BRPI1003373A2 - synergistic combinations of triazoles, strobirulins and benzimidazoles, uses, formulations, production processes and applications using the same - Google Patents

Abstract

SYNERGIC COMBINATIONS OF THREADS, STROBIRULINS AND BENZIMIDAZARES, USES, FORMULATIONS, PRODUCTION PROCESSES AND APPLICATIONS USING THE SAME. The present invention relates to a synergistic agrochemical formulation of triazoles, strobirulins and benzimidazoles, in specific proportions for the control and / or control of pests and diseases caused by them in plant crops. Their process of preparation, use and method of use as well as the use of triazoles, strobilurins and benzimidazoles in the preparation of the synergistic agrochemical formulation of the invention are further described.

Description

Patent Descriptive Report for "SYNERGIC COMBINATIONS OF TREIZOES, STROBIRULINS AND BENZYMIDAZOLE, USES, FORMULATIONS, PRODUCTION PROCESSES AND APPLICATIONS USING THE SAME". BACKGROUND OF THE INVENTION

The present invention relates to the balanced synergistic combination of active ingredients for disease control in soybean, cotton, corn, beans, wheat, rice, potatoes, tomatoes, citrus and coffee.

Tebuconazole is a fungicide known to those skilled in the art for controlling various plant diseases. EP 40 345 provides a description of tebuconazole and some uses. It is a fungicide of the triazole chemical group and acts by inhibiting the biosynthesis of ergosterol, an important substance for maintaining the integrity of the fungal cell membrane. Tebuconazole is generally known as (RS) -Ip-chlorophenyl-4,4-dimethyl-3- (1H-1,2,4-triazol-1-ylmethyl) pentan-3-ol and has the registration number CAS 107534 -96-3. Tebuconazole is described in The Pesticide Manual, page 1072 (entry 808), C.D.S. Thomas, Ed (15th Ed., 2009).

Flutriafol is a fungicide known to those skilled in the art for controlling various plant diseases. EP 15 756 provides a description of flutriafol and some uses. It is a fungicide of the triazole chemical group and acts by inhibiting the biosynthesis of ergosterol, an important substance for maintaining the integrity of the fungal cell membrane. Flutriol is generally known as (RS) -2,4'-difluoro-a- (1H-1,2,4-triazol-1-ylmethyl) benzhydryl alcohol and has the registration number CAS 76674-21-0 . Flutriafol is described in The Pesticide Manual, page 560 (entry 422), C.D.S. Thomas, Ed (15th Ed., 2009).

Carbendazim is a fungicide known to those skilled in the art to control various plant diseases. A more detailed description with some uses can be seen in US Patent 3,010,968. It is a fungicide from the chemical group of benzimidazoles and acts specifically on cell division by inhibiting tubulin biosynthesis, which is a protein that makes up microtubules. Thus, the formation of microtubules is distorted without the division of the nucleus and the consequent separation. Carbendazim is generally known as 2-methoxybenzimidazol-2-yl) carbamic acid and has the registration number CAS 10605-21-7. Carbendazim is described in The Pesticide Manual, page 158 (entry 123), C.D.S. Thomas, Ed (15th Ed., 2009).

Azoxystrobin is a fungicide known to those skilled in the art to control various plant diseases. A more detailed description with some uses can be seen in patent EP 382 375. It is a fungicide of the chemical group strobirulins and acts by inhibiting myotondrial respiration, which blocks the transfer of electrons between the cytochrome b and cytochrome c1 at the site. Qo1 interfering with ATP production. Azoxystrobin is commonly known as methyl (E) -2- [2- [6- (2-cyanophenoxy) pyrimidin-4-yl] oxyphenyl] -3-ethoxyprop-2-enoate and has the registration number CAS 215934- 32-0. Azoxystrobin is described in The Pesticide Manual, page 62 (entry 52), C.D.S. Thomas, Ed (15th Ed., 2009).

Kresoxim-methyl is a fungicide known to those skilled in the art to control various plant diseases. A more detailed description with some uses can be seen in EP 253 213. It is a fungicide of the chemical group strobirulins and acts by inhibiting mitochondrial respiration, which blocks electron transfer between the cytochrome b and cytochrome c1, interfering with the Qo1 site. in the production of ATP. Kresoxim-methyl is generally known as methyl (E) -methoxyimino [a- (o-tolyloxy) -o-tolyljacetate and has the registration number CAS 143390-89-0. Kresoxim-methyl is described in The Pesticide Manual, page 688 (entry 517), C.D.S. Thomas, Ed (15th Ed., 2009).

The present invention may be practiced to control the following diseases in the following cultures:

Soybean cropping: Custospora kerchis leaf crest; American Rust (Phakopsora meibomiae); Asian rust (Phakopsora pachyrhizi); Alternaria leaf spot (Alternaria sp); Brown spot (Septoria glycines); Spotted frog (Cercospora sojina); Target spot (Corynespora cassiicola); Soybean molasses or late blight (Rhizoctonia solani); Mildew (Erysiphe diffusa); Anthracnose (Colletotrichum dematium var. Truncata); White stem rot (Sclerotinia sclerotiorum).

Cotton culture: Ramulose (Colletotrichum gossypii var. Cephalosporioides); Ramularia (Ramularia areola); Alternaria Spot (Alternaria sp); Mirothecium (Myrothecium roridum); Rust (Phakopsora gossypii) (Puccinia cacabata).

Corn crop: Helminthosporiosis (Exerohilum turcicum); leaf blotch or Phospheria (Phaeosphaeria maydis); diploma spot (Diplodia macrospora); cercosporiosis (Cercospora zea-maydis); Anthracose (Colletotrichum graminicola); polishing rust (Puccinia polysora); tropical rust (physopella zeae); Common Rust (Puccinia sorghi)

Bean culture: angular spot (Phaeoisariopsis griseola); anthracnose (Colletotrichum lindemuthianum); white mold (Sclerotinia sclerotorum); powdery mildew (Erysiphe polygoni); rust (Uromyces appendiculatus). Wheat crop: stem rust (Puccinia graminis); spot

yellow (Drechslera tritici-repentis); Helminthosporiosis (Bipolaris sorokinia); leaf rust (Puccinia triticina); blast (Pyricularia grisea); powdery mildew (Blumeria graminis); septoriosis (Septoria tritici).

Rice culture: blast (Pyricularia grisea); brown spot (Bipolaris oryzae)

Potato culture: Alternaria spot (Alternaria solani) Tomato culture: Alternaria spot (Alternaria solani); sepsis (Septoria lycopersici)

Citrus culture: anthracnose (Colletotrichum gloeosporioides); alternaria spot (Alternaria citri); verugose (Elsinoe fawcetti)

Coffee culture: Rust (Hemileia vastatrix); cercosporiosis (Cercospora coffeicola); anthracnose (Colletotrichum coffeanum); ascochyta (Ascochyta coffeae); dry hands (Phoma spp.)

Plant diseases cause considerable losses in agricultural crops, both by reducing production capacity as well as by causing direct damage by reducing the commercial value of fruits, grains or tubers. It is reported in the literature by researchers that studying diseases in the main tropical crops, relates the percentages of crop losses due to diseases: sugar cane, 20-30%; banana, 20-29%; wheat, 10-12%; orange, 28-34%; maize 20-23%; beans, 20-35%; sorghum, 25-35%; rice, 35-50%; smoking, 17-25%; potato, 20-30%; tomato, 35-50%; paste, 17-30%; coffee, 20-27%; cotton, 15-70%; soybean 10-85% and cocoa 27-35%. The adoption of management practices is a very important component in the production system. Within the management tools, the use of fungicides is necessary given the need to reduce the pathogen population, as well as inhibit its development. Among the more than 250 cotton pathogens recorded in the

specialized literature, Cia and Fuzatto (1999) cite approximately 30 of them occurring in Brazil. Of these, except for seedlings and other pathogens of very recent occurrence, approximately 10 of them could be considered of primary importance for cotton in the country. The regional importance of pathogens varies considerably.

In general, traditional cultivation regions have higher numbers, while new regions start from a limited number of diseases, gradually incorporating new problems over the years. The spread and regional evolution of diseases result mainly from attitudes and care practiced regionally. A complex of factors governs pathogen introduction and dynamics in a given environment: proximity and exchange with other producing regions (countries), regional physiographic characteristics, prophylaxis, cultural measures, management systems adopted, rate of seed utilization and degree of resistance of cvs. used are the main ones.

The main leaf diseases of cotton culture in Brazil are: Ramulose (Colletotrichum gossypii var. Cephalosporioides); Ramularia (Ramularia areola Atk.); Alternaria Spot (Alternaria sp); Mirothecium (Myrothecium roridum); Rust (Phakopsora gossypii) (Puccinia cacabata). Corn (Zea mays L.) is also one of the most widely cultivated

importance in Brazilian agriculture. Its yield can be influenced by factors such as water availability, soil fertility, plant population, cropping system, hybrid production potential and weed management, pests and diseases.

In Brazil, many diseases are reported in maize, and recently the most frequent ones are related to stalk and ear rot and fungal leaf diseases. In the high-tech production system, leaf diseases have been given greater importance, since materials with higher production potential have shown greater sensitivity to these diseases. Thus, the management with the use of foliar fungicides is providing gains in productivity in this crop.

Stalk rot is the rotting of stalk tissues evident when plants are near harvest. The damage caused is attributed to the interruption of the normal grain filling process, plant lodging and premature plant death at the end of the crop cycle. In Brazil, the fungi Colletotrichum graminicola (Ces) G.W. Wils., Stenocarpella maydis (Berk.) Sutton [Sin. Diplodia maydis (Berk.) Sacc., S. macrospora (EarIe) Sutton [Sin. D. macrospora Earle in Bull.], Fossarium graminearum Schwabe (Gibberella zeae Schw.) And Fusarium verticil-Iioides [Sin. = Fusarium moniliform J. Sheld (Gibberella fujikuroi Sawada)] are cited as the main causal agents of stem rot. (Pereira, 1997; Pintoetal., 1997).

With the exception of C. graminicola, the other aforementioned fungi commonly cause ear rot (Pinto et al., 1997), and are often isolated from maize seeds (Casa et al., 1998; Pinto, 1998) . In the field, infected grains may exhibit so-called discoloration symptoms of burnt grains. Thus, rotten cobs reduce grain yield and grain quality, since in marketing it is discounted from the offered price a percentage referring to the incidence of burnt grain. Also in the case of spike rot, damage quantification data are scarce and inaccurate.

The main leaf spots are common helminths, caused by the fungus Exerohilum turcicum (Pass.) Leonar & Suggs., The white leaf spot or phospheria, caused by Phaeosphaeria maydis (P. Henn.) Rae1 Payak & Renfro1 and the diplodia spot. , caused by S. macrospora. Currently, in Brazil, it is worth mentioning the cercosporiosis caused by the fungus Cercospora zea-maydis Tenhon & Daniels, first discovered in the country by Viégas and Krug, in 1934, in Campinas, São Paulo (Viégas, 1945). Since then, the disease has practically ceased to be observed in our country or there has been no report of its epidemic occurrence. However, in the 2000 corn crop in southwestern Goiás, the disease resurfaced devastatingly, destroying the entire healthy leaf area of the plants and, in a short period of time, eventually killing the plants early. Other important diseases in maize crop that are causing losses in commercial areas are: anthracnose (Colletotrichum graminicola); polishing rust (Puccinia polysora); tropical rust (Physopella zeae); common rust (Puccinia sorghi). Diseases are the main factors limiting the attainment of

high soybean yields. Approximately 40 diseases caused by fungi, bacteria, nematodes and viruses have been identified in Brazil. This number continues to increase as soybeans expand into new areas and as a result of monoculture. The economic importance of each disease varies from year to year and from region to region, depending on the climatic conditions of each crop. Annual production losses due to diseases are estimated at around 15% to 20%, however, some diseases may cause losses of almost 100%.

The following soybean diseases have been identified in Brazil. Its occurrences may range from sporadic or restricted to nationally generalized incidence. Leaf Diseases: Leaf blight of cercospora (Cercospora kikuchii); American Rust (Phakopsora meibomiae); Asian rust (Phakopsora pachyrhizi); Alternaria leaf spot (Alternaria sp); Ascochyta leaf spot (Ascochyta soye); Leaf spot of Mythothium (Myrothecium roridum); Brown spot (Septoria glycines); Spotted frog (Cercospora sojina); Mildew (Peronospora manshurica); Phyllosticta (Phyllosticta sojicola) spot; Target spot (Corynespora cassiicola); Soybean molasses or late blight (Rhizoctonia solani); Mildew (Erysiphe diffusa). Stem, pod and seed diseases: Anthracnose (Colletotrichum dematium var. Truncata); Stem cancer (Diaporthe phaseolorum f.sp. meridionalis); Purple spot (Cercospora kikuchii); Stem and pod drought (Phomopsis spp.); Pod drought (Fusarium spp.); Yeast Stain (Nematospore corily); White stem rot (Sclerotinia sclerotiorum), in addition to other root diseases, bacterial diseases, diseases caused by viruses, diseases caused by nematodes and diseases of undefined cause.

Among all disease complexes in soybean crop, Asian rust deserves special attention due to its high virulence, potential for infection, area of occurrence, aggressiveness and high speed of dissemination. Asian rust, caused by the fungus Phakopsora pachyrhizi Sydow, has caused significant damage to soybean crops. According to Caldwell & Laing (2005), the inoculum reached the African continent carried by air currents. In South America it was first described in Paraguay by Morei (2001), followed by Brazil, Yorinori (2002), Uruguay, Argentina and Bolivia. Yield damage has ranged from 10 to 85%, especially in areas where control is not performed or is late.

The symptoms are particularly evident on the leaves, evolving

from isolated uredias to areas with pronounced coalescence when it causes yellowing and premature leaf abscission. They are grouped into tan-colored lesions with two to five uredias and abundant sporulation (B) or forming brownish-red lesions with zero to two uredias and sparse sputum (PA) (Bromfield, 1984).

Prolonged leaf wetness (10 h / day), nocturnal temperature between 18 and 24 ° C, and frequent rainfall are determinant conditions for the establishment of the disease. Disease spread has occurred at a rate of more than 1 m / day (Southern Region Soybean Research Meeting, 2002.). According to Bromfield (1984), early flowering infections produce high levels of damage, also affecting grain protein content (Ogle et al., 1979). Preventively applied fungicides have stood out as the most effective strategy for controlling this disease (Azevedo, 2001; Hartman et al., 1991). Longer residual period and better fungicide performance were obtained by Vitti et al. (2004), due to the preventive application of fungicides. Similarly, Oliveira (2004) observed an increase in income of up to 100% when preventively controlling the disease.

Until the 2005/06 crop, the fungus was efficiently controlled by the use of fungicides, especially triazoles alone or in admixture with strobilurins (Silva et al., 2006). However, from the 2005/06 harvest, control failures were found in some regions of Mato Grosso, which were treated with triazole flutriafol. In this season, no problems were found in Goiás (Silva et al., 2008a).

From the 2006/07 harvest, the problems of triazole efficacy, even in preventive applications, were verified in experiments conducted by Silva et al. (2008b). For these authors, the problems were found not only for flutriafol, but also for the other triazoles, such as tebuconazole and cyproconazole.

In the 2008/09 crop, problems were also found for mixtures (strobilurins + triazoles) when curatively used under high pressure (unpublished data). This fact was expected since they have triazoles in their composition and the benefit of strobilurin is known to be preventive.

In the 2007/2008 harvest, tetabonazole was less efficient in trials conducted in late sowing (Godoy, 2009). This lower efficiency has been associated with the selection of less sensitive triazole fungal populations during the season, as demonstrated by the fungal sensitivity monitoring carried out since 2005 by Bayer Cropsciences (Mehl, 2009).

