BRPI0915828A2 - expressão heteróloga de celulases de cupim em levedura - Google Patents

expressão heteróloga de celulases de cupim em levedura

Info

Publication number
BRPI0915828A2
BRPI0915828A2 BRPI0915828A BRPI0915828A BRPI0915828A2 BR PI0915828 A2 BRPI0915828 A2 BR PI0915828A2 BR PI0915828 A BRPI0915828 A BR PI0915828A BR PI0915828 A BRPI0915828 A BR PI0915828A BR PI0915828 A2 BRPI0915828 A2 BR PI0915828A2
Authority
BR
Brazil
Prior art keywords
yeast
heterologous expression
termite
cellulases
termite cellulases
Prior art date
Application number
BRPI0915828A
Other languages
English (en)
Inventor
Anne Warner
Chhayal Gandhi
Elena E Brevnova
Erin Wiswall
John Mcbride
Mark Mellon
Vineet Rajgarhia
Original Assignee
Mascoma Corp
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Mascoma Corp filed Critical Mascoma Corp
Publication of BRPI0915828A2 publication Critical patent/BRPI0915828A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N9/00Enzymes; Proenzymes; Compositions thereof; Processes for preparing, activating, inhibiting, separating or purifying enzymes
    • C12N9/14Hydrolases (3)
    • C12N9/24Hydrolases (3) acting on glycosyl compounds (3.2)
    • C12N9/2402Hydrolases (3) acting on glycosyl compounds (3.2) hydrolysing O- and S- glycosyl compounds (3.2.1)
    • C12N9/2405Glucanases
    • C12N9/2434Glucanases acting on beta-1,4-glucosidic bonds
    • C12N9/2437Cellulases (3.2.1.4; 3.2.1.74; 3.2.1.91; 3.2.1.150)
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12NMICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
    • C12N15/00Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
    • C12N15/09Recombinant DNA-technology
    • C12N15/63Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
    • C12N15/79Vectors or expression systems specially adapted for eukaryotic hosts
    • C12N15/80Vectors or expression systems specially adapted for eukaryotic hosts for fungi
    • C12N15/81Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts
    • C12N15/815Vectors or expression systems specially adapted for eukaryotic hosts for fungi for yeasts for yeasts other than Saccharomyces
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/02Monosaccharides
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P19/00Preparation of compounds containing saccharide radicals
    • C12P19/14Preparation of compounds containing saccharide radicals produced by the action of a carbohydrase (EC 3.2.x), e.g. by alpha-amylase, e.g. by cellulase, hemicellulase
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12PFERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIRED CHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERS FROM A RACEMIC MIXTURE
    • C12P7/00Preparation of oxygen-containing organic compounds
    • C12P7/02Preparation of oxygen-containing organic compounds containing a hydroxy group
    • C12P7/04Preparation of oxygen-containing organic compounds containing a hydroxy group acyclic
    • C12P7/06Ethanol, i.e. non-beverage
    • C12P7/08Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate
    • C12P7/10Ethanol, i.e. non-beverage produced as by-product or from waste or cellulosic material substrate substrate containing cellulosic material
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12YENZYMES
    • C12Y302/00Hydrolases acting on glycosyl compounds, i.e. glycosylases (3.2)
    • C12Y302/01Glycosidases, i.e. enzymes hydrolysing O- and S-glycosyl compounds (3.2.1)
    • C12Y302/01091Cellulose 1,4-beta-cellobiosidase (3.2.1.91)
    • YGENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
    • Y02TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
    • Y02EREDUCTION OF GREENHOUSE GAS [GHG] EMISSIONS, RELATED TO ENERGY GENERATION, TRANSMISSION OR DISTRIBUTION
    • Y02E50/00Technologies for the production of fuel of non-fossil origin
    • Y02E50/10Biofuels, e.g. bio-diesel

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Organic Chemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Genetics & Genomics (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Engineering & Computer Science (AREA)
  • Microbiology (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Biomedical Technology (AREA)
  • Mycology (AREA)
  • Molecular Biology (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • General Chemical & Material Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biophysics (AREA)
  • Plant Pathology (AREA)
  • Medicinal Chemistry (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)
BRPI0915828A 2008-07-07 2009-07-07 expressão heteróloga de celulases de cupim em levedura BRPI0915828A2 (pt)

