2026-07-24 エフ. ホフマン-ラ ロシュ アーゲー 加速された5-ホルミルシトシンのマーキングおよび核酸メチル化シーケンシングにおける使用
EP4750913A1 (en) 2023-07-26 2026-06-03 Caribou Biosciences, Inc. In vitro validation methods for cd19-targeting cell therapies
CN121443756A (zh) 2023-08-04 2026-01-30 加利福尼亚太平洋生物科学股份有限公司 5-甲基胞嘧啶的检测
EP4791894A1 (en) 2023-10-13 2026-08-19 Roche Sequencing Solutions, Inc. Amplification-free target enrichment workflow for direct detection of nucleic acid modifications
JPWO2025134188A1 (pt) * 2023-12-18 2025-06-26
WO2025193609A1 (en) 2024-03-11 2025-09-18 Caribou Biosciences, Inc. Methods and compositions for cost-effective assessment of nucleic acid transcripts and isoforms in cells
WO2025231432A1 (en) 2024-05-03 2025-11-06 Caribou Biosciences, Inc. In vivo gene editing with crispr systems
US20250354208A1 (en) 2024-05-14 2025-11-20 Robert Bosch Gmbh Method for sequencing a dna-strand
GB202407055D0 (en) 2024-05-17 2024-07-03 Oxford Nanopore Tech Plc Enhanced polynucleotide sequencing
WO2025244989A1 (en) 2024-05-21 2025-11-27 Pacific Biosciences Of California, Inc. Selectively functionalized nanoscale wells
FI20245658A1 (en) * 2024-05-23 2025-11-24 Genomill Health Oy Method for amplifying and storing circular nucleic acid molecules
FI20245660A1 (en) * 2024-05-23 2025-11-24 Genomill Health Oy Circular nucleic acid molecule and process for its production
WO2025251175A1 (zh) * 2024-06-03 2025-12-11 深圳华大智造科技股份有限公司 串联多拷贝核酸分子构建方法及其应用
WO2026006622A2 (en) * 2024-06-28 2026-01-02 Illumina, Inc. Sequencing full-complement polynucleotides using nanopores
WO2026019720A1 (en) 2024-07-15 2026-01-22 Pacific Biosciences Of California, Inc. Improved loading of molecules and complexes to reaction sites
WO2026050948A1 (zh) * 2024-09-04 2026-03-12 深圳华大生命科学研究院 核酸文库的构建方法及应用
GB202413618D0 (en) 2024-09-16 2024-10-30 Oxford Nanopore Tech Plc peptide characterisation
WO2026162912A1 (en) 2025-01-31 2026-08-06 Oxford Nanopore Technologies Plc Methods and systems for polymer measurements

Family Cites Families (253)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2433109A (en) 1937-03-16 1947-12-23 Fouron Pierre Chill mould for casting cylinder heads of air-cooled engines
US3873209A (en) 1973-12-10 1975-03-25 Bell Telephone Labor Inc Measurement of thin films by optical waveguiding technique
US4994373A (en) 1983-01-27 1991-02-19 Enzo Biochem, Inc. Method and structures employing chemically-labelled polynucleotide probes
US5200313A (en) 1983-08-05 1993-04-06 Miles Inc. Nucleic acid hybridization assay employing detectable anti-hybrid antibodies
US5171534A (en) 1984-01-16 1992-12-15 California Institute Of Technology Automated DNA sequencing technique
CA1338457C (en) 1986-08-22 1996-07-16 Henry A. Erlich Purified thermostable enzyme
US5270201A (en) 1988-03-24 1993-12-14 The General Hospital Corporation Artificial chromosome vector
US4962020A (en) 1988-07-12 1990-10-09 President And Fellows Of Harvard College DNA sequencing
US5198543A (en) 1989-03-24 1993-03-30 Consejo Superior Investigaciones Cientificas PHI29 DNA polymerase
US5001050A (en) 1989-03-24 1991-03-19 Consejo Superior Investigaciones Cientificas PHφ29 DNA polymerase
US5622868A (en) 1989-04-27 1997-04-22 Microbiological Research Authority Camr (Centre For Applied Microbiology & Research) Analytical apparatus utilizing a colorimetric or other optically detectable effect
GB8910880D0 (en) 1989-05-11 1989-06-28 Amersham Int Plc Sequencing method
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
US5302509A (en) 1989-08-14 1994-04-12 Beckman Instruments, Inc. Method for sequencing polynucleotides
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
IL97222A (en) 1990-02-16 1995-08-31 Orion Yhtymae Oy Method and reagent for determining specific nucleotide variations
US5401847A (en) 1990-03-14 1995-03-28 Regents Of The University Of California DNA complexes with dyes designed for energy transfer as fluorescent markers
EP0455067B1 (de) 1990-05-03 2003-02-26 F. Hoffmann-La Roche Ag Mikrooptischer Sensor
DE69132905T2 (de) 1990-12-06 2002-08-01 Affymetrix, Inc. (N.D.Ges.D.Staates Delaware) Detektion von Nukleinsäuresequenzen
US5455166A (en) 1991-01-31 1995-10-03 Becton, Dickinson And Company Strand displacement amplification
US5162654A (en) * 1991-02-01 1992-11-10 Wisconsin Alumni Research Foundation Detection apparatus for electrophoretic gels
US6004744A (en) 1991-03-05 1999-12-21 Molecular Tool, Inc. Method for determining nucleotide identity through extension of immobilized primer
US5518900A (en) 1993-01-15 1996-05-21 Molecular Tool, Inc. Method for generating single-stranded DNA molecules
FR2675803B1 (fr) 1991-04-25 1996-09-06 Genset Sa Oligonucleotides fermes, antisens et sens et leurs applications.
