CA1046936A - Analyse de liquides biologiques - Google Patents

Analyse de liquides biologiques

Info

Publication number: CA1046936A
Authority: CA; Canada
Prior art keywords: reagent; clq; complex; solid phase; sample
Prior art date: 1975-05-21
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA227,481A

Other languages

English (en)

Other versions

CA227481S (en

Inventor

Pierre L. Masson

Joseph F. Heremans

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer Corp

Original Assignee

Technicon Instruments Corp

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1975-05-21

Filing date

1975-05-21

Publication date

1979-01-23

1975-05-21 Application filed by Technicon Instruments Corp filed Critical Technicon Instruments Corp

1975-05-21 Priority to CA227,481A priority Critical patent/CA1046936A/fr

1979-01-23 Application granted granted Critical

1979-01-23 Publication of CA1046936A publication Critical patent/CA1046936A/fr

Status Expired legal-status Critical Current

Links

238000004458 analytical method Methods 0.000 title claims description 8
239000013060 biological fluid Substances 0.000 title abstract description 5
239000003153 chemical reaction reagent Substances 0.000 claims abstract description 47
239000000427 antigen Substances 0.000 claims abstract description 25
102000036639 antigens Human genes 0.000 claims abstract description 24
108091007433 antigens Proteins 0.000 claims abstract description 24
239000000203 mixture Substances 0.000 claims abstract description 16
239000000463 material Substances 0.000 claims abstract description 8
238000000034 method Methods 0.000 claims description 42
239000007790 solid phase Substances 0.000 claims description 23
239000000758 substrate Substances 0.000 claims description 15
239000007788 liquid Substances 0.000 claims description 13
239000012530 fluid Substances 0.000 claims description 12
239000002245 particle Substances 0.000 claims description 11
229920000936 Agarose Polymers 0.000 claims description 10
108060003951 Immunoglobulin Proteins 0.000 claims description 9
102000018358 immunoglobulin Human genes 0.000 claims description 9
229940072221 immunoglobulins Drugs 0.000 claims description 9
239000011324 bead Substances 0.000 claims description 5
239000011248 coating agent Substances 0.000 claims description 5
238000000576 coating method Methods 0.000 claims description 5
239000004816 latex Substances 0.000 claims description 5
229920000126 latex Polymers 0.000 claims description 5
102000004190 Enzymes Human genes 0.000 claims description 3
108090000790 Enzymes Proteins 0.000 claims description 3
238000005206 flow analysis Methods 0.000 claims description 3
229920002994 synthetic fiber Polymers 0.000 claims description 3
239000005515 coenzyme Substances 0.000 claims 1
238000012875 competitive assay Methods 0.000 claims 1
230000002285 radioactive effect Effects 0.000 claims 1
210000002966 serum Anatomy 0.000 abstract description 29
BQJCRHHNABKAKU-KBQPJGBKSA-N morphine Chemical compound O([C@H]1[C@H](C=C[C@H]23)O)C4=C5[C@@]12CCN(C)[C@@H]3CC5=CC=C4O BQJCRHHNABKAKU-KBQPJGBKSA-N 0.000 description 20
239000000243 solution Substances 0.000 description 15
229960005181 morphine Drugs 0.000 description 11
238000001514 detection method Methods 0.000 description 6
230000000694 effects Effects 0.000 description 5
BVKZGUZCCUSVTD-UHFFFAOYSA-L Carbonate Chemical compound [O-]C([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-L 0.000 description 4
DHMQDGOQFOQNFH-UHFFFAOYSA-N Glycine Chemical compound NCC(O)=O DHMQDGOQFOQNFH-UHFFFAOYSA-N 0.000 description 4
206010043376 Tetanus Diseases 0.000 description 4
239000000872 buffer Substances 0.000 description 4
230000002255 enzymatic effect Effects 0.000 description 4
238000003018 immunoassay Methods 0.000 description 4
108090000623 proteins and genes Proteins 0.000 description 4
230000000717 retained effect Effects 0.000 description 4
238000012360 testing method Methods 0.000 description 4
SXRSQZLOMIGNAQ-UHFFFAOYSA-N Glutaraldehyde Chemical compound O=CCCCC=O SXRSQZLOMIGNAQ-UHFFFAOYSA-N 0.000 description 3
241000282414 Homo sapiens Species 0.000 description 3
241000283973 Oryctolagus cuniculus Species 0.000 description 3
241000700605 Viruses Species 0.000 description 3
210000004369 blood Anatomy 0.000 description 3
239000008280 blood Substances 0.000 description 3
238000012512 characterization method Methods 0.000 description 3
150000004695 complexes Chemical class 0.000 description 3
238000005859 coupling reaction Methods 0.000 description 3
150000002500 ions Chemical class 0.000 description 3
238000002360 preparation method Methods 0.000 description 3