There are several fungicide classification criteria. Classification is generally based on the chemical nature and form of action of the product. The classification based on mode of action is as follows: contact action protectors; contact with eradicating action; with systemic and eradicating action; deep action penetrating and resistance inducers. As for resistance inducers, there is still little evidence.

Protective fungicides prevent the successful penetration of the fungus into host tissue. Of the fungicides available in soybean rust management, the strobilurin fungicide class (azoxystrobin, piraclostrobin, trifloxystrobin, etc.) has the ability to disrupt spore germination and host penetration. However, it has little or no effect once the fungus has penetrated or colonized the host plant tissue.

Curative fungicides have the ability to inhibit or interrupt

per the development of infections you have already started. Some fungicides have activity inhibiting sporulation which helps to retard disease development, limiting the reproductive potential of the fungus. Of the fungicides available in soybean rust management, only triazoles have a certain curative activity. It is this triazole activity that provides limited healing effect under conditions of low field rust levels. If one of the strobilurin fungicides is applied after infection, they will continue to develop. It is very important to remember that triazoles have limited healing activity. This is the main reason why fungicides are ineffective in controlling soybean rust under conditions of high or moderate field disease pressure.

The mobility of fungicides is differentiated. The main point is that different fungicides, even those in the same chemical class, are not necessarily equal when it comes to plant mobility. Systemic fungicides such as triazoles, benzimidazoles and strobilurins are absorbed by plants and redistributed to tissues at different mobility levels.

All strobilurin fungicides are absorbed and have translaminar translocation, but differences in systemic circulation have been observed between different strobirulins. For example, piraclostrobin is a strobilurin that has less mobility than azoxistrobin, which is absorbed by the plant but does not move much beyond the absorption point. Regardless of strobilurin, the leaves produced after application are not adequately protected. Triazole fungicides have higher systemic activity and, as a group, tend to be absorbed more rapidly and redistributed within the leaf and upwards (via xylem) to new developing leaves.

One of the main concerns in the association of fungicides is to reap the full benefits of the molecules used, such as the mode of action of each molecule, differentiated systematics and phases in which the fungicide provides pathogen control. Thus, the association of fungicides that have different modes of action and at different stages of the pathogen has better potential to control plant diseases, which can still be reflected in a greater residual control effect.

One of the concerns associated with strobirulin group fungicides and to a lesser extent for triazoles is the potential to develop resistance in populations of fungi such as Phakopsora pachyrhizi exposed to these fungicides. Resistance to strobirulin fungicides is already reported in several pathogens, as the mode of action is very specific resistance occurrence is facilitated, similarly for benzimidazoles. For triazoles, there are also several reports of resistant pathogens. However, the potential for resistance to appear is lower than for strobirulins.

The association of fungicide molecules with distinct mode of action is an important tool in the management of plant diseases as well as control and residual efficacy as well as the sustainability of control tools, as it imposes difficulties in the emergence of resistance, besides the fact that possibility of causing synergistic effect on interactions. SUMMARY OF THE INVENTION

The present invention relates to a synergistic combination of triazoles, strobirulins and benzimidazoles.

In particular, it is an object of the invention to provide synergistic combinations of triazole, strobirulin and benzimidazole fungicide molecules for the control of plant leaf diseases, the fungicides being referred to in different modes of action. In a particular feature of the invention said combinations comprise: tebuconazole + carbendazim + azoxystrobin; tebuconazole + carbendazim + kresoxim-methyl; flutriafol + carbendazim + azoxystrobin; flutriafol + carbendazim + kresoxim-methyl.

Another object of the invention is to provide a product with the

three fungicides, the combination of fungicide molecules meeting the following ratios: tebuconazole (25 to 250g / L); carbendazin (50 to 500g / l); azoxystrobin (5 to 200g / L), and the possibility of interaction of fungicide molecules meeting these relationships results in a product with high efficacy in controlling diseases in plants.

Another object of the invention is to provide a balanced and synergistic specific combination (premix) at the concentration of tebuconazole (100g / Lia, ia = active ingredient) + carbendazim (200g / Lia) + azoxystrobin (30g / Lia), coded as "BF 488", to control a broad spectrum of diseases in soybean, cotton, maize, bean, wheat, rice, potato, tomato, citrus and coffee crops.

Another object of the invention is to reduce the amount of active ingredient per hectare because the synergism of the molecules of the present invention BF 488 (tebuconazole + carbendazim + azoxystrobin) provides for the application of the lowest amount of active substance per ha of carbendazim and azoxystrobin.

Another important factor of the invention is the increased residual effect of the fungicides of the BF 488 product when compared to applied tebuconazole alone or applied azoxystrobin alone, this prolonged effect results in greater efficacy in disease control. Another important factor of the invention is increased spectrum of

disease control in crops with the application of product BF 488 (tebuconazole + carbendazim + azoxystrobin), with no need for product mixing in the spray tank as well as immediate re-entry into the area with another product. This is due to the synergistic association of three modes of action of fungicides, triazoles - which have high efficacy in controlling Ascomycetes and Basidiomycetes and a certain activity in Deuteromycetes; benzimidazoles - which have high efficacy in controlling Deuteromycetes and some activity in controlling Ascomycetes and Basidiomycetes; and strobirulins - which are highly effective in Deuteromycetes and have some activity in Oomycetes and Basidiomycetes and little activity in Ascomycetes.

Another important factor of the invention concerning the product BF 488 (tebuconazole + carbendazim + azoxystrobin) will be an important tool in the management of disease resistance to fungicides, as it is a mixture of three actives of different chemical groups and modes of action.

Another object of the invention is to provide a three fungicidal product encoded as "BF 452", the combination of fungicide molecules meeting the following ratios: tebuconazole (25 to 250g / L); carbendazin (50 to 500g / l); kresoxim-methyl (50 to 500g / L), with the possibility of fungicide molecules interacting in these relationships, which results in a product with high efficacy in controlling diseases in plants.

The specific combination of BF 452 is balanced and synergistic (premix), particularly with the concentration of tebuconazole (100g / Lia) + carbendazim (200g / Lia) + kresoxim-methyl (125g / Lia) to control a broad spectrum of diseases in soybean, cotton, corn, beans, wheat, rice, potatoes, tomatoes, citrus and coffee crops.

Another object of the invention is to reduce the amount of active ingredient per hectare, since the synergism of the molecules of the invention BF 452 (tebuconazole + carbendazim + kresoxim-methyl) provides for the application of the lowest amount of active ingredient per ha of carbendazim and kresoxim-methyl. .

Another important factor of the invention is the increased fungicide effect of the BF 452 product when compared to applied tebuconazole alone or applied kresoxim-methyl alone, this prolonged effect results in greater efficacy in disease control.

Another important factor of the invention is the increase of the disease control spectrum in the crops with the application of the product BF 452 (tebuconazole + carbendazim + kresoxim-methyl), with no need for mixing of products in the spray tank, as well as immediate re-entry into the area with another product. This is due to the synergistic association of three modes of action of fungicides, triazoles - which have high efficacy in controlling Ascomycetes and Basidiomycetes and a certain activity in Deuteromycetes; benzimidazoles - which have high efficacy in controlling Deuteromycetes and some activity in controlling Ascomycetes and Basidiomycetes; and strobirulins - which are highly effective in Deuteromycetes and have some activity in Oomycetes and Basidiomycetes and little activity in Ascomycetes.

Another important factor of the invention regarding the product BF 452 (tebuconazole + carbendazim + kresoxim-methyl) is an important tool in the management of disease resistance to fungicides as it is a mixture of three actives of different chemical groups and modes of action.

Another object of the invention is to provide a three fungicidal product encoded as "BF 489", the combination of fungicide molecules meeting the following ratios: flutriafol (25 to 250g / L); carbendazin (50 to 500g / l); azoxystrobin (5 to 200g / L), and the possibility of interaction of fungicide molecules meeting these relationships results in a product with high efficacy in controlling diseases in plants.

The specific combination of BF 489 is balanced and synergistic (premix), particularly showing the concentration of flutriafol (63g / Lia) + carbendazim (200g / Lia) + azoxystrobin (30g / Lia) to control a broad spectrum of diseases in soybean, cotton, corn, beans, wheat, rice, potato, tomato, citrus and coffee crops.

Another object of the invention is to reduce the amount of active ingredient per hectare since the synergism of the molecules of the invention BF 489 (fluoriafol + carbendazim + azoxystrobin) provides less active application per ha of carbendazim and azoxystrobin.

Another important factor of the invention is the increased residual effect of the BF 489 fungicides when compared with applied applied flutriafol alone or with applied applied azoxystrobin alone, this prolonged effect will result in greater efficacy in disease control. Another important factor of the invention is the increased spectrum of disease control in crops with the application of product BF 489 (fluctol + carbendazim + azoxystrobin), with no need for product mixing in the spray tank as well as re-entry. in the area with another product. This is due to the synergistic association of three fungicidal modes of action, triazoles - which have high efficacy in controlling Ascomycides and Basidiomycetes and certain activity in Deuteromycetes; benzimidazoles - which have high efficacy in controlling Deuteromycetes and some activity in controlling Ascomycetes and Basidiomycetes; and strobirulins - which are highly effective in Deuteromycetes and have some activity in Oomycetes and Basidiomycetes and little activity in Ascomycetes.

Another important factor of the invention regarding the BF 489 product (flutriafol + carbendazim + azoxystrobin) is the important tool in the management of disease resistance to fungicides as it is a mixture of three actives of different chemical groups and modes of action.

Another object of the invention is to provide a three fungicidal product encoded as "BF 465", the combination of fungicide molecules meeting the following ratios: flutriafol (25 to 250g / L); carbendazin (50 to 500g / l); kresoxim-methyl (50 to 500g / L), and the possibility of fungicide molecules interacting with these relationships results in a product with high efficacy in controlling diseases in plants.

The specific combination of BF 465 is balanced and synergistic (premix), particularly showing the concentration of flutriafol (63g / Lia) + carbendazim (200g / Lia) + kresoximethyl (125g / Lia) to control a broad spectrum of diseases in soybean, cotton, corn, beans, wheat, rice, potatoes, tomatoes, citrus and coffee crops.

Another object of the invention is to reduce the amount of active ingredient per hectare, since the synergism of the molecules of the invention BF 465 (fluoriafol + carbendazim + kresoxim-methyl) provides for the application of less active amount per ha of carbendazim and kresoxim-methyl. . Another important factor of the invention is the increased residual effect of the fungicides of product BF 465, when compared with applied applied flutriafol alone or with applied applied kresoxim-methyl alone, this prolonged effect will result in greater efficacy in disease control.

Another important factor of the invention is the increased spectrum of

disease control in crops with the application of product BF 465 (flutrial + carbendazim + kresoxim-methyl), with no need for mixing of products in the spray tank, as well as immediate re-entry into the area with another product. This is due to the synergistic association of three fungicidal modes of action, triazoles - which have high efficacy in controlling Ascomycetes and Basidiomycetes and a certain activity in Deuteromycetes; benzimidazoles - which have high efficacy in controlling Deuteromycetes and some activity in controlling Ascomycetes and Basidiomycetes; and strobirulins - which are highly effective in Deuteromycetes and have some activity in Oomycetes and Basidiomycetes and little activity in Ascomycetes.

Another important factor of the invention regarding the product BF 465 (flutriafol + carbendazim + kresoxim-methyl) is the important tool in the management of disease resistance to fungicides as it is a mixture of three actives of different chemical groups and modes of action. . DETAILED DESCRIPTION OF THE INVENTION

The present invention is a solid or liquid formulation, in suitable solid form in wettable powder (PM) and / or water dispersible granules (WDG) and in suitable liquid form as emulsifiable concentrate (EC). , concentrated suspension (SC), suspoemulsion (SE) and / or concentrated emulsion (EW), preferably as SC, not excluding other types of formulations.

The formulations deal with the mixture of the chemical groups benzimidazole, triazole and strobirulin. Assets and their proportions are shown below.

For the product BF488, the ratio of the amount of Carbendazim, Tebuconazole and Azoxystrobin is from 10 to 30 parts of Carbendazim, 5 to 15 parts of Tebuconazole and 1 to 5 parts of Azoxistrobin1 and, in a particularity of the invention, the ideal ratio is from 20: 10: 3, respectively.

For the product BF489, the ratio of the amount of Carbendazim, Flutriafol and Azoxystrobin is from 10 to 30 parts Carbendazim, 4 to 8 parts Flutriafol and 1 to 5 parts Azoxistrobin, and in one particular of the invention the Ideal ratio is 20: 6.25: 3 respectively.

For the product BF452, the ratio of the amount of Carbendazim, Tebuconazole and Krezoxim-Methyl is from 10 to 30 parts of Carbendazim, 5 to 15 parts of Tebuconazole and 6 to 18 parts of Krezoxim-Methyl. , the ideal ratio is 20: 10: 12.5, respectively.

For the product BF465, the ratio of Carbendazim, Flutriafoi and Krezoxim-Metil amounts to 10 to 30 parts Carbendazim, 4 to 8 parts Flutriafol and 6 to 18 parts Krezoxim-Metil. of the invention, the ideal ratio is 20: 6.55: 12.5, respectively.

The sum of the active ingredients in the final formulation may vary, usually from about 10 g / l to about 700 g / l (gia = gram of active ingredient). The remainder of the composition, up to 1 (one) liter, if liquid, or 1 (one) kilogram, if solid, may be comprised of suitable carrier and / or excipients, which may be selected from the group consisting of surfactants, defoamers. rheology modifying agents, dispersants, wetting agents, bactericides or bacteriostatic agents, anti-caking agents, stabilizing agents, antifreeze agents, among others, in addition to a mixture thereof.

The carrier should normally be inert, of organic or inorganic material and of natural or synthetic source. The vehicle, if solid, must be clay, silicates, silicas, resins, waxes, cellulosic fibers, fertilizers or similar, and may be used alone or in combination. If liquid, the vehicle shall be water, C1 to C14 chain alcohols, glycols, acetates, ketones, glycerides, saturated or unsaturated hydrocarbons, fatty acid esters, vegetable oils, mineral oils or the like. be used alone or in combination. Preferably use water as a vehicle.

The surfactants can be ionic, nonionic, cationic or polymeric.

Anionic surfactants for use in the present invention may

be: Dodecylbenzenic acid sulfonate, linear or branched, in acid or neutralized form, nitrogenous derivatives, sulfonated aromatic polymers in acid or neutralized form, acid or neutralized sulphonated formaldehydes, condensed or not, lignosulfonated, phosphate alkylates in acid or neutralized form. Preferably of these are ethoxylated or non-ethoxylated phosphate phosphates, and lignosulfonates.

The nonionic surfactants for use in the present invention may be: Alkoxylated, such as alkoxylated sorbitan esters, alkoxylated alcohols, alkoxylated vegetable oils, alkoxylated fatty acids, among others; fatty esters, such as polyethylene glycol esters, glycerol esters or polyglycerol esters sorbitan esters; amides, such as ethanolamine grease amide or ethylene amines, fatty imidazolines, among others. Preferably, caprylic, caprylic, isodecyl, isotridecyl, decyl, lauric, stearyl, oleic, nonylphenyl alkoxylated alcohols of 1 to 16 mo of ethylene oxide and / or propene oxide, alkoxy vegetable oils are used. such as alkoxylated castor oil with an alkoxylation degree of 5 to 54 mo of ethylene oxide or propene oxide, alkoxylated sorbitan esters with an alkoxylation degree of between 5 and 80 mo of ethylene oxide and / or propene oxide.

The cationic surfactants that may be used in the present invention are alkyl trimethyl ammonium hydrochloride, dialkyl dimethyl ammonium hydrochloride, alkyl hydroxyethyl dimethyl ammonium hydrochloride, cetyl trimethyl ammonium hydrochloride, distearyl dimethyl ammonium hydrochloride, quarten esters, among others.