Applications Claiming Priority (2)

Application Number Priority Date Filing Date Title
US7873508P 2008-07-07 2008-07-07
PCT/US2009/003970 WO2010005551A2 (en) 2008-07-07 2009-07-07 Heterologous expression of termite cellulases in yeast

Publications (1)

Publication Number Publication Date
BRPI0915828A2 true BRPI0915828A2 (pt) 2015-11-03

Family

ID=41100558

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0915828A BRPI0915828A2 (pt) 2008-07-07 2009-07-07 expressão heteróloga de celulases de cupim em levedura

Country Status (6)

Country Link
US (5) US8658398B2 (pt)
CN (1) CN102143972A (pt)
BR (1) BRPI0915828A2 (pt)
CA (1) CA2729945A1 (pt)
WO (1) WO2010005551A2 (pt)
ZA (1) ZA201100148B (pt)

Families Citing this family (28)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
CA2729945A1 (en) * 2008-07-07 2010-01-14 Mascoma Corporation Heterologous expression of termite cellulases in yeast
ES2621181T3 (es) 2008-11-21 2017-07-03 Lallemand Hungary Liquidity Management Llc Levaduras que expresan celulasas para la sacarificación y fermentación simultáneas utilizando celulosa
US8445240B2 (en) * 2009-04-10 2013-05-21 University Of Florida Research Foundation, Inc. Termite enzymes and uses thereof for in vitro conversion of lignin-containing materials to fermentable products
CN102666849A (zh) 2009-10-26 2012-09-12 斯泰伦博斯大学 真菌纤维二糖水解酶2基因在酵母中的异源表达
US8772012B2 (en) 2009-12-22 2014-07-08 Kabushiki Kaisha Toyota Chuo Kenkyusho Xylose isomerase and use thereof
WO2011140386A2 (en) 2010-05-05 2011-11-10 Mascoma Corporation Detoxification of biomass derived acetate via metabolic conversion to ethanol, acetone, isopropanol, or ethyl acetate
JPWO2012060389A1 (ja) * 2010-11-05 2014-05-12 旭硝子株式会社 シゾサッカロミセス属酵母の形質転換体およびその製造方法
BR112013022047B1 (pt) 2011-02-28 2020-11-17 Cadena Bio, Inc catalisadores de ácidos poliméricos e seus usos
CA2832279C (en) 2011-04-05 2024-03-05 Mascoma Corporation Methods for the improvement of product yield and production in a microorganism through the addition of alternate electron acceptors
EP2744898A1 (en) * 2011-08-15 2014-06-25 Novozymes A/S Polypeptides having cellulase activity and polynucleotides encoding same
US20140024064A1 (en) 2012-07-23 2014-01-23 Butamax(Tm) Advanced Biofuels Llc Processes and systems for the production of fermentative alcohols
US9238845B2 (en) 2012-08-24 2016-01-19 Midori Usa, Inc. Methods of producing sugars from biomass feedstocks
BR112015005439A8 (pt) 2012-09-12 2022-01-11 Butamax Advanced Biofuels Llc Método para recuperar um produto alcoólico de um caldo de fermentação e sistema
CA2887574A1 (en) 2012-10-11 2014-04-17 Butamax Advanced Biofuels Llc Processes and systems for the production of fermentation products
CN103773703A (zh) * 2012-10-23 2014-05-07 贵州爱微生物技术有限责任公司 一种基因重组纤维素分解酵母菌方法
US20150337347A1 (en) * 2012-11-30 2015-11-26 Novozymes A/S Polypeptides Having Cellulolytic Enhancing Activity And Polynucleotides Encoding Same
US9850512B2 (en) 2013-03-15 2017-12-26 The Research Foundation For The State University Of New York Hydrolysis of cellulosic fines in primary clarified sludge of paper mills and the addition of a surfactant to increase the yield
DK3027742T3 (da) 2013-07-29 2020-02-24 Danisco Us Inc Enzymvarianter
DK3033413T4 (da) 2013-08-15 2023-07-03 Lallemand Hungary Liquidity Man Llc Fremgangsmåder til forbedring af produktudbytte og produktion i en mikroorganisme gennem recirkulering af glycerol
US9951363B2 (en) 2014-03-14 2018-04-24 The Research Foundation for the State University of New York College of Environmental Science and Forestry Enzymatic hydrolysis of old corrugated cardboard (OCC) fines from recycled linerboard mill waste rejects
CN104726432B (zh) * 2014-12-22 2017-12-22 江苏大学 一种D型β‑葡萄糖苷酶突变体及其表达质粒和重组菌
US20160353747A1 (en) * 2015-06-08 2016-12-08 Ento Bio LLC Compositions and methods for achieving a biological effect in target vegetation
CN105039190B (zh) * 2015-06-26 2018-12-18 中国环境科学研究院 一种降解纤维素的基因重组酿酒酵母及构建方法和应用
CN106591350B (zh) * 2016-12-20 2018-06-05 广州格拉姆生物科技有限公司 一种能降解纤维素生产益生纤维寡糖并分泌抗菌肽的多功能酿酒酵母
BR122022015725B1 (pt) 2017-05-23 2023-03-14 Lallemand Hungary Liquidity Management Llc Processo para a produção de um produto de fermentação
US11162087B2 (en) * 2017-08-30 2021-11-02 Riken Beta-glucosidase, enzyme composition including same, and method for manufacturing sugar solution using same
CN109652467A (zh) * 2019-02-19 2019-04-19 广西农垦糖业集团昌菱制糖有限公司 一种利用蔗渣生产乙醇的方法
CN119799530B (zh) * 2024-12-31 2025-12-16 溧阳市优甜生物科技有限公司 重组酵母细胞及其在生产索马甜中的用途

