CN100401889C - Fungicide composition - Google Patents

Abstract

A bactericide composition is characterized by comprising a combination of at least one (systemic, protective and/or soil) bactericide in a methoxy methyl acrylate compound (I) and a compound (II). Compound (I) structural formula: the compound (II) is more than one compound selected from systemic, protective and soil bactericide. The composition has the functions of protecting, treating and eradicating plant diseases, has special effects on preventing and treating various plant diseases caused by ascomycetes, flagellates and adelomycetes, such as powdery mildew, downy mildew, anthracnose and the like, and has the advantages of quick drug action, reduced resistance, broad-spectrum activity and the like.

Description

Fungicide composition

1. Technical field

The invention relates to a fungicide composition, in particular to a composition containing a methyl methoxyacrylate compound dikestrobin and certain systemic, contact and soil fungicides and uses thereof.

Many different types of fungicides are known for use in agriculture and horticulture. However, there are many types of plant diseases to be prevented. In the actual agricultural production application, it is often very difficult to choose an ideal sterilization method for the prevention and control of various plant diseases, especially when the cultivation methods of some crops are currently changed, such as Vegetables, melons, flowers, etc. are generally planted in greenhouses. The high temperature and high humidity conditions are very easy to cause the occurrence and prevalence of various diseases, causing serious economic losses. For example, powdery mildew, downy mildew and anthracnose are the main pathogenic bacteria that harm crops. However, some leading products in the past, such as triazole and amide fungicides, have doubled due to resistance, and new varieties are urgently needed to replace them. Therefore, in order to adapt to new diseases and insect pests caused by changes in agricultural forms, new fungicide compositions with high activity and broad bactericidal spectrum should be sought.

2. Background technology

The present invention solves the problems raised above. A fungicide composition invented by the present inventors is characterized in that the composition contains a methyl methoxyacrylate compound dikestrobin (development code: ZJ0712), The compound has excellent control effects on plant diseases caused by ascomycetes, flagellates and deuteromycetes. The preparation example shown by the general formula of this compound has been described in the Chinese invention patent (application number 03120882.7). The structures and names of the compounds are as follows:

(E) Methyl 2-[2-(2,5-Dimethylphenoxymethyl)-phenyl]-3-methoxyacrylate (Phenylestrobin)

3. Contents of the invention

The object of the present invention is to provide a kind of above-mentioned composition containing methyl methoxyacrylate compound and known other bactericides to mix, and its purpose is to further improve bactericidal effect, expand prevention and control object and delay compound ( I) service life.

In the methyl methoxyacrylate compound (hereinafter referred to as compound (I)) shown in general formula (I) and select more than one fungicide among some systemic, protection and/or soil fungicides ( Hereinafter referred to as compound (II)).

It has been found through research that the bactericidal activity of compound (I) is significantly improved after being mixed with certain systemic fungicides, protective fungicides and soil fungicides.

The fungicide composition of the present invention is characterized in that the composition contains a compound (I) and at least one (systemic, protective and/or soil) fungicide in combination with the compound (II). These fungicides are available in the market, and they can be found in the "Handbook of Foreign Pesticide Varieties" compiled by the Pesticide Information Center of the Ministry of Chemical Industry or "The Pesticide Handbook" published by the British Crop Protection Council (The British Crop Protection Council). Manual) 11th Edition (1997).

Examples of (systemic, protective and soil) fungicides which are particularly suitable for use in the compositions of the invention are as follows:

Examples are as follows:

A. Systemic fungicides

1. S-Benzyl-O, O-diethyl phosphorothioate (Daobenjing)

2. S-Benzyl-O, O-Diisopropyl Phosphorothioate (Isomidanet)

3. N-(3,5-dichlorophenyl)-1,2-dimethylcyclopropane-1,2-diamide (procymidone)

4.4,4'-(O-Phenyl)bis(3-Dimethyl Thiophanate)(Methylthiophanate)

5. Methyl benzimidazol-2-ylcarbamate (carbendazim)

6. (E)-(RS)-1-(2,4-dichlorophenyl)-4,4-dimethyl-(1H-1,2,4-triazol-1-yl)pentyl-1 -en-3-ol (diniconazole)

7.2-(4-Chlorophenyl)-2-(1H-1,2,4-triazol-1-ylmethyl)capronitrile (myclobutanil)

8. 1-(4-chlorophenoxy)-3,3-dimethyl-1-(1H-1,2,4-triazol-1-yl)butanone (triazolone)

9. (RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol ( tebuconazole)

10. (±)-1[2-(2,4-dichlorophenyl)-4-propanyl, 3-dioxolan-2-ylmethyl]-1H-1,2,4-triazole (Propiconazole)

11. (RS)-2-(2,4-dichlorophenyl)-1-(1H-1,2,4-triazol-1-yl)-hexan-2-ol (hexaconazole)

12. Bis(4-fluorophenyl)methyl(1H-,2,4-triazol-1-ylmethylene)silane (fluorosilazole)

13. (5RS, 6RS)-6-Hydroxy-2,2,7,7-tetramethyl(1,2,4-triazol-1-yl)octanone-3 (octazolone).

14. Methyl 1-n-butylcarbamoyl-2-benzimidazole carbamate (benomyl)

15. N-(2-methoxyacetyl)-N-(2,6-xylyl)-D,L-alanine methyl ester (metalaxyl)

16. Ethyl hydrogen phosphate (ethyl phosphorus aluminum)

17. 4-[3-(4-chlorophenyl)-3-(3,4-dimethoxyphenyl)acryloyl]morpholine (dimethoxyphenyl)

18. 1-(2-cyano-2-methoxyiminoacetyl)-3-ethylurea (cymoxanil)

19. N-phenylacetyl-N-2,6-dimethylphenyl-DL-alanine methyl ester (benalaxyl)

20.2-Methoxy-N-(2-oxo-1,3-oxazolidin-3-yl)acetyl-2′,6′-dimethylaniline (oxaxyl)

21. (±)-a-[N-(3-Chlorophenyl)cyclopropanecarboxamido]-r-butyrolactone (Esterstrobin)

22. (±)-a-2-Chloro-N-2,6-xylylacetamido-r-butyrolactone (formofuramide)

23. 3-(Dimethylamino)propyl propyl carbamate (propamocarb)

24. (RS)-4-Chloro-N-[cyano(ethoxy)methyl]benzamide (cyanoprodil)

25. (±)-α-2-Chloro-N-2,6-xylylacetamido-r-butyrolactone (formofuramide)

26. N-(2-Furoyl)-N-(2,6-Dimethylphenyl rac-aminopropionic acid methyl ester) (furalaxyl)

27. Phosphorous acid or disodium phosphite

B. Protective fungicides

1. O-2,6-dichloro-p-tolyl O, O-dimethyl phosphorothioate (methyl tolclofos)

2.3-Chloro-N-(3-chloro-5-trifluoromethyl-2-pyridyl)-α,α,α-trifluoro-2,6-dinitro-p-toluidine (fluazinamine)

