BRPI0922411A2 - classificação de templates de ácido nucléico - Google Patents

classificação de templates de ácido nucléico

Info

Publication number
BRPI0922411A2
BRPI0922411A2 BRPI0922411A BRPI0922411A BRPI0922411A2 BR PI0922411 A2 BRPI0922411 A2 BR PI0922411A2 BR PI0922411 A BRPI0922411 A BR PI0922411A BR PI0922411 A BRPI0922411 A BR PI0922411A BR PI0922411 A2 BRPI0922411 A2 BR PI0922411A2
Authority
BR
Brazil
Prior art keywords
nucleic acid
acid template
template classification
classification
nucleic
Prior art date
Application number
BRPI0922411A
Other languages
English (en)
Inventor
Flusberg Benjamin
Hanes Jeremiah
Lee Jessica
Lyle John
Sorenson Jon
Korlach Jonas
Travers Kevin
Jia Lei
Turner Stephen
Original Assignee
Pacific Biosciences California Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Family has litigation
First worldwide family litigation filed litigation Critical https://patents.darts-ip.com/?family=42243263&utm_source=google_patent&utm_medium=platform_link&utm_campaign=public_patent_search&patent=BRPI0922411(A2) "Global patent litigation dataset” by Darts-ip is licensed under a Creative Commons Attribution 4.0 International License.
Application filed by Pacific Biosciences California Inc filed Critical Pacific Biosciences California Inc
Publication of BRPI0922411A2 publication Critical patent/BRPI0922411A2/pt

Links

Classifications

    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6869Methods for sequencing
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844Nucleic acid amplification reactions
    • C12Q1/6858Allele-specific amplification
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813Hybridisation assays
    • C12Q1/6834Enzymatic or biochemical coupling of nucleic acids to a solid phase
    • C12Q1/6837Enzymatic or biochemical coupling of nucleic acids to a solid phase using probe arrays or probe chips
    • CCHEMISTRY; METALLURGY
    • C12BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12QMEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2561/00Nucleic acid detection characterised by assay method
    • C12Q2561/113Real time assay

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Engineering & Computer Science (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Biophysics (AREA)
  • General Engineering & Computer Science (AREA)
  • Immunology (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Analytical Chemistry (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • Biotechnology (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
BRPI0922411A 2008-12-11 2009-12-10 classificação de templates de ácido nucléico BRPI0922411A2 (pt)

Applications Claiming Priority (4)

Application Number Priority Date Filing Date Title
US20155108P 2008-12-11 2008-12-11
US18035009P 2009-05-21 2009-05-21
US18666109P 2009-06-12 2009-06-12
PCT/US2009/006517 WO2010068289A2 (en) 2008-12-11 2009-12-10 Classification of nucleic acid templates

Publications (1)

Publication Number Publication Date
BRPI0922411A2 true BRPI0922411A2 (pt) 2018-06-05

Family

ID=42243263

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0922411A BRPI0922411A2 (pt) 2008-12-11 2009-12-10 classificação de templates de ácido nucléico

Country Status (7)

Country Link
US (3) US9175341B2 (pt)
EP (1) EP2370598B1 (pt)
CN (1) CN102317473A (pt)
AU (1) AU2009325069B2 (pt)
BR (1) BRPI0922411A2 (pt)
CA (1) CA2746632C (pt)
WO (1) WO2010068289A2 (pt)