US5365455A (en) * 1991-09-20 1994-11-15 Vanderbilt University Method and apparatus for automatic nucleic acid sequence determination
US5405747A (en) 1991-09-25 1995-04-11 The Regents Of The University Of California Office Of Technology Transfer Method for rapid base sequencing in DNA and RNA with two base labeling
US6048690A (en) 1991-11-07 2000-04-11 Nanogen, Inc. Methods for electronic fluorescent perturbation for analysis and electronic perturbation catalysis for synthesis
US5243618A (en) 1991-11-22 1993-09-07 Hughes Aircraft Company Cavity resonator incorporating waveguide filter
AU3729393A (en) 1992-02-20 1993-09-13 State Of Oregon Acting By And Through The Oregon State Board Of Higher Education On Behalf Of Oregon State University, The Boomerand DNA amplification
US5308751A (en) 1992-03-23 1994-05-03 General Atomics Method for sequencing double-stranded DNA
US5470705A (en) 1992-04-03 1995-11-28 Applied Biosystems, Inc. Probe composition containing a binding domain and polymer chain and methods of use
US5350686A (en) 1992-04-16 1994-09-27 Pioneer Hi-Bred International, Inc. Microwave acceleration of enzyme-catalyzed modification of macromolecules
GB9208733D0 (en) 1992-04-22 1992-06-10 Medical Res Council Dna sequencing method
US5403708A (en) 1992-07-06 1995-04-04 Brennan; Thomas M. Methods and compositions for determining the sequence of nucleic acids
US6261808B1 (en) 1992-08-04 2001-07-17 Replicon, Inc. Amplification of nucleic acid molecules via circular replicons
US5503980A (en) 1992-11-06 1996-04-02 Trustees Of Boston University Positional sequencing by hybridization
ATE267877T1 (de) 1993-01-07 2004-06-15 Sequenom Inc Dns - sequenzierung durch massenspektronomie
US5465151A (en) 1993-01-21 1995-11-07 State Of Oregon Acting By And Through The State Board Of Higher Education On Behalf Of The University Of Oregon Sensors employing interference of electromagnetic waves passing through waveguides having functionalized surfaces
FR2703693B1 (fr) 1993-04-06 1995-07-13 Pasteur Institut Procédé rapide de détermination d'une séquence d'ADN et application au séquençage et au diagnostic.
US5714320A (en) 1993-04-15 1998-02-03 University Of Rochester Rolling circle synthesis of oligonucleotides and amplification of select randomized circular oligonucleotides
US5677196A (en) 1993-05-18 1997-10-14 University Of Utah Research Foundation Apparatus and methods for multi-analyte homogeneous fluoro-immunoassays
WO1995006138A1 (en) 1993-08-25 1995-03-02 The Regents Of The University Of California Microscopic method for detecting micromotions
US5470710A (en) 1993-10-22 1995-11-28 University Of Utah Automated hybridization/imaging device for fluorescent multiplex DNA sequencing
US5654419A (en) 1994-02-01 1997-08-05 The Regents Of The University Of California Fluorescent labels and their use in separations
US5629468A (en) * 1994-05-26 1997-05-13 Universtiy Of Tennessee Research Corporation Root stimulating bacteria
US5695934A (en) 1994-10-13 1997-12-09 Lynx Therapeutics, Inc. Massively parallel sequencing of sorted polynucleotides
FR2729392B1 (fr) * 1995-01-16 1997-03-28 Pasteur Institut Oligonucleotides pour la detection de salmonella
US5601982A (en) 1995-02-07 1997-02-11 Sargent; Jeannine P. Method and apparatus for determining the sequence of polynucleotides
US6103465A (en) * 1995-02-14 2000-08-15 The Perkin-Elmer Corporation Methods and reagents for typing HLA class I genes
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
US6362002B1 (en) 1995-03-17 2002-03-26 President And Fellows Of Harvard College Characterization of individual polymer molecules based on monomer-interface interactions
US5674683A (en) 1995-03-21 1997-10-07 Research Corporation Technologies, Inc. Stem-loop and circular oligonucleotides and method of using
US5961923A (en) 1995-04-25 1999-10-05 Irori Matrices with memories and uses thereof
US5648245A (en) 1995-05-09 1997-07-15 Carnegie Institution Of Washington Method for constructing an oligonucleotide concatamer library by rolling circle replication
US5677769A (en) 1995-05-30 1997-10-14 Imra America Optical sensor utilizing rare-earth-doped integrated-optic lasers