3. N-Propyl-N-[2-(2,4,6-trichlorophenoxy)ethyl]imidazole-1-carboxamide (Prochlorol)

4. Tetrachloro-1,3-benzenedicarbonitrile (tetrachloroisophthalonitrile) (chlorothalonil)

5.1,2,3,6-tetrahydro N-(trichloromethylthio)phthalimide (captan)

6.4,6-dichloro-N-(2-chlorophenyl)-1,3,5-tri-2-amine (antendazim)

7. Copper oxychloride (copper king)

8. Bis(dimethyldithiocarbamate) zinc (Ziram)

9. N-isoxazol-5-ylcarbonyl-N-(2,6-xylyl)-racem-alanine methyl ester (imocarb)

10. O-2-deoxymethylamino-α-L-glucopyranosyl-(1→2)-O-5-deoxy-3-C-formyl-α-L-lysofuranosyl

11. Multi-component coordination compound of manganese ethylene bisdithiocarbamate and zinc salt (mancozeb)

12. Tetramethylthiuram disulfide (thiram, thiram)

13.1,2,3,6-tetrahydro-N-(1,1,2,2-tetrachloroethylthio)phthalimide (captafol)

14. Sulfur (sulfur, Sulfur)

15. Ethylene bis manganese dithiocarbamate (Maneb)

C. Soil fungicide

1. O-2,6-dichloro-p-tolyl O, O-dimethyl phosphorothioate (methyl tolclofos)

2. Pentachloronitrobenzene (pentachloronitrobenzene)

3. N-isoxazol-5-ylcarbonyl-N-(2,6-xylyl)-racemic-alanine methyl ester (imocarb)

4. N-Trichloromethylthio-N-dimethylaminosulfonylanilide (Difencarb)

5.3-(Dimethylamino)propyl propyl carbamate (propamocarb)

6.2-Methoxy-N-(2-oxo-1,3-oxazolidin-3-yl)acetyl-2',6'-dimethylaniline (oxaxyl)

7. S-ethyl(3-dimethylaminopropyl)thiocarbamate (amiprocarb).

8. (5RS, 6RS)-6-hydroxy-2,2,7,7-tetramethyl(1,2,4-triazol-1-yl)octanone-3 (octazolone)

The composition of the present invention is preferably methylthiobacterium, carbendazim, diniconazole, myclobutanil, tebuconazole, propiconazole, hexamethoxazole among the compound (II) group A fungicides listed above. Coniferazole, flusilazole, phosphonium aluminum, dimethomorph, metalaxyl, cymoxanil, benalaxyl, oxadaxyl, propamocarb, phosphorous acid or disodium phosphite; the compounds listed above ( II) Fluazinam, prochloraz, chlorothalonil, mancozeb and sulfur are preferred among group B fungicides; octazolone is preferred among the compound (II) group C fungicides listed above and propamocarb.

Compound (I) may also be combined with compounds of (groups A, B and C) in compound (II) in three or more components.

According to the plant diseases that the fungicide composition of the present invention may control, can enumerate as rice blast (Pyricularia eryzae), rice flax spot (Helminthosporium oryzae), cereal powdery mildew (Erysiphe graminis), wheat head blight (Fusarium graminearum) ), wheat leaf rust (P.Striiformis; P.recondita; P.hordei and P.graminis), wheat leaf blight (Septoria tritici), citrus black spot (Phoma citricarpa), citrus scab (Sphaceloma fawcetti ), citrus anthracnose (Colletrichum gloeosporioides), apple flower rot (Sclerotiniamali), apple powdery mildew (Podosphaera leucotricha), apple leaf spot (Alternaria mali), pear scab (Venturia pirina), pear powdery mildew (Phyllactinia pyri), pear rust (Gymnosporangium haraenum), peach anthracnose (Gloeosporium laeticolor), walnut branch blight (Melanconium juglandinum), peach gummosis (Dothiorella gregaria), nuclear anthracnose (Gloeosporium laeticolor) grape downy mildew (PlasmoparaViticola) , grape black pox (Sphaceloma ampelinum), grape anthracnose (Glomerella cingulata), grape powdery mildew (Uncinula necator), grape house blight (Physalospora baccae), persimmon anthracnose (Gloeosporiumkaki), cucumber downy mildew (Pseudoperonospora cubensis) , melon anthracnose (Colletotrichum lagenarium), cucumber powdery mildew (Sphaerotheca fuliginea), cucumber blight (Mycosphaerella melonis), cucumber gray mold (Botrytis cinerea), tomato early blight (Alternaria solani), tomato leaf mold ( Cladosporium fulvum) and late blight (Phytophthora infestans), cruciferous black spot (Alternaria brassicae), canola leaf spot (Ceroosporella brassicae), flax anthracnose (Cilletotrichum boehmieriae), peanut black spot (Mycosphaerella personatum), brown spot (Cercospora arachidicola), Potato late blight (Phytophthora infestans), Strawberry powdery mildew (Sphaerotheca humuli), Tobacco powdery mildew (Erysiphecichoraoearum), Anthracnose (Colletotrichum nicotianae), Beet brown spot (Cercospora beticola), Rose scab (Diplocarpon rosae), powdery mildew (Sphaerotheca pannosa), cassava brown spot (Cercosporavicosae), cassava anthracnose (Colletotrichum gloeosporioides), litchi downy mildew (Peronophthora litchic), banana leaf spot (Cercospora musae), banana anthracnose (Gloeosporium musarum), papaya anthracnose (Colletotrichum gloeosporioides; Colletotrichum capsici), mango anthracnose (Colletotrichum gloeosporioides) and other plant diseases.

The composition of the present invention has protective, therapeutic and eradicating effects on plant diseases, and can be used for various crops such as: cucumber, pumpkin, gourd, wheat, barley, strawberry, grape, tobacco, hops, peanut, potato, tomato, cabbage, Plants such as peppers, lettuce, kale, onions, citrus, bananas, papayas, mangoes, lychees, and flowers. It has special effects on the control of various plant diseases caused by ascomycetes, flagellates and deuteromycetes, such as powdery mildew, downy mildew, anthracnose, etc., and has the advantages of rapid drug action, reduced resistance and broad-spectrum activity.

In the fungicide composition of the present invention, the mixture of compound (I) and compound (II) is not particularly limited, but the amount of the active ingredient depends on the application amount when the compound is used alone, and also depends on the combination of one compound and the other. The ratio of a compound to the degree of synergistic effect on the target fungus is related to the target fungus. Wherein, compound (I) can be mixed with any one of compounds (II), and the relative ratio based on the weight of the active component is 1:1 to 100 to 20:1, but the preferred ratio is 1:20 to 10:1. Compound (I) can also be mixed with any two of compounds (II), and the relative ratio based on the weight of active components is 1:1～20:1～100 to 50:1～20:1, but preferably The ratio is 1:1~10:3~50 to 10:1~10:1.