Families Citing this family (101)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US8431367B2 (en) 2007-09-14 2013-04-30 Predictive Biosciences Corporation Detection of nucleic acids and proteins
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US12527665B2 (en) * 2008-12-11 2026-01-20 Pacific Biosciences Of California, Inc. Methods for identifying modified bases in nucleic acid templates
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
US9175338B2 (en) * 2008-12-11 2015-11-03 Pacific Biosciences Of California, Inc. Methods for identifying nucleic acid modifications
EP2391732B1 (en) 2009-01-30 2015-05-27 Oxford Nanopore Technologies Limited Methods using adaptors for nucleic acid constructs in transmembrane sequencing
WO2010117420A2 (en) 2009-03-30 2010-10-14 Pacific Biosciences Of California, Inc. Fret-labeled compounds and uses therefor
US8518643B2 (en) * 2010-02-04 2013-08-27 Pacific Biosciences Of California, Inc. Method to improve single molecule analyses
US9115353B2 (en) 2011-01-31 2015-08-25 Illumina, Inc. Method for nucleotide detection
EP2694686B2 (en) 2011-04-06 2023-07-19 The University of Chicago COMPOSITION AND METHODS RELATED TO MODIFICATION OF 5-METHYLCYTOSINE (5mC)
US10323236B2 (en) 2011-07-22 2019-06-18 President And Fellows Of Harvard College Evaluation and improvement of nuclease cleavage specificity
JP6298404B2 (ja) 2011-07-25 2018-03-20 オックスフォード ナノポール テクノロジーズ リミテッド 膜貫通ポアを用いる二重鎖ポリヌクレオチド配列決定のためのヘアピンループ方法
US9347900B2 (en) 2011-10-14 2016-05-24 Pacific Biosciences Of California, Inc. Real-time redox sequencing
EP3425065B1 (en) * 2011-12-13 2021-04-21 Oslo Universitetssykehus HF Methods and kits for detection of methylation status
US9238836B2 (en) * 2012-03-30 2016-01-19 Pacific Biosciences Of California, Inc. Methods and compositions for sequencing modified nucleic acids
US9062091B2 (en) 2012-02-15 2015-06-23 Pacific Biosciences Of California, Inc. Polymerase enzyme substrates with protein shield
JP6224689B2 (ja) * 2012-03-15 2017-11-01 ニユー・イングランド・バイオレイブス・インコーポレイテツド シトシンとこれの修飾物とを識別するための、およびメチローム分析のための方法および組成物
CA2867489A1 (en) * 2012-03-30 2013-10-03 Pacific Biosciences Of California, Inc. Methods and composition for sequencing modified nucleic acids
US9175348B2 (en) 2012-04-24 2015-11-03 Pacific Biosciences Of California, Inc. Identification of 5-methyl-C in nucleic acid templates
US20150094210A1 (en) * 2012-05-14 2015-04-02 Bgi Diagnosis Co., Ltd. Method, system and computer readable medium for determining base information in predetermined area of fetus genome
GB2559073A (en) * 2012-06-08 2018-07-25 Pacific Biosciences California Inc Modified base detection with nanopore sequencing
US11155860B2 (en) 2012-07-19 2021-10-26 Oxford Nanopore Technologies Ltd. SSB method
CN104955960A (zh) 2012-11-30 2015-09-30 剑桥表现遗传学有限公司 用于修饰的核苷酸的氧化剂
US9665694B2 (en) * 2013-01-31 2017-05-30 Codexis, Inc. Methods, systems, and software for identifying bio-molecules with interacting components