EP0745686A1 (en) 1995-06-01 1996-12-04 Roche Diagnostics GmbH The use of DNA polymerase 3'-intrinsic editing activity
US5856174A (en) 1995-06-29 1999-01-05 Affymetrix, Inc. Integrated nucleic acid diagnostic device
WO1997004131A1 (en) 1995-07-21 1997-02-06 Forsyth Dental Infirmary For Children Single primer amplification of polynucleotide hairpins
US5661028A (en) 1995-09-29 1997-08-26 Lockheed Martin Energy Systems, Inc. Large scale DNA microsequencing device
DK0862656T3 (da) * 1995-11-21 2001-04-09 Univ Yale Unimolekylær segmentamplifikation og -detektering
US5854033A (en) 1995-11-21 1998-12-29 Yale University Rolling circle replication reporter systems
US6027890A (en) 1996-01-23 2000-02-22 Rapigene, Inc. Methods and compositions for enhancing sensitivity in the analysis of biological-based assays
US6611634B2 (en) 1996-03-19 2003-08-26 University Of Utah Research Foundation Lens and associatable flow cell
EP0902885A4 (en) 1996-05-16 2006-09-27 Affymetrix Inc SYSTEMS AND METHODS FOR DETECTION OF BRANDED PRODUCTS
US5846727A (en) 1996-06-06 1998-12-08 Board Of Supervisors Of Louisiana State University And Agricultural & Mechanical College Microsystem for rapid DNA sequencing
GB9620209D0 (en) 1996-09-27 1996-11-13 Cemu Bioteknik Ab Method of sequencing DNA
KR20000048736A (ko) 1996-09-30 2000-07-25 아벤티스 레제아르히 운트 테히놀로기스 게엠베하 운트 콤파니 카게 수중에 용해되어 있거나 분산되어 있는 화학 물질을 검출하기 위한 광학 센서
US5858671A (en) 1996-11-01 1999-01-12 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
US6258533B1 (en) 1996-11-01 2001-07-10 The University Of Iowa Research Foundation Iterative and regenerative DNA sequencing method
WO1998033939A1 (en) 1997-01-31 1998-08-06 Hitachi, Ltd. Method for determining nucleic acid base sequence and apparatus therefor
US6403311B1 (en) 1997-02-12 2002-06-11 Us Genomics Methods of analyzing polymers using ordered label strategies
IL156002A0 (en) 1997-02-12 2003-12-23 Eugene Y Chan Methods and products for analyzing polymers
US5958738A (en) * 1997-03-24 1999-09-28 Roche Diagnostics Corporation Procedure for subtractive hybridization and difference analysis
JP2001517948A (ja) 1997-04-01 2001-10-09 グラクソ、グループ、リミテッド 核酸配列決定法
US5948616A (en) * 1997-05-14 1999-09-07 Musc Foundation For Research Development Methods and compositions of correlating tissue kallikrein gene promoter polymorphisms with essential hypertension
WO1999005315A2 (en) 1997-07-28 1999-02-04 Medical Biosystems Ltd. Nucleic acid sequence analysis
US6087099A (en) * 1997-09-08 2000-07-11 Myriad Genetics, Inc. Method for sequencing both strands of a double stranded DNA in a single sequencing reaction
US7348181B2 (en) 1997-10-06 2008-03-25 Trustees Of Tufts College Self-encoding sensor with microspheres
US6124120A (en) 1997-10-08 2000-09-26 Yale University Multiple displacement amplification
JP2001519538A (ja) 1997-10-10 2001-10-23 プレジデント・アンド・フェローズ・オブ・ハーバード・カレッジ 核酸アレイのレプリカ増幅
US6485944B1 (en) 1997-10-10 2002-11-26 President And Fellows Of Harvard College Replica amplification of nucleic acid arrays
JP3862845B2 (ja) 1998-02-05 2006-12-27 セイコーインスツル株式会社 近接場用光プローブ
US6049641A (en) 1998-02-24 2000-04-11 Gemfire Corporation Connection system for optical redundancy
WO1999053102A1 (en) 1998-04-16 1999-10-21 Packard Bioscience Company Analysis of polynucleotide sequence
GB9810350D0 (en) 1998-05-14 1998-07-15 Ciba Geigy Ag Organic compounds
US6326725B1 (en) 1998-05-26 2001-12-04 Micron Technology, Inc. Focusing electrode for field emission displays and method
DE19826758C1 (de) 1998-06-15 1999-10-21 Soft Gene Gmbh Darstellung von linearen kovalent geschlossenen DNA-Molekülen als Expressionskonstrukte
AU4355899A (en) 1998-06-26 2000-01-17 Visible Genetics Inc. Method for sequencing nucleic acids with reduced errors
GB9815702D0 (en) 1998-07-21 1998-09-16 Cambridge Imaging Ltd Improved imaging system for fluorescence assays
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
AU770831B2 (en) 1998-07-30 2004-03-04 Solexa Ltd. Arrayed biomolecules and their use in sequencing
GB0002310D0 (en) 2000-02-01 2000-03-22 Solexa Ltd Polynucleotide sequencing
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
EP1105535A1 (en) 1998-08-13 2001-06-13 U.S. Genomics Optically characterizing polymers