The polymeric surfactants that may be used in the present invention are ethylene oxide and propene oxide block copolymers, mainly butyl chain copolymers, polyacrylate copolymers, methyl methacrylate copolymers, among others.

The present invention further relates to a process for preparing the formulations of the invention, for example the Concentrated Suspension formulation which comprises the steps of:

(i) in a container, mix water, surfactants and additives;

(ii) to this mixture is added, under stirring, the actives whose chemical groups are Benzimidazole, Triazole and Strobirulin;

(iii) After complete addition of the raw materials, the formulation is dispersed and undergoes a milling process, where there will be greater interaction between the molecules and the particle size reduction of the active ingredients, thus increasing the synergism between the molecules and their effectiveness in the field; and

(iv) after milling, the formulation still undergoes a deaeration and thickening process to increase its physical stability, thus obtaining a stable and synergistic agrochemical formulation.

The synergistic agrochemical formulations obtained above are then packaged or packaged in packages commonly used in the art.

It is yet another embodiment of the invention the use of benzimidazole, triazole and strobirulin in the preparation of formulations for the control and / or control of pests and diseases in plant crops.

The invention further comprises the use of the inventive formulations for controlling and / or controlling pests and diseases in plant crops.

Still another embodiment of the invention is a method of controlling and / or controlling pests and diseases in plant crops, comprising applying the formulations of the invention to said pests, their habitats and / or plant crops.

As mentioned above, the invention has broad and effective applicability in soybean, cotton, corn, bean, wheat, rice, potato, tomato, citrus and coffee crops, among others, in the control and control of Alternaria citri pests. , Alternaria solani, Alternaria sp, Ascochyta coffeae, Ascochyta soye, Bipolaris oryzae, Bipolaris sorokiniana, Blumeria graminis, Cercospora eoffeieola, Cercospora kikuehii, Cereospora sojina, Cereospora zea-maydis, Colletotumum and Collumotrichum e. truncata, Colletotrichum gloeosporioides, Colletotrichum gossypii var.

Cephalosporioides, Colletotrichum graminicola, Colletotrichum Iindemuthia- um, Corynespora cassiicola, Diaporthe phaseolorum f.sp. meridionalis, Dipplodia macrospora, Dreehslera tritici-repentis, Elsinoe fawcetti, Erysiphe differium, Hemiphilus, Frysi-erythusum, a roridum, Peronospora manshuriea, Phaeoisariopsis griseola, Phaeosphaeria maydis, Phakopsora gossypii, Phakopsora meibomiae, Phakopsora pachyrhizi, Phoma spp., Phomopsis spp. , Pueeinia triticina, Pyrieularia grisea, Ramularia areola, S. macrospora, Selerotinia sclerotiorum, Septoria glycines, Septoria Iycopersici1.

The formulations of the invention are employed in a single application, sequentially or simultaneously.

For a better understanding of the invention, some examples of formulations used in the present invention are described below, and these examples are intended to exemplify and not to limit the invention. EXAMPLES:

Example 1 - Formulation of the invention (BF488):

(components) _ [concentration (g / 1 OmL)]

Water qsp Silicone emulsion 0.3 Copolymer EO / PO 4 Polyalkylene glycol copolymer with anhydrous polyolefin 1.5 Benzisothiazoline 0.25 Clay smectite 0.35 (components) [concentration (q / 1 OOmDl Monoethylene glycol 7 Gum xanthan 0, 25 Carbendazim 20 Tebuconazole 10 Azoxystrobim 3 Example 2 - Formulation of the invention (RF488) · (components) [concentration (q / 100mül) Water qsp Silicone emulsion 0.3 Copolymer EO / PO 3 Lignosulfonate 2 Isotridecyl alcohol with 12 mo of ethylene oxide 1 Benzisothiazoline 0.25 Clay smectite 0.35 Propylene glycol 5 Xanthan gum 0.25 Carbendazim 20 Tebuconazole 10 Azoxystrobim 3 Example 3 - Formulation of the invention (BF48R): (components) [concentration (q / 100mL) l Water qsp Silicone emulsion 0, 3 Triestyrylphenol phosphate with 16 mo of neutralized ethylene oxide 3 Triestyrylphenol phosphate with 16 mo of ethylene oxide in acid form 2 Isotridecyl alcohol with 12 mo of ethylene oxide 1 (components) No (g / 100mL) 1

Benzisothiazoline 0.25 Clay smectite 0.35 Monoethylene glycol 5 Gum xanthan 0.25 Carbendazim 20 Tebuconazole 10 Azoxystrobim 3 Example 4 - Formulation of the invention CBF488V (components) [Concentration (q / 1 OOmDl Water qsp Silicone emulsion 0.3 Triestyrylphenol phosphate with 16 grams of neutralized ethylene oxide 4.5 Nonylphenol with 10 grams of ethylene oxide 1.7 Benzisothiazoline 0.25 Bentonite clay 0.35 Monoethylene glycol 8.8 Xanthan gum 0.25 Carbendazim 20 Tebuconazole 10 Azoxystrobim 3 Example 5 invention (BF489V (components) [concentration (q / 1 OOmL)] Water qsp Silicone emulsion 0.3 Triestyrylphenol phosphate with 16 mo of neutralized ethylene oxide 4 Isotridecyl Alcohol with 12 mo of ethylene oxide 1.5 Benzisothiazoline 0.25 (components) _ [concentration (g / 1 OmL)!

Bentonite Clay 0.35 Monoethylene Glycol 5 Xanthan Gum 0.25 Carbendazim 20 Flutriafol 6.25 Azoxystrobim 3 Example 6 - Formulation of the Invention IBF489V (Components (concentration (q / 100mL)) Water qsp Silicone Emulsion 0.3 Block Copolymer ethylene oxide and propene oxide 4 Acrylic copolymer 1.5 Benzisothiazoline 0.25 Bentonite clay 0.35 Monoethylene glycol 5 Gum xanthan 0.25 Carbendazim 20 Flutriafol 6.25 Azoxystrobim 3 Example 7 - Formulation of the invention (Components) [ concentration (q / 100mL) 1 Water qsp Silicone emulsion 0.3 Ethene oxide and propene oxide block copolymer 4 Methyl methacrylate copolymer 1.5 Benzisothiazoline 0.25 Bentonite clay 0.35 Monoethylene glycol 5 (components) [concentration (g / 100mL) 1

Xanthan Gum 0.25 Carbendazim 20 Flutriafol 6.25 Azoxystrobim 3 Example 8 - Formulation of the invention (BF489V (components) [concentration (q / 100mL)] Water qsp Silicone emulsion 0.3 Triestyrylphenol phosphate with 16 mo of neutralized ethylene oxide 4.5 Non-ethoxylated phenol with 10 mo of ethylene oxide 1.5 Benzisothiazoline 0.25 Bentonite clay 0.35 Monoethylene glycol 9 Xanthan gum 0.25 Carbendazim 20 Flutriafol 6.25 Azoxystrobim 3

Example 9 - Formulation of the invention (BF465):

(components) _ [concentration (g / 1 OmL)]

Water qsp Polymethylsiloxane 0.2 Triestyrylphenol phosphate with 16 mo of neutralized ethylene oxide 3.0 Ethoxylated isotridecyl alcohol with 6 mo of ethylene oxide 1.0 Benzisothiazoline 0.25 Monoethylene glycol 5.0 Gum xanthan 0.35 (components) _ [ concentration (g / 100mL) 1

Carbendazim 20 Flutriafol 6.25 Krezoxim Methyl 12.5 Example 10 - Formulation of the invention (BF465): (components) [concentration (q / 1 OOmDl Water qsp Polymethylsiloxane 0.2 Ethylene oxide and propene oxide block copolymer 3.0 Acrylic Copolymer 1.0 Benzisothiazoline 0.25 Monoethylene Glycol 5.0 Xanthan Gum 0.35 Carbendazim 20 Flutriafol 6.25 Krezoxim Methyl 12.5 Example 11 - Formulation of the invention CBF465.1: (components) concentration (g / 1 OOmL)] Water qsp Polydimethylsiloxane 0.2 Ethylene oxide and propene oxide block copolymer 3.0 Methyl methacrylate copolymer 1.0 Benzisothiazoline 0.25 Monoethylene glycol 5.0 Gum xanthan 0.35 Carbendazim 20 Flutriafol 6.25 Krezoxim Methyl 12.5

Example 12 - Formulation of the invention (BF452): (components) [Concentration (w / 1 OOmIJl Water qsp Polymethylsiloxane 0.2 Ethene oxide and propene oxide block copolymer 3.0 Methyl Methacrylate copolymer 1.0 Benzisothiazoline 0.25 Monoethylene Glycol 5.0 Xanthan Gum 0.35 Carbendazim 20 Tebuconazole 10 Krezoxim Methyl 12.5

Example 13 - Formulation of the invention (BF452):

(components) _ [concentration (g / 1 OmL)!

Water qsp Polymethylsiloxane 0.2 Triestyrylphenol phosphate with 16 mo of neutralized ethylene oxide 3.0 Ethoxylated isotridecyl alcohol with 6 mo of ethylene oxide 1.0 Benzisothiazoline 0.25 Monoethylene glycol 9.0 Gum xanthan 0.35 Carbendazim 20 Tebuconazole 10 Krez Methyl 12.5

Example 14 - Formulation of the invention (BF452):

(components) _ [concentration (g / 1 OmL)]

Water qsp Silicone Emulsion 0.5 (components) _ [concentration (g / 1 OOmL)]

Phosphate triestyrylphenol with 54 mo of neutralized ethylene oxide 3.0 Ethoxylated Lauric Alcohol with 7 mo of ethylene oxide 1.0 Benzisothiazoline 0.25 Monoethylene glycol 7.0 Xanthan gum 0.30 Carbendazim 20 Tebuconazole 10 Krezoxim Methyl 12.5

Example 15 - Use of the formulations of the invention in cultures of

soy, coffee, rice and cotton, for the control and / or treatment of diseases / pests:

The development of new fungicides and the use of synergistic action between these molecules is an important component in the sustainability of production systems. Although Asian rust has received prominence in recent harvests, other diseases should be considered in the management of soybean crop. Although fungicides used to control rust have a broad control spectrum for most leaf spots, they have low efficiency on target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) and, due to efficient rust control, These diseases are becoming more important in recent harvests. The use of benzimidazole fungicides, such as carbendazin or methyl thiophanate, effectively complements the control of these diseases.

It was observed that the specific synergistic association of three fungicides is an important part in the sustainability of production systems, both in maintaining the efficacy of the crop disease complex and in the management of microorganism resistance. This management of pathogen selection is achieved by the use of three molecules with different modes of action: benzimidazole - acting on the integrity of tubulin; triazole - sterol synthesis inhibitors, ergosterol is the major lipid component of the fungal plasma membrane, having an action in inhibiting ergosterol formation; and strobirulin - inhibit mitochondrial respiration by blocking electron transfer in the cytochrome BC1 site Qo1 complex interferes with ATP formation.

Isolated use of the fungicides tebuconazole, flutriafol, azoxistrobin, kresoxim-methyl and carbendazin is not effective in controlling rust (Phakopsora pachyrhizi Sydow) in soybean (see Tables 1 and 2 below). combination of tebuconazole with carbendazin or flutriafol with carbendazin, which does not provide adequate control in conditions of high disease severity.

The applied fungicides alone were not effective in controlling soybean rust, as can be observed by the high area below the disease progress curve for azoxystrobin even at 60g / ha ai or kresoxim-methyl at 125g / ha of ia (see Table 1 below), carbendazim even at the dose of 250g / ha of ia and tebuconazole at the dose of 100g / ha of ai The poor control efficacy was reflected in lower crop yield. The combination tebuconazole + carbendazim (100 + 200g / ha i.a., respectively) was not effective in controlling soybean rust. The combination of azoxystrobin with the combination of tebuconazole +

Bendazim (BF 488) provided control efficiency gain and residual control effect even under high pressure conditions of Asian rust. This greater residual effect is observed by maintaining low area values below the soybean rust progress curve, which reflected in the best crop yields (see Table 1 below). The addition of azoxystrobin from 6g / ha ai in the tebuconazole + carbendazim mixture (100 + 200 g / ha, respectively) has already reflected in the rust efficiency gain, providing similar control to the azoxistrobin + cyproconazole efficacy standard (60 + 24 g / ha, respectively), this product being marketed by Syngenta as PrioriXtra®. Addition of azoxystrobin at doses of 18, 24 and 30 g / ha of i.a together with tebuconazole + carbenda-10

15

zim was effective in achieving higher than industry standard yields containing 60 g / ha azoxystrobin.

The combination of kresoxim-methyl and the mixture of tebuconazole + carbendazim (BF 452) provided control efficiency gain and residual control effect even under conditions of high Asian rust pressure. This greater residual effect is observed by maintaining low area values below the soybean rust progress curve, which reflected in the best crop yields (see Table 1 below). The addition of kresoxim-methyl from 75 g / ha ai in the tebuconazole + carbendazim mixture (100 + 200 g / ha, respectively) has already resulted in rust efficiency gain, providing similar control to the azoxistrobin efficacy standard. + cyproconazole (60 +24 g / ha, respectively), this product being marketed by Syngenta as PrioriXtra®.

Table 1 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow control. Rondonópolis - MT.

Treatment Common fears

Rate AUPDC * Productivity

g / ha a.i. LowerCanopy UpperCanopy kg / ha

1 azoxystrobrin + cyproconazole

2 kresoxym

3 azoxystrobrin

4 tebuconazole

carbendazin

6 tebuconazole + kresoxym + carbendazin

7 tebuconazole + kresoxym + carbendazin

8 tebuconazole + kresoxym + carbendazin

9 tebuconazole + kresoxym + carbendazin

tebuconazole + kresoxym + carbendazin

11 tebuconazole + carbendazin

12 tebuconazole + kresoxym + carbendazin

13 tebuconazole + kresoxym + carbendazin

14 tebuconazole + kresoxym + carbendazin

tebuconazole + azoxystrobin + carbendazin

16 tebuconazole + azoxystrobin + carbendazin

17 tebuconazole + azoxystrobin + carbendazin

18 tebuconazole + azoxystrobin + carbendazin

19 tebuconazole + azoxystrobin + carbendazin

tebuconazole + carbendazin

21 tebuconazole + azoxystrobin + carbendazin

22 tebuconazole + azoxystrobin + carbendazin

84 (60 + 24) 125 60 100 250

100 + 125+ 250> 100 +100 + 250 100 + 75 + 250 100 + 50 + 250 100 + 25 + 250 I 100 + 250 [100 + 75 + 200 100 + 50 + 200 100 + 25 + 200 100 + 30 +250 100 + 24 + 250 100 +18 + 250 100 +12 + 250 100 + 6 + 250 100 + 200 100 + 18 + 200 100 + 12 + 200

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* area under the curve of disease progress The applied fungicides alone were not effective in controlling soybean rust, as can be seen from the high area below the disease progress curve for azoxystrobin, even at 60 g / ha ai, or kresoxim-methyl at a dose of 125 g / ha ai (see Table 2 below), carbendazim, even at a dose of 250 g / ha ai, and flutriafol at a dose of 62.5 g / ha ai. low control effectiveness was reflected in lower crop productivity. The combination flutriafol + carbendazim (62.5 + 200 g / ha i.a., respectively) was not effective in controlling soybean rust.