GB201314695D0 (en) 2013-08-16 2013-10-02 Oxford Nanopore Tech Ltd Method
CA2901545C (en) 2013-03-08 2019-10-08 Oxford Nanopore Technologies Limited Use of spacer elements in a nucleic acid to control movement of a helicase
CN105051208B (zh) * 2013-03-28 2017-04-19 深圳华大基因股份有限公司 确定胚胎基因组中预定区域碱基信息的方法、系统和计算机可读介质
US9957291B2 (en) 2013-08-05 2018-05-01 Pacific Biosciences Of California, Inc. Protected fluorescent reagent compounds
US9163284B2 (en) 2013-08-09 2015-10-20 President And Fellows Of Harvard College Methods for identifying a target site of a Cas9 nuclease
US9359599B2 (en) 2013-08-22 2016-06-07 President And Fellows Of Harvard College Engineered transcription activator-like effector (TALE) domains and uses thereof
EP3936222A1 (en) * 2013-08-30 2022-01-12 University of Washington through its Center for Commercialization Selective modification of polymer subunits to improve nanopore-based analysis
US9526784B2 (en) 2013-09-06 2016-12-27 President And Fellows Of Harvard College Delivery system for functional nucleases
US9228207B2 (en) 2013-09-06 2016-01-05 President And Fellows Of Harvard College Switchable gRNAs comprising aptamers
US9322037B2 (en) 2013-09-06 2016-04-26 President And Fellows Of Harvard College Cas9-FokI fusion proteins and uses thereof
CN106459995B (zh) 2013-11-07 2020-02-21 爱迪塔斯医药有限公司 使用统治型gRNA的CRISPR相关方法和组合物
US20150165054A1 (en) 2013-12-12 2015-06-18 President And Fellows Of Harvard College Methods for correcting caspase-9 point mutations
GB201403096D0 (en) 2014-02-21 2014-04-09 Oxford Nanopore Tech Ltd Sample preparation method
US10077453B2 (en) 2014-07-30 2018-09-18 President And Fellows Of Harvard College CAS9 proteins including ligand-dependent inteins
EP3633047B1 (en) 2014-08-19 2022-12-28 Pacific Biosciences of California, Inc. Method of sequencing nucleic acids based on an enrichment of nucleic acids
GB201418159D0 (en) 2014-10-14 2014-11-26 Oxford Nanopore Tech Ltd Method
KR101719285B1 (ko) * 2014-11-04 2017-03-23 한국과학기술원 표적 물질에 의해 조절되는 핵산 중합효소 활성을 이용한 생체물질의 검출 및 정량 방법
CA2971589C (en) 2014-12-18 2021-09-28 Edico Genome Corporation Chemically-sensitive field effect transistor
US9857328B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Chemically-sensitive field effect transistors, systems and methods for manufacturing and using the same
US10006910B2 (en) 2014-12-18 2018-06-26 Agilome, Inc. Chemically-sensitive field effect transistors, systems, and methods for manufacturing and using the same
US9618474B2 (en) 2014-12-18 2017-04-11 Edico Genome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US9859394B2 (en) 2014-12-18 2018-01-02 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10020300B2 (en) 2014-12-18 2018-07-10 Agilome, Inc. Graphene FET devices, systems, and methods of using the same for sequencing nucleic acids
US10302972B2 (en) 2015-01-23 2019-05-28 Pacific Biosciences Of California, Inc. Waveguide transmission
EP3258952A4 (en) 2015-02-04 2018-12-05 Pacific Biosciences Of California, Inc. Multimeric protected fluorescent reagents
