BRPI1008674A2 - Métodos de redução do desenvolvimento de pragas resistentes. - Google Patents
Métodos de redução do desenvolvimento de pragas resistentes.Info
- Publication number
- BRPI1008674A2 BRPI1008674A2 BRPI1008674-9A BRPI1008674A BRPI1008674A2 BR PI1008674 A2 BRPI1008674 A2 BR PI1008674A2 BR PI1008674 A BRPI1008674 A BR PI1008674A BR PI1008674 A2 BRPI1008674 A2 BR PI1008674A2
- Authority
- BR
- Brazil
- Prior art keywords
- development
- reducing
- methods
- resistant pests
- pests
- Prior art date
Links
- 241000607479 Yersinia pestis Species 0.000 title 1
Classifications
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
- C12N15/8286—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance for insect resistance
-
- A—HUMAN NECESSITIES
- A01—AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
- A01N—PRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
- A01N25/00—Biocides, pest repellants or attractants, or plant growth regulators, characterised by their forms, or by their non-active ingredients or by their methods of application, e.g. seed treatment or sequential application; Substances for reducing the noxious effect of the active ingredients to organisms other than pests
-
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N15/00—Mutation or genetic engineering; DNA or RNA concerning genetic engineering, vectors, e.g. plasmids, or their isolation, preparation or purification; Use of hosts therefor
- C12N15/09—Recombinant DNA-technology
- C12N15/63—Introduction of foreign genetic material using vectors; Vectors; Use of hosts therefor; Regulation of expression
- C12N15/79—Vectors or expression systems specially adapted for eukaryotic hosts
- C12N15/82—Vectors or expression systems specially adapted for eukaryotic hosts for plant cells, e.g. plant artificial chromosomes (PACs)
- C12N15/8241—Phenotypically and genetically modified plants via recombinant DNA technology
- C12N15/8261—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield
- C12N15/8271—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance
- C12N15/8279—Phenotypically and genetically modified plants via recombinant DNA technology with agronomic (input) traits, e.g. crop yield for stress resistance, e.g. heavy metal resistance for biotic stress resistance, pathogen resistance, disease resistance
-
- Y—GENERAL TAGGING OF NEW TECHNOLOGICAL DEVELOPMENTS; GENERAL TAGGING OF CROSS-SECTIONAL TECHNOLOGIES SPANNING OVER SEVERAL SECTIONS OF THE IPC; TECHNICAL SUBJECTS COVERED BY FORMER USPC CROSS-REFERENCE ART COLLECTIONS [XRACs] AND DIGESTS
- Y02—TECHNOLOGIES OR APPLICATIONS FOR MITIGATION OR ADAPTATION AGAINST CLIMATE CHANGE
- Y02A—TECHNOLOGIES FOR ADAPTATION TO CLIMATE CHANGE
- Y02A40/00—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production
- Y02A40/10—Adaptation technologies in agriculture, forestry, livestock or agroalimentary production in agriculture
- Y02A40/146—Genetically Modified [GMO] plants, e.g. transgenic plants
Landscapes
- Life Sciences & Earth Sciences (AREA)
- Health & Medical Sciences (AREA)
- Genetics & Genomics (AREA)
- Engineering & Computer Science (AREA)
- Zoology (AREA)
- Wood Science & Technology (AREA)
- Biomedical Technology (AREA)
- Molecular Biology (AREA)
- Organic Chemistry (AREA)
- Biotechnology (AREA)
- General Engineering & Computer Science (AREA)
- Bioinformatics & Cheminformatics (AREA)
- General Health & Medical Sciences (AREA)
- Chemical & Material Sciences (AREA)
- Plant Pathology (AREA)
- Biochemistry (AREA)
- Cell Biology (AREA)
- Physics & Mathematics (AREA)
- Microbiology (AREA)