The combination of azoxystrobin with the flutriafol + carbendazim mixture (BF 489) provided control efficacy gain and residual control effect even under high pressure conditions of Asian rust. This greater residual effect is observed by maintaining low area values below the soybean rust progress curve, which reflected in the best crop yields (see Table 2 below). The addition of azoxistrobin from 6g / ha ai to the flutriafol + carbendazim mixture (62.5 + 200 g / ha, respectively) has already reflected in the rust efficiency gain, providing similar control to the azoxistrobin + efficacy standard. cyproconazole (60 + 24 g / ha, respectively), this product being marketed by Syngenta as PrioriXtra®. Addition of azoxystrobin at the doses of 18, 24 and 30 g / ha of i.a. together with flutriafol + carbendazim were effective in achieving higher than standard market yields containing 60 g / ha of azoxystrobin.

Table 2 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow control. Rondonópolis - MT. Treatment Cornnon Ffame

1 azoxystrobrin + cyproconazole

2 kresoxym

3 azoxystrobrin

4 float was

carbendazin

6 flutria was + kresoxym + carbendazin

7 flutria was + kresoxym + carbendazin

8 flutria was + kresoxym + carbendazin

9 flutria was + kresoxym + carbendazin

flutria was + kresoxym + carbendazin

11 flutria was + carbendazin

12 flutria was + kresoxym + carbendazin

13 flutria was + kresoxym + carbendazin

14 flutria was + kresoxym + carbendazin

flutria was + azoxystrobin + carbendazin

16 flutria was + azoxystrobin + carbendazin

17 flutria was + azoxystrobin + carbendazin

18 flutria was + azoxystrobin + carbendazin

Flutria was + azoxystrobin + carbendazin

flutria was + carbendazin

Flutria was + azoxystrobin + carbendazin

Flutria was + azoxystrobin + carbendazin

23 flutria was + azoxystrobin + carbendazin

Rate AUPDC *

g / ha a.i. Lower Canopy Upper Canopy

Productivity kg / ha

84 (60 + 24) 125 60 62.5 250

62.5 +125 + 250 62.5 +100 + 250 62.5 + 75 + 250 62.5 + 50 + 250 62.5 + 25 + 250

62.5 + 250 62.5 + 75 + 200 62.5 + 50 + 200 62.5 + 25 + 200 62.5 + 30 + 250 62.5 + 24 + 250 62.5 + 18 + 250 62.5 +12 + 250 62.5 + 6 + 250

62.5 + 200 62.5 +18 + 200 62.5 + 12 + 200 62.5 + 6 + 200

24 Check

Tukey1S HSD (P = .05) CV

16.544 0.61

9.706 0.6

290.574 5.15

The association of kresoxim-methyl with the mixture of flutriafoi + carbendazim (BF 465) provided control efficiency gain and residual control effect even under high pressure conditions of Asian rust. This greater residual effect is observed by maintaining low area values below the soybean rust progress curve, which reflected in better crop yields (see Table 2 above). The addition of kresoxim-methyl from 75 g / ha ai to the flutriafoi + carbendazim mixture (62.5 + 200 g / ha, respectively) has already reflected in the rust efficiency gain, providing similar control to the azoxystrobin efficacy standard. + cyproconazole (60 + 24 g / ha, respectively), this product being marketed by Syngenta as PrioriXtra®.

A study was performed to study the effect of interaction using the multiplicative distribution model proposed by Colby (1967), using Equation 1 below to calculate the expected response:

Equation 1: E = 100 - [(((100 - x) * (100 - y)) / 100] where E is the expected reduction in disease development (such as the reduction in the expected control percentage or the reduction in the area below the disease progress curve), and χ and y represent the reduction in disease development obtained by treatment with the χ and y fungicide, respectively. Possible interaction responses consist of:

a) antagonism, which is characterized by the joint action of two fungicides presenting response of a test organism in their combination,

lower than the expected response, obtained by appropriate reference models, as proposed by Colby (1967);

b) synergism, which is the cooperative action of the two fungicides presenting response in the test organism, in the joint application, superior to

expected from appropriate reference models; and

c) additive, which occurs when two fungicides react with a response in the test organism, when applied together, equal to the expected response obtained by appropriate reference models.

To use the model proposed by Colby, the association tebuconazole + carbendazim (100 + 200, respectively) was set for comparative test with azoxystrobin or kresoxim-methyl and interaction type response (see Table 3 below).

The combination tebuconazole + carbendazim + azoxystrobin (100 + 200 + 30 g / ha i.a., respectively) provided a synergistic effect on their interaction in soybean rust control, as can be seen in Table 3 below. This synergistic interaction was effective in controlling the disease, both in the leaves of the soybean plant and in the leaves of the pointer. The synergism in the control of the disease in the leaves of the plant's leaves reflects high control efficiency, since the applied products reach the leaves of this position of the plant in a smaller quantity and the disease has a better condition for its development.

The combination tebuconazole + carbendazim + kresoxim-methyl (100 + 200 + 125 g / ha i.a., respectively) provided a synergistic effect on their interaction in soybean rust control, as can be seen in Table 3 below. This synergistic interaction was effective in controlling the disease, both in the leaves of the soybean plant and in the leaves of the pointer. The synergism in the control of the disease in the leaves of the plant's leaves reflects high control efficiency, since the applied products reach the leaves of this position of the plant in a smaller quantity and the disease has a better condition for its development.

Table 3 - Phakopsora pachyrhizi control efficacy obtained by the area under the disease progress curve (AUDPC) in the lower canopy and upper canopy as a function of fungicide interactions and the expected control (Colby). Rondonópolis - MT._

Treatment Rate Control (%)

Roof

Common name i.a./ha V,. Topcoverings

lower r

1 Kresoxim-methyl 125 13 34 2 Azoxystrobin 30 12 57 3 Tebuconazole 100 32 71 Tebuconazole + Carbendazim + Kresoxim 100 + 200 + 125 74 (64.3) * + 94 (90.8) + _ Tebuconazole + Carbenda- 5 zim + Azoxystrobin 100 + 200 + 30 77 (63.9) + 98 (94.0) + „Tebuconazole + Carbenda- 6 zim 100 + 200 59 86 7 Control 0 0 LSD (P = .05) 1.41, 4 CV 2.38 1.48

* values in parentheses are the expected values calculated by Colby's equation (1967). Negative sign after expected value indicates effect

antagonistic in interaction, positive sign means synergistic effect, and no signal indicates additive interaction.

For the study of the interaction effect for the combination of flutriafol + carbendazin + azoxystrobin and flutriafol + carbendazin + kresoximethyl, the model proposed by Colby was used. To this end, the association flutriafol + carbendazim (62.5 + 200, respectively) was set for comparative test with azoxystrobin or kresoxim-methyl and response type interaction (see Table 4 below).

The association of flutriafol + carbendazim + azoxystrobin (62.5 + 200 + 30 g / ha i.a., respectively) provided a synergistic effect on their interaction in soybean rust control, as can be seen in Table 4 below. This synergistic interaction was effective in controlling the disease, both in the leaves of the soybean plant and in the leaves of the pointer. The synergism in the control of the disease in the leaves of the plant's leaves reflects high control efficacy, since the applied products reach the leaves of this position of the plant in a smaller quantity and the disease has a better condition for its development.

The combination of flutriafol + carbendazim + kresoxim-methyl (62.5 + 200 + 125 g / ha i.a., respectively) provided a synergistic effect on their interaction in soybean rust control, as can be seen in Table 4 below. This synergistic interaction was effective in controlling the disease, both in the leaves of the soybean plant and in the leaves of the pointer. The synergism in the control of the disease in the leaves of the plant's leaves reflects high control efficiency, since the applied products reach the leaves of this position of the plant in a smaller quantity and the disease has a better condition for its development.

Table 4 - Phakopsora pachyrhizi control effectiveness obtained

by the area below the disease progress curve (AUDPC) in the lower canopy and upper canopy as a function of fungicide interactions and the expected control (Colby). Rondonópolis - MT. _

Treatment Rate Control (%) Common name g ia / ha Lower coverage Upper or upper coverage 1 Kresoxim-methyl 125 18,0 15,5 2 Azoxystrobin 30 19,8 20,0 3 Flutriafol 62,5 24,0 26,5 4 Flutriafol + Carbendazim + Kresoxim 62.5 + 200 + 125 50.9 (45.1) * + 72.7 (65.4) + Flutriafol + Carbendazim + Azoxystrobin 62.5 + 200 + 30 58.8 (46.3 ) + 84.2 (67.3) + 6 Flutriafol + Carbendazim 62.5 + 200 33.0 59.1 7 Control 0.0 0.0 LSD (P = .05) 0.85 0.83 CV 1, 97 1.41

* values in parentheses are the expected values calculated by Colby's equation (1967). Negative sign after expected value indicates antagonistic effect on interaction, positive sign means synergistic effect, and absence of signal indicates additive interaction. 10

15

20

The combination of azoxystrobin with tebuconazole with carbendazim or the combination of kresoxim-methyl with tebuconazole with carbendazim provides high rust control efficacy (see Table 1 above) and this interaction has synergistic effect (see Table 3 above). . In addition to these benefits, the combination of the three fungicides tebuconazole, carbendazin and azoxystrobin or tebuconazole, carbendazin and kresoxim-methyl provides greater protection against fungicide-resistant fungi and improved control spectrum. . The increase in the control spectrum can be seen in Table 5 below by the high efficacy of target spot (Corynespora cassiicola) and anthracnesis (Colletotrichum truncatum) control.

Table 5 - Mean results of target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) severity at 14 days after the third application due to fungicide interactions. Rondonópolis - MT._

Treatment Common fears

Rate g / ha to C.L

Severily (%) cassiicola C. truncatum

1 azoxystrobrin + cyproconazole

2 kresoxym

3 azoxystrobrin

4 Tebuconazole

Flutriafol

6 tebuconazole + kresoxym + carbendazin

7 flutriafol + kresoxym + carbendazin

8 tebuconazole + kresoxym + carbendazin

9 fiutriafol + kresoxym + carbendazin

tebuconazole + azoxystrobin + carbendazin

11 fiutriafol + azoxystrobin + carbendazin

12 tebuconazole + azoxystrobin + carbendazin

84 (60 + 24) 125 60 100 62.5

100 + 125 + 200 'ψ 62.5 + 125 + 200 100 +25 +200 62.5 + 25 + 200 100 + 30 + 200 62.5 +30 +200 100 + 6 + 200

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14 Check ■ y- H S £ £ 6Q βla Tukey1S HSD (P = .05) 4,459 4,617 HP 13.58 15.35

* target spot (Corynespora cassiicola); ** anthracnose (Colletotrichum truncatum)

The association of azoxystrobin with fiutriafol with carbendazim or the association of kresoxim-methyl with fiutriafol with carbendazim provides high rust control efficacy (see Table 2 above) and this interaction has a synergistic effect (see Table 4 above). In addition to these benefits, the combination of the three fiutriafol, carbendazin and azoxystrobin or flutriafol, carbendazin and kresoxim-methyl fungicides provides greater protection against the emergence of fungicide-resistant fungi and improves the control spectrum. The increase in the control spectrum can be seen in Table 5 above by the high efficacy of target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) control.

The soybean target spot is caused by the fungus Corynespora cassicola (Berk. & M.A. Curtis) C.T. Wei. This pathogen was first identified in the US in 1945 as Helminthosporium vignae. In Brazil, the first records were in Mato Grosso in 1974 and Paraná in 1976 (Almeida et al., 1976). Severe but sporadic outbreaks have been observed in the colder regions of the south and in the upper regions of the Cerrados (Tecnologia ..., 2008). It has taken on great expression in recent harvests, especially in Tocantins and Mato Grosso, where it occurs most severely, however, it occurs in soybean areas throughout the cerrado.

The fungus is found in practically all soybean growing regions of Brazil, believed to be native and to infect a large number of plant species. It can survive in crop residues and infected seeds, which is a form of spread. High relative humidity conditions and mild temperatures are favorable for leaf infection. The most common symptoms are leaf spots with a yellowish halo and dark punctuation in the center that cause severe defoliation. Stains also occur on the stem and pod. The fungus can also infect roots, causing root rot and intense sporulation (Henning et al., 2005). Another disease that is becoming increasingly important in the

soybean is anthracnose, which is caused by the fungus Colletotrichum dematium (Pers. Ex Fr.) Grove var. truncata (Schw) Arx (sin. C. truncatum (Schw.) Andrus & Moore. Anthracnose is one of the main diseases in the crop in all stages of development from the seedling stage to the early stage of pod formation. In rainy years, it can cause total production loss, with a high reduction in the number of pods (Yorinori, 1997) .The highest intensities of anthracnose occurrence in the Cerrado have been attributed to high rainfall. The use of infected seeds also contributes to the higher occurrence of the disease, and a considerable increase in the occurrence of Coltotrichum truncatum has been observed in soybean seeds evaluated by the blotter test. who suffered a delayed harvest due to rainfall, presented infection rates higher than 50% (Almeida et al., 1997).

The occurrence of soybean rust from the 2001/2002 harvest led producers and technicians to divert their attention from other important diseases, such as anthracnose and target spot. Several strategies are recommended for disease control, such as the use of resistant cultivars, seed treatment, crop rotation / succession with maize and grass species and fungicide sprays (Almeida et al. 1997; Henning et al., 2005; Yorinori et al., 2010). However, fungicides commonly used in soybean rust management have less effectiveness in controlling these diseases.

Isolated use of the fungicides tebuconazole, flutriafol, azoxistrobin and kresoxim-methyl is not effective in controlling target spot (C. cassiola) and anthracnose (C. truncatum) in soybean (see Table 5 above) as well. such as the use of standard fungicide for soybean rust control, the association of azoxystrobin with cyproconazole (PrioriXtra®), which does not provide adequate control in conditions of high disease severity.

The applied fungicides alone were not effective in the control of soybean target spot and anthracnose, as can be observed by the high disease severity for azoxystrobin even at the dose of 60 g / ha of ia or kresoxim-methyl at the dose of 125 g / ha of ia (see Table 5 above).

The combination of azoxystrobin with the tebuconazole + carbendazim mixture provided control efficacy gain and residual control effect even under conditions of high target spot pressure and anthracnose. This greater control effect is observed by the lower severity of disease (see Table 5 above). The addition of azoxystrobin from 6 g / ha ai in the mixture of tebuconazole + carbendazim (100 + 200 g / ha, respectively) has already reflected in the efficacy of target spot and anthracnose, providing controls superior to the efficacy standard. azoxystrobin + cyproconazole (60 + 24 g / ha, respectively) (PrioriXtra®).

The combination of kresoxim-methyl with the mixture of tebuconazole + carbendazim provided control efficiency gain and residual control effect, even under conditions of high target spot pressure and anthracnose. This greater residual effect is observed by maintaining low severity values (see Table 5 above). The addition of kresoxim-methyl from 25 g / ha ai in the tebuconazole + carbendazim mixture (100 + 200 g / ha, respectively) has already reflected in the efficacy of target spot and anthracnose, providing superior control over azoxystrobin + cyproconazole. (60 + 24 g / ha, respectively) (PrioriXtra®). The combination of azoxystrobin with the flutriafol + carbenda-

zim provided gain in control efficacy and residual control effect even under conditions of high target spot pressure and anthracnose. This greater control effect is observed by the lower severity of disease (see Table 5 above). The addition of azoxystrobin from 6 g / ha ai in the mixture of flutriafol + carbendazim (62.5 + 200 g / ha, respectively) has already reflected in the target spot and anthracnose efficacy gain, providing superior control to the efficacy standard. azoxystrobin + cyproconazole (60 + 24 g / ha, respectively) (PrioriXtra®).