The composition of the present invention may also comprise a carrier, the carrier may be solid or liquid, including materials that are usually gaseous but liquid after compression, the carrier may be solid or liquid, and any carrier commonly used in the preparation of insecticidal and fungicidal compositions may be used. Any carrier can be used. The active ingredient and the carrier are formulated for easy application, for example, use in plants, seeds or soil, and easy storage, transportation or handling.

The fungicide composition of the present invention is used simply by mixing compound (I) and compound (II). But usually compound (I) and compound (II) are mixed, and mixed with solid carrier, liquid carrier and/or gaseous carrier, and add surface active agent, binding agent, dispersant, stabilizer and other preparations as required Adjuvants, prepared into water-dispersible powders, suspensions, granules, dry suspensions, emulsifiable concentrates, waters, oils, fumigants, aerosols, microcapsules, etc., or compound (I) and compound (II) Formulation is carried out as above, respectively, and after diluting with water as the case may be, the respective preparations are mixed and used. The active component in the fungicide composition of the present invention is 0.5-95% of the total weight.

These formulations can be prepared by conventional methods. For example, the active substance is mixed with a liquid solvent and/or a solid carrier, together with surfactants, emulsifiers, dispersants, stabilizers, wetting agents and possibly also binders, dyes or odorants. Therefore, the present invention also includes a method for preparing the above-mentioned fungicide composition.

Examples of suitable solid carriers include natural and synthetic clays, silicates such as natural silica and diatomaceous earth; magnesium silicates such as talc; magnesium aluminum silicates such as attapulgite and vermiculite; aluminum silicates such as kaolin calcium carbonate, calcium sulphate, ammonium sulphate; synthetic hydrated silica and synthetic calcium or aluminum silicates; particularly suitable solid carriers for powders include naturally occurring rock powders such as Attapulgite, bentonite, montmorillonite, diatomaceous earth; synthetic ground minerals such as microdispersed silicic acid or alumina; suitable particulate carriers include broken and graded natural rocks such as calcite, marble, pumice, Sepiolite, dolomite and synthetic granules made from organic and inorganic powders. In addition, pellets can be prepared from organic materials such as wood chips, peanut shells, corn cob fibers, and dried tobacco rods.

Suitable liquid carriers include water: water can be used as a solvent or diluent, organic solvents can also be used as auxiliary solvents or antifreeze additives, suitable organic solvents include aromatic hydrocarbons such as toluene, xylene benzene, phenylalkylnaphthalene and chlorinated aromatic hydrocarbons ; Chlorinated aliphatic hydrocarbons, such as chlorobenzene, vinyl chloride, trichloroethane, methylene chloride, chloroform, carbon tetrachloride and polychloroethane; Aliphatic hydrocarbons, such as petroleum fractions, cyclohexane, light Mineral oil and paraffin; however, particularly suitable are polar solvents, i.e. alcohols such as isopropanol, butanol, ethylene glycol, benzyl alcohol, furfuryl alcohol, cyclohexanol, ethers and esters; ketones such as acetone, methyl ethyl ketone , methyl ethyl ketone, methyl isobutyl ketone, cyclohexanone and butyrolactone, dimethylformamide, dimethyl sulfoxide and N-methylpyrrolidone. Mixtures of different liquids are often suitable.

The surfactant can be an emulsifying agent, a dispersing agent or a wetting agent, and it can be an ionic surfactant or a nonionic surfactant. Such as: sodium or calcium salts of polyacrylic acid and lignosulfonic acid; fatty acids or fatty amines or amides with at least 12 carbon atoms in the molecule; condensates of ethylene oxide and/or propylene oxide; glycol, sorbic acid Alcohol, sucrose or pentaerythritol fatty acid esters and their condensation products with ethylene oxide and or propylene oxide; glycerol, sorbitol, sucrose or pentaerythritol fatty acid esters; sulfate or sulfonate salts of these condensation products; in Alkali metal or alkaline earth metal salts, preferably sodium salts, of sulfuric acid or sulfonic acid esters containing at least 10 carbon atoms in the molecule, for example sodium lauryl sulfate, sodium secondary alkyl sulfate, sodium salt of sulfonated castor oil, and sodium alkaryl sulfonates, such as sodium dedecylbenzene sulfonate; and polymers of ethylene oxide, copolymers of ethylene oxide and propylene oxide. In particular the following may be used as emulsifiers and/or wetting agents; non-ionic emulsifiers such as polyoxyethylene fatty acid esters, polyoxyethylene fatty alcohol ethers, polyoxyethylene fatty amines, ethoxylated castor oil and anionic emulsifiers wait.

As binders or dispersants, such as casein, gelatin, polysaccharides (starch, arabic resin, fiber derivatives, etc.), lignin derivatives, bentonite, sugars, synthetic water-soluble polymers, etc., as stabilizers, Such as acidic isopropyl phosphate vegetable oil, mineral oil, fatty acid, etc.

According to the type, usually in the solid preparation, it contains 5-85% by weight of the active substance, preferably 20-80%; in the solution preparation, it contains 10-50% by weight of the active substance, in the suspension In the preparation, 10-60% active substance by weight.

The composition of the present invention can be formulated into: wettable powder, soluble powder, powder, (water dispersible) granule, emulsifiable concentrate, suspension, water suspension, microemulsion, water emulsion, dust, sustained release agent, aerosol or aerosol etc. Wettable powders usually contain 10-85% active ingredient, in addition to solid inert carrier, usually contain 3-10% dispersant, if necessary contain 0-10% stabilizer and/or other additives, such as penetrating agent or Adhesive. Dusts are usually formulated as powder concentrates, which have a similar composition to wettable powders but do not contain dispersants, and which are diluted in the field with other solid carriers to give compositions generally containing 0.5-10% W active substance. Granules are usually made into 10-100 international standard sieve (1.676-1.152mm) granules, which can be prepared by agglomeration or soaking methods. Usually granules contain 0.5-75% active components and 0-10% W additives, such as stabilized agents, surfactants, slow release modifiers and binders. So-called dry flowable powders consist of relatively small granules containing a relatively high concentration of active ingredient. The emulsifiable concentrate usually contains 10-50% W/V active ingredient, 2-20% W/V emulsifier and 0-20 W%/V other additives in addition to solvent and co-solvent when necessary, such as Stabilizer, penetrant and corrosion inhibitor. Suspension concentrates usually need to be stirred to obtain a stable non-sedimentary flowable product, which usually contains 10-75% W of the active ingredient, 0.5-15% W of the dispersant, and 0.1-10% W of the suspending agent, such as Protective colloids and thixotropes, 0-10% W of other additives such as defoamers, corrosion inhibitors, stabilizers, penetrants and adhesives, and water or organic liquids in which the active components are substantially insoluble ; Certain organic solids or inorganic salts may be dissolved in the formulation to help prevent sedimentation or act as an antifreeze for water.