US6263286B1 (en) 1998-08-13 2001-07-17 U.S. Genomics, Inc. Methods of analyzing polymers using a spatial network of fluorophores and fluorescence resonance energy transfer
US6280939B1 (en) 1998-09-01 2001-08-28 Veeco Instruments, Inc. Method and apparatus for DNA sequencing using a local sensitive force detector
US6203985B1 (en) 1998-09-08 2001-03-20 Motorola, Inc. Bio-molecule analyzer with photosensitive material and fabrication
WO2000015779A2 (en) 1998-09-15 2000-03-23 Yale University Molecular cloning using rolling circle amplification
US6235502B1 (en) * 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
DE19844931C1 (de) 1998-09-30 2000-06-15 Stefan Seeger Verfahren zur DNS- oder RNS-Sequenzierung
US6221592B1 (en) 1998-10-20 2001-04-24 Wisconsin Alumi Research Foundation Computer-based methods and systems for sequencing of individual nucleic acid molecules
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
NO986133D0 (no) 1998-12-23 1998-12-23 Preben Lexow FremgangsmÕte for DNA-sekvensering
GB9828785D0 (en) 1998-12-30 1999-02-17 Amersham Pharm Biotech Ab Sequencing systems
WO2000040334A1 (en) 1999-01-08 2000-07-13 Pe Corporation (Ny) Fiber array for contacting chemical species and methods for using and making same
JP2003526328A (ja) 1999-01-11 2003-09-09 プレジデント・アンド・フェロウズ・オブ・ハーバード・カレッジ Dnaの等温増幅
WO2000042233A1 (en) 1999-01-13 2000-07-20 Cornell Research Foundation, Inc. Monolithic fabrication of fluidic structures
ATE508200T1 (de) 1999-02-23 2011-05-15 Caliper Life Sciences Inc Sequenzierung durch inkorporation
AU3174600A (en) 1999-03-10 2000-09-28 Asm Scientific, Inc. A method for direct nucleic acid sequencing
US6515751B1 (en) 1999-03-11 2003-02-04 Cornell Research Foundation Inc. Mechanically resonant nanostructures
WO2000058507A1 (en) 1999-03-30 2000-10-05 Solexa Ltd. Polynucleotide sequencing
GB9907812D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Sequencing
GB9907813D0 (en) 1999-04-06 1999-06-02 Medical Biosystems Ltd Synthesis
US6355431B1 (en) 1999-04-20 2002-03-12 Illumina, Inc. Detection of nucleic acid amplification reactions using bead arrays
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US6056661A (en) 1999-06-14 2000-05-02 General Motors Corporation Multi-range transmission with input split planetary gear set and continuously variable transmission unit
US6818395B1 (en) 1999-06-28 2004-11-16 California Institute Of Technology Methods and apparatus for analyzing polynucleotide sequences
CA2381361A1 (en) 1999-08-13 2001-02-22 U.S. Genomics, Inc. Methods and apparatuses for stretching polymers
WO2001016375A2 (en) 1999-08-30 2001-03-08 The Government Of The United States Of America, As Represented By The Secretary, Department Of Health And Human Services High speed parallel molecular nucleic acid sequencing
US7244559B2 (en) 1999-09-16 2007-07-17 454 Life Sciences Corporation Method of sequencing a nucleic acid
US6274320B1 (en) 1999-09-16 2001-08-14 Curagen Corporation Method of sequencing a nucleic acid
US7211390B2 (en) 1999-09-16 2007-05-01 454 Life Sciences Corporation Method of sequencing a nucleic acid
WO2001023610A2 (en) 1999-09-29 2001-04-05 Solexa Ltd. Polynucleotide sequencing
GB9923644D0 (en) 1999-10-06 1999-12-08 Medical Biosystems Ltd DNA sequencing
AU2573801A (en) 1999-11-02 2001-05-14 University Of Hawaii Method for fabricating arrays of micro-needles
WO2001032930A1 (en) 1999-11-04 2001-05-10 California Institute Of Technology Methods and apparatuses for analyzing polynucleotide sequences
US6399335B1 (en) 1999-11-16 2002-06-04 Advanced Research And Technology Institute, Inc. γ-phosphoester nucleoside triphosphates
US6324327B1 (en) 1999-11-23 2001-11-27 Corning Incorporated Low dispersion slope negative dispersion optical fiber
CN1336960A (zh) * 1999-11-26 2002-02-20 株式会社百尼尔 采用多种dna聚合酶的dna测序方法和所用的试剂盒
EP1238105A2 (en) 1999-12-02 2002-09-11 Molecular Staging Inc. Generation of single-strand circular dna from linear self-annealing segments
DE60025850T2 (de) 1999-12-29 2006-08-03 Keygene N.V. Verfahren zur erzeugung von oligonukleotiden, im besonderen zur detektion amplifizierter restriktionsfragmente, die durch aflp erhalten wurden
GB0000896D0 (en) 2000-01-14 2000-03-08 Univ Glasgow Improved analytical chip
