BRPI0911966A2 - Expressão heteróloga de celobioidrolases de fungo em levedura - Google Patents

Expressão heteróloga de celobioidrolases de fungo em levedura

Info

Publication number
BRPI0911966A2
BRPI0911966A2 BRPI0911966-3A BRPI0911966A BRPI0911966A2 BR PI0911966 A2 BRPI0911966 A2 BR PI0911966A2 BR PI0911966 A BRPI0911966 A BR PI0911966A BR PI0911966 A2 BRPI0911966 A2 BR PI0911966A2
Authority
BR
Brazil
Prior art keywords
yeast
heterologous expression
fungal cellobiohydrolases
cellobiohydrolases
fungal
Prior art date
Application number
BRPI0911966-3A
Other languages
English (en)
Inventor
Riaan Den Hann
Emile Van Zyl
Danie Lagrange
Original Assignee
Universiteit Stellenbosch
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Universiteit Stellenbosch filed Critical Universiteit Stellenbosch
Publication of BRPI0911966A2 publication Critical patent/BRPI0911966A2/pt
Publication of BRPI0911966B1 publication Critical patent/BRPI0911966B1/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02PCLIMATE CHANGE MITIGATION TECHNOLOGIES IN THE PRODUCTION OR PROCESSING OF GOODS
    • Y02P20/00Technologies relating to chemical industry
    • Y02P20/50Improvements relating to the production of bulk chemicals
    • Y02P20/52Improvements relating to the production of bulk chemicals using catalysts, e.g. selective catalysts

Landscapes

  • Chemical & Material Sciences (AREA)
  • Organic Chemistry (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • Biotechnology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Medicinal Chemistry (AREA)
  • Molecular Biology (AREA)
  • Biomedical Technology (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0911966A 2008-05-11 2009-05-11 polinucleotídeo codificando uma celobioidrolase BRPI0911966B1 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US5221308P 2008-05-11 2008-05-11
PCT/IB2009/005881 WO2009138877A2 (en) 2008-05-11 2009-05-11 Heterologous expression of fungal cellobiohydrolases in yeast

Publications (2)

Publication Number Publication Date
BRPI0911966A2 true BRPI0911966A2 (pt) 2015-07-28
BRPI0911966B1 BRPI0911966B1 (pt) 2018-11-13

Family

ID=41151793

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0911966A BRPI0911966B1 (pt) 2008-05-11 2009-05-11 polinucleotídeo codificando uma celobioidrolase

Country Status (4)

Country Link
US (2) US9365842B2 (pt)
BR (1) BRPI0911966B1 (pt)
CA (2) CA2724076C (pt)
WO (1) WO2009138877A2 (pt)

