BRPI0921518B8 - método e dispositivo de citometria de fluxo sem fluidos envolventes - Google Patents

método e dispositivo de citometria de fluxo sem fluidos envolventes Download PDF

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Publication number
BRPI0921518B8
BRPI0921518B8 BRPI0921518A BRPI0921518A BRPI0921518B8 BR PI0921518 B8 BRPI0921518 B8 BR PI0921518B8 BR PI0921518 A BRPI0921518 A BR PI0921518A BR PI0921518 A BRPI0921518 A BR PI0921518A BR PI0921518 B8 BRPI0921518 B8 BR PI0921518B8
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BR
Brazil
Prior art keywords
flow cytometry
cytometry method
surrounding fluid
present
measurement area
Prior art date
Application number
BRPI0921518A
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English (en)
Inventor
Welmant Bernard
Champseix Henri
Magnin Oliver
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C2 Diagnostics
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
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Publication date
Application filed by C2 Diagnostics filed Critical C2 Diagnostics
Publication of BRPI0921518A2 publication Critical patent/BRPI0921518A2/pt
Publication of BRPI0921518B1 publication Critical patent/BRPI0921518B1/pt
Publication of BRPI0921518B8 publication Critical patent/BRPI0921518B8/pt

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    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G01N15/131—Details
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1023—Microstructural devices for non-optical measurement
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/1031—Investigating individual particles by measuring electrical or magnetic effects
    • G01N15/12—Investigating individual particles by measuring electrical or magnetic effects by observing changes in resistance or impedance across apertures when traversed by individual particles, e.g. by using the Coulter principle
    • G01N15/131—Details
    • G01N15/132—Circuits
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G01N15/1433—Signal processing using image recognition
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1456—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals
    • G01N15/1459—Optical investigation techniques, e.g. flow cytometry without spatial resolution of the texture or inner structure of the particle, e.g. processing of pulse signals the analysis being performed on a sample stream
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N33/00—Investigating or analysing materials by specific methods not covered by groups G01N1/00 - G01N31/00
    • G01N33/48—Biological material, e.g. blood, urine; Haemocytometers
    • G01N33/483—Physical analysis of biological material
    • G01N33/487—Physical analysis of biological material of liquid biological material
    • G01N33/49—Blood
    • G01N33/4915—Blood using flow cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1404—Handling flow, e.g. hydrodynamic focusing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1429—Signal processing
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N15/14—Optical investigation techniques, e.g. flow cytometry
    • G01N15/1484—Optical investigation techniques, e.g. flow cytometry microstructural devices
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/01—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials specially adapted for biological cells, e.g. blood cells
    • G01N2015/016—White blood cells
    • G—PHYSICS
    • G01—MEASURING; TESTING
    • G01N—INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIR CHEMICAL OR PHYSICAL PROPERTIES
    • G01N15/00—Investigating characteristics of particles; Investigating permeability, pore-volume or surface-area of porous materials
    • G01N15/10—Investigating individual particles
    • G01N2015/1019—Associating Coulter-counter and optical flow cytometer [OFC]

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  • Chemical & Material Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Physics & Mathematics (AREA)
  • Pathology (AREA)
  • Analytical Chemistry (AREA)
  • Immunology (AREA)
  • General Physics & Mathematics (AREA)
  • General Health & Medical Sciences (AREA)
  • Biochemistry (AREA)
  • Engineering & Computer Science (AREA)
  • Dispersion Chemistry (AREA)
  • Biomedical Technology (AREA)
  • Hematology (AREA)
  • Medicinal Chemistry (AREA)
  • Food Science & Technology (AREA)
  • Urology & Nephrology (AREA)
  • Molecular Biology (AREA)
  • Ecology (AREA)
  • Biophysics (AREA)
  • Signal Processing (AREA)
  • Investigating Or Analyzing Materials By The Use Of Electric Means (AREA)
  • Investigating Or Analysing Biological Materials (AREA)
  • Investigating Or Analysing Materials By Optical Means (AREA)
  • Optical Measuring Cells (AREA)

Abstract

método e dispositivo de citometria de fluxo sem fluidos envolventes a presente invenção refere-se a um método e a um dispositivo que adotam o método da citometria de fluxo, os quais podem ser usados, de preferência, mas sem a isto se restringir, na contagem e classificação de leucócitos. mais especificamente, ela se refere ao campo dos instrumentos de hematologia simplificados com custos operacionais moderados. o método de acordo com a presente invenção é caracterizado por usar uma técnica de medição de impedância para identificar as partículas cuja trajetória incluiu uma área de medição óptica predeterminada a fim de processá-las seletivamente, evitando assim o uso de fluidos envolventes para manter as partículas dentro da referida área de medição.
BRPI0921518A 2008-12-02 2009-12-01 método e dispositivo de citometria de fluxo sem fluidos envolventes BRPI0921518B8 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
FR0858200 2008-12-02
FR0858200A FR2939199B1 (fr) 2008-12-02 2008-12-02 Procede et dispositif de cytometrie en flux sans fluide de gainage
PCT/FR2009/052349 WO2010063936A1 (fr) 2008-12-02 2009-12-01 Procede et dispositif de cytometrie en flux sans fluide de gainage

Publications (3)

Publication Number Publication Date
BRPI0921518A2 BRPI0921518A2 (pt) 2018-05-29
BRPI0921518B1 BRPI0921518B1 (pt) 2019-06-04
BRPI0921518B8 true BRPI0921518B8 (pt) 2019-10-15

Family

ID=40800454

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0921518A BRPI0921518B8 (pt) 2008-12-02 2009-12-01 método e dispositivo de citometria de fluxo sem fluidos envolventes

Country Status (10)

Country Link
US (1) US9243992B2 (pt)
EP (1) EP2352984B1 (pt)
JP (1) JP2012510627A (pt)
CN (1) CN102239400B (pt)
BR (1) BRPI0921518B8 (pt)
FR (1) FR2939199B1 (pt)
MX (1) MX2011005656A (pt)
PL (1) PL2352984T3 (pt)
RU (1) RU2511065C2 (pt)
WO (1) WO2010063936A1 (pt)

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CN104634719A (zh) * 2014-12-29 2015-05-20 中国科学院长春光学精密机械与物理研究所 一种流式细胞仪的流动室
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Also Published As

Publication number Publication date
WO2010063936A9 (fr) 2010-08-19
RU2011127235A (ru) 2013-01-10
US20110235030A1 (en) 2011-09-29
EP2352984B1 (fr) 2019-05-22
US9243992B2 (en) 2016-01-26
CN102239400B (zh) 2017-10-10
WO2010063936A1 (fr) 2010-06-10
FR2939199B1 (fr) 2011-02-11
BRPI0921518A2 (pt) 2018-05-29
PL2352984T3 (pl) 2019-11-29
CN102239400A (zh) 2011-11-09
BRPI0921518B1 (pt) 2019-06-04
EP2352984A1 (fr) 2011-08-10
RU2511065C2 (ru) 2014-04-10
JP2012510627A (ja) 2012-05-10
MX2011005656A (es) 2011-06-17
FR2939199A1 (fr) 2010-06-04

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