- Biophysics (AREA)
- Pest Control & Pesticides (AREA)
- Insects & Arthropods (AREA)
- Agronomy & Crop Science (AREA)
- Toxicology (AREA)
- Dentistry (AREA)
- Environmental Sciences (AREA)
- Agricultural Chemicals And Associated Chemicals (AREA)
- Breeding Of Plants And Reproduction By Means Of Culturing (AREA)
Applications Claiming Priority (2)
| Application Number | Priority Date | Filing Date | Title |
|---|---|---|---|
| US15368909P | 2009-02-19 | 2009-02-19 | |
| PCT/US2010/024661 WO2010096613A1 (en) | 2009-02-19 | 2010-02-19 | Blended refuge deployment via manipulation during hybrid seed production |
Publications (1)
| Publication Number | Publication Date |
|---|---|
| BRPI1008674A2 true BRPI1008674A2 (pt) | 2015-08-25 |
Family
ID=42101731
Family Applications (1)
| Application Number | Title | Priority Date | Filing Date |
|---|---|---|---|
| BRPI1008674-9A BRPI1008674A2 (pt) | 2009-02-19 | 2010-02-19 | Métodos de redução do desenvolvimento de pragas resistentes. |
Country Status (5)
| Country | Link |
|---|---|
| US (1) | US20100210460A1 (pt) |
| CN (1) | CN102317461A (pt) |
| BR (1) | BRPI1008674A2 (pt) |
| CA (1) | CA2751724A1 (pt) |
| WO (1) | WO2010096613A1 (pt) |
Families Citing this family (10)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US20150361447A1 (en) * | 2013-01-25 | 2015-12-17 | Pioneer Hi-Breed International, Inc. | Maize event dp-032218-9 and methods for detection thereof |
| US20150361446A1 (en) * | 2013-01-25 | 2015-12-17 | Pioneer-Hi-Bred International and E.I. Dupont De Nemours & Company | Maize event dp-033121-3 and methods for detection thereof |
| CN105264079B (zh) * | 2013-02-13 | 2019-10-18 | 阿则耐克斯公司 | Axmi184用于防治根虫昆虫的用途 |
| WO2014182473A1 (en) * | 2013-05-08 | 2014-11-13 | Monsanto Technology Llc | Compositions and methods for deploying a transgenic refuge seed blend |
| WO2016133742A1 (en) * | 2015-02-18 | 2016-08-25 | Pioneer Hi Bred International Inc | Enhancing the effectiveness of blended refuge |
| US20180255721A1 (en) * | 2015-08-24 | 2018-09-13 | Pioneer Hi-Bred International, Inc. | Crop product development and seed treatments |
| US20180303089A1 (en) * | 2015-10-13 | 2018-10-25 | Imerys Filtration Minerals, Inc. | Compositions and methods for protecting plants from organisms |
| CN107090464B (zh) * | 2016-02-18 | 2020-06-23 | 中国种子集团有限公司 | 抗虫抗除草剂玉米转化事件及其创制方法和检测方法 |
| CN105994169B (zh) * | 2016-05-31 | 2019-04-23 | 中国农业科学院植物保护研究所 | 一种利用嫩玉米穗饲养大螟的方法 |
| CN112438198A (zh) * | 2019-08-30 | 2021-03-05 | 中国农业大学 | 利用杂交不亲和基因在制备抗虫转基因玉米庇护所中的应用 |
Family Cites Families (68)
| Publication number | Priority date | Publication date | Assignee | Title |
|---|---|---|---|---|
| US3710511A (en) | 1971-04-21 | 1973-01-16 | Univ Illinois | Procedures for use of genic male sterility in production of commercial hybrid maize |
| US3861709A (en) | 1973-07-12 | 1975-01-21 | Amsted Ind Inc | Shiftable fifth wheel construction |
| US4936904A (en) | 1980-05-12 | 1990-06-26 | Carlson Glenn R | Aryl-4-oxonicotinates useful for inducing male sterility in cereal grain plants |
| US4654465A (en) | 1985-07-18 | 1987-03-31 | Agracetus | Genic male-sterile maize |
| US4727219A (en) | 1986-11-28 | 1988-02-23 | Agracetus | Genic male-sterile maize using a linked marker gene |
| NZ227835A (en) | 1988-02-03 | 1992-09-25 | Paladin Hybrids Inc | Antisense gene systems of pollination control for hybrid seed production |
| IE960441L (en) | 1988-12-27 | 1990-06-27 | Takeda Chemical Industries Ltd | Guanidine derivatives, their production and insecticides |