The association of kresoxim-methyl with the flutriafol + carbendazim mixture provided control efficiency gain and residual control effect, even under conditions of high target spot pressure and anthracnose. This greater residual effect is observed by maintaining low severity values (see Table 5 above). The addition of kresoxim-methyl from 25 g / ha ai to the flutriafol + carbendazim mixture (62.5 + 200 g / ha, respectively) has already reflected in the efficacy of target spot and anthracnose, providing superior control over azoxystrobin. + cyproconazole (60 + 24 g / ha, respectively) (PrioriXtra®). In intensive production systems there is greater disease pressure as well as the emergence of new diseases that were not important, leading to production losses. In recent years this fact has been observed in soybean culture mainly with the target spot and anthracnose diseases. Tebuconazole alone is not effective in controlling these diseases even at twice the commercial dose, similarly to azoxystrobin in soybean. The combination of azoxystrobin with the mixture of tebuconazole and carbendazim provides adequate levels of control of both target and anthracnose.

The combination of azoxystrobin with tebuconazole with carbendazin

provides high rust control effectiveness (see Table 6 below). Addition of azoxystrobin provides synergistic effect on Asian rust control. The combination of the three fungicides tebuconazole, carbendazim and azoxystrobin provides target spot control efficacy (Corynespora

cassiicola) and anthracnose (Colletotrichum truncatum) (see Table 5 above), besides containing fungicides with three modes of action - carbendazin (benzimidazole) - acting on the integrity of tubulin; tebuconazole (triazole) - sterol synthesis inhibitors; azoxystrobin (strobirulin) - inhibits mitochondrial respiration.

Table 6 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow control. Rondonópolis - MT._

Treatment Common Name

Rate AUPDC * Productivity

g / ha a.i. Lower Canopy Upper Canopy kg / ha

1 azoxystrobrin + cyproconazole

2 kresoxym

3 azoxystrobrin

4 Tebuconazole

carbendazin

6 tebuconazole + kresoxym + carbendazin

7 tebuconazole + kresoxym + carbendazin

8 tebuconazole + kresoxym + carbendazin

9 tebuconazole + kresoxym + carbendazin

tebuconazole + kresoxym + carbendazin

11 tebuconazole + azoxystrobin + carbendazin

12 tebuconazole + azoxystrobin + carbendazin

13 tebuconazole + azoxystrobin + carbendazin

14 tebuconazole + azoxystrobin + carbendazin

tebuconazole + azoxystrobin + carbendazin

16 tebuconazole + carbendazin

84 (60 + 24) 125 60 100

250 __

100 +125 + 200 [v / t, 391.4j and 100 +100 + 200 414.9 and 100 + 75 + 200 100 + 50 + 200 100 + 25 + 200 100 + 30 + 200 100 + 24 + 200 100 + 18 + 200 100 + 12 + 200 100 + 6 + 200 100 + 200

56® ghi 074ji1 'c

etlgiíd

227.5 and

IfSf b 57 * 5; ghi tílileSshi

81.8 fg 107.4 f 195.7 and

2889.8 bc

2806.3 cd

2865.4 bc 2757.3 cde 2719.6 cde

La

1 * 9

; 406.6 and t645.2 c

Rf (6 * 3; hi

** wyra, ^ 39.3 hi

51.8 ghi 66; í | gh '220.3 and

17 Check

2881.9 bc 2820.5 and 2608.8 of

Tukey1S HSD (P = .05) CV

58.406 3.48

37.538 4.19

205.97 3.18 * area under the curve of disease progress The association of kresoxim-methyl with tebuconazole with carbendazin provides high rust control efficacy (see Table 6 above). The addition of kresoxim-methyl provides synergistic effect on Asian rust control. The combination of the three fungicides tebuconazole, carbendazim and kresoxim-methyl provides effective control of target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) (see Table 5 above), and contains fungicides with three modes of action - carcid. - bendazin (benzimidazole) - acting on integrity of tubulin; tebuconazole (triazole) - sterol synthesis inhibitors; azoxystrobin (strobirulin) - inhibits mitochondrial respiration.

Similarly, the combination of azoxystrobin with flutriafol with carbendazin as well as the association of kresoxim-methyl with flutriafol with carbendazin provides high rust control efficacy (see Table 2 above). The addition of azoxystrobin and kresoxim-methyl to flutriafol with carbendazin provides a synergistic effect on controlling asian rust. The combination of the three flutriafol, carbendazim and azoxystrobin or flutriafol, carbendazim and kresoxim-methyl fungicides provides target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) control efficacy (see Table 5 above) and contains three-mode fungicides. of action - carbendazin (benzimidazole) - acting on integrity of tubulin; flutriafol (triazole) - sterol synthesis inhibitors; azoxystrobin and kresoximethyl (strobirulin) - inhibits mitochondrial respiration.

For a better observation of the innovation brought about in accordance with the present invention, BF 488 (tebuconazole 100 + carbenazin 200 + azoxystrobin 30) is compared with the standard commercial PrioriXtra® (Syngenta brand) market products and Opera® (trademark by Basf) (see Tables 7 and 9 below). BF 488 is effective in controlling soybean rust, exceeding market standards. The BF 488 provided better control of rust on the leaves of the soybean, median third and pointer of the soybean plant, reflected by its high efficacy and better residual control. The better disease control provided by the synergistic effect of the balanced association of BF 488 molecules (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) can be observed in the best yield and greater mass of 1,000 soybeans, even when compared. to PrioriXtra ® (azoxystrobin + cyproconazole, 60 + 24 g / ha, respectively).

Table 7 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi control Sydow, Luis Eduardo Magalhães - BA.

1 UNTR EATED 2 ProduetX 3 ProduetY 4 BF 488-01 100 + 200 + 30 BF 488-01 120 + 240 + 36 6 PRIOR! XTRA 60 + 24 7 OPERATES 66.5 + 25

t 843.4 b

JtTi

92.5- c J 982.3 bc:

HBBiSiBjjaa

503.1 b 791.9 b ϋθΤί bcd 743.6 b

Igigfcd IIIgPjcdi

476.2 bt I 718.6 bc (4S6Fla La Pi3 '| 93; g abj 474.6 bc 741.8 b 133.8 to 3771.3 b

LSD (P = .Q5)

CV

88.3 124.66 5.7 9.73

79.21

9.42

81.59

6.67

12,184

6.22

262,439

4.84

* Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all treatments.

For a better observation of the innovation brought about in accordance with the present invention, BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) is compared with the standard commercial products PrioriXtra® (trademark by Syngenta) and Opera® ( brand marketed by BASF) (see Tables 8 and 9 below). BF 452 is effective in controlling soybean rust, exceeding market standards. The BF 452 provided better rust control in the leaves of the shoal, middle third and pointer of the soybean plant, reflected by its high efficacy and better residual control. The better disease control provided by the synergistic effect of the balanced association of BF 452 molecules (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) can be observed in the best soybean yield, even when compared to PrioriXtra® (azoxistrobin). + cyproconazole, 60 + 24 g / ha, respectively).

For a better observation of the innovation brought about in accordance with the present invention, BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) is compared with the market standard Prio-10 commercial products.

15

riXtra® (trademark from Syngenta) and Opera® (trademark from Basf) (see Tables 8 and 9 below). BF 465 is effective in controlling soybean rust and is similar to industry standards. The BF 465 provided excellent rust control in the leaves of the shoal, middle third and pointer of the soybean plant, reflected by its high efficacy and better residual control. The better disease control provided by the synergistic effect of the balanced association of BF 465 molecules (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) can be observed in better soybean yield, even when compared to PrioriXtra® (azo - xistrobin + cyproconazole, 60 + 24 g / ha La., respectively).

Table 8 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow control, Rio Verde - GO.

* Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all treatments.

Table 9 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in the control

and Phakopsora pachyrhizi Sydow, Santa Helena de Goiás - GO

1370,> 9'f

mt-t vi— 1414.9 and

HSBStfcSW-

12021.3 'b

1922.8 ab 1992.1 ab

# 2176, 9lab 12.54 * Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all treatments.

For a better understanding of the innovation brought about in accordance with the present invention, BF 489 (flutriafol 62.5 + carbendazim 200 + azoxystrobin 30) is compared with the standard commercial products PrioriXtra® (Syngenta brand) and Opera ® (trade mark by Basf) (see Table 9 above). BF 489 is effective in controlling soybean rust, even at high disease pressure, and is superior to industry standards. BF 489 provided better control of rust on the leaves of the shoal, middle third and pointer of the soybean plant, reflected by the high efficacy and better control residual. The better disease control provided by the synergistic effect of the balanced association of BF 489 molecules (flutriafol 62.5 + carbendazim 200 + azoxystrobin 30) can be observed in the better soybean yield, even when compared to Prirrixtra®. (azoxystrobin + cyproconazole, 60 + 24 g / ha, respectively) and Opera®.

The synergistic association of azoxystrobin with tebuconazole with carbendazim provides high rust control efficacy (Tables 1, 6, 7, 9, 10, 12 and 13) and this interaction has synergistic effect (Table 3). In addition to these benefits, the combination of the three tebuconazole, carbendazin and azoxystrobin fungicides provides greater safety in protecting against the emergence of fungicide-resistant fungi and enhances the control spectrum.

The synergistic association of azoxystrobin with flutriafol with carbendazim provides high rust control efficacy (Tables 2, 9, 10, 12 and 13) and this interaction has a synergistic effect (Table 4). In addition to these benefits, the combination of the three flutriafol, carbendazin and azoxistrobin fungicides provides greater safety in protecting against the emergence of fungicide-resistant fungi and enhances the control spectrum.

The synergistic association of kresoxim-methyl with tebuconazole with carbendazim provides high rust control efficacy (Tables 1, 6, 8, 9, 11 and 12) and this interaction has synergistic effect (Table 3). In addition to these benefits, the combination of the three fungicides tebuconazole, carbendazin and kresoxim-methyl provides greater safety in protecting against the emergence of fungicide-resistant fungi and improves the control spectrum.

The synergistic association of kresoxim-methyl with flutriafol with

Bendazim provides high rust control efficacy (Tables 2, 8, 9, 11 and 12) and this interaction has synergistic effect (Table 4). In addition to these benefits, the combination of the three flutriafol, carbendazin and kresoxim-methyl fungicides provides greater safety in protecting against the emergence of fungicide-resistant fungi and enhances the control spectrum.

This synergistic association of three molecules with a balanced relationship provides high efficacy in controlling important and difficult-to-manage diseases in soybean such as target spot (Corynespora cassiicola) and anthracnose (Colletotrichum truncatum) (Table 5 above). In addition to providing high control efficacy of septoriosis (Septoria glycines) (Table 14 below), cercosporiosis (Cercospora kikuchii) (Table 15 below) and white mold (Sclerotinia sclerotiorum) (Table 16 below) on soybean crop.

This synergistic interaction provides increased spectrum of disease control in crops with the application of BF 488 (tebuconazole + carbendazim + azoxystrobin), BF 489 (flutriafol + carbendazim + azoxystrobin), BF 452 (tebuconazole + carbendazim + kresoxim) products. methyl) and BF 465 (flutriafol + carbendazim + kresoxim-methyl), with no need for product mixing in the spray tank as well as immediate re-entry into the area with another product. This is due to the synergistic association of three modes of action of fungicides, triazoles - which have high efficacy in controlling Ascomycides and Basidiomycetes and certain activity in Deuteromycetes, benzimidazoles - which have high efficacy in controlling Deuteromycetes and certain activity in the control of Ascomycetes and Basidiomycetes, and strobirulins - which have high efficacy in Deuteromycetes and certain activity in Oomycetes and Basidiomycetes and little activity in Ascomycetes. Thus, the synergistic interactions BF 488 (tebuconazole + carbendazim + azoxystrobin), BF 489 (flutriafol + carbendazim + azoxystrobin), BF 452 (tebuconazole + carbendazim + kresoxim-methyl) and BF 465 (flutriafol + carbendazim + kresox -methyl) provide high efficacy of disease complex control in maize crop, such as: Helmintosporiosis (Exerohilum turcicum) (Table 22 below); leaf spot or phosphorus (Phaeosphaeria maydis) (Table 24 below); diplodia spot (Diplodia macrospora); cercosporiosis (Cercospora zea-maydis) (Table 21 below); Anthracnose (Colletotrichum graminicola) (Tables 17, 19 and 25 below); polishing rust (Puccinia polysora) (Table 20 below); tropic rust (Physopella zeae) (Table 18 below); common rust (Puccinia sorghi) (Tables 17 and 23 below).

The synergistic interactions BF 488 (tebuconazole + carbendazim + azoxystrobin), BF 489 (flutriafol + carbendazim + azoxystrobin), BF 452 (te-buconazole + carbendazim + kresoxim-methyl) and BF 465 (flutriafol + carbendazim + kresoxim-methyl) provide high efficacy of controlling the disease complex in rice crop, such as: blast (Pyricularia grisea) and brown spot (Bipolaris oryzae) (Tables 26, 27 and 28 below).

The synergistic interactions BF 488 (tebuconazole + carbendazim + azoxystrobin), BF 489 (flutriafol + carbendazim + azoxystrobin), BF 452 (te-buconazole + carbendazim + kresoxim-methyl) and BF 465 (flutriafol + carbendazim + kresoxim-methyl) provide high control efficacy of major cotton crop leaf diseases in Brazil, such as: Ramulose (Colletotrichum gossypii var. cephalosporioides) (Tables 32 and 33 below); Ramularia (Ramularia areola Atk.) (Tables 29, 30 and 31 below); Alternaria Spot (Alternaria sp); Mirothecium (Myrothecium roridum); Rust (Phakopsora gossypii) (Puccinia cacabata).

The high efficacy of the synergistic interactions BF 452 (tebuconazole + carbendazim + kresoxim-methyl) and BF 465 (flutriafol + carbendazim + kresoxim-methyl) can also be observed in control of coffee rust (Hemileia vastatrix) (Table 34 below). and ascochyta stain (Table 35 below) provide high efficacy of disease complex control in coffee crop.

The development of new products using the synergistic action between molecules is an important component in the sustainability of production systems. Providing improved control levels of plant diseases, gain of control spectrum, greater difficulty in the emergence of fungicide resistance and greater safety regarding the use of fungicides.

Example 16: Effectiveness of BF 488 and BF 489 in controlling Phakopsora pachyrhizi Sydow in soybean crop, Santa Helena de Goiás / GO, 2009/2010 crop:

The present work was carried out to evaluate the efficacy of the products in the control of Asian soybean rust, the preventive application initiation in R1, the second application being performed 20 days after the first and the third application 15 days after the application. Monday. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v and Fox in which Aureo was added. at a dose of 0.4 L / ha. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) and BF 489 (flutriafol 62.5 + carbendazim 200 + azoxystrobin 30) are effective in controlling soybean rust (Table 10 below), providing excellent residual control that can be observed by the smallest area below the rust progress curve. The efficacy of BF 488 and BF 489 is consistent, reducing disease throughout the soybean plant canopy. The best treatments were the BPC 488, BF 489, Fox and PrioriXtra, which showed better mobility and / or greater residual efficacy of these fungicides. Analyzing the pointer leaf AUDPC, the best treatments were BF 488 and AproachPrima, demonstrating the high activity of these fungicides in disease control. The best yields were observed in treatments with BF 488, BF 489, Opera, AproachPrima and Fox. The applied tebuconazole alone was not effective in controlling soybean rust. Table 10 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi control Sydow, Santa Helena de Goiás-GO.

* Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all

of treatments, except treatment 6, in which 0.5% Nimbus was added, and treatment 11, in which 0.4 Uha of gold was added.

Example 17: Effectiveness of BF 452 and BF 465 in controlling Phakopsora pachyrhizi Sydow in soybean crop. Rio Verde / GO, Crop 2008/2009.