Aqueous dispersions and emulsions, such as compositions obtained by diluting with water the aqueous suspensions or dispersible granule concentrates of the present invention are also within the scope of the present invention. Such emulsions may be of the water-in-oil or oil-in-water type and may have a thick paste-like consistency.

The products described in the present invention may be in the form of preparations, ie compositions in which the substances have been mixed. However, the ingredients of the composition may also be provided separately for formulation to be mixed directly in the barrel (can) prior to use. The concentrates of the present invention are generally mixed with water to obtain the desired concentration of active substance.

The composition of the present invention may also contain other components, such as mixed with herbicides, insecticides, nematicides, protective agents, growth regulators, plant nutrients or soil conditioners.

The protective activity of the compositions according to the invention against plant diseases caused by Ascomycetes, Flagellates and Deuteromycetes has a long duration. And it is absorbed and conducted in the plant, and slowly releases the carrier of the fungicidal compound. This slow-release formulation can be delivered to the soil near the vines and roots of the plant; or contains an adhesive component so that it can be applied directly on the vines. Therefore, the plant diseases caused by fungi can be more effectively controlled.

The compositions of the present invention can be applied by conventional methods, such as pouring, spraying, spraying, dusting, spreading or fuming. The usage amount of the fungicide composition of the present invention varies depending on the type of active ingredient compound, mixing ratio, weather conditions, preparation form, use period, method, place, control object and crops. It is very important that the application time is usually before or at the early stage of crop susceptibility. Because in actual production and application, people are not as intuitively aware of the initial disease of crops as they are for insect pests or weed damage. Therefore, it is very important to master timely prevention and treatment time. The length of time for the composition to protect against crop diseases is usually related to the selected single compound, and also related to many external factors.

4. Specific implementation

According to the embodiments, the present invention is specifically illustrated with the following examples: wherein the chemical name of the methyl acrylate compound (I) containing methoxy is: (E) 2-[2-(2,5-dimethylphenoxymethyl (yl)-phenyl]-methyl-3-methoxyacrylate as an example, but the examples are not limiting to the present invention.

Embodiment 1 54.55% (weight) wettable powder preparation

Carbendazim 45.5%

Compound (I) 9.1%

NNO 4%

Pull open powder 1.5%

Attapulgite Supplement to 100%

The components (all solid) are mixed together and pulverized in a pulverizer until the particle fineness reaches the standard value.

Embodiment 2 30% (weight) emulsifiable concentrate preparation

Propiconazole 27.3%

Compound (I) 2.7%

DMF 20%

Emulsifier ZH0101 10%

Xylene Supplement to 100%

According to the formula requirement, add solvent, original drug and emulsifier, mix evenly, heat and dissolve if necessary, and obtain transparent emulsifiable oil.

Embodiment 3 25% (weight) water suspension preparation

Propiconazole 22.7%

Compound (I) 2.3%

WPJ 4%

JFC 1%

Ethylene Glycol 3%

Xanthan Gum 0.1%

Deionized water replenished to 100%

According to the formula requirements, using water as the medium, add the original drug, dispersant, suspending agent and antifreeze into the batching kettle and mix evenly, after being pulverized by a ball mill or a shearing machine, grind it in a sand mill to make it suspending agent.

Embodiment 4 50.2% (weight) wettable powder preparation

Propiconazole 45.6%

Compound (I) 4.6%

M-9 5%

Morwet EMW 1%

Diatomaceous Earth Supplement to 100%

Preparation method is the same as embodiment 1

Embodiment 5 15% (weight) emulsifiable concentrate preparation

Fluazinam 13.6%

Compound (I) 1.4%

DMF 20%

Emulsifier ZH0101 10%

Xylene Supplement to 100%

Preparation method is the same as embodiment 2

Embodiment 6 20% (weight) water suspension preparation

Fluazinam 18.2%

Compound (I) 1.8%

WPS 3%

Sopa-270 1%

Xanthan Gum 0.15%

Vegetable urea 3%

Defoamer 0.2%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 7 60% (weight) wettable powder preparation

Fluazinam 58.5%

Compound (I) 1.5%

Supragil wp 2%

Supragil MNS190 3%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 1

Embodiment 8 15% (weight) emulsifiable concentrate preparation

Myclobutanil 13.6%

Compound (I) 1.4%

DMF 5%

Emulsifier ZHO203 10%

TEBU/45 Supplements to 100%

Preparation method is the same as embodiment 2

Embodiment 9 25% (weight) water suspension preparation

Hexaconazole 22.7%

Compound (I) 2.3%

Soprophor FL 3%

Igepal cc66o 2%

Propylene Glycol 4%

Xanthan Gum 0.2%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 10 Preparation of 50.2% (weight) water-dispersible granules

Hexaconazole 45.6%

Compound (I) 4.6%

Morwet D-425 7%

Morwet EFW 2%

Kaolin Clay Supplemented to 100%

Processing of water-dispersible granules According to the formula requirements, the original drug, dispersing wetting agent, disintegrant, filler, etc. are mixed evenly and crushed into wettable powder by airflow, and then an appropriate amount of water is added for granulation to obtain water-dispersible granules.

Example 11 Preparation of 20% (weight) smoke agent

Cyconazole 18.2%

Compound (I) 1.8%

Calcium Stearate 3%

Potassium nitrate 25%

Charcoal Powder Replenish to 100%

Preparation method is the same as embodiment 3

Embodiment 12 Preparation of 40.% (weight) emulsifiable concentrate

Prochloraz 39.％

Compound (I) 1%

DMF 20%

Emulsifier 10%

Xylene Supplement to 100%

Preparation method is the same as embodiment 2

Embodiment 13 40% (weight) water suspension preparation

Prochloraz 39%

Compound (I) 1%

WPJ 3%

NNO 2%

Glycerin 3%

Defoamer 0.2%

PVP 1%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 14 Preparation of 15% (weight) EC

Tebuconazole 13.6%

Compound (I) 1.4%

DMF 5%

Emulsifier ZHO203 10%

TEBU/45 Supplement to 100%%

Preparation method is the same as embodiment 2

Embodiment 15 Preparation of 10% (weight) water suspension

Tebuconazole 9.1%

Compound (I) 0.9%

M-9 1.5%

NNO 2.5%

Sopa-270 2%

Glycerin 3%

Xanthan Gum 0.3%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 16 Preparation of 60% (weight) water-dispersible granules