US6510263B1 (en) 2000-01-27 2003-01-21 Unaxis Balzers Aktiengesellschaft Waveguide plate and process for its production and microtitre plate
GB0002389D0 (en) 2000-02-02 2000-03-22 Solexa Ltd Molecular arrays
US6916665B2 (en) * 2000-02-11 2005-07-12 The Texas A&M University System Biosensor compositions and methods of use
DE10013254A1 (de) 2000-03-17 2001-10-04 Friz Biochem Gmbh Vorrichtung und Verfahren zum Nachweis organischer Moleküle in einer Probensubstanz
US20030213771A1 (en) 2000-03-31 2003-11-20 Kenichirou Ohshita Surface treatment method for magnesium alloys and magnesium alloy members thus treated
AU2001261358B2 (en) * 2000-05-10 2006-07-13 Board Of Supervisors Of Louisiana State University And Agricultural And Mechanical College Resistance to acetohydroxyacid synthase-inhibiting herbicides
US6291187B1 (en) * 2000-05-12 2001-09-18 Molecular Staging, Inc. Poly-primed amplification of nucleic acid sequences
KR100867760B1 (ko) 2000-05-15 2008-11-10 소니 가부시끼 가이샤 재생장치, 재생방법 및 기록매체
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
US20030027308A1 (en) 2000-05-30 2003-02-06 Sugen Incorporated Novel human protein phosphatases identified from genomic sequencing
US7396675B2 (en) 2000-06-02 2008-07-08 Bayer Technology Services Gmbh Kit and method for determining a plurality of analytes
EP1287154A4 (en) 2000-06-07 2010-12-15 Pacific Biosciences California NUCLEOTIDES WITH CHARGE SWITCHING
US6323009B1 (en) 2000-06-28 2001-11-27 Molecular Staging, Inc. Multiply-primed amplification of nucleic acid sequences
GB0016472D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method and apparatus
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
EP2100971A3 (en) * 2000-07-07 2009-11-25 Visigen Biotechnologies, Inc. Real-time sequence determination
EP1322936A2 (en) 2000-10-03 2003-07-02 California Institute Of Technology Microfluidic devices and methods of use
AU2002241747A1 (en) 2000-10-26 2002-06-18 The Trustees Of Princeton University, Princeton University Method and apparatus for dielectric spectroscopy of biological solutions
TWI316964B (pt) * 2000-10-30 2009-11-11 Takara Bio Inc
US20040157306A1 (en) 2000-11-13 2004-08-12 Sugen Incorporated Mammalian protein phosphatases
US7211414B2 (en) 2000-12-01 2007-05-01 Visigen Biotechnologies, Inc. Enzymatic nucleic acid synthesis: compositions and methods for altering monomer incorporation fidelity
GB2390155B (en) 2001-01-03 2004-04-21 Packard Instrument Co Inc Luminescence imager
US20020197618A1 (en) * 2001-01-20 2002-12-26 Sampson Jeffrey R. Synthesis and amplification of unstructured nucleic acids for rapid sequencing
DE10102578C2 (de) 2001-01-20 2003-01-09 Univ Braunschweig Tech Resonanter Mikrowellensensor
EP1356119B1 (en) 2001-01-30 2007-09-26 Solexa Ltd. The preparation of polynucleotide arrays
JP2002237200A (ja) 2001-02-13 2002-08-23 Mitsubishi Electric Corp 半導体装置およびその検査方法
JP2004523243A (ja) 2001-03-12 2004-08-05 カリフォルニア インスティチュート オブ テクノロジー 非同期性塩基伸長によってポリヌクレオチド配列を分析するための方法および装置
US20030143556A1 (en) 2001-04-03 2003-07-31 Gary Blackburn Nucleic acid reactions using labels with different redox potentials
US20040110180A1 (en) 2001-04-06 2004-06-10 Shirley Recipon Kinases and phosphatases
US20040259082A1 (en) 2001-04-24 2004-12-23 Li-Cor, Inc. Polymerases with charge-switch activity and methods of generating such polymers
CA2344599C (en) 2001-05-07 2011-07-12 Bioneer Corporation Selective polymerase chain reaction of dna of which base sequence is completely unknown
GB0112238D0 (en) 2001-05-18 2001-07-11 Medical Biosystems Ltd Sequencing method
WO2002094846A2 (en) 2001-05-22 2002-11-28 Parallel Synthesis Technologies, Inc. Method for in situ, on-chip chemical synthesis
US20040203097A1 (en) 2001-05-24 2004-10-14 Henry Yue Kinases and phosphatases
US7118907B2 (en) 2001-06-06 2006-10-10 Li-Cor, Inc. Single molecule detection systems and methods
US20020187508A1 (en) 2001-06-08 2002-12-12 Wong Gordon G. Methods and products for analyzing nucleic acids using nick translation
EP1417474B1 (en) 2001-07-25 2021-12-29 The Trustees Of Princeton University Nanochannel arrays and their preparation and use for high throughput macromolecular analysis
GB0119719D0 (en) 2001-08-13 2001-10-03 Solexa Ltd DNA sequence analysis