CA2995422A1 (en) 2015-08-12 2017-02-16 The Chinese University Of Hong Kong Single-molecule sequencing of plasma dna
EP3355939A4 (en) 2015-09-30 2019-04-17 Trustees of Boston University MICROBIAL DEADMAN AND PASS CODE EMERGENCY STOP SWITCHES
IL310721B2 (en) 2015-10-23 2025-11-01 Harvard College Nucleobase editors and uses thereof
US10280411B2 (en) 2015-10-27 2019-05-07 Pacific Biosciences of California, In.c Methods, systems, and reagents for direct RNA sequencing
WO2017087696A1 (en) 2015-11-18 2017-05-26 Pacific Biosciences Of California, Inc. Methods and compositions for loading of polymerase complexes
CN108350487B (zh) 2015-11-19 2022-05-27 加利福尼亚太平洋生物科学股份有限公司 用于改善信号检测的化合物和系统
US10676788B2 (en) 2015-11-20 2020-06-09 Pacific Biosciences Of California, Inc. Modified nucleotide reagents
EP3376996B1 (en) 2015-11-20 2022-09-07 Pacific Biosciences of California, Inc. Protected dye-labeled reagents
CN113321943A (zh) 2015-11-20 2021-08-31 加利福尼亚太平洋生物科学股份有限公司 标记的核苷酸类似物、反应混合物以及测序方法和系统
US20170298422A1 (en) 2016-04-18 2017-10-19 The Board Of Trustees Of The Leland Stanford Junior University Simultaneous single-molecule epigenetic imaging of dna methylation and hydroxymethylation
WO2017201081A1 (en) 2016-05-16 2017-11-23 Agilome, Inc. Graphene fet devices, systems, and methods of using the same for sequencing nucleic acids
GB201609220D0 (en) 2016-05-25 2016-07-06 Oxford Nanopore Tech Ltd Method
CN110214183A (zh) 2016-08-03 2019-09-06 哈佛大学的校长及成员们 腺苷核碱基编辑器及其用途
WO2018029108A1 (en) 2016-08-08 2018-02-15 F. Hoffmann-La Roche Ag Basecalling for stochastic sequencing processes
WO2018031683A1 (en) 2016-08-09 2018-02-15 President And Fellows Of Harvard College Programmable cas9-recombinase fusion proteins and uses thereof
US11542509B2 (en) 2016-08-24 2023-01-03 President And Fellows Of Harvard College Incorporation of unnatural amino acids into proteins using base editing
CN107779485B (zh) * 2016-08-24 2022-03-18 中国科学院分子细胞科学卓越创新中心 Cmd1酶催化的5-甲基胞嘧啶核酸的新修饰及其应用
KR102622411B1 (ko) 2016-10-14 2024-01-10 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵염기 에디터의 aav 전달
WO2018119359A1 (en) 2016-12-23 2018-06-28 President And Fellows Of Harvard College Editing of ccr5 receptor gene to protect against hiv infection
CN117821565A (zh) * 2017-03-08 2024-04-05 芝加哥大学 高灵敏度dna甲基化分析方法
EP3592853A1 (en) 2017-03-09 2020-01-15 President and Fellows of Harvard College Suppression of pain by gene editing
US12390514B2 (en) 2017-03-09 2025-08-19 President And Fellows Of Harvard College Cancer vaccine
US11542496B2 (en) 2017-03-10 2023-01-03 President And Fellows Of Harvard College Cytosine to guanine base editor
KR20240116572A (ko) 2017-03-23 2024-07-29 프레지던트 앤드 펠로우즈 오브 하바드 칼리지 핵산 프로그램가능한 dna 결합 단백질을 포함하는 핵염기 편집제
LT3589748T (lt) * 2017-04-13 2020-10-26 Imba-Institut Für Molekulare Biotechnologie Gmbh Nukleorūgšties modifikavimo ir identifikavimo būdas
US11560566B2 (en) 2017-05-12 2023-01-24 President And Fellows Of Harvard College Aptazyme-embedded guide RNAs for use with CRISPR-Cas9 in genome editing and transcriptional activation