Tebuconazole 54.5%

Compound (I) 5.5%

Multibacterial 9%

Morwet D-42 55%

Morwet EFW 2%

Sodium Bentonite Supplement to 100%

Preparation method is the same as embodiment 10

Embodiment 17 65% (weight) wettable powder preparation

Tebuconazole 59.1%

Compound (I) 5.9%

Sodium Sulfate 8%

Sodium Lignosulfonate 5%

Sodium Lauryl Sulfate 5%

Kaolin Supplement to 100%

Preparation method is the same as embodiment 1

Embodiment 18 Preparation of 50% (weight) water-dispersible granules

Tebuconazole 35.4%

Compound (I) 4.6%

Carbendazim 10%

Morwet D-425 4%

Morwet EFW 3%

Kaolin Supplement to 100%

Preparation method is the same as embodiment 10

Embodiment 19 5% (weight) dust preparation

Tebuconazole 4.5%

Compound (I) 0.5%

Clay Filled to 100%

Preparation method is the same as embodiment 1

Embodiment 20 5% (weight) dust preparation

Fluazinam 4.9%

Compound (I) 1%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 1

Example 21 Preparation of 20% (weight) smoke agent

Tebuconazole 18.2%

Compound (I) 1.8%

Lead Sulfate Tribasic 5%

Ammonium nitrate 30%

Charcoal Powder Replenish to 100%

Preparation method is the same as embodiment 1

Embodiment 22 52.5% (weight) wettable powder preparation

Chlorothalonil 45.5%

Compound (I) 4.6%

Sodium Lauryl Sulfate 5%

Diatomaceous earth 10%

Sodium Lignosulfonate 5%

Chalk Replenish to 100%

Preparation method is the same as embodiment 1

Embodiment 23 25.76% (weight) water suspension preparation

Phosphorous acid 24.24%

Compound (I) 1.52%

Morwet D-425 3%

JFC 1.5%

Xanthan Gum 0.2%

Ethylene Glycol 3%

Defoamer 0.2%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 24 70% (weight) wettable powder preparation

Phosphate Aluminum 66.67%

Compound (I) 3.33%

AGO-300 2%

Pull open powder 1%

Kaolin Filled to 100%

Preparation method is the same as embodiment 1

Embodiment 25 Preparation of 50% (weight) water-dispersible granules

Phosphate Aluminum 47.62%

Compound (I) 2.38%

AGS-400 3%

Morwet EFW 1%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 10

Embodiment 26 Preparation of 10% (weight) water suspension

Phosphate Aluminum 9.52%

Compound (I) 0.48%

WPJ 3%

NP-10 1%

Xanthan Gum 0.2%

Propylene Glycol 4%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 27 61.47% (weight) wettable formulation preparation

Metalaxyl 58.54%

Compound (I) 2.93%

NNO 3%

M-13 2%

Pull open powder 1.5%

Diatomaceous Earth Supplement to 100%

Preparation method is the same as embodiment 1

Embodiment 28 25% (weight) water suspension preparation

Metalaxyl 23.8%

Compound (I) .19%

WPJ 3%

Morwet EFW 1%

Ethylene Glycol 4%

Xanthan Gum 0.15%

Defoamer 0.2%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 29 Preparation of 50% (weight) water-dispersible granules

Metalaxyl 47.62%

Compound (I) 2.38%

Morwet D-425 4%

Morwet EFW 2%

Ammonium Sulfate 10%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 10

Embodiment 30 Preparation of 20% (weight) smoke agent

Carbendazim 19%

Compound (I) 1%

Potassium chlorate 10%

Ammonium Nitrate 15%

Charcoal Powder Replenish to 100%

Preparation method is the same as embodiment 1

Embodiment 31 Preparation of 10% (weight) water suspension

Dimethomorph 8%

Compound (I) 2%

1601 4%

Sopa-270 2%

Bentonite 1%

Pull open powder 1%

Glycerin 3%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Example 32 Preparation of 25% (weight) wettable powder

Dimethomorph 20%

Compound (I) 5%

Sodium Lignosulfonate 4%

Sodium dodecylbenzenesulfonate 1%

Sodium Bentonite Clay Supplemented to 100%

Preparation method is the same as embodiment 1

Embodiment 33 Preparation of 15.7% (weight) emulsifiable concentrate

Benalaxyl 14.3%

Compound (I) 1.4%

DMF 30%

Emulsifier ZH0207 12%

Toluene Supplement to 100%

Preparation method is the same as embodiment 2

Embodiment 34 Preparation of 10% (weight) water suspension

Benalaxyl 9.52%

Compound (I) 4.8%

WPJ 3%

JFC 1%

Bentonite 1%

Propylene Glycol 5%

Defoamer 0.2%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 35 Preparation of 60% (weight) water-dispersible granules

Propamocarb 57.14%

Compound (I) 5.72%

Morwet D-425 5%

Morwet EFW 2%

Ammonium Sulfate 10%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 10

Embodiment 36 Preparation of 10% (weight) water suspension

Propamocarb 9.52%

Compound (I) 0.48%

WPS-6 3%

Pull open powder 1%

Silica 0.5%

Magnesium Aluminum Silicate 2%

Ethylene Glycol 4%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Embodiment 37 Preparation of 60% (weight) water dispersible granules