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
WO2003020734A2 (en) 2001-08-29 2003-03-13 Amersham Biosciences Corp Labeled nucleoside polyphosphates
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US6852492B2 (en) * 2001-09-24 2005-02-08 Intel Corporation Nucleic acid sequencing by raman monitoring of uptake of precursors during molecular replication
US6617137B2 (en) 2001-10-15 2003-09-09 Molecular Staging Inc. Method of amplifying whole genomes without subjecting the genome to denaturing conditions
AU2002343792A1 (en) * 2001-11-28 2003-06-10 Center For Advanced Science And Technology Incubation, Ltd. siRNA EXPRESSION SYSTEM AND PROCESS FOR PRODUCING FUNCTIONAL GENE-KNOCKDOWN CELLS AND THE LIKE USING THE SAME
US6713672B1 (en) 2001-12-07 2004-03-30 Laird Technologies, Inc. Compliant shaped EMI shield
JP2003185569A (ja) 2001-12-14 2003-07-03 Mitsubishi Chemicals Corp 表面プラズモン共鳴を利用した試料の分析装置及び表面プラズモン共鳴分析用センサチップ
US20030123827A1 (en) 2001-12-28 2003-07-03 Xtalight, Inc. Systems and methods of manufacturing integrated photonic circuit devices
JP3897703B2 (ja) 2002-01-11 2007-03-28 キヤノン株式会社 センサ装置およびそれを用いた検査方法
US6618537B2 (en) 2002-01-14 2003-09-09 Applied Wdm, Inc. Optical waveguide structures and methods of fabrication
US7422851B2 (en) 2002-01-31 2008-09-09 Nimblegen Systems, Inc. Correction for illumination non-uniformity during the synthesis of arrays of oligomers
MXPA03008967A (es) * 2002-02-01 2004-02-12 King Pharmaceuticals Res & Dev Antagonistas receptores de 8-heteroarilo xantina adenosina a-2b.
DE10217568A1 (de) 2002-04-19 2003-11-13 Infineon Technologies Ag Wellenleiter in porösen Substraten
US6815167B2 (en) 2002-04-25 2004-11-09 Geneohm Sciences Amplification of DNA to produce single-stranded product of defined sequence and length
US20050019784A1 (en) * 2002-05-20 2005-01-27 Xing Su Method and apparatus for nucleic acid sequencing and identification
JP2003344433A (ja) 2002-05-22 2003-12-03 Okutekku:Kk マイクロアレイ、マイクロアレイシステム及び被検物質の測定方法
US6977153B2 (en) 2002-12-31 2005-12-20 Qiagen Gmbh Rolling circle amplification of RNA
DE602004026033D1 (de) * 2003-01-29 2010-04-29 454 Corp Zweiendige sequenzierung
US7575865B2 (en) 2003-01-29 2009-08-18 454 Life Sciences Corporation Methods of amplifying and sequencing nucleic acids
US20040152119A1 (en) 2003-02-05 2004-08-05 Anup Sood Solid phase sequencing
CA2515938A1 (en) 2003-02-12 2004-08-26 Genizon Svenska Ab Methods and means for nucleic acid sequencing
GB2398383B (en) 2003-02-12 2005-03-09 Global Genomics Ab Method and means for nucleic acid sequencing
US7149396B2 (en) 2003-06-16 2006-12-12 The Regents Of The University Of California Apparatus for optical measurements on low-index non-solid materials based on arrow waveguides
WO2005030983A2 (en) * 2003-09-26 2005-04-07 Circleamp, Inc. Amplification of polynucleotides by rolling circle amplification
US7531306B2 (en) 2003-11-12 2009-05-12 Geneohm Sciences, Inc. Nucleic acid hybridization methods
US7169560B2 (en) 2003-11-12 2007-01-30 Helicos Biosciences Corporation Short cycle methods for sequencing polynucleotides
GB0400584D0 (en) 2004-01-12 2004-02-11 Solexa Ltd Nucleic acid chacterisation
JP2005218385A (ja) 2004-02-06 2005-08-18 Dnaform:Kk 1本鎖dnaを調製するための方法
ATE463584T1 (de) 2004-02-19 2010-04-15 Helicos Biosciences Corp Verfahren zur analyse von polynukleotidsequenzen
US20100216151A1 (en) * 2004-02-27 2010-08-26 Helicos Biosciences Corporation Methods for detecting fetal nucleic acids and diagnosing fetal abnormalities
WO2006028508A2 (en) * 2004-03-23 2006-03-16 President And Fellows Of Harvard College Methods and apparatus for characterizing polynucleotides
US7462452B2 (en) 2004-04-30 2008-12-09 Pacific Biosciences Of California, Inc. Field-switch sequencing
US7575863B2 (en) 2004-05-28 2009-08-18 Applied Biosystems, Llc Methods, compositions, and kits comprising linker probes for quantifying polynucleotides
US20060024711A1 (en) * 2004-07-02 2006-02-02 Helicos Biosciences Corporation Methods for nucleic acid amplification and sequence determination
US7256886B2 (en) 2004-07-22 2007-08-14 University Of Maryland At Baltimore County Surface enhanced Raman spectroscopic nano-imaging probe and uses therefor