WO2019012481A2 (en) * 2017-07-12 2019-01-17 John Katz G-QUADRUPLEXES DIVIDED FOR CAPTURE AND DETECTION OF NUCLEIC ACIDS
US20230193359A1 (en) * 2017-07-27 2023-06-22 John Fred KATZ Split G-Quadruplexes for Capture and Detection of Nucleic Acids
CN111801345A (zh) 2017-07-28 2020-10-20 哈佛大学的校长及成员们 使用噬菌体辅助连续进化(pace)的进化碱基编辑器的方法和组合物
WO2019040546A1 (en) 2017-08-23 2019-02-28 Roche Sequencing Solutions, Inc ENZYME SCREENING METHODS
EP3676376B1 (en) 2017-08-30 2025-01-15 President and Fellows of Harvard College High efficiency base editors comprising gam
KR20250107288A (ko) 2017-10-16 2025-07-11 더 브로드 인스티튜트, 인코퍼레이티드 아데노신 염기 편집제의 용도
US12406749B2 (en) 2017-12-15 2025-09-02 The Broad Institute, Inc. Systems and methods for predicting repair outcomes in genetic engineering
CN108530539B (zh) * 2018-03-20 2021-08-13 北京博雅捷康生物科技有限公司 一组识别dna受紫外线照射造成的嘧啶二聚体的抗体
GB201807793D0 (en) 2018-05-14 2018-06-27 Oxford Nanopore Tech Ltd Method
US12157760B2 (en) 2018-05-23 2024-12-03 The Broad Institute, Inc. Base editors and uses thereof
WO2020006421A1 (en) 2018-06-29 2020-01-02 Pacific Biosciences Of California, Inc. Methods and compositions for delivery of molecules and complexes to reaction sites
US12522807B2 (en) 2018-07-09 2026-01-13 The Broad Institute, Inc. RNA programmable epigenetic RNA modifiers and uses thereof
WO2020092453A1 (en) 2018-10-29 2020-05-07 The Broad Institute, Inc. Nucleobase editors comprising geocas9 and uses thereof
CN111434778A (zh) * 2019-01-11 2020-07-21 中国人民解放军军事科学院军事医学研究院 一种RNA分子m6A修饰水平检测方法及用途
US12351837B2 (en) 2019-01-23 2025-07-08 The Broad Institute, Inc. Supernegatively charged proteins and uses thereof
WO2020191233A1 (en) 2019-03-19 2020-09-24 The Broad Institute, Inc. Methods and compositions for editing nucleotide sequences
US12473543B2 (en) 2019-04-17 2025-11-18 The Broad Institute, Inc. Adenine base editors with reduced off-target effects
SG11202101070QA (en) * 2019-08-16 2021-03-30 Univ Hong Kong Chinese Determination Of Base Modifications Of Nucleic Acids
US12435330B2 (en) 2019-10-10 2025-10-07 The Broad Institute, Inc. Methods and compositions for prime editing RNA
AU2021246531A1 (en) * 2020-04-02 2022-11-24 Altius Institute For Biomedical Sciences Methods, compositions, and kits for identifying regions of genomic DNA bound to a protein
CN115715321B (zh) * 2020-04-02 2026-03-10 阿尔泰斯生物医学科学研究所 用于鉴定基因组dna中与蛋白质结合的区域的方法、组合物和试剂盒
IL297761A (en) 2020-05-08 2022-12-01 Broad Inst Inc Methods and compositions for simultaneously editing two helices of a designated double-helix nucleotide sequence
EP4083231A1 (en) 2020-07-30 2022-11-02 Cambridge Epigenetix Limited Compositions and methods for nucleic acid analysis
US20250043336A1 (en) * 2023-08-04 2025-02-06 Pacific Biosciences Of California, Inc. Detection of 5-methylcytosine
WO2025193757A1 (en) 2024-03-12 2025-09-18 Pacific Biosciences Of California, Inc. Methods and systems for masking an array of biosensor elements

