BRPI0921518A2 - método e dispositivo de citometria de fluxo sem fluidos envolventes - Google Patents

método e dispositivo de citometria de fluxo sem fluidos envolventes

Info

Publication number
BRPI0921518A2
BRPI0921518A2 BRPI0921518-2A BRPI0921518A BRPI0921518A2 BR PI0921518 A2 BRPI0921518 A2 BR PI0921518A2 BR PI0921518 A BRPI0921518 A BR PI0921518A BR PI0921518 A2 BRPI0921518 A2 BR PI0921518A2
Authority
BR
Brazil
Prior art keywords
flow cytometry
particles
cytometry method
surrounding fluid
relates
Prior art date
Application number
BRPI0921518-2A
Other languages
English (en)
Inventor
Champseix Henri
Magnin Oliver
Welmant Bernard
Original Assignee
C2 Diagnostics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by C2 Diagnostics filed Critical C2 Diagnostics
Publication of BRPI0921518A2 publication Critical patent/BRPI0921518A2/pt
Publication of BRPI0921518B1 publication Critical patent/BRPI0921518B1/pt
Publication of BRPI0921518B8 publication Critical patent/BRPI0921518B8/pt

Links

Classifications

    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/1031Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G01N15/131Details
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/1023Microstructural devices for non-optical measurement
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/1031Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G01N15/131Details
    • G01N15/132Circuits
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404Handling flow, e.g. hydrodynamic focusing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • G01N15/1433Signal processing using image recognition
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483Physical analysis of biological material
    • G01N33/487Physical analysis of biological material of liquid biological material
    • G01N33/49Blood
    • G01N33/4915Blood using flow cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429Signal processing
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N15/14Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484Optical investigation techniques, e.g. flow cytometry microstructural devices
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016White blood cells
    • GPHYSICS
    • G01MEASURING; TESTING
    • G01NINVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10Investigating individual particles
    • G01N2015/1019Associating Coulter-counter and optical flow cytometer [OFC]

Landscapes

  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Biochemistry (AREA)
  • Analytical Chemistry (AREA)
  • General Health & Medical Sciences (AREA)
  • General Physics & Mathematics (AREA)
  • Immunology (AREA)
  • Pathology (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Molecular Biology (AREA)
  • Urology & Nephrology (AREA)
  • Food Science & Technology (AREA)
  • Medicinal Chemistry (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Measuring Cells (AREA)
BRPI0921518A 2008-12-02 2009-12-01 método e dispositivo de citometria de fluxo sem fluidos envolventes BRPI0921518B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0858200 2008-12-02
FR0858200A FR2939199B1 (fr) 2008-12-02 2008-12-02 Procede et dispositif de cytometrie en flux sans fluide de gainage
PCT/FR2009/052349 WO2010063936A1 (fr) 2008-12-02 2009-12-01 Procede et dispositif de cytometrie en flux sans fluide de gainage

Publications (3)

Publication Number Publication Date
BRPI0921518A2 true BRPI0921518A2 (pt) 2018-05-29
BRPI0921518B1 BRPI0921518B1 (pt) 2019-06-04
BRPI0921518B8 BRPI0921518B8 (pt) 2019-10-15

Family

ID=40800454

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0921518A BRPI0921518B8 (pt) 2008-12-02 2009-12-01 método e dispositivo de citometria de fluxo sem fluidos envolventes

Country Status (10)