Propamocarb 50%

Compound (I) 1%

Phosphorus Aluminum 9%

Morwet D-425 5%

Morwet EFW 3%

Urea 5%

Kaolin Clay Supplemented to 100%

Preparation method is the same as embodiment 10

Embodiment 38 Preparation of 5% (weight) dust agent

Metalaxyl 4.76%

Compound (I) 0.24%

Bentonite Clay Supplemented to 100%

Preparation method is the same as embodiment 1

Embodiment 39 Preparation of 50% (weight) water-dispersible granules

Propamocarb 40%

Compound (I) 2%

Phosphorus Aluminum 8%

Morwet D-425 4%

Morwet EFW 3%

Ammonium Sulfate 10%

Attapulgite Supplement to 100%

Preparation method is the same as embodiment 10

Embodiment 40 Preparation of 10% (weight) water suspension

Propamocarb 8%

Compound (I) 0.5%

Phosphorus Aluminum 1.5%

WPJ 3%

1601 1%

Xanthan Gum 0.3%

Ethylene Glycol 4%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

Example 41 Preparation of 5% (weight) dust agent

Propamocarb 4.76%

Compound (I) 0.24%

Diatomaceous Earth Supplement to 100%

Preparation method is the same as embodiment 1

Example 42 Preparation of 55% (weight) wettable powder

Propamocarb 52.38%

Compound (I) 2.62%

Sodium Diisooctyl Sulfosuccinate 2%

Sodium Lauryl Sulfate 8%

Sodium Lignosulfonate 8%

Kaolin Supplement to 100%

Preparation method is the same as example 1

Example 43 Preparation of 20% (weight) smoke agent

Propamocarb 19%

Compound (I) 1%

Calcium Stearate 3%

Potassium nitrate 25%

Charcoal Powder Replenish to 100%

Preparation method is the same as embodiment 1

Example 44 Preparation of 50% (weight) wettable powder

Mancozeb 47.62%

Compound (I) 2.38%

Sodium Lignosulfonate 3%

K12 2%

Pull open powder 0.5%

Bentonite Supplement to 100%

Preparation method is the same as embodiment 1

Example 45 Preparation of 20% (weight) smoke agent

Mancozeb 19%

Compound (I) 1%

Ammonium chlorate 30%

Charcoal Powder Replenish to 100%

Preparation method is the same as embodiment 1

Embodiment 46 Preparation of 50% (weight) water-dispersible granules

Phosphate Aluminum 40%

Compound (I) 2%

Dimethomorph 8%

Morwet D-425 4%

Morwet EFW 3%

Ammonium Sulfate 15%

Kaolin Supplement to 100%

Preparation method is the same as embodiment 10

Example 47 Preparation of 52.5% (weight) wettable powder

Mancozeb 50%

Compound (I) 2.5%

Sodium Lauryl Sulfate 5%

Diatomaceous earth 10%

Sodium Lignosulfonate 5%

Chalk Replenish to 100%

Preparation method is the same as embodiment 1

Embodiment 48 Preparation of 10% (weight) water suspension

Cymoxanil 9.52%

Compound (I) 0.48%

Sodium Lauryl Sulfate 2%

Ethylene Glycol 4%

Deionized water replenished to 100%

Preparation method is the same as embodiment 3

The present invention will give 2 kinds of active ingredient compounds after mixing, carry out biological activity measurement indoors, and the expected control effect of plant diseases is obtained by the Colby formula (1) of following mathematical formula 1:

[Calculation formula 1]

$\mathrm{<span\; class="notranslate">\; E.</span>}<span\; class="notranslate">\; =</span>\mathrm{<span\; class="notranslate">\; x</span>}<span\; class="notranslate">\; +</span>\mathrm{<span\; class="notranslate">\; Y</span>}<span\; class="notranslate">\; -</span>\frac{\mathrm{<span\; class="notranslate">\; x</span>}<span\; class="notranslate">\; \&times;</span>\mathrm{<span\; class="notranslate">\; Y</span>}}{\mathrm{<span\; class="notranslate">\; 100</span>}}$

x: the actual control effect (%) when the active ingredient compound A is treated with m ppm

Y: the actual control effect (%) when the active ingredient compound B is treated with m ppm

E: Theoretical control effect (%) when active ingredient compound A is treated with m ppm and B with n ppm (hereinafter referred to as theoretical control effect).

In general, when the actual control effect (%) obtained by mixing the two active ingredient compounds on crop diseases is greater than the theoretical control effect (%), it can be said that the two active ingredient compounds are mixed. There is a synergistic effect. Use the following formula 2 to find the actual control effect (%).

[Calculation formula 2]

In the greenhouse, some fungicide compositions were tested for the control of cucumber powdery mildew and cucumber downy mildew, and the fungicidal activity between the compositions and between the compositions and a single agent was compared. The following efficacy test examples 49 to 68 show that the fungicide composition of the present invention has excellent control effects on plant diseases.

Example 49 Protection activity test of cucumber powdery mildew

In the greenhouse, the preparation of Example 8 and 10% compound (I) aqueous suspension were subjected to a comparative test of biological activity against cucumber powdery mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method: Put sandy loam in plastic pots, sow cucumber seeds, cultivate cucumber seedlings to the stage of two leaves and one core in the greenhouse, put them on the crop sprayer for foliar spray treatment, and protect the test 24 hours after spraying Inoculation of cucumber powdery mildew spores. The inoculated test materials were cultured in an artificial climate chamber for about 10 days for investigation.

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 49.0 myclobutanil 5 37.8 Mixture 0.5+5 92.5

The test results show that the control effect of compound (I) 0.5ppm plus myclobutanil 5ppm on cucumber powdery mildew is 92.5%, which is obviously higher than the control effect of two single agents.

Example 50 Cucumber Powdery Mildew Protective Activity Test

In the greenhouse, the preparation of Example 9 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber powdery mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method is identical with embodiment 49

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 49.0 Hexaconazole 5 35.5 Mixture 0.5+5 90.0

The test results show that the control effect of compound (I) 0.5ppm plus hexaconazole 5ppm on cucumber powdery mildew is 90.0%, obviously higher than the control effect of two single agents.

Example 51 Protection activity test of cucumber powdery mildew

In the greenhouse, the preparation of Example 3 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber powdery mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method is identical with embodiment 49

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 48.7 Propiconazole 5 38.2 Mixture 0.5+5 91.8

The test results show that the control effect of compound (I) 0.5ppm plus propiconazole 5ppm on cucumber powdery mildew is 91.8%, which is obviously higher than the control effect of two single agents.

Example 52 Protection activity test of cucumber powdery mildew

In the greenhouse, the preparation of Example 5 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber powdery mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method is identical with embodiment 49

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 51.0 fluazinam 20 34.8 Mixture 0.5+5 90.7

The test results show that the control effect of compound (I) 0.5ppm plus fluazinam 20ppm on cucumber powdery mildew is 90.7%, which is obviously higher than the control effect of two single agents.

Example 53 Protection activity test of cucumber powdery mildew

In the greenhouse, the preparation of Example 12 and 10% compound (I) aqueous suspension were subjected to a comparative test on the biological activity of cucumber powdery mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method is identical with embodiment 49

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 51.0 Prochloraz 20 35.3 Mixture 0.5+5 91.0

The test results show that the control effect of compound (I) 0.5ppm plus prochloraz 20ppm on cucumber powdery mildew is 91.0%, which is obviously higher than the control effects of the two single agents.

Example 54 Protection activity test of cucumber powdery mildew

In the greenhouse, compound (I), tebuconazole and carbendazim 3 single doses were put into the bucket separately and 3 single doses were mixed uniformly according to the ratio, the effect on cucumber cucumber powdery mildew (triadimefon resistant strain) The control effect was compared.

Test method is identical with embodiment 49

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 48.7 Tebuconazole 5 31.0 Carbendazim 20 0 Mixture 0.5+5+20 100

The test results show that: compound (I) 0.5ppm plus tebuconazole 5ppm plus carbendazim 20ppm tank mixed, the control effect on cucumber powdery mildew is 100%, which is obviously higher than the control effect of three single agents.

Example 55 Cucumber Powdery Mildew Protective Activity Test

Put compound (I), tebuconazole and thiophanate-methyl 3 medicaments alone and 3 medicaments into the barrel according to the ratio and mix evenly in the greenhouse. effect test.

