BRPI0517131A2 - mÉtodos para simplificar Ácidos nuclÉicos microbiais por modificaÇço quÍmica de citosinas - Google Patents

mÉtodos para simplificar Ácidos nuclÉicos microbiais por modificaÇço quÍmica de citosinas

Info

Publication number
BRPI0517131A2
BRPI0517131A2 BRPI0517131-8A BRPI0517131A BRPI0517131A2 BR PI0517131 A2 BRPI0517131 A2 BR PI0517131A2 BR PI0517131 A BRPI0517131 A BR PI0517131A BR PI0517131 A2 BRPI0517131 A2 BR PI0517131A2
Authority
BR
Brazil
Prior art keywords
microbial nucleic
chemical
methods
nucleic acids
nucleic acid
Prior art date
Application number
BRPI0517131-8A
Other languages
English (en)
Inventor
Douglas Spencer Millar
George Gabor L Miklos
Original Assignee
Human Genetic Signatures Pty
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Priority claimed from AU2004906915A external-priority patent/AU2004906915A0/en
Application filed by Human Genetic Signatures Pty filed Critical Human Genetic Signatures Pty
Publication of BRPI0517131A2 publication Critical patent/BRPI0517131A2/pt
Publication of BRPI0517131B1 publication Critical patent/BRPI0517131B1/pt

Links

Classifications

    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6806—Preparing nucleic acids for analysis, e.g. for polymerase chain reaction [PCR] assay
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6844—Nucleic acid amplification reactions
    • C12Q1/686—Polymerase chain reaction [PCR]
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
    • C12Q1/68—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving nucleic acids
    • C12Q1/6876—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes
    • C12Q1/6888—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms
    • C12Q1/689—Nucleic acid products used in the analysis of nucleic acids, e.g. primers or probes for detection or identification of organisms for bacteria
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2523/00—Reactions characterised by treatment of reaction samples
    • C12Q2523/10—Characterised by chemical treatment
    • C12Q2523/125—Bisulfite(s)
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2525/00—Reactions involving modified oligonucleotides, nucleic acids, or nucleotides
    • C12Q2525/10—Modifications characterised by
    • C12Q2525/117—Modifications characterised by incorporating modified base
    • C—CHEMISTRY; METALLURGY
    • C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
    • C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
    • C12Q2600/00—Oligonucleotides characterized by their use
    • C12Q2600/16—Primer sets for multiplex assays

Landscapes

  • Chemical & Material Sciences (AREA)
  • Life Sciences & Earth Sciences (AREA)
  • Organic Chemistry (AREA)
  • Proteomics, Peptides & Aminoacids (AREA)
  • Analytical Chemistry (AREA)
  • Zoology (AREA)
  • Wood Science & Technology (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Biophysics (AREA)
  • Biochemistry (AREA)
  • Microbiology (AREA)
  • Molecular Biology (AREA)
  • Biotechnology (AREA)
  • Physics & Mathematics (AREA)
  • Genetics & Genomics (AREA)
  • Immunology (AREA)
  • Bioinformatics & Cheminformatics (AREA)
  • General Engineering & Computer Science (AREA)
  • General Health & Medical Sciences (AREA)
  • Chemical Kinetics & Catalysis (AREA)
  • Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)
  • Preparation Of Compounds By Using Micro-Organisms (AREA)

Abstract

Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas: Refere-se o presente invento a um método para a simplificação de um genoma microbial ou ácido nuclélco microbial, compreendendo tratar o genoma ou ácido nucléico microbial com um agente que modifica a citosina para formar um derivado de ácido nucléico microbial e amplificar o derivado de ácido nucléico microbial para produzir uma forma simplificada de genoma ou ácido nucléico microbial.
BRPI0517131-8A 2004-12-03 2005-12-05 Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas BRPI0517131B1 (pt)

Applications Claiming Priority (3)

Application Number Priority Date Filing Date Title
AU2004906915A AU2004906915A0 (en) 2004-12-03 Detection of Microorganisms
AU2004906915 2004-12-03
PCT/AU2005/001840 WO2006058393A1 (en) 2004-12-03 2005-12-05 Methods for simplifying microbial nucleic acids by chemical modification of cytosines

Publications (2)

Publication Number Publication Date
BRPI0517131A2 true BRPI0517131A2 (pt) 2008-12-30
BRPI0517131B1 BRPI0517131B1 (pt) 2021-08-31

Family

ID=36564695

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI0517131-8A BRPI0517131B1 (pt) 2004-12-03 2005-12-05 Métodos para simplificar ácidos nucléicos microbiais por modificação química de citosinas

Country Status (14)

Country Link
US (2) US7833942B2 (pt)
EP (1) EP1828411B1 (pt)
JP (1) JP5116481B2 (pt)
KR (1) KR101293713B1 (pt)
CN (1) CN101111606B (pt)
BR (1) BRPI0517131B1 (pt)
CA (1) CA2589668C (pt)
DK (1) DK1828411T3 (pt)
ES (1) ES2399054T3 (pt)
IL (1) IL183622A0 (pt)
MX (1) MX2007006610A (pt)
NZ (1) NZ555620A (pt)
WO (1) WO2006058393A1 (pt)
ZA (1) ZA200706142B (pt)

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Also Published As

Publication number Publication date
JP5116481B2 (ja) 2013-01-09
EP1828411A1 (en) 2007-09-05
US8598088B2 (en) 2013-12-03
ES2399054T3 (es) 2013-03-25
US20090042732A1 (en) 2009-02-12
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WO2006058393A1 (en) 2006-06-08
CN101111606B (zh) 2012-05-16
MX2007006610A (es) 2007-12-12
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US20110136098A1 (en) 2011-06-09
EP1828411A4 (en) 2009-04-15
BRPI0517131B1 (pt) 2021-08-31
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