Country Link
US (1) US9243992B2 (pt)
EP (1) EP2352984B1 (pt)
JP (1) JP2012510627A (pt)
CN (1) CN102239400B (pt)
BR (1) BRPI0921518B8 (pt)
FR (1) FR2939199B1 (pt)
MX (1) MX2011005656A (pt)
PL (1) PL2352984T3 (pt)
RU (1) RU2511065C2 (pt)
WO (1) WO2010063936A1 (pt)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
USD673287S1 (en) 2010-11-24 2012-12-25 Sony Corporation Micro flow channel chip
USD869308S1 (en) 2010-04-29 2019-12-10 Sony Corporation Micro flow channel chip
USD704580S1 (en) 2012-03-08 2014-05-13 Sony Corporation Micro flow channel chip for flow cytometer
DE102010024964B4 (de) * 2010-06-24 2012-01-26 Siemens Aktiengesellschaft Zellüberwachung mittels Streulichtmessung
CN103323395A (zh) * 2013-06-27 2013-09-25 中国科学院苏州生物医学工程技术研究所 一种应用于流式分析仪的单光源多通道光学系统
CN104634719A (zh) * 2014-12-29 2015-05-20 中国科学院长春光学精密机械与物理研究所 一种流式细胞仪的流动室
JP6438319B2 (ja) * 2015-02-18 2018-12-12 アズビル株式会社 粒子検出装置
JP6557022B2 (ja) * 2015-02-18 2019-08-07 アズビル株式会社 粒子検出装置
US10527538B2 (en) * 2015-05-28 2020-01-07 Accellix Ltd. System and apparatus for blind deconvolution of flow cytometer particle emission
EP3315963A1 (de) * 2016-10-26 2018-05-02 Fuchs Petrolub SE Probenaufnahmeelement, analysenset und verfahren zur analyse eines liquids, insbesondere einer kühlschmierstoffemulsion
CN114047111B (zh) * 2016-11-07 2025-09-26 芯易诊有限公司 样品分析方法
US11215545B2 (en) 2017-06-14 2022-01-04 Cytochip Inc. Devices and methods for cell analysis
FR3068469B1 (fr) * 2017-06-28 2020-09-11 Diagdev Cuve de mesure pour le denombrement et/ou la caracterisation de cellules
PL3685142T3 (pl) * 2017-09-21 2025-06-09 Bit Group France Optyczny cytometr przepływowy do pomiaru epi-fluorescencji
WO2019083844A1 (en) 2017-10-23 2019-05-02 Cytochip Inc. DEVICES AND METHOD FOR MEASURING TARGET ANALYTES AND PARTICLES
CN108663306B (zh) * 2018-07-27 2024-02-09 北京指真生物科技有限公司 一种流式细胞分析装置及补偿方法
GB201815114D0 (en) * 2018-09-17 2018-10-31 Univ Southampton Impedance flow cytometry apparatus
CN109357992B (zh) * 2018-11-09 2021-11-02 赛默飞世尔(上海)仪器有限公司 用于对光束进行整形的光学系统及流式细胞仪
CA3076064A1 (en) * 2019-01-02 2020-07-02 M & J Scientific, Llc Light scattering detectors and sample cells for the same
WO2020142096A1 (en) 2019-01-02 2020-07-09 M & J Scientific, Llc Light scattering detectors and methods for the same
CN109765253B (zh) * 2019-01-04 2022-05-10 潍坊华鼎电子技术有限公司 一种增强器输出屏用荧光粉浆料的检测方法
EP3942279A4 (en) * 2019-03-21 2022-12-28 Becton, Dickinson and Company LIGHT DETECTION SYSTEMS AND METHODS OF USE THEREOF