| ATE294872T1 (de) | 1989-02-02 | 2005-05-15 | Pioneer Hi Bred Int | Molekulare verfahren zur vermehrung von hybriden saaten |
| US5188960A (en) | 1989-06-27 | 1993-02-23 | Mycogen Corporation | Bacillus thuringiensis isolate active against lepidopteran pests, and genes encoding novel lepidopteran-active toxins |
| PL295547A1 (pt) | 1990-04-26 | 1992-10-05 | Plant Genetic Systems Nv | |
| US5432068A (en) | 1990-06-12 | 1995-07-11 | Pioneer Hi-Bred International, Inc. | Control of male fertility using externally inducible promoter sequences |
| TW198724B (pt) | 1990-10-24 | 1993-01-21 | Ciba Geigy Ag | |
| US5277905A (en) | 1991-01-16 | 1994-01-11 | Mycogen Corporation | Coleopteran-active bacillus thuringiensis isolate |
| DE69227911T2 (de) | 1991-08-02 | 1999-05-12 | Kubota Corp., Tokio/Tokyo | Neuer mikroorganismus und insektizid |
| UA48104C2 (uk) | 1991-10-04 | 2002-08-15 | Новартіс Аг | Фрагмент днк, який містить послідовність,що кодує інсектицидний протеїн, оптимізовану для кукурудзи,фрагмент днк, який забезпечує направлену бажану для серцевини стебла експресію зв'язаного з нею структурного гена в рослині, фрагмент днк, який забезпечує специфічну для пилку експресію зв`язаного з нею структурного гена в рослині, рекомбінантна молекула днк, спосіб одержання оптимізованої для кукурудзи кодуючої послідовності інсектицидного протеїну, спосіб захисту рослин кукурудзи щонайменше від однієї комахи-шкідника |
| CA2124673C (en) | 1991-12-04 | 2008-08-05 | John Browse | Fatty acid desaturase genes from plants |
| TW240163B (en) | 1992-07-22 | 1995-02-11 | Syngenta Participations Ag | Oxadiazine derivatives |
| US5743477A (en) | 1992-08-27 | 1998-04-28 | Dowelanco | Insecticidal proteins and method for plant protection |
| US6372965B1 (en) | 1992-11-17 | 2002-04-16 | E.I. Du Pont De Nemours And Company | Genes for microsomal delta-12 fatty acid desaturases and hydroxylases from plants |
| AU5676394A (en) | 1992-11-20 | 1994-06-22 | Agracetus, Inc. | Transgenic cotton plants producing heterologous bioplastic |
| CA2161881C (en) | 1993-01-13 | 2001-03-27 | A. Gururaj Rao | High lysine derivatives of alpha-hordothionin |
| US5583210A (en) | 1993-03-18 | 1996-12-10 | Pioneer Hi-Bred International, Inc. | Methods and compositions for controlling plant development |
| US5849870A (en) | 1993-03-25 | 1998-12-15 | Novartis Finance Corporation | Pesticidal proteins and strains |
| US5877012A (en) | 1993-03-25 | 1999-03-02 | Novartis Finance Corporation | Class of proteins for the control of plant pests |
| US5689052A (en) | 1993-12-22 | 1997-11-18 | Monsanto Company | Synthetic DNA sequences having enhanced expression in monocotyledonous plants and method for preparation thereof |
| US5593881A (en) | 1994-05-06 | 1997-01-14 | Mycogen Corporation | Bacillus thuringiensis delta-endotoxin |
| US5792931A (en) | 1994-08-12 | 1998-08-11 | Pioneer Hi-Bred International, Inc. | Fumonisin detoxification compositions and methods |
| US6114608A (en) | 1997-03-14 | 2000-09-05 | Novartis Ag | Nucleic acid construct comprising bacillus thuringiensis cry1Ab gene |
| FR2729825A1 (fr) | 1995-01-30 | 1996-08-02 | Rhone Poulenc Agrochimie | Associations insecticides comprenant un insecticide de la famille des chloronicotinyls et un insecticide a groupe pyrazole,pyrrole ou phenylimidazole pour traiter les semences ou le sol |
| US5696144A (en) | 1995-05-01 | 1997-12-09 | Rhone-Poulenc Inc. | Protection of corn |
| EP0828835A1 (en) | 1995-06-02 | 1998-03-18 | Pioneer Hi-Bred International, Inc. | HIGH THREONINE DERIVATIVES OF $g(a)-HORDOTHIONIN |