The present work was performed to evaluate the efficacy of the products in the control of Asian soybean rust (Favorita). The beginning of preventive applications in R1, the second application being performed 19 days after the first (in R4) and the third application 15 days after the second (in R5.3). Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling soybean rust (Table 11 below) , providing excellent residual control that can be observed by the smallest area below the rust progress curve. The efficacy of BF 452 and BF 465 are consistent, reducing disease throughout the soybean plant canopy. Analyzing the AUDPC of the leaves of the bass, the best treatments were BF 452, BF 465 and Opera, demonstrating better mobility and / or greater residual efficacy of these fungicides. The best treatments were the BF 452 and BF 465 treatments, showing the high activity of these fungicides in controlling the disease. The best yields were observed in the treatments with BF 452, BF 465 and PrioriXtra. The applied tebuconazole alone was not effective in controlling soybean rust.

Table 11 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in the control

* Assist added 0.5% v / v in all treatments.

Example 18: Effectiveness of BF 452, BF 465, BF 488 and BF 489 in the control of Phakopsora pachyrhizi Sydow in soybean culture, Goiânia / GO. 2009/2010 harvest.

The present work was performed to evaluate the efficacy of the products in the control of Asian soybean rust (Valiosa). The start of preventive applications on R1, the second application being performed 19 days after the first (in R5.1) and the third application 15 days after the second (in R5.5). Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments, where 0.5% v / v Nimbus was added. BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125); BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30); product BF 465 (flutriafol 62,5 + carbendazim 200 + kresoxim-methyl 125) and product BF 489 (flutriafol 10

15

20

62.5 + carbendazim 200 + azoxystrobin 30) are effective in controlling soybean rust (Table 12 below), providing excellent residual control, which can be observed by the smaller area below the rust progress curve. The efficacy of BF 452, BF 465, BF 488 and BF 489 are consistent, reducing disease throughout the soybean plant canopy. Analyzing the AUDPC of the leaves of the bass, the best treatments were BF 452, BF 488, BF 489 and PrioriXtra, demonstrating better mobility and / or greater residual efficacy of these fungicides. Analyzing the AUDPC of the tip leaves, the best treatments were BF 452, BF 465, BF 488, BF 489, PrioriXtra and Opera, demonstrating the high activity of these fungicides in disease control. The best yields were observed in the treatments with BF 452, BF 465, BF 488, BF 489, Opera and PrioriXtra. The applied tebuconazole alone was not effective in controlling soybean rust.

Table 12 - Area under the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow, Goiânia - GO._

Productivity?

'Treatment

ν RateghalaLif

AUDPC

LEALÓW LEAtilID 'LEAÜ ^ gpÍATOT kg ago

* Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all treatments.

Example 19: Effectiveness of BF 488 in the control of Phakopsora pachyrhizi Sydow in soybean crop, Goiânia / GO, 2009/2010 crop.

The present work was performed to evaluate the efficacy of the products in the control of Asian soybean rust. The beginning of preventive applications in V9, the second application being performed 20 days after the first and the third application 15 days after the second. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra1 treatments where Nimbus 0.5% v / v was added and Fox in which Aureo was added in the treatment. 0.4 L / ha. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) and BF 489 (flutriafol 62.5 + carbendazim 200 + azoxystrobin 30) are effective in controlling soybean rust (Table 13 below), providing excellent residual control, which can be observed by the smallest area below the rust progress curve. The efficacy of BF 488 and BF 489 is consistent, reducing disease throughout the soybean plant canopy. Analyzing the AUDPC of the leaves of the bass, the best treatments were BF 488, BF 489, Fox and AproachPrima, demonstrating better mobility and / or greater residual efficacy of these fungicides. Analyzing the pointer leaf AUDPC, the best treatments were BF 488, BF 489 and AproachPrima, demonstrating the high activity of these fungicides in disease control. The best yields were observed in treatments with BF 488, BF 489, PrioriXtra, Opera, AproachPrima and Fox. The applied tebuconazole alone was not effective in controlling soybean rust.

Table 13 - Area below the disease progress curve (AUDPC) and productivity as a function of fungicide interactions in Phakopsora pachyrhizi Sydow control, Goiânia-GO _

^ »AU WGdu & eTSpAi- IlProductivitY 3

and LEALOW ISJLEAMILEAU PPfofflRLATQT.

LSD (P = .05) 75.6 70.9 55.5 43.7 | 852533, Oj CV 2.3 3.3 3.8 1.9 | 18.3 | * Addition of Assist (emulsifiable mineral oil) 0.5% v / v in all treatments except treatment 6, where 0.5% Nimbus was added, and treatment 11, where 0 was added, 4 L / ha of Golden.

Example 20: Effectiveness of BF 488 in the control of Septoria glycines soybean crop, Goiânia / GO, 2009/2010 crop.

The present work was performed to evaluate the efficacy of products in the control of soy septoriosis. This was installed in stainless steel soybean TMG 801 to avoid interference of soybean rust occurrence in the evaluation of the experiment. The beginning of the applications was preventive in R1, being the second application 21 days after the first. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where 0.5% v / v Nimbus was added. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) is effective in controlling Septoria glycines (Table 14 below), providing excellent residual control, which can be observed by the smaller area below the progress curve. of the disease. The effectiveness of BF 488 is consistent, reducing disease throughout the soybean plant canopy. The severity of the disease was high as can be seen in the untreated control with 54% severity at 48 days after the second application (DA2A). Analyzing septoriosis severity at 17 DA2A, the best treatments were BF 488, PrioriXtra and PrioriXtra + Portero. However, in assessing severity at 58 DA2A, BF 488 maintains better control. The best control effectiveness and maintenance of residual effect of BF 488 can be observed in the lowest AUPDC values. Table 14 - Area below the disease progress curve (AUDPC) and severity as a function of fungicide interactions in Septoria glycines control, Goiânia-GO._

«ΡΙ & ΜΙΜΉ fHH« H '^ Msej ^ / iic IIISÍDAÍASI glyaries {Sévt Pií37bÀ2ÀWí iK | §t§ ||| ji § »58DA2A1S 1! UNTREATED 16 to 19 to 28 to 54 to 58 to 351 Ba 2; BF 488-01 100 + 200 + 30 5 bcd 9 cd 13 d 17: c 38 bc IpBapf 3; BF 488-01 120 + 240 + 36 5 cd 9 cd 14 cd 14; C 41 bc SffigBjWmqgj 4 | PORTER 400 7 b 9 cd 13 d 24ib 38 bc 6811 of 5! PRIORI XTRA 60 + 24 5 bcd 9 cd 15 bcd 23 b 43 bc 706.3 cc 6! PRIORI XTRA + PORTER 60 + 24 + 400 5 cd 10 C 19 b 26ib 4? b 7971 b LSD (P = .05) 1.9 2.5 3.5 4.9 Oj 79.57 CV 22.34 17.01 15.38 14.57 9.51 7.31 10

15

20

Example 21: Effectiveness of BF 488 in the control of Cercospora kikuchii soybean crop, Goiânia / GO, 2009/2010 crop.

The present study was carried out to evaluate the efficacy of products in soybean cercosporiosis control. It was installed in stainless steel TMG 801 to avoid interference of soybean rust occurrence in the evaluation of the experiment. The beginning of the applications was preventive in R1, being the second application 21 days after the first. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtral treatments where Nimbus 0.5% v / v was added. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) is effective in controlling Cercospora kikuchii (Table 15), providing excellent residual control, which can be observed by the smallest area below the disease progress curve. The effectiveness of BF 488 is consistent, reducing disease throughout the soybean plant canopy. The severity of the disease was high, as can be seen in the untreated control with 29% severity at 48 days after the second application (DA2A). Analyzing the severity of cercosporiosis at 29 DA2A, the best treatments were BF 488, Portero, PrioriXtra and PrioriXtra + Portero. However, in assessing severity at 58 DA2A, BF 488 maintains better control. The best control effectiveness and maintenance of residual effect of BF 488 can be observed in the lowest AUPDC values.

Table 15 - Area below the disease progress curve (AUDPC) and severity as a function of fungicide interactions in Cercospora kikuchii control, Goiânia-GO- _

IRETREATED 3 to 7 to 13 to 21 2: BF 488-01 100 + 200 + 30 0 C 3 and 6 d 6 efj 12 ° C ^^^ Bef 3; BF 488-01 120 + 240 + 36 2 b 4 of 4 and 4 fgj 131 bcálllÉKpft ef 4; PORTER 400 0 C 5 b 8, bc 9 bc 16! b 299 8 bc 5; PRIORIXTRA 60 + 24 0 C 5 b 7 cd 9 cd 13 bcc 269 8 cd 6: PRIORI XTRA + PORTER 60 + 24 + 400 0 C 5 b 9 b 11 b 15! bc '3285 b' LSD (P - .05) 1.1 0.9 1.6 2.4 3.3 43.97 CV 151.05 13.87 16.31 20.03 15.3 11.23 |

25

Example 22: Effectiveness of BF 452 and BF 488 in the control of Sclerotinia sclerotiorum soybean crop, Barreiras / BA, 2009/2010 crop: The trial was performed in a randomized block design with four replications. This was carried out in Barreira-BA1 in the 2009/2010 crop, in the cultivar M-Soy 9144RR. Fungicide application started at 25 days after culture emergence (DAE) 1 with the second application occurring at 35 DAE and the third application 15 days later on R2. Analyzing the incidence of white mold at 95 DAE, the fungicides BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30), BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125), Frowncide and Sumilex were effective in controlling the disease. disease, not differentiating (Table 16 below). However, analyzing soybean yield, it was observed that there was no difference between these fungicides, and they were effective in controlling Sclerotinia sclerotiorum in soybean crop.

Table 16 - Incidence and severity of Sclerotinia sclerotiorum on soybean crop and yield as a function of fungicide interactions, Barreiras-BA.

te £ r'Treatment * 'üarne Ra ^ igjhas | ag | g SJriclçlenÇ W- Sdemtmiasderptiorum everity, 9i Productiv ty 1 UNTREATED 1 a! 3 a ^ ÉÈZtSLL iüü ^ a? BF 452-07 100 + 200 + 125 0 i 2 ah Pfcesfsia 2577.8 to 3 BF 452-07 200 + 400 + 250 0 to I 1 bc PH »26.31 to 2690.7 a 4 BF 488-01 100 + 200 + 30 0 a! 1 bc 47.5 to I 2816J7 'to BF 488-01 200 + 400 + 600 a! o c MMa gltfoSWíla 6 FROWNCIDE 500 0 to 1 1 bc 42.5 to L1C-2327.8] a ——— * ^ trtssear— I 2877 * 8 to 7 SUMILEX 500 WP 500 0 a j 1 bc 40.0 a LSD (P = .05) 0.6 | 1,2 | 53.06! 843,317 CV 295.8; 70.911 78.741 21.93

Example 23: Effectiveness of BF 488 in the control of Puccinia sorghi and

Colletotrichum graminicola corn crop, São Desidério / BA, 2009/2010 crop:

The present work was carried out to evaluate the effectiveness of

ducts in the control of common rust and anthracnose in maize crop. This was installed on corn DKB 390 YG. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil at 0.5% v / v dose was added

in all treatments except PrioriXtra treatments, where Nimbus 0.5% v / v was added. The product BF 488 (tebuconazole 100 + car- 10

15

20

25

200 + azoxystrobin 30) and BF 489 (flutriafol 62,5 + carbendazim 200 + azoxystrobin 30) are effective in controlling Puccinia sorghi and Colletotrichum graminicola (Table 17), providing excellent residual control, which can be observed by the smallest area below the disease progress curve. The efficacy of BF 488 and BF 489 is consistent, reducing disease throughout the corn plant canopy. Disease severity was high, as can be seen in the untreated control by the high area below the disease progress curve, which reflected in lower productivity.

Table 17 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in the control of Puccinia sorghi and Colletotrichum graminicola maize crop, São Desidério - BA.

ProductY

BF 488-01

PRIORI XTRA

OPERA

ProductZ

ProduetT

BF 489-03

100 + 200 + 30

120 + 240 + 36

60 + 24

LSD (P = .05j

«,!» ■ «(; (, (JUW1J. U \ Ji-i-j \ J CU:: 1U3Ü, 9 U OOip U

^^ Idjps ^ ï) ã ^ I, .pwçgisd ^ li ~ 5252'5 <'

1614.4b_ 1102.2 b 661.3 b and 1126.0 b 874 3 b

1724.2 b 1178.8 bj. J 677.0 b '1176.6 b 933.5 b

62.5 + 200 + 30 '1260.6 and 93.2 Cd 609.5 cd- t t 934.4 C 733.8 c

99.8 + 37.5

CV

185.14j 8.32]

130.95i 8.521

48.641 _ 96.96l 5.35l6.23 |

106.49 8.92

13.07

* Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra1 treatments where Nimbus 0.5% v / v was added.

Example 24: Effectiveness of BF 488 in the control of Physophella zee in maize crop, Goiânia / GO, 2009/2010 crop.

The present work was performed to evaluate the efficacy of the products in the control of tropical rust in corn crop. This was installed on corn DKB 390 YG. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) is effective in controlling Physopella zeae (Table 18 below), providing excellent residual control, which can be observed by the smaller area below the disease progress curve. The effectiveness of BF 488 is consistent, reducing disease throughout the corn plant canopy. The severity of the disease was high, as can be seen in the untreated witness due to the high area below the disease progress curve, which reflected in lower productivity.

Table 18 - Area below the disease progress curve (AUDPC) as a function of fungicide interactions in Physopella zeae control in corn crop, Goiânia - GO._

! β Eibíish ^ PMP ^ I IRifile IfIpQVLOW! SvroT. ■ 1 UNTR EATED m & Mrnla mm m4 <m 2 BF 488-01! 100 + 200 + 30 985.5 d 495.6 d, 396.9 bcd 626.0 Cd 3 BF 488-01 120 + 240 + 36 929.1 of 492.8 d 377.9 Cd 599.9 d 4 PRIORI XTRA 60 + 24 Iille m SSBaie OPERA 99.8 + 37.5 897.5 and 483.8 d - -338.5'd! A 573.3 df LSD (P = .05) 79.91 58.061 57.1 ! 510.08 CV 8.95 8.97 | 5.61 46.68

* Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added.

Example 25: Effectiveness of BF 488 in the control of Colletotrichum graminicola in maize crop, Goiânia / GO, 2009/2010 crop.

The present work was carried out to evaluate the efficacy of products in the control of anthracnose in maize crop. This was installed on corn DKB 390 YG. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. BF 488 (Tebuconazole 100 + carbendazim 200 + azoxystrobin 30) is effective in controlling Colletotrichum graminicola (Table 19 below), providing excellent residual control, which can be observed by the smallest area below the disease progress curve. . The efficacy of BF 488 is consistent, reducing disease throughout the corn plant canopy. Disease severity was high, as can be seen in the untreated control by the high area below the disease progress curve which reflected in lower productivity. BF 488 is effective in controlling C. graminicola.

Table 19 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Colletotrichum graminicola control in maize crop, Goiânia - GO.

* Assist mineral oil (emulsifiable mineral oil) was added at 0.5% v / v dose in all treatments except PrioriXtra treatments, where Nimbus 0.5% v / v was added. Example 26: Effectiveness of BF 488 in the control of Puccinia polyso-

corn crop, Goiânia / GO, 2009/2010 crop.