The test method is the same as that of Test Example 1

active substance Concentration (ppm) Prevention effect (%) Compound (I) 0.5 48.7 Tebuconazole 5 31.0 thiophanate-methyl 20 0 Mixture 0.5+5+20 100

The test results show that: compound (I) 0.5ppm plus tebuconazole 5ppm plus carbendazim 20ppm tank mixed, the control effect on cucumber powdery mildew is 100%, which is obviously higher than the control effect of three single agents.

Example 56 Cucumber powdery mildew protective activity test

Each composition was subjected to a pot bioactivity assay in a greenhouse. A comparative test on the activity between the compositions, and the fungicidal activity of the composition and a single agent against cucumber powdery mildew. The results show that: each composition has obvious synergistic effect on the control of cucumber powdery mildew compared with a single agent. The following is a formulation screening study of the fungicidal composition.

Experimental crop: cucumber (Baoyang No. 5)

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Test method: use the cucumber seedling potted plant test method, put sandy loam in plastic pots, sow cucumber seeds, wait for the cucumber seedlings to grow to the stage of two leaves and one core, and put the test cucumber seedlings on the crop sprayer for foliar spraying For treatment, the treated crops were naturally dried in the shade for 24 hours and then inoculated with cucumber powdery mildew spores. After inoculation, the test materials were cultivated in the artificial climate chamber for about 10 days to investigate the incidence rate (%), and use formula 2 to obtain the actual control effect (%).

Test compound Concentration of active ingredients (ppm) Actual control effect (%) Theoretical control effect (%) Compound (I) + Tebuconazole 0.5+5 93.2 66.9 Compound (I)+hexaconazole 0.5+5 90.5 68.9 Compound (I) + Diniconazole 0.5+5 91.9 67.8 Compound (I) + carbendazim 0.5+40 88.6 69.3 Compound (I) + sulfur 0.5+50 85.3 71.4 Compound (I) + propiconazole 0.5+5 91.5 70.4 Compound (I) + prochloraz 0.5+20 87.2 68.8 Compound (I)+fluazinam 0.5+20 85.5 67.9 Compound (I) + myclobutanil 0.5+5 92.7 70.5 Compound (I) + Triadimefon 0.5+50 75.9 56.2 Compound (I) + thiophanate-methyl 0.5+40 86.9 67.9 Compound (I) 0.5 51.3 Tebuconazole 5 32.7 Hexaconazole 5 36.2 Diniconazole 5 33.8 Carbendazim 40 37.0 sulfur 50 41.3 Propiconazole 5 39.2 Prochloraz 20 35.9 Fluazinam 20 34.0 myclobutanil 5 39.5 Triadimefon 50 10.1 thiophanate-methyl 40 34.0

Example 57 Protective and therapeutic activity test on cucumber powdery mildew

In greenhouse, compound (I) is mixed with tebuconazole in a ratio barrel, and then a series concentration is established, namely 40, 20 and 10ppm, simultaneously with 25% tebuconazole emulsifiable concentrate (treatment concentration is the same as the mixture) and 10% compound (I) ) water suspension concentrate is carried out comparative test to control powdery mildew (test method is identical with embodiment 49). The results show that compound (I) and tebuconazole have obvious synergistic effect on preventing and treating powdery mildew when used in combination.

Example 58 Simulated Field Efficacy Test of Controlling Cucumber Powdery Mildew

In greenhouse, compound (I) is mixed with tebuconazole according to the ratio barrel, and the treatment concentration of mixture is 40, 20 and 10ppm and is identical with mixture with 25% tebuconazole emulsifiable concentrate treatment concentration simultaneously, carries out simulation field medicine to control powdery mildew effect test.

Experimental crop: cucumber (Baoyang No. 5)

Control object: Cucumber powdery mildew (triadimefon-resistant strain)

Cultivate potted cucumber seedlings to the 3-4 leaf stage, allow them to naturally develop to the initial stage, set a series of concentrations for the test composition, and place the test cucumber seedlings on a crop sprayer for foliar spray treatment. The treated test materials were cultured under natural conditions for 10 days, and then the control effect was investigated. The results show that: the compound (I) is mixed with tebuconazole, and the composition is used at the initial stage of cucumber powdery mildew under natural conditions, and the control effect is more than 89% at the concentration of 20-40ppm.

Example 59 Protection activity test of cucumber downy mildew

In the greenhouse, the preparation of Example 24 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber downy mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method: put sandy loam in plastic pots, sow cucumber seeds (Baoyang No. 5), and when the cucumber seedlings are cultivated to the two-leaf and one-heart stage in the greenhouse, spray the foliage with the crop sprayer, and after 24 hours Cucumber downy mildew spores were inoculated, and the inoculated test materials were cultured in an artificial climate chamber for 7 days to investigate the incidence.

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 52.6 Phosphorus aluminum 100 5.0 Mixture 5+100 85.4

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of fosmoluminum on cucumber downy mildew is 85.4%, which is obviously higher than that of the two single agents.

Embodiment 60 Cucumber downy mildew protective activity test

In the greenhouse, the preparation of Example 22 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber downy mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (1) 5 52.6 Chlorothalonil 100 21.0 Mixture 5+100 88.0

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of chlorothalonil on cucumber downy mildew is 88.0%, which is obviously higher than that of the two single agents.

Example 61 Protection activity test of cucumber downy mildew

In the greenhouse, the preparation of Example 32 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber downy mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 53.0 Cymoxanil 100 39.2 Mixture 5+100 92.7

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of cymoxanil on cucumber downy mildew is 92.7%, which is obviously higher than that of the two single agents.

Example 62 Protection activity test of cucumber downy mildew

In the greenhouse, the preparation of Example 33 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber downy mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 53.0 benalaxyl 100 30.1 Mixture 5+100 91.3

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of benalaxyl on cucumber downy mildew is 91.3%, which is obviously higher than that of the two single agents.

Example 63 Protection activity test of cucumber downy mildew

In the greenhouse, the preparation of Example 6 and 10% compound (I) aqueous suspension were subjected to a biological activity comparison test against cucumber downy mildew.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 52.0 fluazinam 100 25.9 Mixture 5+100 85.6

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of fluazinam on cucumber downy mildew is 85.6%, which is obviously higher than that of the two single agents.

Embodiment 64 Cucumber downy mildew protective activity test

In greenhouse, 3 medicaments of compound (I), propamocarb and fofosalum were put into the bucket and mixed uniformly together with 3 single agents according to the ratio, to prevent and control cucumber downy mildew (metalaxyl-resistant strain) Effectiveness comparison test.

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 50.8 propamocarb 50 0 Phosphorus aluminum 100 6.0 Mixture 5+50+100 100

The test results show that: the compound (I) 5ppm plus propamocarb 50ppm plus 100ppm fosfosal in a tank mix has a 100% control effect on cucumber downy mildew, which is obviously higher than the control effect of 3 single agents.