| PL323635A1 (en) | 1995-06-02 | 1998-04-14 | Pioneer Hi Bred Int | Derivatives of alpha-hordothionine of high methionine content |
| US5981722A (en) | 1995-11-20 | 1999-11-09 | Board Of Regents For The University Of Oklahoma | Trypsin inhibitors with insecticidal properties obtained from PENTACLETHRA MACROLOBA |
| US5703049A (en) | 1996-02-29 | 1997-12-30 | Pioneer Hi-Bred Int'l, Inc. | High methionine derivatives of α-hordothionin for pathogen-control |
| US5849320A (en) | 1996-06-13 | 1998-12-15 | Novartis Corporation | Insecticidal seed coating |
| US20020177526A1 (en) * | 1996-06-13 | 2002-11-28 | Yuguang Chen | Insecticidal seed coating |
| US5876739A (en) | 1996-06-13 | 1999-03-02 | Novartis Ag | Insecticidal seed coating |
| US6570005B1 (en) | 1996-07-01 | 2003-05-27 | Mycogen Corporation | Toxins active against pests |
| JP3441899B2 (ja) | 1996-11-01 | 2003-09-02 | 理化学研究所 | 完全長cDNAライブラリーの作成方法 |
| US6232529B1 (en) | 1996-11-20 | 2001-05-15 | Pioneer Hi-Bred International, Inc. | Methods of producing high-oil seed by modification of starch levels |
| IL132039A0 (en) | 1997-04-03 | 2001-03-19 | Novartis Ag | Plant pest control |
| US6057491A (en) | 1997-05-29 | 2000-05-02 | Borad Of Regents For University Of Oklahoma | Protein having insecticidal activities and method of use |
| US6218188B1 (en) | 1997-11-12 | 2001-04-17 | Mycogen Corporation | Plant-optimized genes encoding pesticidal toxins |
| ES2256972T3 (es) | 1997-11-18 | 2006-07-16 | Pioneer Hi-Bred International, Inc. | Metodo novedoso para la integracion de un adn foraneo dentro de genomas de eucariotas. |
| CA2309719A1 (en) | 1997-11-18 | 1999-05-27 | Pioneer Hi-Bred International, Inc. | Targeted manipulation of genes in plants |
| AU745238C (en) | 1997-11-18 | 2003-02-27 | Pioneer Hi-Bred International, Inc. | Mobilization of viral genomes from T-DNA using site-specific recombination systems |
| WO1999025821A1 (en) | 1997-11-18 | 1999-05-27 | Pioneer Hi-Bred International, Inc. | Compositions and methods for genetic modification of plants |
| US6023013A (en) | 1997-12-18 | 2000-02-08 | Monsanto Company | Insect-resistant transgenic plants |
| US6437223B1 (en) | 1998-03-13 | 2002-08-20 | Syngenta Participations Ag | Inbred maize line 2070BT |
| WO1999061619A2 (en) | 1998-05-22 | 1999-12-02 | Pioneer Hi-Bred International, Inc. | Cell cycle genes, proteins and uses thereof |
| US6518487B1 (en) | 1998-09-23 | 2003-02-11 | Pioneer Hi-Bred International, Inc. | Cyclin D polynucleotides, polypeptides and uses thereof |
| US6657046B1 (en) | 2000-01-06 | 2003-12-02 | Monsanto Technology Llc | Insect inhibitory lipid acyl hydrolases |
| MXPA02006752A (es) | 2000-01-06 | 2004-09-10 | Monsanto Technology Llc | Preparacion de proteinas y permutenias desalergenizadas. |
| US6551962B1 (en) * | 2000-10-06 | 2003-04-22 | Monsanto Technology Llc | Method for deploying a transgenic refuge |
| US7605304B2 (en) | 2000-10-24 | 2009-10-20 | E.I. Du Pont De Nemours And Company | Genes encoding novel bacillus thuringiensis proteins with pesticidal activity against coleopterans |
| CN102212534A (zh) | 2000-10-30 | 2011-10-12 | 弗迪亚股份有限公司 | 新的草甘膦n-乙酰转移酶(gat)基因 |
| US7462481B2 (en) | 2000-10-30 | 2008-12-09 | Verdia, Inc. | Glyphosate N-acetyltransferase (GAT) genes |
| US6858778B1 (en) | 2000-11-07 | 2005-02-22 | Pioneer Hi-Bred International, Inc. | Plants transformed with a DNA construct comprising a nucleic acid molecule encoding an 18 kD α-globulin |
| US7009087B1 (en) | 2000-12-01 | 2006-03-07 | Pioneer Hi-Bred International, Inc. | Compositions and methods for altering the disulfide status of proteins |
| US6865556B2 (en) * | 2001-02-09 | 2005-03-08 | Monsanto Technology Llc | Identification of seeds or plants using phenotypic markers |
| AR035799A1 (es) | 2001-03-30 | 2004-07-14 | Syngenta Participations Ag | Toxinas insecticidas aisladas de bacillus thuringiensis y sus usos. |
| US7230167B2 (en) | 2001-08-31 | 2007-06-12 | Syngenta Participations Ag | Modified Cry3A toxins and nucleic acid sequences coding therefor |
| BRPI0308104A2 (pt) | 2002-03-06 | 2016-06-28 | Syngenta Participations Ag | novas toxinas vip 3 e métodos de uso |
| AR039501A1 (es) | 2002-04-30 | 2005-02-23 | Verdia Inc | Genes de glifosato n-acetil transferasa (gat) |
| CA2521235A1 (en) | 2003-04-04 | 2004-10-14 | Syngenta Limited | Improved method of resistance management for transgenic crops |
| WO2005094340A2 (en) * | 2004-03-29 | 2005-10-13 | Pioneer Hi-Bred International, Inc. | Method of reducing insect resistant pests in transgenic crops |
| EP1891213A2 (en) | 2005-06-10 | 2008-02-27 | The University Of York | Lipase polypeptide |
| CN101568642A (zh) * | 2006-12-22 | 2009-10-28 | 先锋高级育种国际公司 | 转基因作物的抗性治理策略 |
-
2010
- 2010-02-19 CN CN2010800082355A patent/CN102317461A/zh active Pending
- 2010-02-19 CA CA2751724A patent/CA2751724A1/en not_active Abandoned
- 2010-02-19 BR BRPI1008674-9A patent/BRPI1008674A2/pt not_active IP Right Cessation
- 2010-02-19 WO PCT/US2010/024661 patent/WO2010096613A1/en not_active Ceased
- 2010-02-19 US US12/708,771 patent/US20100210460A1/en not_active Abandoned
Also Published As
| Publication number | Publication date |
|---|---|
| CA2751724A1 (en) | 2010-08-26 |
| CN102317461A (zh) | 2012-01-11 |
| WO2010096613A1 (en) | 2010-08-26 |
| US20100210460A1 (en) | 2010-08-19 |
Similar Documents
| Publication | Publication Date | Title |
|---|---|---|
| BRPI1008949A2 (pt) | Cetoenóis haloalquilmetilenóxi-fenil-substituídos. | |
| BRPI1011414A2 (pt) | "método." | |
| BRPI1007310A2 (pt) | Compostos inseticidas. | |
| EP2501753A4 (en) | STRONG NANOPAPER | |
| BRPI1011965A2 (pt) | estrutura de entressola. | |
| BRPI0918430A2 (pt) | 4-fenil-1h-pirazóis inseticidas. | |
| BRPI1011134A2 (pt) | turbomáquina. | |
| UY33125A (es) | Diaza-espiro-[5,5]-undecanos | |
| BRPI1008674A2 (pt) | Métodos de redução do desenvolvimento de pragas resistentes. | |
| BR112012019626A2 (pt) | estrutura de plantio e métodos. | |
| IT1396487B1 (it) | Convogliatore. | |
| BRPI1013157A2 (pt) | meio de fixação. | |
| BRPI1014641A2 (pt) | arilpirrolidinas pesticidas | |
| DK2442870T3 (da) | Pyrazinooxazepin-derivater. | |
| BRPI1016192A2 (pt) | derivados de soxazol. | |
| FR2941674B1 (fr) | Nacelle de turboreacteur. | |
| BR112012000565A2 (pt) | ensaio de vírus. | |
| IL217838A (en) | Image collection | |
| IT1396642B1 (it) | Statore. | |
| BR112012000099A2 (pt) | compostos inseticidas. | |
| FR2946246B1 (fr) | Fauteuil roulant. | |
| BRPI1007268A2 (pt) | Combinações pesticidas. | |
| IT1401345B1 (it) | Bomboletta. | |
| FI20095315A0 (fi) | Iskulaite | |
| FI20095440L (fi) | Muodostusosa |
Legal Events
| Date | Code | Title | Description |
|---|---|---|---|
| B08F | Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette] |
Free format text: REFERENTE A 6A ANUIDADE. |
|
| B08K | Patent lapsed as no evidence of payment of the annual fee has been furnished to inpi [chapter 8.11 patent gazette] |
Free format text: EM VIRTUDE DO ARQUIVAMENTO PUBLICADO NA RPI 2363 DE 19-04-2016 E CONSIDERANDO AUSENCIA DE MANIFESTACAO DENTRO DOS PRAZOS LEGAIS, INFORMO QUE CABE SER MANTIDO O ARQUIVAMENTO DO PEDIDO DE PATENTE, CONFORME O DISPOSTO NO ARTIGO 12, DA RESOLUCAO 113/2013. |