The present work was performed to evaluate the efficacy of the products in the control of polishing rust in corn crop. This was installed on corn DKB 390 YG. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30) is effective in controlling Puccinia polysora (Table 20 below), providing excellent residual control, which can be observed by the smaller area below the disease progress curve. The effectiveness of BF 488 is consistent, reducing disease throughout the corn plant canopy. The severity of the disease was high, as can be seen in the untreated witness due to the high area below the disease progress curve, which reflected in lower productivity. Table 20 - Area below the disease progress curve

(AUDPC) and yield as a function of fungicide interactions in Puccinia polysora control in maize crop, Goiânia - GO._

? LEAMIUM? $ '' .LEAUPPe. 976.9 of 496.1 and 382.0 of 618.3 and 2 BF 488-01 100 + 200 + 30 3 BF 488-01 120 + 240 + 36 950.3 def 505.4 fg 353.4 and 603.0 c 4 PRIORI XTRA 60 + 24 ISlillStf_ OPERA 99.8 + 37.5 939 4 def IMWfF 118320 $ ei = 5569.0 c LSD (P = .05) 79.9 84.42 61.71 50 , 1 CV 5.23 9.35 9.53 4.88

* Assist mineral oil (emulsifiable mineral oil) added

at 0.5% v / v dose in all treatments except PrioriXtra1 treatments where Nimbus 0.5% v / v was added.

Example 27: Effectiveness of BF 452, BF 465, BF 489 and BF 488 in the control of Cercospora zeae-maydis, Helminthosporium maydis, Puccinia sorghi and Phaeosphaeria maydis in maize crop. Summary of three works. Santa Helena de Goiás / GO, 2009/2010 harvest.

The present work was carried out to evaluate the efficacy of the products in the control of common rust, cercoporosis, helminthsporium and phaeospharia stain in corn crop. These were installed on DKB 390 YG corn. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30), BF 489 (flutriafol 62,5 + carbendazim 200 + azoxystrobin 30), BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling Cercospora zeae maydis, Helminthosporium maydis, Puccinia sorghi and Phaeosphaeria maydis (Tables 21, 22, 23 and 24 below). that can be observed by the smallest area below the disease progress curve. The efficacy of BF 488, BF 489, BF 452 and BF 465 products are consistent, reducing disease throughout the canopy of the maize plant. The severity of the disease was high, as can be seen in the untreated control by the high area below the disease progress curve, which reflected in lower productivity. BF 488, BF 489, BF 452 and BF 465 products are effective in improving maize crop productivity.

Table 21 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Cercospora zeae-maydis control in maize crop, Santa Helena de Goiás - GO.

Treatment No. Name Rategha ^ a.i.

Cercosporazeae- LEALOW LEAMID

maydis -AUPDC LEAUPP PLATOT

1CHECK iilllIA 2 BF 465-03 62.5 + 200 + 125 176.8 b 3 ProductX SflPfI κ 4 BF 489 62.5 + 200 + 30 175.8 b BF 452-03 100 + 200 + 125 6 ProduetY 178.5 b 7 BF 488 100 + 200 + 30 186.0 b 8 PRIORIXTRA 24 + 60 213.0 b 9 OPERATES 99.8 + 37.5 UlIÜb FOUCUR 100 198.0 b CV (%) 13.07

Productivity kg ha 1

8229.2 abe 8399.3 'ab γ8370, li ab

8166.7 abe PlM - 8395, -9, ab

8354.2 abe

IllSbc

14.29

10.25 10.67

9.69

* Assist mineral oil (emulsifiable mineral oil) added

at a dose of 0.5% v / v in all treatments except PrioriXtra treatments, where 0.5% v / v of Nimbus was added.

Table 22 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Helminthosporium maydis control in maize crop, Santa Helena de Goiás -GO.

Treatment

No. Name

1 CHECK

2 BF 465-03

3 ProductX

4 BF 489

BF 452-03

6 ProductY

7 BF 488

8 PRIORI XTRA

9 OPERA FQLICUR

CV (%)

Rategha 'a.i.

62.5 + 200 + 125

62.5 + 200 + 30 100 + 200 + 125

100 + 200 + 30

24 + 60 99.8 + 37.5 100

Helminthosporium LEALOW LEAMID

maydis - AUPDC LEAUPP PLATOT

8.09

5.77

17.99 3.95 * Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where 0.5% v / v of Nimbus

Table 23 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Puccinia sorghi control in maize crop, Santa Helena de Goiás - GO.

Treatment

Pucciriia sorghi - AUPDC

No. Name

1 CHECK

2 BF 465-03

3 ProductX

4 BF 489

BF 452-03

6 ProductY

7 BF 488

8 PRIORI XTRA

9 OPERA FOLICU R

Rateg ha "1 a.i. LEALOW LEAMID LEAUPP PLATOT

62.5 + 200 + 125

62.5 + 200f30 100 + 200 + 125

100 + 200 + 30

24 + 60 99.8 + 37.5 100

2553 bc U 169.5 cde * 241.5 bcd 182.5 cde \

Ilcle

CV (%)

14.24

18.98

18.24 13.75

* Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where 0.5% v / v Nimbus was added.

Table 24 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Phaeosphaeria maydis control in maize crop, Santa Helena de Goiás - GO.

Treatment No. Name Rate gha_1a.i.

Phaeosphaeria maydis - AUPDC LEALOW LEAMID LEAUPP PLATOT

1 CHECK

2 BF 465-03

3 ProductX

4 BF 489

BF 452-03

6 ProductY

7 BF 488

8 PRIORIXTRA

9 OPERA

FOUCUR

62.5 + 200 + 125

62.5 + 200 + 30 100 + 200 + 125

100 + 200 + 30

24 + 60 99.8 + 37.5 100

424.0 b 417, 3 b 404.8 b

mmb

402.3 b 408.0 b

MB

406.5 b

287.5 b 312.3 b

I b

290.3 b

tÉSISKWS

J2 »b

CV {%)

12.56

10.01

134.3 b

mb

146.0 b 273.5 b

136.0 b 272.3 b

12.06 8.93 * Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where 0.5% v / v of Nimbus

Example 28: Effectiveness of BF 452, BF 465, BF 489 and BF 488 in the control of Colletotrichum graminicola in maize crop, Luis Eduardo Magalhães / ΒΑ, 2009/2010 crop.

The present work was carried out to evaluate the efficacy of products in the control of anthracnose in maize crop. These were installed on DKB 390 YG corn. The beginning of the applications was preventive in the 5th leaf, being the second application pre-combing the corn. Assist emulsifiable mineral oil was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where Nimbus 0.5% v / v was added. Product BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30), product BF 489 (flutriafol 62,5 + carbendazim 200 + azoxystrobin 30), product BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling Colletotrichum graminicol (Table 25 below), providing excellent residual control that can be observed by the smallest area below the progress curve. of the disease. The efficacy of the BF 488, BF 489, BF 452 and BF 465 products are consistent, reducing disease throughout the corn plant canopy. Disease severity was high, as can be seen in the untreated control by the high area below the disease progress curve, which reflected in lower productivity. BF 488, BF 489, BF 452 and BF 465 products are effective in improving maize crop productivity.

Table 25 - Area below the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Colletotrichum graminicola control in maize crop, Luis Eduardo Maga- Ihães-BA. Treatment

10

15

20

No. Name

Rateg ha "1 a.

1 CHECK

2 BF 465-03

3 ProductX

4 BF 489

BF 452-03

6 ProductY

7 BF 488

8 PRIORIXTRA

9 OPERA FOUCUR

Colletotrichum graminicola -AUPDC LEALOW LEAMID LEAUPP

Productivity

62.5 + 200 + 125

62.5 + 200 + 30 100 + 200 + 125

100 + 200 + 30

24 + 60 99.8 + 37.5 100

68.3 b

kg ha

-1

342.8 b -430.0, b

42JIB

«SmtsgM,

80.3 b

E "" HRiSSRwi

U &9.6; b 66.5 b, 89.3 ab

tT, 8861/11, ab '9157.41 ab-iT .. 9476.85'a R 9342.59] to 8425793 ab 9189.82 ab! a

CV (%)

9.69

37.31

43.1

10.81

* Assist mineral oil (emulsifiable mineral oil) was added at a dose of 0.5% v / v in all treatments except PrioriXtra treatments where 0.5% v / v Nimbus was added.

Example 29: Summary of 3 trials to evaluate the effectiveness of BF 488 in the control of Spots in irrigated rice crop, Pelotas / RS, 2009/2010 crop.

The present work was carried out to evaluate the efficacy of the products in controlling leaf spots in the irrigated rice crop. The beginning of the applications was preventive during the rice rubber stage, with 1 to 5% of panicles emitted, with the second application occurring 15 days after the first and the third 15 days after the second application. The product BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30), the product BF 489 (flutriafol 62,5 + carbendazim 200 + azoxystrobin 30), the product BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling leaf spot (Table 26), providing excellent residual control that can be observed by the smallest area below the yield curve. disease progress. The efficacy of BF 488, BF 489, BF 452 and BF 465 is consistent, reducing disease throughout the rice plant canopy. Disease severity was high as can be seen in the untreated control by the high area below the disease progress curve, which reflected in lower productivity. Table 26 - Area below the disease progress curve (AUDPC) - summary of 3 trials evaluating the efficacy of BF 488 in controlling

Trolley of Manchas in the irrigated rice crop, Pelotas - RS.

Treatments Doses / ha G Formulated Al AUDPC 9 BRIO 187.5 0.75 29 f 3 BF 375-01 + Derosal + Stroby + OM 62.5 + 200 + 125 0.5 + 0.4 + 0.25 37 e 4 BF 489 + OM 292.5 1 40 ed BF 452-07 + OM 425 1 40 of 6 FOLICUR + Derosal + Stroby + OM 100 + 200 + 125 0.5 + 0.4 + 0.25 46 bed 8 BF 465- 05 + OM 290 0.75 46 bed 7 BF 488 + OM 330 1 47 bc 2 BF 465-05 + OM 387.5 1 50 b FOLICUR + OM 100 0.5 50 b 1 UNTR EATED 172 to LSD (P =. 05) Standard Deviation CV 7.0 2.7 1.46

Example 30: Summary of 2 trials to evaluate the effectiveness of BF 488, BF 489, BF 452 and BF 465 in the control of patches in upland rice crop, São Desidério / BA and Sinop / MT, 2009/2010 crop.

The present work was carried out to evaluate the efficacy of the products in controlling leaf spot in dry rice crop. The beginning of the applications was preventive during the rice filling stage, with 1 to 5% of panicles emitted, with the second application occurring 15 days after the first and the third 15 days after the second application. The product BF 488 (tebuconazole 100 + carbendazim 200 + azoxystrobin 30), the product BF 489 (flutriafol 62,5 + carbendazim 200 + a-zoxystrobin 30), the product BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and product BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling leaf blight - brussone (Tables 27 and 28), providing excellent residual control that can be observed at lower severity. of blast. The effectiveness of BF 488, BF 489, BF 452 and BF 465 are consistent, reducing disease throughout the rice plant canopy as well as improved panicle health. The severity of the disease was high, as can be observed in the untreated control due to its high severity, which reflected in lower productivity. BF 488, BF 489, BF 452 and BF 465 products are effective in improving rice crop productivity.

Table 27 - Severity of blast (Pyricularia grisea) - work performed to evaluate the efficacy of BF 488, BF 489, BF 452 and BF 465 in the control of patches in upland rice crop, Sinop - MT._

Trt Treatment ». No Name,

10

11

UNTREATED

BF 465-05

BF 489-03

ProductX

BF 452-07

BF 488-01

ProductY

PRIORIXTRA

BRIO + DASH

FOLICU R

OPERA

Rate PC., L / ha

Pyriculana grisea Seventy (%)

1 ProductiviW

èís & i

62.5 + 200 + 125

62.5 + 200 + 30

100 + 200 + 125

100 + 200 + 30

24 + 60

(94 + 94) + 0.25%

100

25 + 66.5

-7ΡΛ3Λ. . : .W14PA3A

6.5 b

C1P-0;

11.8 bc

Mimm *.

18.8 bcd 13.8 b 21.3 bc

Pis? -. '.' 5 '"q O.« Rtp 1 - -

3000.0 ab 2994.4 ab

Jcd Ü? *? 3,8ç_tj Jf46,3__d_ 3000,0 ab

0.3

0.75 + 0.25%

0.5

LSD (P = .05); 1.34 2.85 3.4:. ° υ 580,922 CV I 12.7 15.2 11.76 14.12

____ [* Assist 0.5% added in all treatments except treatment 8 (Nimbus 0.5% added) and treatment 9

Table 28 - Severity of blast (Pyricularia grisea) - work performed to evaluate the efficacy of BF 488, BF 489, BF 452 and BF 465 in the control of patches in upland rice crop, São Desidério - BA.

Trt -Trcatment NoSNameψ

"Addition of Assist 0.5% in all treatments except treatment 8 (Nimbus 0.5% added)

Example 31: Effectiveness of BF 489 and BF 465 in controlling Ramuraria areola in cotton crop, Luis Eduardo Magalhães / ΒΑ, 2009/2010 crop.

The experiment was performed in a randomized block design with four replications. This was carried out in Luis Eduardo Magalhães-BA, in the 2009/2010 harvest, in the cultivar Delta Opal. The onset of fungicide application 10

15

occurred at 45 days after culture emergence (DAE) with the other applications at 60, 75 and 90 DAE. High severity of ramularia occurred and the yield of the control without fungicides was 32% lower than that obtained in the best management with fungicides. BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) and BF 489 (flutriafol 62.5 + carbendazim 200 + azoxystrobim 30) are effective in controlling Ramularia areola (Table 29), providing excellent residual control that can be observed by the smallest area below the disease progress curve. The efficacy of the synergistic mixtures BF 489 and BF 465 is consistent in reducing disease throughout the cotton plant canopy and yielding productivity.

Table 29 - Area below the disease progress curve (AUDPC) as a function of fungicide interactions in Ramularea control

PRIORI PRIORI PRIORIXTRA

BF 465-05 BF 465-05 EMIN1 + PORT.

BF 465-05

BF 465-05

13 BF 489-03

IN. + PORT. PRIORICTRA EMINENT + MERTIN BF 465-05 EMIN. + PORT. BF 465-05

ATTRACT

IN. + PORT.

EMINENT + PRIORI PRIORI XTRA + PORTFRO EMINENT + PRIORI ΒΓ 465-05 BF 465-05 EMIN. + PORT.

IN. + PORT. PRIORI ΠΑ EMINENT 3F 465-05 EMIN. + PORT. BF 465-05

BF 465-05

ATTRACT

BF 465-05

LSD (P = .05)

388 388

50 + 400 84 J 50 + 240;

388! 50 + 250 388 50 + 250

50 + 50 84 + 400 50 + 50 388

313_____

50 + 250

50 + 250: 293

293 50 + 250

50 + 250'293

h ISili

■ 894 c-f; 463 c-f. 806 efg | 382; fgh 975 cde; 418 d-h 961 cd 494 cd

1001c_ [· 536 bc

. 930 cde 452 def; 763 g --- 396 e-h: 876 c 444 d-g 1119 b "! 579 b 938 cd 468 cde

20

25

Example 32: Efficacy of tebuconazole + carbendazim + azoxystrobin in controlling Ramularia areola in cotton crop. Santa Helena de Goiás / GO. 2008/2009 Harvest:

The experiment was performed in a randomized block design with four replications. This was carried out in Santa Helena de Goiás - GO on the 2008/2009 harvest in the Delta Opal cultivar. The fungicide application started at 45 days after crop emergence (DAE) with the other applications at 60, 75 and 90 DAE. The severity of ramularia was high, and the control yield without fungicides was 36.5% lower than that obtained with the best fungicide management. Product BF 452 (Tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) and Product Tebuconazole 100 + carbendazim 200 + azoxystrobin 18 are effective in controlling the Areola Rularis (Table 30), providing excellent residual control that can be observed by the smallest area below the disease progress curve. The efficacy of synergistic mixtures BF 452 and tebuconazole + carbendazim + azoxystrobin are consistent in reducing disease throughout the cotton canopy and improving cotton productivity.

Table 30 - Area under the disease progress curve (AUDPC) and yield as a function of fungicide interactions in Ramularea areola control in cotton crop, Santa Helena de Goiás - GO.