Family Cites Families (60)

* Cited by examiner, † Cited by third party
Publication number Priority date Publication date Assignee Title
US740528A (en) 1900-01-12 1903-10-06 Frederick W Braun Furnace.
US5547839A (en) 1989-06-07 1996-08-20 Affymax Technologies N.V. Sequencing of surface immobilized polymers utilizing microflourescence detection
WO1991006678A1 (en) 1989-10-26 1991-05-16 Sri International Dna sequencing
US5874239A (en) 1993-07-30 1999-02-23 Affymax Technologies N.V. Biotinylation of proteins
WO1996027025A1 (en) 1995-02-27 1996-09-06 Ely Michael Rabani Device, compounds, algorithms, and methods of molecular characterization and manipulation with molecular parallelism
ES2176358T3 (es) 1995-07-11 2002-12-01 Forfas Trading As Biores Irela Deteccion mediada por glucosilasa de secuencias nucleotidicas conocidas.
AUPO351996A0 (en) * 1996-11-08 1996-12-05 Western Health Care Network Viral variants and methods for detecting same
JP2001511358A (ja) 1997-07-28 2001-08-14 メディカル・バイオシステムズ・リミテッド 核酸配列の解析
US6787308B2 (en) 1998-07-30 2004-09-07 Solexa Ltd. Arrayed biomolecules and their use in sequencing
US6210896B1 (en) 1998-08-13 2001-04-03 Us Genomics Molecular motors
US6235502B1 (en) 1998-09-18 2001-05-22 Molecular Staging Inc. Methods for selectively isolating DNA using rolling circle amplification
WO2000036152A1 (en) 1998-12-14 2000-06-22 Li-Cor, Inc. A system and methods for nucleic acid sequencing of single molecules by polymerase synthesis
ATE556149T1 (de) 1999-02-23 2012-05-15 Caliper Life Sciences Inc Manipulation von mikropartikeln in mikrofluidischen systemen
US7056661B2 (en) 1999-05-19 2006-06-06 Cornell Research Foundation, Inc. Method for sequencing nucleic acid molecules
US7611869B2 (en) 2000-02-07 2009-11-03 Illumina, Inc. Multiplexed methylation detection methods
US6917726B2 (en) 2001-09-27 2005-07-12 Cornell Research Foundation, Inc. Zero-mode clad waveguides for performing spectroscopy with confined effective observation volumes
GB0016473D0 (en) 2000-07-05 2000-08-23 Amersham Pharm Biotech Uk Ltd Sequencing method
AU2001279134A1 (en) 2000-07-31 2002-02-13 Maxygen, Inc. Nucleotide incorporating enzymes
US7244566B2 (en) 2001-08-29 2007-07-17 Ge Healthcare Bio-Sciences Corp. Analyte detection
US7256019B2 (en) 2001-08-29 2007-08-14 Ge Healthcare Bio-Sciences Corp. Terminal phosphate blocked nucleoside polyphosphates
US7033762B2 (en) 2001-08-29 2006-04-25 Amersham Biosciences Corp Single nucleotide amplification and detection by polymerase
DE10151055B4 (de) * 2001-10-05 2005-05-25 Epigenomics Ag Verfahren zum Nachweis von Cytosin-Methylierung in CpG Inseln
US20030166170A1 (en) 2002-01-17 2003-09-04 Invitrogen Corporation Methods of random mutagenesis and methods of modifying nucleic acids using translesion DNA polymerases
EP1567669B1 (en) 2002-12-02 2010-03-24 Illumina Cambridge Limited Determination of methylation of nucleic acid sequences
EP1597602A4 (en) 2003-02-05 2009-07-22 Ge Healthcare Bio Sciences SOLID PHASE SEQUENCING
US20060024676A1 (en) 2003-04-14 2006-02-02 Karen Uhlmann Method of detecting epigenetic biomarkers by quantitative methyISNP analysis
US7670405B2 (en) 2003-08-06 2010-03-02 Department Of Science & Technology (Dst) Process for the manufacture of a bio-release fertilizer of an anionic micro nutrient viz molybdenum
JP2005204652A (ja) 2003-12-16 2005-08-04 Bayer Healthcare Llc メチル化特異的プライマー伸長(mspe)によるメチル化状況を検出するアッセイ
WO2005065321A2 (en) * 2003-12-29 2005-07-21 Nugen Technologies, Inc. Methods for analysis of nucleic acid methylation status and methods for fragmentation, labeling and immobilization of nucleic acids
WO2005085477A1 (en) 2004-03-02 2005-09-15 Orion Genomics Llc Differential enzymatic fragmentation by whole genome amplification
US20050239085A1 (en) 2004-04-23 2005-10-27 Buzby Philip R Methods for nucleic acid sequence determination
US7399614B2 (en) 2004-06-30 2008-07-15 Applera Corporation 5-methylcytosine detection, compositions and methods therefor
CA2579150C (en) * 2004-09-17 2014-11-25 Pacific Biosciences Of California, Inc. Apparatus and method for analysis of molecules