Family Cites Families (34)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US2656508A (en) 1949-08-27 1953-10-20 Wallace H Coulter Means for counting particles suspended in a fluid
DE2166594A1 (de) * 1970-04-28 1975-01-09 Gen Science Corp Einrichtung zum zaehlen von partikeln in einem stroemungsmittel und verfahren zur selbsttaetigen schwellenwertkennlinienaufzeichnung fuer eine derartige einrichtung
BE774319A (fr) * 1970-10-27 1972-02-14 Coulter Electronics Peilinrichting voor peilen van de axiale banen voor het op elektronische wijze bestuderen van partikels en werkwijze daarvoor
US3710264A (en) * 1971-04-09 1973-01-09 Coulter Electronics Axial trajectory sensor having gating means controlled by pulse duration measuring for electronic particle study apparatus and method
US4420720A (en) * 1981-06-29 1983-12-13 Coulter Electronics, Inc. Field focused particle sensing zone
US4633169A (en) * 1983-07-08 1986-12-30 Kontron Holding Ag Blood particle analyzer
US4710021A (en) * 1983-10-14 1987-12-01 Sequoia-Turner Corporation Particulate matter analyzing apparatus and method
JPS60257342A (ja) * 1984-06-04 1985-12-19 Toa Medical Electronics Co Ltd 粒子分析装置
GB2177804A (en) 1985-05-31 1987-01-28 Coulter Electronics Analysing and editing electrical pulses
JPH0665979B2 (ja) * 1985-07-26 1994-08-24 東亜医用電子株式会社 光による粒子検出装置
FR2653885B1 (fr) * 1989-10-27 1994-01-14 Abx Appareil pour le comptage et la determination d'au moins une sous-population leucocytaire.
JPH06505099A (ja) * 1991-02-22 1994-06-09 クールター コーポレイション 偏光光散乱手法を用いて微視的細胞をスクリーニングする方法および装置
US5335668A (en) * 1993-04-30 1994-08-09 Medical Scientific, Inc. Diagnostic impedance measuring system for an insufflation needle
US5812419A (en) 1994-08-01 1998-09-22 Abbott Laboratories Fully automated analysis method with optical system for blood cell analyzer
JP3347495B2 (ja) * 1994-11-14 2002-11-20 シスメックス株式会社 粒子分析装置
GB9810492D0 (en) * 1998-05-16 1998-07-15 Microbial Systems Ltd Particle detection system and components thereof
US6228652B1 (en) 1999-02-16 2001-05-08 Coulter International Corp. Method and apparatus for analyzing cells in a whole blood sample
WO2001069202A2 (en) * 2000-03-16 2001-09-20 Ulrik Darling Larsen Sensor units for particle characterisation apparatus
US6624621B2 (en) * 2000-04-03 2003-09-23 Howard L. North, Jr. Particle counter volume sensor
US20020028434A1 (en) * 2000-09-06 2002-03-07 Guava Technologies, Inc. Particle or cell analyzer and method
GB0113905D0 (en) * 2001-06-07 2001-08-01 Peratech Ltd Analytical device
FR2865145B1 (fr) * 2004-01-19 2006-02-24 Commissariat Energie Atomique Dispositif de dispense de gouttelettes microfluidiques notamment pour la cytometrie.
US7440101B2 (en) * 2004-01-23 2008-10-21 Beckman Coulter, Inc. System and method for multiple laser triggering
US7208319B2 (en) * 2004-02-10 2007-04-24 Beckman Coulter, Inc. Method of measurement of nucleated red blood cells
FR2873813B1 (fr) 2004-07-30 2006-11-17 Abx Sa Procede et dispositif de caracterisation des composants cellulaires d'un liquide biologique
US20060160239A1 (en) * 2005-01-14 2006-07-20 Sung-Jae Lee Method of measuring a level of contamination in a chemical solution and systems thereof
ES2609412T3 (es) * 2005-02-18 2017-04-20 Hematologics, Inc. Sistema para detectar células anómalas empleando un análisis multidimensional
JP4745030B2 (ja) * 2005-11-15 2011-08-10 シスメックス株式会社 血液分析装置
US7788067B2 (en) * 2006-05-12 2010-08-31 Artium Technologies, Inc. Means and methods for signal validation for sizing spherical objects
CN101165485B (zh) * 2006-10-18 2012-09-19 深圳迈瑞生物医疗电子股份有限公司 一种血液细胞检测的装置及其微孔传感器组件
US7777872B2 (en) * 2007-07-31 2010-08-17 Alcon Research, Ltd. Method of measuring diffractive lenses
US8304245B2 (en) * 2007-11-02 2012-11-06 Children's Hospital And Research Center At Oakland Microfluidic flow lysometer device, system and method
US7817254B2 (en) * 2008-01-30 2010-10-19 Palo Alto Research Center Incorporated Obtaining information from time variation of sensing results
JP5671460B2 (ja) * 2008-08-07 2015-02-18 ユニバーシティ オブ マサチューセッツ 分光センサ