AUPDC Productivity

LEALOW LEAMID LEAUPP kg ha'1

Trt

No. TreatmentName

Rate a.i. g ha "1

10

15

20

25

1 Check

2 BF 452-03 100 + 2004-125

3 Follicul + Derosal + Stroby 100 + 200 + 125

4 Folicur + Derosal + Priori 100 + 200 + 18

PrioriXtra 84

6 Opera 91.5

7 Folicur 100

I2266, cd 2293.8 c

2350.3 c

F22pp? Cd

'2901.0' b

750.01c 1077.5 b

Tukey1S HSD (P = .05) CV (%) _

4233.9! to 3999.8 to

195.76 3.25

153.07 7.01

24.74 5.82

818.26 8.15

Example 33: Effectiveness of BF 452 and BF 465 in the control of Ramuraria areola in cotton crop, Luis Eduardo Magalhães / ΒΑ, 2008/2009 crop.

The experiment was performed in a randomized block design with four replications. This was carried out in Luis Eduardo Magalhães-BA in 2008/2009 on the Delta Opal cultivar. The fungicide application started at 45 days after crop emergence (DAE) with the other applications at 60, 75 and 90 DAE. High severity of ramularia occurred, and the control yield without fungicides was 36% lower than that obtained with the best fungicide management. BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) and BF 452 (tebuconazole 100 + carbendazim 200 + kresoxim-methyl 125) are effective in controlling Ramularia areola (Table 31). excellent residual control that can be observed by the smaller area below the disease progress curve. The efficacy of the BF 452 and BF 465 synergistic mixtures is consistent in reducing disease throughout the cotton plant canopy and providing productivity gains. Table 31 - Area below the disease progress curve (AUDPC) as a function of fungicide interactions in Ramularea areola control in cotton crop, Luis Eduardo Magalhães - BA.

; 1 Check Chedc Check Check I; 2 BF 465-03 Idem Idem Idem 62.5 + 2004-125 Idem Idem Idem: 3 ProductX; 4 FfutHafoI + Derosal + Priori Idem Idem 62.5 + 200 * 18 Idem Idem: 5 BF 452-03 Idem Idem Idem 100 + 200 + · 125 Idem Idem Idem 6 ProductY 7 ProductZ \! δ PrioriXtra Idem Idem 84 Idem Idem i Id 9 Opera Idem Idem Idem 91.5 Idem Idem J Folicur Idem Idem 100 Idem Jdem Idem I • 11 Priori Eminent + Derosal Eminent + Priori Eminent + Derosal 75 50 + 400 50 + 50 50 + 400 '12 Priori PrioriXtra PrioriXtra + DerosaI PrioriXtra 75 84 84 +400 84 ^ 13 PrioriXtra Eminent + Mertin Priori + Eminent Eminent 84 50 + 240 50 + 50 SO

2878.3 ab 3137.3ab 28536ab 2845 4 ab

Tukey1S HSO (P = .Q5)

CV (%)

Example 34: Efficacy of BF 452, BF 465 and Tebuconazole +

bendazim + azoxystrobin in the control of Colletotrichum gossypii var. cepha- Iosporioides in cotton culture, Santa Helena de Goiás / GO, 2008/2009 crop.

The experiment was performed in a randomized block design with four replications. This was carried out in Santa Helena do Goiás-GO in the 2008/2009 crop in the cultivar BRS Ipê. Fungicide application started 45 days after culture emergence (DAE) and two days after inoculation with a spore suspension at a concentration of 105 conidia / mL. The other applications occurred at 60, 75 and 90 DAE. Pulp severity was high and the control yield without fungicides 58.2% lower than that obtained in the best managements. Product BF 465 (Flutriafol 62.5 + carbendazim 200 + kresoxim-methyl 125) Product BF 452 (te-buconazole 100 + carbendazim 200 + kresoxim-methyl 125) and Product tebuconazole 100 + carbendazim 200 + azoxystrobin 18 are effective in controlling Colletotrichum gossypii var. cephalosporioides (Table 32), providing excellent residual control that can be observed by the lower disease grade throughout the evaluations. The efficacy of synergistic mixtures BF 452, BF 465 and tebuconazole + carbendazim + azoxystrobin are consistent in reducing the disease in cotton plants which reflected in maintaining crop yield.

Table 32- Severity score (scale 1 to 5) and productivity as a function of fungicide interactions in the control of Colletotrichum gossypii var. cephalosporioides in cotton culture, Santa Helena de Goi-

10

15

20

ace - GO.

Example 35: Effectiveness of BF 465 and BF 489 in the control of Collettrichum gossypii var. cephalosporioides in cotton culture, Santa Helena de Goiás / GO, 2009/2010 crop.

The experiment was performed in a randomized block design with four replications. This was carried out in Santa Helena do Goiás-GO in the 2009/2010 crop in the cultivar BRS Ipê. Fungicide application started 45 days after culture emergence (DAE) and two days after inoculation with a spore suspension at a concentration of 105 conidia / mL. The other applications occurred at 60, 75 and 90 DAE. Pulp severity was high in the experiment. The product BF 465 (flutriafol 62,5 + carbendazim 200 + kresoxim-methyl 125) and BF 489 (flutriafol 62,5 + carbendazim 200 + azoxystrobim 30) are effective in controlling Colletotrichum gossypii var. cephalosporioides (Table 33), providing excellent residual control that can be observed by the lower disease grade throughout the evaluations. The efficacy of synergistic mixtures BF 489 and BF 465 are consistent in reducing the disease in cotton plants which reflected in the maintenance of crop yield.

Table 33- Severity score (scale 1 to 5) and productivity as a function of fungicide interactions in the control of Colletotrichum gossypii var. cephalosporioides in cotton culture, Santa Helena de Goias - GO. JrtSCTreat ^ ntNOTeyAtficatiMtime?

feür1 * "* *

1 _2 _3 _4 5

and

7th

BF 465-05 EMIN. + PORT.

BF 489-03

EMIN. + PORT.

EMINENT + MERTIN

EMIN. + PORT. BF 465-05

EMIN. + PORT. BF 489-03

s- ^ JpWL ,,

SDAÍjy60bAEp5QÃ.f.feai6 '

EMINENT + PRIORI

EMINENT + PORTER

PRIORIXTRA + PRIORIXTRA PORTER

EMINENT + PRIORI

BF 465-05 EMIN. + PORT.

EMIN. + PORT. BF 465-05

BF 489-03 EMIN. + PORT.

EMIN. + PORT. BF 489-03

75 50 +400

84 50 + 240

388 75 + 325 75 + 325: 388

293 75 + 325 75 + 325! 293

293 75 + 325

75 + 325 '

293 e Xi bc 1.5 b

500 1.4 bc Li 1.4 b

00 Ι, δΊΠ ^^ Γ

Iit

b 96.6 ab g't5'b_ 95.3 ab

1.1 b 96 ^ 4 ab

LSD (P = .05)

0.6

0.54

0.53

_8.22_

26.46! 24.31; 29.78

6.02

Example 36: Summary of Two Papers - Effectiveness of BF 465 and

BF 452 on Coffee Rust (Hemileia vastatrix) control in crop

of coffee, Espirito Santo do Pinhal / SP, 2009/2010 harvest.

The tests were carried out in a block design using

case with four repetitions. These were held in Espirito Santo do Pi-

nhal-SP in the 2009/2010 harvest. The severity of rust was high in the

performance. The product BF 465 (flutriafol 62.5 + carbendazim 200 + kresoxim)

125) and BF 452 (tebuconazole 100 + carbendazim 200 + kresoximol)

125) are effective in controlling coffee rust (Table 34) and

ascochyta stain (Table 35), providing excellent residual control

which can be observed by the lower severity of the disease, smaller area

below the disease progress curve and less crop defoliation. The effi-

synergistic mixtures BF 452 and BF 465 are consistent in reducing the

disease in coffee plants which reflected in the maintenance of crop productivity.

Table 34 - Summary of two studies - Effectiveness of BF 465 and BF 452 on coffee rust control (Hemileia vastatrix) in coffee crop, Espirito Santo do Pinhal / SP. Dose_% Severity

Treatments gia / ha ml / ha 15 DAT 30 DAT 45 DAT 60 DAT AUDPC% Defoliation 1. Witness 13 33 41 47.5 1649 59.38 2.BF452-03 850 2 0 0 0.94 2 33.06 1.63 3.BF452-03 1060 2.5 0 0 0.38 1.63 20.69 1.63 4.BF452-03 1280 3 0 0 0.38 0.75 12.75 1/5 5.BF465-05 775 2 0 0 0.38 3 34.88 1.75 6.BF465-05 970 2.5 0 0 0.38 1.13 17.06 1/5 7.BF465-05 1160 3 0 0 0 1.13 10 , 69 1 8.0 Opera + Cantus 275 + 75 1.5 + 0.15 0 0 0.38 1.19 16.53 1.63 9. Operates 275 1/5 0 0 0.56 1.19 19.34 1 , 75 10. Impact 125 188 1.5 0 0 0.81 1.81 29.03 1.88

Table 35 - Summary of two studies - Effectiveness of BF 465 and BF 452 in controlling ascochyta stain in coffee crop, Espirito Santo do Pinhal / SP.

Dose

Treatments gia / ha ml / ha% Severity l.Witness 18.75 2.BF452-03 850 2 3.13 3.BF452-03 1060 2.5 2 4.BF452-03 1280 3 1.38 5.BF465-05 775 2 4 6.BF465-05 970 2.5 1.5 7.BF465-05 1160 3 1.75 8.0pera + Cantus 275 + 75 1.5 + 0.15 2.25 9. Op was 275 1.5 1.88 10. Impact 125 188 1.5 2.38

Claims (29)

Synergistic agrochemical formulation, characterized in that it comprises: (i) triazole; (ii) strobirulin; (iii) benzimidazole; and (iv) suitable adjuvants, vehicles and / or excipients; wherein the ratio of (i) :( ii) :( iii) in the formulation is about 4-15: 1-18: 10-30, preferably about 6.25-10: 3-12.5: 20 . Formulation according to claim 1, characterized in that said triazole (i) is selected from the group consisting of tebuconazole, flutriafol and mixtures thereof. Formulation according to claim 1, characterized in that said strobilurin (ii) is selected from the group consisting of azoxystrobin, kresoxim-methyl and mixtures thereof. Formulation according to claim 1, characterized in that said benzimidazole (iii) is carbendazim. Formulation according to any one of claims 1 to 4, characterized in that it comprises: - about 25 to about 250g / l triazole (i), preferably about 63 to about 100g / l; about 5 to about 500g / l strobilurin (ii), preferably about 30 to about 125g / l; and about 50 to about 500g / l benzimidazole (iii), preferably about 200g / l. Formulation according to any one of claims 1 to 5, characterized in that it comprises a total of about 10 to about 700g / l of (i), (ii) and (iii). Formulation according to any one of claims 1 to 6, characterized in that suitable adjuvants, vehicles and / or excipients are selected from the group consisting of: water, surfactants, defoaming agents, rheology, dispersing agents, humidifiers, bactericidal or bacteriostatic agents, anti-caking agents, stabilizers, antifreeze agents, clays, silicates, silicas, resins, waxes, cellulosic fibers, fertilizers, C1-C14 alcohols, glycols, acetates, ketones, glycerides saturated or unsaturated hydrocarbons, fatty acid esters, vegetable oils, mineral oils, and mixtures thereof. Formulation according to any one of claims 1 to 7, characterized in that it is in solid or liquid form, preferably the solid form is a wet powder (PM) and / or granules dispersed in water ( WDG) and the liquid form is an emulsified concentrate (EC), concentrated suspension (SC), emulsion suspension (SE) and / or concentrated emulsion (EW). Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) _ [concentration (g / 1 OOmL)] <table> table see original document page 71 </column> </ row > <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) concentration (g / 1 OOmL)] <table> table see original document page 72 </column> </row> < table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) [concentration (q / 1 OOmL)] <table> table see original document page 72 </column> </row> <table> (components) _ [concentration (g / 100mL) 1 <table> table see original document page 73 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) 'concentration (g / 1 OOmL)] <table> table see original document page 73 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) [concentration (q / 100mL) l <table> table see original document page 73 </column> </row> < table> <table> table see original document page 74 </column> </row> <table> Formulation according to wed 1 to 8, characterized in that it comprises (components) any of the claims ie: concentration (g / 1 OOmL)] <table> table see original document page 74 </column> </ row > <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) [concentration (q / 1 ORnL)! <table> table see original document page 74 </column> </row> <table> (components) _ [concentration (g / 1 OOmL)] <table> table see original document page 75 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) concentration (g / 1 OOmL)] <table> table see original document page 75 </column> </row> < table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) concentration (g / 1 OOmL)] <table> table see original document page 75 </column> </row> < table> (components) concentration (g / 1 OOmL)] <table> table see original document page 76 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: <table> table see original document page 76 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: <table> table see original document page 76 </column> </row> <table> <table> table see original document page 77 </column></row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) - [concentration (g / 100mL) <table> table see original document page 77 </column> </row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: - (components) - [concentration (g / 1 OmL)] <table> table see original document page 77 </column> </ row> <table> Formulation according to any one of claims 1 to 8, characterized in that it comprises: (components) _ [concentration (g / 1 OOmL)] _ <table> table see original document page 78 </column> </ row> <table> Process for preparing the synergistic agrochemical formulation as defined in any one of claims 1-22, characterized in that it comprises the steps of: a. mixing in a container suitable adjuvants, vehicles and / or excipients; B. add to the above mixture "a" under stirring triazole (i), strobilurin (ii) and benzimidazole (iii) in a ratio of about 4-15: 1-18: 10-30, respectively. ; ç. obtain the synergistic agrochemical formulation; and d. pack it in commercial packaging. 24. Use of triazole, strobilurin and benzimidazole, characterized in that it is in the preparation of synergistic agrochemical formulation for the control and / or control of pests and diseases caused by them in plant crops. Use of the synergistic agrochemical formulation as defined in any one of claims 1 to 22, or obtained by the process as defined in claim 23, characterized in that it is in the control and / or control of pests and diseases caused by them. in vegetable crops. Use according to any one of claims 24 and 25, characterized in that the formulation is employed in a single, sequential or simultaneous application. A method of controlling and / or controlling disease in plant crops, comprising the application of the synergistic agrochemical formulation as defined in any one of claims 1 to 22, or obtained by the process as defined. claim 23, on their habitats and / or plant crops. A method according to claim 27, characterized in that the crops are selected from soybean, cotton, maize, beans, wheat, rice, potatoes, tomatoes, citrus and tobacco, and diseases are selected. from Alternaria citri, Alternaria solani, Alternaria sp. truncata, Colletotrichum gloeosporioides, Colletotrichum gossypii var. cephalosporioides, Colletotrichum graminicola, Colletotrichum Iindemuthia- num, Corynespora cassiicola, Diaporthe phaseolorum f.sp. meridionalis, Dipplodia macrospora, Drechslera tritici-repentis, Elsinoe fawcetti, Erysiphe dif- ferium, Erysipheus, Fusile- hemi-theraum, Erysiphe diffuse a roridum, Peronospora manshurica, Phaeoisariopsis griseola, Phaeo-phaeria maydis, Phakopsora gossypii, Phakopsora meibomiae, Phakopsora pachyrhizi, Phoma spp., Phomopsis spp., Phyllosticta sojicola , Puccinia triticina, Pyricularia grisea, Ramularia areola, Rhizoctonia solani, S. macrospora, Sclerotinia sclerotiorum, Septoria glycines, Septoria Iciscici, Septoria tritici, Stenocarpella maydis, and Uromyces appendiculatus. A method according to claim 27 or 28, characterized in that the formulation is employed in a single, sequential or simultaneous application.