BRPI1106685A8 - técnica de vitrificação de oócitos em suporte metálico - Google Patents

técnica de vitrificação de oócitos em suporte metálico

Info

Publication number
BRPI1106685A8
BRPI1106685A8 BRPI1106685A BRPI1106685A BRPI1106685A8 BR PI1106685 A8 BRPI1106685 A8 BR PI1106685A8 BR PI1106685 A BRPI1106685 A BR PI1106685A BR PI1106685 A BRPI1106685 A BR PI1106685A BR PI1106685 A8 BRPI1106685 A8 BR PI1106685A8
Authority
BR
Brazil
Prior art keywords
oocytes
vitrification
metal support
present
support
Prior art date
Application number
BRPI1106685A
Other languages
English (en)
Inventor
Kömmling Seixas Fabiana
Carlos Deschamps João
Marques Moura De Leon Priscila
Veiras Collares Tiago
Farias Campos Vinicius
Original Assignee
Univ Pelotas Federal
Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)
Filing date
Publication date
Application filed by Univ Pelotas Federal filed Critical Univ Pelotas Federal
Priority to BRPI1106685A priority Critical patent/BRPI1106685A8/pt
Publication of BRPI1106685A2 publication Critical patent/BRPI1106685A2/pt
Publication of BRPI1106685A8 publication Critical patent/BRPI1106685A8/pt

Links

Classifications

    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/14Mechanical aspects of preservation; Apparatus or containers therefor
    • A01N1/146Non-refrigerated containers specially adapted for transporting or storing living parts whilst preserving
    • A01N1/147Carriers for immersion in cryogenic fluid for slow freezing or vitrification
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/12Chemical aspects of preservation
    • A01N1/122Preservation or perfusion media
    • A01N1/125Freeze protecting agents, e.g. cryoprotectants or osmolarity regulators
    • AHUMAN NECESSITIES
    • A01AGRICULTURE; FORESTRY; ANIMAL HUSBANDRY; HUNTING; TRAPPING; FISHING
    • A01NPRESERVATION OF BODIES OF HUMANS OR ANIMALS OR PLANTS OR PARTS THEREOF; BIOCIDES, e.g. AS DISINFECTANTS, AS PESTICIDES OR AS HERBICIDES; PEST REPELLANTS OR ATTRACTANTS; PLANT GROWTH REGULATORS
    • A01N1/00Preservation of bodies of humans or animals, or parts thereof
    • A01N1/10Preservation of living parts
    • A01N1/12Chemical aspects of preservation
    • A01N1/122Preservation or perfusion media
    • A01N1/126Physiologically active agents, e.g. antioxidants or nutrients

Landscapes

  • Life Sciences & Earth Sciences (AREA)
  • Health & Medical Sciences (AREA)
  • Engineering & Computer Science (AREA)
  • Dentistry (AREA)
  • General Health & Medical Sciences (AREA)
  • Wood Science & Technology (AREA)
  • Zoology (AREA)
  • Environmental Sciences (AREA)
  • Micro-Organisms Or Cultivation Processes Thereof (AREA)
  • Apparatus Associated With Microorganisms And Enzymes (AREA)

Abstract

técnica de vitrificação de oócitos em suporte metálico. a presente invenção refere-se a criopreservação de oócitos de mamíferos utilizando suporte metálico, e mais particularmente refere-se a estrutura metálica criada para vitrificação, servindo esta como suporte para manipulação, armazenamento e estocagem dos oócitos em palhetas; e a adição de copolímero bloqueador de gelo às solução crioprotetoras de vitrificação. existe no estado da técnica uma carência no método de acondionamento das microgotas vitrificadas contendo os oócitos, e além disso, a principal causa de crioinjúrias celulares é a formação de gelo intra e extra celular. em vista disto, a presente invenção abrange um suporte metálico, que facilita a manipulação das microgotas vitrificadas contendo os oócitos, poi permite o armazenamento em palhetas de 0,5 ml; e a adição de copolimero bloqueador de gelo nas soluções de vitrificação, a fim de diminuir as crioinjúrias causadas às células. a presente invenção refere-se à metodologia de criopreservação ultrarrápida, ao qual foi elaborada para ser utilizada em laboratórios de biotécnicas reprodutivas especializados.
BRPI1106685A 2011-10-26 2011-10-26 técnica de vitrificação de oócitos em suporte metálico BRPI1106685A8 (pt)

Priority Applications (1)

Application Number Priority Date Filing Date Title
BRPI1106685A BRPI1106685A8 (pt) 2011-10-26 2011-10-26 técnica de vitrificação de oócitos em suporte metálico

Applications Claiming Priority (1)

Application Number Priority Date Filing Date Title
BRPI1106685A BRPI1106685A8 (pt) 2011-10-26 2011-10-26 técnica de vitrificação de oócitos em suporte metálico

Publications (2)

Publication Number Publication Date
BRPI1106685A2 BRPI1106685A2 (pt) 2018-02-27
BRPI1106685A8 true BRPI1106685A8 (pt) 2019-01-15

Family

ID=62062308

Family Applications (1)

Application Number Title Priority Date Filing Date
BRPI1106685A BRPI1106685A8 (pt) 2011-10-26 2011-10-26 técnica de vitrificação de oócitos em suporte metálico

Country Status (1)

Country Link
BR (1) BRPI1106685A8 (pt)

Also Published As

Publication number Publication date
BRPI1106685A2 (pt) 2018-02-27

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Legal Events

Date Code Title Description
B03A Publication of a patent application or of a certificate of addition of invention [chapter 3.1 patent gazette]
B08F Application dismissed because of non-payment of annual fees [chapter 8.6 patent gazette]
B08G Application fees: restoration [chapter 8.7 patent gazette]
B06F Objections, documents and/or translations needed after an examination request according [chapter 6.6 patent gazette]
B11A Dismissal acc. art.33 of ipl - examination not requested within 36 months of filing
B03H Publication of an application: rectification [chapter 3.8 patent gazette]

Free format text: REFERENTE A RPI 2460 DE 27/02/218, QUANTO AO ITEM (72).

B04C Request for examination: application reinstated [chapter 4.3 patent gazette]
B07A Application suspended after technical examination (opinion) [chapter 7.1 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]
B09B Patent application refused [chapter 9.2 patent gazette]

Free format text: MANTIDO O INDEFERIMENTO UMA VEZ QUE NAO FOI APRESENTADO RECURSO DENTRO DO PRAZO LEGAL.