Family Cites Families (5)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
JP3030349B2 (ja) * 1997-07-31 2000-04-10 農林水産省蚕糸・昆虫農業技術研究所長 昆虫由来のセルラーゼ遺伝子
WO2003000941A2 (en) 2001-06-26 2003-01-03 Novozymes A/S Polypeptides having cellobiohydrolase i activity and polynucleotides encoding same
JP4224601B2 (ja) * 2001-09-03 2009-02-18 独立行政法人科学技術振興機構 シロアリ共生原生動物由来のセルラーゼ遺伝子
CN101245343A (zh) * 2007-02-13 2008-08-20 中国科学院上海生命科学研究院 新的纤维素酶及其应用
CA2729945A1 (en) * 2008-07-07 2010-01-14 Mascoma Corporation Heterologous expression of termite cellulases in yeast

Also Published As

Publication number Publication date
CN102143972A (zh) 2011-08-03
WO2010005551A2 (en) 2010-01-14
US20180155700A1 (en) 2018-06-07
WO2010005551A3 (en) 2010-03-18
US8658398B2 (en) 2014-02-25
US9856465B2 (en) 2018-01-02
CA2729945A1 (en) 2010-01-14
ZA201100148B (en) 2020-12-23
US10214733B2 (en) 2019-02-26
US20120003701A1 (en) 2012-01-05
US20190127714A1 (en) 2019-05-02
US20150024438A1 (en) 2015-01-22
US10428322B2 (en) 2019-10-01
US20160177283A1 (en) 2016-06-23

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Legal Events

Date Code Title Description
B25A Requested transfer of rights approved

Owner name: LALLEMAND HUNGARY LIQUIDITY MANAGEMENT LLC. (HU)

B06F Objections, documents and/or translations needed after an examination request according art. 34 industrial property law
B07D Technical examination (opinion) related to article 229 of industrial property law

Free format text: DE ACORDO COM O ARTIGO 229-C DA LEI NO 10196/2001

B07G Grant request does not fulfill article 229-c lpi (prior consent of anvisa)
B06U Preliminary requirement: requests with searches performed by other patent offices: suspension of the patent application procedure
B11B Dismissal acc. art. 36, par 1 of ipl - no reply within 90 days to fullfil the necessary requirements