Example 65 Protective activity test of cucumber downy mildew

Try compound (I), propamocarb and dimethomorph alone and 3 single doses in the greenhouse and put them into the bucket according to the ratio and mix them evenly, to the control effect of cucumber downy mildew (metalaxyl resistant strain) Do a comparison test.

Test method is identical with embodiment 59

active substance Concentration (ppm) Prevention effect (%) Compound (I) 5 50.8 Dimethomorph 5 23.6 propamocarb 50 0 Mixture 5+5+50 100

The test results show that: compound (I) 5ppm plus dimethomorph 5ppm plus propamocarb 50ppm tank mixed, the control effect on cucumber downy mildew is 100%, obviously higher than the control effect of 3 single doses.

Example 66 Protective activity test of cucumber downy mildew

Each composition was subjected to a pot bioactivity assay in a greenhouse. A comparative test on the activity between the compositions, and between the composition and a single agent on the fungicidal activity of cucumber downy mildew. The results show that: each composition has a synergistic effect on the control of cucumber downy mildew compared with a single agent. The following is a formulation screening study of fungicidal composition against cucumber downy mildew.

Experimental crop: cucumber (Baoyang No. 5)

Control object: Cucumber downy mildew (metalaxyl resistant strain)

Test method: adopt the potted method of cucumber seedlings, put sandy loam in plastic pots, sow cucumber seeds, cultivate in the greenhouse until the cucumber seedlings grow to the stage of two leaves and one heart, and put the test yellow seedlings on the crop sprayer for leafing. Surface spray treatment, the treated crops were naturally dried in the shade for 24 hours and then inoculated with cucumber downy mildew spores. After the inoculated test materials were cultivated in the artificial climate chamber for 7 days, the incidence rate (%) was investigated, and the actual control value (%) was obtained by formula 2.

Test compound Concentration of active ingredients (ppm) Actual control value (%) Expected control value (%) Compound (I) + propamocarb 5+100 96.3 65.4 Compound (I) + cymoxanil 5+100 93.1 72.9 Compound (I) + Phosphorus Aluminum 5+100 89.4 56.6 Compound (I) + carbendazim 5+100 87.0 63.5 Compound (I) + chlorothalonil 5+100 85.6 65.5 Compound (I) + phosphorous acid 5+400 75.9 67.2 Compound (I) + metalaxyl 5+100 87.9 62.7 Compound (I)+fluazinam 5+100 89.3 62.6 Compound (I) + benalaxyl 5+100 94.9 57.5 Compound (I) + dimethomorph 5+10 92.8 74.7 Compound (I) + Mancozeb 5+50 91.5 69.8 Compound (I) 5 53.4 propamocarb 100 25.8 Cymoxanil 100 41.0 Phosphorus aluminum 100 6.8 Carbendazim 100 21.7 Fluazinam 100 26.0 benalaxyl 100 29.7 phosphoric acid 400 20.0 Chlorothalonil 100 19.8 Metalaxyl 100 8.7 Dimethomorph 10 45.8 Mancozeb 50 35.3

Example 67 Cucumber downy mildew protective activity test

Compound (I) and propamocarb are prepared separately earlier in the greenhouse, then 2 single doses are put into buckets in proportion and mixed, the mixture is set as a series of concentrations, and mixed with 10% compound (I) water suspension and 72.2 % propamocarb 2 single doses were tested for their biological activity against cucumber downy mildew.

Test method is identical with embodiment 59

The results show that compound (I) and propamocarb are used in combination, and the control effect on cucumber downy mildew at the same concentration is significantly better than that of propamocarb alone.

Example 68 Control efficacy test of cucumber downy mildew in field plots

The compound and 72.2% propamocarb aqueous emulsion were tested for efficacy comparison in field plots against cucumber downy mildew.

Experimental crop: cucumber (Jinchun No. 5)

Control object: Cucumber downy mildew

Compound (I) and propamocarb were mixed in barrels according to the ratio, and the treatment doses of the mixture and propamocarb were 1000, 500 and 250ga.i./hm ² respectively, and field plot efficacy tests were carried out on cucumber downy mildew . Result of the test shows: compound (I) and propamocarb are mixed, and begin medication when the onset of cucumber downy mildew to initial stage, under the dose of 1000, 500 and 250g ai/hm ² , through secondary medication, its control effect is respectively 93.0, 87.0 and 73.6%, significantly better than the control effect of propamocarb single agent. It shows that the composition of the present invention has a more obvious synergistic effect on the control of cucumber downy mildew.

Test Example 69 Protective Activity Test of Cucumber Anthracnose

In the greenhouse, the biological activity comparison test of Example 1 and 10% compound (I) aqueous suspension against cucumber anthracnose was carried out.

Cucumber Variety: Baoyang No. 5

Control object: Cucumber anthracnose

Test method: put sandy loam in plastic pots, sow cucumber seeds (Baoyang No. 5), cultivate cucumber seedlings in the greenhouse to the stage of two leaves and one heart, and put the test materials on the crop sprayer for foliar spraying. 24 hours later, the cucumber anthracnose spores were inoculated, and the inoculated test materials were cultured in an artificial climate chamber for 7 days to investigate the incidence.

active substance Concentration (ppm) Prevention effect (%) Compound (I) 10 48.5 Carbendazim 100 27.4 Mixture 10+100 91.6

The test results show that the control effect of 5 ppm of compound (I) plus 100 ppm of carbendazim on cucumber downy mildew is 91.6%, which is obviously higher than that of the two single agents.

Claims (6)

1. a bactericide composition is characterized in that comprising (E) 2-[2-(2,5-dimethylphenoxymethyl)-phenyl]-3-methoxymethyl acrylate and ( RS)-1-(4-chlorophenyl)-4,4-dimethyl-3-(1H-1,2,4-triazol-1-ylmethyl)pentan-3-ol two active groups The weight ratio of the two active components is 1:1-20. 2. bactericide composition according to claim 1 is characterized in that also containing at least one auxiliary agent in the composition, auxiliary agent is selected from dimethylformamide, emulsifier ZH0203, TEBU/45, M-9, NNO, Sopa-270, Glycerin, Xanthan Gum, Deionized Water, Morwet D-425, Morwet EFW, Sodium Bentonite, Sodium Lignosulfonate, Sodium Lauryl Sulfate, Kaolin, Pottery Clay, Lead Sulfate Tribasic , ammonium nitrate and charcoal powder. 3. The fungicide composition according to claim 1, characterized in that the composition is formulated as emulsifiable concentrate, water suspension, water dispersible granule, wettable powder, dust or smoke. 4. The fungicide composition according to claim 1, characterized in that the weight percentage of active components in the composition is 5-65%. 5. The purposes of the fungicide composition according to claim 1, for preventing and treating plant diseases caused by Ascomycetes pathogenic bacteria. 6. The fungicide composition according to claim 5, wherein the plant disease caused by the Ascomycete pathogen is cucumber powdery mildew.