US7315019B2 (en) 2004-09-17 2008-01-01 Pacific Biosciences Of California, Inc. Arrays of optical confinements and uses thereof
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US7424371B2 (en) 2004-12-21 2008-09-09 Helicos Biosciences Corporation Nucleic acid analysis
US7700287B2 (en) 2005-01-28 2010-04-20 Life Technologies Corporation Compositions and methods for terminating a sequencing reaction at a specific location in a target DNA template
JP4415266B2 (ja) 2005-02-01 2010-02-17 住友電装株式会社 シールドコネクタ
JP2006333739A (ja) 2005-05-31 2006-12-14 Hitachi High-Technologies Corp 単分子計測による核酸分析方法
WO2007145612A1 (en) 2005-06-06 2007-12-21 454 Life Sciences Corporation Paired end sequencing
KR20080047355A (ko) 2005-07-06 2008-05-28 바이오칩 이노베이션즈 피티와이 리미티드 표적 핵산 서열 분석 방법 및 키트
GB0514935D0 (en) 2005-07-20 2005-08-24 Solexa Ltd Methods for sequencing a polynucleotide template
US20070099212A1 (en) 2005-07-28 2007-05-03 Timothy Harris Consecutive base single molecule sequencing
US7405381B2 (en) 2005-08-23 2008-07-29 Cem, Corporation Real-time imaging and spectroscopy during microwave assisted chemistry
US7666593B2 (en) * 2005-08-26 2010-02-23 Helicos Biosciences Corporation Single molecule sequencing of captured nucleic acids
CN101535473A (zh) 2005-09-30 2009-09-16 加利福尼亚太平洋生物科学股份有限公司 反应性表面、基质及生产和利用它们的方法
US20070087358A1 (en) 2005-10-19 2007-04-19 Melanie Ehrlich Methods for diagnosing cancer based on DNA methylation status in NBL2
CA2631248C (en) 2005-11-28 2016-01-12 Pacific Biosciences Of California, Inc. Uniform surfaces for hybrid material substrates and methods for making and using same
US7998717B2 (en) 2005-12-02 2011-08-16 Pacific Biosciences Of California, Inc. Mitigation of photodamage in analytical reactions
JP2009519041A (ja) 2005-12-12 2009-05-14 アメリカ合衆国 核酸配列決定のためのプローブおよび使用方法
WO2007070642A2 (en) 2005-12-15 2007-06-21 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
JP2009521227A (ja) 2005-12-22 2009-06-04 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド ヌクレオチドアナログ組込み用ポリメラーゼ
CA2633520A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Protein engineering strategies to optimize activity of surface attached proteins
AU2006331512B2 (en) 2005-12-22 2012-02-23 Pacific Biosciences Of California, Inc. Active surface coupled polymerases
US7368265B2 (en) 2006-01-23 2008-05-06 Compass Genetics, Llc Selective genome amplification
US7715001B2 (en) 2006-02-13 2010-05-11 Pacific Biosciences Of California, Inc. Methods and systems for simultaneous real-time monitoring of optical signals from multiple sources
US20070196832A1 (en) * 2006-02-22 2007-08-23 Efcavitch J William Methods for mutation detection
US7608034B2 (en) * 2006-02-24 2009-10-27 Powis Parker Inc. Stack conditioning apparatus and method for use in bookbinding
CN101432439B (zh) 2006-02-24 2013-07-24 考利达基因组股份有限公司 Dna阵列上的高通量基因组测序
US8673567B2 (en) * 2006-03-08 2014-03-18 Atila Biosystems, Inc. Method and kit for nucleic acid sequence detection
US8975216B2 (en) 2006-03-30 2015-03-10 Pacific Biosciences Of California Articles having localized molecules disposed thereon and methods of producing same
US20090075252A1 (en) 2006-04-14 2009-03-19 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7282337B1 (en) * 2006-04-14 2007-10-16 Helicos Biosciences Corporation Methods for increasing accuracy of nucleic acid sequencing
US7897747B2 (en) 2006-05-25 2011-03-01 The Board Of Trustees Of The Leland Stanford Junior University Method to produce single stranded DNA of defined length and sequence and DNA probes produced thereby
AU2007260707A1 (en) 2006-06-16 2007-12-21 Pacific Biosciences Of California, Inc. Controlled initiation of primer extension
US7754429B2 (en) 2006-10-06 2010-07-13 Illumina Cambridge Limited Method for pair-wise sequencing a plurity of target polynucleotides
US20080108804A1 (en) * 2006-11-02 2008-05-08 Kabushiki Kaisha Dnaform Method for modifying RNAS and preparing DNAS from RNAS
US20090075343A1 (en) 2006-11-09 2009-03-19 Complete Genomics, Inc. Selection of dna adaptor orientation by nicking
US8182993B2 (en) 2007-06-06 2012-05-22 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
WO2009017678A2 (en) 2007-07-26 2009-02-05 Pacific Biosciences Of California, Inc. Molecular redundant sequencing