US7170050B2 (en) 2004-09-17 2007-01-30 Pacific Biosciences Of California, Inc. Apparatus and methods for optical analysis of molecules
US20070020640A1 (en) 2005-07-21 2007-01-25 Mccloskey Megan L Molecular encoding of nucleic acid templates for PCR and other forms of sequence analysis
US7805081B2 (en) 2005-08-11 2010-09-28 Pacific Biosciences Of California, Inc. Methods and systems for monitoring multiple optical signals from a single source
CA2631829C (en) 2005-12-14 2012-01-24 F. Hoffmann-La Roche Ag New method for bisulfite treatment
CA2633524A1 (en) 2005-12-22 2007-07-05 Pacific Biosciences Of California, Inc. Polymerases for nucleotide analogue incorporation
WO2008028160A2 (en) 2006-09-01 2008-03-06 Pacific Biosciences Of California, Inc. Substrates, systems and methods for analyzing materials
US20080277595A1 (en) 2007-05-10 2008-11-13 Pacific Biosciences Of California, Inc. Highly multiplexed confocal detection systems and methods of using same
CA2689626C (en) 2007-06-06 2016-10-25 Pacific Biosciences Of California, Inc. Methods and processes for calling bases in sequence by incorporation methods
US8486634B2 (en) 2007-11-06 2013-07-16 Ambergen, Inc. Amplifying bisulfite-treated template
US7968702B2 (en) 2008-03-13 2011-06-28 Pacific Biosciences Of California, Inc. Labeled reactants and their uses
US7973146B2 (en) 2008-03-26 2011-07-05 Pacific Biosciences Of California, Inc. Engineered fluorescent dye labeled nucleotide analogs for DNA sequencing
EP4230747A3 (en) 2008-03-28 2023-11-15 Pacific Biosciences Of California, Inc. Compositions and methods for nucleic acid sequencing
WO2009120374A2 (en) 2008-03-28 2009-10-01 Pacific Biosciences Of California, Inc. Methods and compositions for nucleic acid sample preparation
WO2009132315A1 (en) 2008-04-24 2009-10-29 Life Technologies Corporation Method of sequencing and mapping target nucleic acids
WO2010003153A2 (en) 2008-07-03 2010-01-07 Life Technologies Corporation Methylation analysis of mate pairs
US20100041057A1 (en) 2008-08-18 2010-02-18 Life Technologies Corporation Dna methylation detection methods
EP2331934B1 (en) 2008-09-16 2020-01-01 Pacific Biosciences of California, Inc. Analytic device including a zero mode waveguide substrate
US8383369B2 (en) 2008-09-24 2013-02-26 Pacific Biosciences Of California, Inc. Intermittent detection during analytical reactions
US9115386B2 (en) 2008-09-26 2015-08-25 Children's Medical Center Corporation Selective oxidation of 5-methylcytosine by TET-family proteins
WO2010048337A2 (en) 2008-10-22 2010-04-29 Illumina, Inc. Preservation of information related to genomic dna methylation
US8486630B2 (en) 2008-11-07 2013-07-16 Industrial Technology Research Institute Methods for accurate sequence data and modified base position determination
US8370079B2 (en) 2008-11-20 2013-02-05 Pacific Biosciences Of California, Inc. Algorithms for sequence determination
AU2009325069B2 (en) 2008-12-11 2015-03-19 Pacific Biosciences Of California, Inc. Classification of nucleic acid templates
WO2010111674A2 (en) 2009-03-27 2010-09-30 Life Technologies Corporation Methods and apparatus for single molecule sequencing using energy transfer detection
US9063156B2 (en) 2009-06-12 2015-06-23 Pacific Biosciences Of California, Inc. Real-time analytical methods and systems
US20110104787A1 (en) 2009-11-05 2011-05-05 President And Fellows Of Harvard College Fusion Peptides That Bind to and Modify Target Nucleic Acid Sequences
US20110237444A1 (en) 2009-11-20 2011-09-29 Life Technologies Corporation Methods of mapping genomic methylation patterns

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US20130316916A1 (en) 2013-11-28
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AU2009325069B2 (en) 2015-03-19
US20100221716A1 (en) 2010-09-02
US9175341B2 (en) 2015-11-03
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WO2010068289A2 (en) 2010-06-17
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EP2370598A4 (en) 2012-06-13
CA2746632C (en) 2020-06-30
WO2010068289A3 (en) 2010-10-14
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