Families Citing this family (26)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2724076C (en) 2008-05-11 2018-11-27 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
WO2009146464A2 (en) * 2008-05-30 2009-12-03 Edenspace Systems Corporation Methods and compositions for modifying levels of lignin and increasing the digestibility of cellulose by the recombinant overexpression of lignocellulolytic enzymes
ES2621181T3 (es) 2008-11-21 2017-07-03 Lallemand Hungary Liquidity Management Llc Levaduras que expresan celulasas para la sacarificación y fermentación simultáneas utilizando celulosa
US9447398B2 (en) 2009-10-26 2016-09-20 Stellenbosch University Heterologous expression of fungal cellobiohydrolase 2 genes in yeast
US9133448B2 (en) 2010-02-11 2015-09-15 Dsm Ip Assets B.V. Polypeptide having cellobiohydrolase activity and uses thereof
EP2357227B1 (en) * 2010-02-11 2015-07-15 Süd-Chemie IP GmbH & Co. KG Optimized cellulase enzymes
US9605269B2 (en) 2010-05-05 2017-03-28 Lallemand Hungary Liquidity Management Llc Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
CN102884086B (zh) * 2010-06-01 2015-11-25 加州理工学院 稳定的功能嵌合纤维二糖水解酶i类酶
US9096871B2 (en) * 2010-10-06 2015-08-04 Bp Corporation North America Inc. Variant CBH I polypeptides with reduced product inhibition
EP2668269B1 (en) 2011-01-26 2018-01-03 Novozymes A/S Polypeptides having endoglucanase activity and polynucleotides encoding same
EP2668270B1 (en) 2011-01-26 2018-11-21 Novozymes A/S Polypeptides having cellobiohydrolase activity and polynucleotides encoding same
BR112013019039A2 (pt) 2011-01-26 2020-07-21 Novozymes A/S Célula hospedeira microbiana transgênica, métodos para produzir um polipeptídeo, para produzir um mutante de uma célula originária, para produzir uma proteína, para degradar um material celulósico, para produzir um produto de fermentação, 5 e parafermentar um material celulósico, construção de ácido nucleico ou vetor de expressão, polipeptídeo isolado, e polinucleotídeo isolado
MX2013007720A (es) * 2011-01-26 2013-08-09 Novozymes As Polipeptidos que tienen actividad celobiohidrolasa y polinucleotidos que codifican para los mismos.
EP3483279A1 (en) 2011-04-05 2019-05-15 Lallemand Hungary Liquidity Management LLC Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
CA2878019A1 (en) 2011-12-20 2013-06-27 Novozymes, Inc. Cellobiohydrolase variants and polynucleotides encoding same
US20150197760A1 (en) * 2012-06-19 2015-07-16 Dsm Ip Assets B.V. Promoters for expressing a gene in a cell
WO2014093275A1 (en) 2012-12-12 2014-06-19 Danisco Us Inc. Variants of cellobiohydrolases
CN105229141A (zh) 2013-03-15 2016-01-06 拉勒曼德匈牙利流动管理有限责任公司 用于木质纤维素和相关低聚物的水解的β-葡糖苷酶的表达
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
EP3027741B1 (en) 2013-07-29 2019-10-23 Danisco US Inc. Gh61 enzyme variants
WO2015023989A1 (en) 2013-08-15 2015-02-19 Lallemand Hungary Liquidity Management Llc Methods for the improvement of product yield and production in a microorganism through glycerol recycling
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CA3064519A1 (en) 2017-05-23 2018-11-29 Lallemand Hungary Liquidity Management Llc Optimization of biomass-based fermentations
WO2023028521A1 (en) * 2021-08-24 2023-03-02 Inscripta, Inc. Genome-wide rationally-designed mutations leading to enhanced cellobiohydrolase i production in s. cerevisiae
GB202115470D0 (en) * 2021-10-27 2021-12-08 Univ Stellenbosch Recombinant saccharomyces cerevisiae strains for enzymatic hydrolysis of bioplastic polymers
EP4638768A2 (en) * 2022-12-19 2025-10-29 Novozymes A/S Processes for producing fermentation products using fiber-degrading enzymes with engineered yeast

Family Cites Families (10)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US4683202A (en) 1985-03-28 1987-07-28 Cetus Corporation Process for amplifying nucleic acid sequences
WO1992003560A1 (en) 1990-08-28 1992-03-05 E.I. Du Pont De Nemours And Company A method for rapid selection of efficient secretion vectors
EP0644938A1 (en) 1992-05-29 1995-03-29 E.I. Du Pont De Nemours And Company PRODUCTION OF STREPTAVIDIN FROM $i(BACILLUS SUBTILIS)
DK2295544T3 (en) * 2001-06-26 2016-09-26 Novozymes As Polypeptides with cellobiohydrolase I activity and the same coding polynucleotides
EP2385104A1 (en) * 2003-04-01 2011-11-09 Danisco US Inc. Variant Scytalidium thermophilium CBH1
US8097445B2 (en) 2004-03-25 2012-01-17 Danisco Us Inc. Exo-endo cellulase fusion protein
NZ595497A (en) 2006-02-10 2013-09-27 Verenium Corp Cellulolytic enzymes, nucleic acids encoding them and methods for making and using them
CA2670102A1 (en) * 2006-11-22 2008-05-29 The Trustees Of Dartmouth College Recombinant yeast strains expressing tethered cellulase enzymes
CA2724076C (en) 2008-05-11 2018-11-27 Universiteit Stellenbosch Heterologous expression of fungal cellobiohydrolases in yeast
US8470592B2 (en) * 2008-07-07 2013-06-25 Mascoma Corporation Isolation and characterization of Schizochytrium aggregatum cellobiohydrolase I (Cbh 1)

Also Published As

Publication number Publication date
WO2009138877A2 (en) 2009-11-19
CA2724076C (en) 2018-11-27
US11530428B2 (en) 2022-12-20
CA2724076A1 (en) 2009-11-19
WO2009138877A3 (en) 2010-01-07
CA3021166A1 (en) 2009-11-19
CA3021166C (en) 2024-01-09
BRPI0911966B1 (pt) 2018-11-13
US9365842B2 (en) 2016-06-14
US20160326560A1 (en) 2016-11-10
US20110124074A1 (en) 2011-05-26

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Legal Events

Date Code Title Description
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B06A Patent application procedure suspended [chapter 6.1 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 11/05/2009, OBSERVADAS AS CONDICOES LEGAIS.