WO2009089384A1 (en) 2008-01-09 2009-07-16 Life Technologies Method of making a paired tag library for nucleic acid sequencing
US7767400B2 (en) 2008-02-03 2010-08-03 Helicos Biosciences Corporation Paired-end reads in sequencing by synthesis
US8143030B2 (en) 2008-09-24 2012-03-27 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
JP2011515102A (ja) 2008-03-28 2011-05-19 パシフィック バイオサイエンシーズ オブ カリフォルニア, インコーポレイテッド 核酸シーケンシング用組成物及び方法
WO2009124255A2 (en) 2008-04-04 2009-10-08 Helicos Biosciences Corporation Methods for transcript analysis
US20090269771A1 (en) 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
US9969608B2 (en) 2016-01-05 2018-05-15 DunAn Sensing, LLC Methods for mounting a MEMS sensor for in-stream measurements

Also Published As

Publication number Publication date
US20160348167A1 (en) 2016-12-01
EP3045542B1 (en) 2016-11-16
MX2010010600A (es) 2011-03-30
EP2274449B1 (en) 2015-12-23
EP2274449A2 (en) 2011-01-19
EP3425060A1 (en) 2019-01-09
US9600626B2 (en) 2017-03-21
AU2009229157A1 (en) 2009-10-01
US8153375B2 (en) 2012-04-10
CN102084001B (zh) 2015-03-18
US9542527B2 (en) 2017-01-10
US8309330B2 (en) 2012-11-13
EP4230747A2 (en) 2023-08-23
CA2991818A1 (en) 2009-10-01
EP4230747A3 (en) 2023-11-15
US9910956B2 (en) 2018-03-06
EP3045542A1 (en) 2016-07-20
CA2719747C (en) 2018-02-20
US20110281768A1 (en) 2011-11-17
EP3170904B1 (en) 2017-08-16
EP3170904A1 (en) 2017-05-24
US11705217B2 (en) 2023-07-18
US20160237485A1 (en) 2016-08-18
WO2009120372A2 (en) 2009-10-01
CA2719747A1 (en) 2009-10-01
US20090298075A1 (en) 2009-12-03
US20180211003A1 (en) 2018-07-26
WO2009120372A3 (en) 2010-01-07
US20160321397A1 (en) 2016-11-03
EP3269824A1 (en) 2018-01-17
CN104862383B (zh) 2019-05-28
US20260120801A1 (en) 2026-04-30
US20240212790A1 (en) 2024-06-27
US9404146B2 (en) 2016-08-02
EP3425060B1 (en) 2021-10-27
US20120289408A1 (en) 2012-11-15
CA2991818C (en) 2022-10-11
JP2011515102A (ja) 2011-05-19
CN102084001A (zh) 2011-06-01
US20160376647A1 (en) 2016-12-29
CN104862383A (zh) 2015-08-26
US9582640B2 (en) 2017-02-28
EP2274449A4 (en) 2012-04-18
AU2009229157B2 (en) 2015-01-29
US8455193B2 (en) 2013-06-04
US20130309678A1 (en) 2013-11-21
US20150159196A1 (en) 2015-06-11

Similar Documents

Publication Publication Date Title
ZA201102252B (en) Methods and kits for nucleic acid sequencing
DK3290528T3 (da) Fremgangsmåder og sammensætninger til nukleinsyresekventering
EP2201021A4 (en) METHOD OF ALTERNATING NUCLEIC ACID SEQUENCING
EP2274449A4 (en) COMPOSITIONS AND METHODS FOR NUCLEIC ACID SEQUENCING
EP3699291C0 (en) METHODS FOR ANALYZING UNIQUE NUCLEIC ACID SEQUENCE AND COMPOSITIONS
BRPI0920284A2 (pt) composição e método
BRPI0908748A2 (pt) Composições e métodos para análise de moléculas de ácido nucleico durante reações de amplificação
PL2285816T3 (pl) Sposób izolacji kwasów nukleinowych
ES2743029T8 (es) Composiciones de extremo del transposón y métodos para modificar ácidos nucleicos
BRPI0923225A2 (pt) metodo para sintese de acidos nucleicos modificados no atomo de fosforo
BRPI0906795A2 (pt) Método para produzir um l-aminoácido
BRPI0918299A2 (pt) Método para produzir um l-aminoácido
EP2427572A4 (en) SEQUENCING METHOD
EP1999276A4 (en) METHOD AND AGENT FOR NUCLEIC ACID SEQUENCING
DK1984502T3 (da) Nukleinsyre
EP2315619A4 (en) PROCESS FOR EXTRACTING NUCLEIC ACIDS
BR112012001100A2 (pt) aparelho e método para construções de estrutura e tijolo
BRPI0906489A2 (pt) Química e método de limpeza aprimorado por bolha
BRPI0912755A2 (pt) Método para produzir um l-aminoácido
BRPI0921959A2 (pt) métodos e composições para remoção de tatuagem
EP2327488A4 (en) SHEAR METHOD FOR PANEL
GB201014134D0 (en) Methods of RNA aplification in the presence of DNA
BRPI0914300A2 (pt) composições e métodos para imunogenicidade intensificada de somatostatina
ZA201102195B (en) Methods and compositions for isolating nucleic acid
BRPI0910286A2 (pt) métodos para transformação de levedura

Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B06I Publication of requirement cancelled [chapter 6.9 patent gazette]

Free format text: ANULADA A PUBLICACAO CODIGO 6.6.1 NA RPI NO 2480 DE 17/07/2018 POR TER SIDO INDEVIDA.

B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements