BRPI1013410A2 - processos de pcr para a caracterização da região não traduzida a 5' dos genes fmr1 e fmr2 - Google Patents

processos de pcr para a caracterização da região não traduzida a 5' dos genes fmr1 e fmr2

Info

Publication number
BRPI1013410A2
BRPI1013410A2 BRPI1013410A BRPI1013410A BRPI1013410A2 BR PI1013410 A2 BRPI1013410 A2 BR PI1013410A2 BR PI1013410 A BRPI1013410 A BR PI1013410A BR PI1013410 A BRPI1013410 A BR PI1013410A BR PI1013410 A2 BRPI1013410 A2 BR PI1013410A2
Authority
BR
Brazil
Prior art keywords
fmr1
untranslated region
characterization
pcr processes
region
Prior art date
Application number
BRPI1013410A
Other languages
English (en)
Inventor
J Latham Gary
Chen Liangjing
Sah Sachin
Original Assignee
Asuragen Inc
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Asuragen Inc filed Critical Asuragen Inc
Publication of BRPI1013410A2 publication Critical patent/BRPI1013410A2/pt
Publication of BRPI1013410B1 publication Critical patent/BRPI1013410B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6813—Hybridisation assays
    • C12Q1/6827—Hybridisation assays for detection of mutation or polymorphism
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6851—Quantitative amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/6858—Allele-specific amplification
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Zoology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Wood Science & Technology (AREA)
  • Analytical Chemistry (AREA)
  • Microbiology (AREA)
  • Physics & Mathematics (AREA)
  • Molecular Biology (AREA)
  • Immunology (AREA)
  • Biotechnology (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Genetics & Genomics (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

Abstract

processos de pcr para a caracterização da região não traduzida a 5' dos genes fmr1 e fmr2 a presente invenção refere-se a processos de determinação da presença e da posição de agg ou elementos de interrupção dentro de uma região de repetição com três nucleotídeos (por exemplo, cgg) e a processos de determinação do número de repetições presentes nesta região, através da amplificação de um conjunto de produtos com um conjunto de iniciadores do qual pelo menos um compreende uma parte da região de repetição de cgg e da resolução dos produtos para produzir uma representação de tamanho e abundância do produto .
BRPI1013410-7A 2009-03-24 2010-02-16 Método de analisar se uma sequência de interrupção está presente em pelo menos uma região rica em cgg compreendida por pelo menos um molde em uma amostra BRPI1013410B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
US16297709P 2009-03-24 2009-03-24
US61/162,977 2009-03-24
PCT/US2010/000426 WO2010110835A1 (en) 2009-03-24 2010-02-16 Pcr methods for characterizing the 5' untranslated region of the fmr1 and fmr2 genes

Publications (2)

Publication Number Publication Date
BRPI1013410A2 true BRPI1013410A2 (pt) 2019-07-02
BRPI1013410B1 BRPI1013410B1 (pt) 2020-10-06

Family

ID=42313963

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1013410-7A BRPI1013410B1 (pt) 2009-03-24 2010-02-16 Método de analisar se uma sequência de interrupção está presente em pelo menos uma região rica em cgg compreendida por pelo menos um molde em uma amostra

Country Status (11)

Country Link
US (2) US8679757B2 (pt)
EP (1) EP2411532B1 (pt)
KR (1) KR20110138387A (pt)
CN (2) CN102449171B (pt)
AU (1) AU2010229334B2 (pt)
BR (1) BRPI1013410B1 (pt)
ES (1) ES2629400T3 (pt)
IL (1) IL215296A (pt)
RU (1) RU2011142773A (pt)
WO (1) WO2010110835A1 (pt)
ZA (1) ZA201107298B (pt)

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JP5588970B2 (ja) 2008-05-27 2014-09-10 ダコ・デンマーク・エー／エス ハイブリダイゼーション組成物及び方法
US9303287B2 (en) 2009-02-26 2016-04-05 Dako Denmark A/S Compositions and methods for RNA hybridization applications
EP2488665B1 (en) 2009-10-16 2017-12-13 National University of Singapore Screening method for trinucleotide repeat sequences
EP3460074B1 (en) * 2010-02-05 2020-10-07 Quest Diagnostics Investments Incorporated Method to detect repeat sequence motifs in nucleic acid
WO2012047970A1 (en) * 2010-10-05 2012-04-12 Board Of Trustees Of Michigan State University Regulatory factors controlling oil biosynthesis in microalgae and their use
BR112013010585B1 (pt) 2010-10-29 2020-12-01 Asuragen, Inc. método de caracterização de um locus de fmr1 ou um locus de frm2 em uma amostra de dna e método de análise de um locus fmr1 ou um locus fmr2 de uma amostra de dna humana
US10662465B2 (en) 2011-09-30 2020-05-26 Agilent Technologies, Inc. Hybridization compositions and methods using formamide
US11118226B2 (en) * 2011-10-21 2021-09-14 Agilent Technologies, Inc. Hybridization compositions and methods
ES2759023T3 (es) * 2012-07-20 2020-05-07 Asuragen Inc Genotipificación completa de FMR1
US20160068901A1 (en) * 2013-05-01 2016-03-10 Advanced Liquid Logic, Inc. Analysis of DNA
EP3068402A4 (en) 2013-11-13 2017-11-22 Counsyl, Inc. Automated nucleic acid repeat count calling methods
AU2016297631B2 (en) * 2015-07-23 2022-08-04 Asuragen, Inc. Methods, compositions, kits, and uses for analysis of nucleic acids comprising repeating A/T-rich segments
CN108431236B (zh) 2015-11-03 2022-11-15 奥斯瑞根公司 用于重复序列的核酸大小检测的方法
RU2620944C1 (ru) * 2016-12-27 2017-05-30 Федеральное государственное бюджетное научное учреждение "Научный центр неврологии" (ФГБНУ НЦН) Способ выявления экспансии тринуклеотидных CGG-повторов в 5'-нетранслируемой, промоторной области гена FMR1 при заболевании синдрома атаксии/тремора, ассоциированного с ломкой Х-хромосомы (FXTAS)
CN108866176B (zh) * 2017-07-11 2021-09-14 北京阅微基因技术股份有限公司 一种对fmr1基因5’非翻译区cgg单元重复数量的检测体系及检测试剂盒
CN107523621A (zh) * 2017-09-04 2017-12-29 广州市达瑞生物技术股份有限公司 一种用于检测脆性x综合征的引物组及其试剂盒
GB201803019D0 (en) * 2018-02-26 2018-04-11 Univ Newcastle Methods for detecting target polynucleotides
CA3130854A1 (en) * 2019-02-26 2020-09-03 Nogra Pharma Limited Fragile x mental retardation protein interfering oligonucleotides and methods of using same
CN110157782A (zh) * 2019-03-08 2019-08-23 北京华瑞康源生物科技发展有限公司 一种用于快速检测fmr1基因cgg重复序列的引物群及pcr试剂盒
CN110577987B (zh) * 2019-06-24 2023-08-25 厦门百欧迅生物科技有限公司 一种fmr1基因cgg重复序列的检测方法及其应用
CN113215228A (zh) * 2021-02-07 2021-08-06 迈基诺（重庆）基因科技有限责任公司 脆性x综合征fmr1基因检测引物、试剂盒及其应用
CN116606920A (zh) * 2022-02-09 2023-08-18 北京大学第一医院 一种定性分析和定量分析基因rilpl1的试剂盒
EP4476360A1 (en) * 2022-02-09 2024-12-18 Laboratory Corporation of America Holdings Methods and systems for detecting methylation of fragile x
CN117683791B (zh) * 2023-12-19 2024-08-02 西湖大学 Opr33基因、蛋白及其在甘油三酯合成中的用途

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Also Published As

Publication number Publication date
ES2629400T3 (es) 2017-08-09
RU2011142773A (ru) 2013-04-27
CN105296474B (zh) 2020-05-12
AU2010229334B2 (en) 2015-08-20
EP2411532B1 (en) 2017-04-05
BRPI1013410B1 (pt) 2020-10-06
US20140248625A1 (en) 2014-09-04
US8679757B2 (en) 2014-03-25
US9783848B2 (en) 2017-10-10
AU2010229334A1 (en) 2011-10-20
US20100243451A1 (en) 2010-09-30
IL215296A0 (en) 2011-12-29
WO2010110835A1 (en) 2010-09-30
KR20110138387A (ko) 2011-12-27
EP2411532A1 (en) 2012-02-01
CN102449171B (zh) 2015-12-02
ZA201107298B (en) 2018-11-28
CN102449171A (zh) 2012-05-09
IL215296A (en) 2016-08-31
HK1220988A1 (zh) 2017-05-19
CN105296474A (zh) 2016-02-03

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