Also Published As

Publication number Publication date
CN102239400A (zh) 2011-11-09
MX2011005656A (es) 2011-06-17
BRPI0921518B1 (pt) 2019-06-04
EP2352984B1 (fr) 2019-05-22
WO2010063936A9 (fr) 2010-08-19
US20110235030A1 (en) 2011-09-29
CN102239400B (zh) 2017-10-10
BRPI0921518B8 (pt) 2019-10-15
WO2010063936A1 (fr) 2010-06-10
EP2352984A1 (fr) 2011-08-10
JP2012510627A (ja) 2012-05-10
FR2939199B1 (fr) 2011-02-11
US9243992B2 (en) 2016-01-26
FR2939199A1 (fr) 2010-06-04
RU2511065C2 (ru) 2014-04-10
RU2011127235A (ru) 2013-01-10
PL2352984T3 (pl) 2019-11-29

Similar Documents

Publication Publication Date Title
BRPI0921518A2 (pt) método e dispositivo de citometria de fluxo sem fluidos envolventes
PL398979A1 (pl) Urzadzenie mikroprzeplywowe i uklad mikroprzeplywowy obejmujacy jedno lub wiecej urzadzen mikroprzeplywowych
WO2008058217A3 (en) Flow cell for a flow cytometer system
AU2019268197A1 (en) Backflush methods and devices for chromatography
GB2504022A (en) Surface preparation
WO2015063552A3 (en) Microfluidic system and method with focused energy apparatus
WO2015081242A3 (en) Fluidics system for flow cytometer
EP1991863A4 (en) FLUIDIC SYSTEM FOR A FLOW CYTOMETER
WO2012075358A3 (en) Tubular array for fluidic focusing with integrated optical access region
MX2014007248A (es) Control de flujo de fluido.
EP4450163A3 (en) Modular point-of-care devices and uses thereof
WO2014152874A3 (en) Electrosurgical instrument
WO2011017681A3 (en) Device and method for molecular analysis
WO2014151209A8 (en) Dynamic aspiration methods and systems
WO2010104993A3 (en) Fluidic flow cytometry devices and particle sensing based on signal-encoding
MX386038B (es) Ensamble medidor de valvula y metodo.
MX2013005837A (es) Aparato de valvula.
EP2374540A3 (en) Chip for analyzing fluids being moved without an outside power source
EP2722274A3 (en) Flow control actuator with an adjustable frequency
EP4299959A3 (en) Fluid flow control devices and systems, and methods of flowing fluids therethrough
WO2013079927A3 (en) Air sampling device
MY163199A (en) Switching apparatus for a fluid flow
MX372822B (es) Dispositivo de preparación y extracción de muestras.
MX351927B (es) Control de flujo de fluido utilizando canales.
HK1208256A1 (en) Microfluidic pump

Legal Events

Date Code Title Description
B06T Formal requirements before examination [chapter 6.20 patent gazette]
B15K Others concerning applications: alteration of classification

Free format text: AS CLASSIFICACOES ANTERIORES ERAM: G01N 15/12 , G01N 15/14

Ipc: G01N 15/12 (1985.01), G01N 15/14 (1985.01), G01N 3

B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B09A Decision: intention to grant [chapter 9.1 patent gazette]
B16A Patent or certificate of addition of invention granted [chapter 16.1 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/12/2009, OBSERVADAS AS CONDICOES LEGAIS. (CO) 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/12/2009, OBSERVADAS AS CONDICOES LEGAIS

B16C Correction of notification of the grant [chapter 16.3 patent gazette]

Free format text: PRAZO DE VALIDADE: 20 (VINTE) ANOS CONTADOS A PARTIR DE 01/12/2009, OBSERVADAS AS CONDICOES LEGAIS. (CO) REFERENTE A RPI 2526 DE 04/06/2019, QUANTO AO ITEM (73) ENDERECO DO TITULAR.

B21F Lapse acc. art. 78, item iv - on non-payment of the annual fees in time

Free format text: REFERENTE A 14A ANUIDADE.

B24J Lapse because of non-payment of annual fees (definitively: art 78 iv lpi, resolution 113/2013 art. 12)

Free format text: EM VIRTUDE DA EXTINCAO PUBLICADA NA RPI 2751 DE 26-09-2023 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDA A EXTINCAO DA PATENTE E SEUS CERTIFICADOS, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013.