102000004169 proteins and genes Human genes 0.000 description 3
238000000926 separation method Methods 0.000 description 3
210000002700 urine Anatomy 0.000 description 3
239000004471 Glycine Substances 0.000 description 2
241001465754 Metazoa Species 0.000 description 2
102000003992 Peroxidases Human genes 0.000 description 2
230000004520 agglutination Effects 0.000 description 2
230000008878 coupling Effects 0.000 description 2
238000010168 coupling process Methods 0.000 description 2
201000010099 disease Diseases 0.000 description 2
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 2
238000010438 heat treatment Methods 0.000 description 2
108040007629 peroxidase activity proteins Proteins 0.000 description 2
239000008363 phosphate buffer Substances 0.000 description 2
150000003839 salts Chemical class 0.000 description 2
239000007787 solid Substances 0.000 description 2
239000000126 substance Substances 0.000 description 2
ZCYVEMRRCGMTRW-UHFFFAOYSA-N 7553-56-2 Chemical compound [I] ZCYVEMRRCGMTRW-UHFFFAOYSA-N 0.000 description 1
HRPVXLWXLXDGHG-UHFFFAOYSA-N Acrylamide Chemical compound NC(=O)C=C HRPVXLWXLXDGHG-UHFFFAOYSA-N 0.000 description 1
241000894006 Bacteria Species 0.000 description 1
108091003079 Bovine Serum Albumin Proteins 0.000 description 1
241000283707 Capra Species 0.000 description 1
102000016938 Catalase Human genes 0.000 description 1
108010053835 Catalase Proteins 0.000 description 1
241000237074 Centris Species 0.000 description 1
240000003550 Eusideroxylon zwageri Species 0.000 description 1
244000182067 Fraxinus ornus Species 0.000 description 1
206010028980 Neoplasm Diseases 0.000 description 1
239000004677 Nylon Substances 0.000 description 1
ISWSIDIOOBJBQZ-UHFFFAOYSA-N Phenol Chemical compound OC1=CC=CC=C1 ISWSIDIOOBJBQZ-UHFFFAOYSA-N 0.000 description 1
239000004793 Polystyrene Substances 0.000 description 1
229920005654 Sephadex Polymers 0.000 description 1
239000012507 Sephadex™ Substances 0.000 description 1
229920002684 Sepharose Polymers 0.000 description 1
FAPWRFPIFSIZLT-UHFFFAOYSA-M Sodium chloride Chemical compound [Na+].[Cl-] FAPWRFPIFSIZLT-UHFFFAOYSA-M 0.000 description 1
101150007148 THI5 gene Proteins 0.000 description 1
244000247617 Teramnus labialis var. labialis Species 0.000 description 1
238000010521 absorption reaction Methods 0.000 description 1
SOIFLUNRINLCBN-UHFFFAOYSA-N ammonium thiocyanate Chemical compound [NH4+].[S-]C#N SOIFLUNRINLCBN-UHFFFAOYSA-N 0.000 description 1
239000007864 aqueous solution Substances 0.000 description 1
238000004380 ashing Methods 0.000 description 1
238000003556 assay Methods 0.000 description 1
238000009582 blood typing Methods 0.000 description 1
239000012888 bovine serum Substances 0.000 description 1
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201000011510 cancer Diseases 0.000 description 1
210000004027 cell Anatomy 0.000 description 1
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238000006243 chemical reaction Methods 0.000 description 1
230000000295 complement effect Effects 0.000 description 1
238000003745 diagnosis Methods 0.000 description 1
238000010790 dilution Methods 0.000 description 1
239000012895 dilution Substances 0.000 description 1
230000002500 effect on skin Effects 0.000 description 1
238000001493 electron microscopy Methods 0.000 description 1
210000003743 erythrocyte Anatomy 0.000 description 1
238000001914 filtration Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
238000010166 immunofluorescence Methods 0.000 description 1
230000005764 inhibitory process Effects 0.000 description 1
239000007924 injection Substances 0.000 description 1
238000002347 injection Methods 0.000 description 1
229910052740 iodine Inorganic materials 0.000 description 1
239000011630 iodine Substances 0.000 description 1
238000002955 isolation Methods 0.000 description 1
238000002372 labelling Methods 0.000 description 1
239000007791 liquid phase Substances 0.000 description 1
239000011259 mixed solution Substances 0.000 description 1
238000002156 mixing Methods 0.000 description 1
238000004848 nephelometry Methods 0.000 description 1
102000039446 nucleic acids Human genes 0.000 description 1
108020004707 nucleic acids Proteins 0.000 description 1
150000007523 nucleic acids Chemical class 0.000 description 1
229920001778 nylon Polymers 0.000 description 1
QYSGYZVSCZSLHT-UHFFFAOYSA-N octafluoropropane Chemical compound FC(F)(F)C(F)(F)C(F)(F)F QYSGYZVSCZSLHT-UHFFFAOYSA-N 0.000 description 1
230000003647 oxidation Effects 0.000 description 1
238000007254 oxidation reaction Methods 0.000 description 1
239000002504 physiological saline solution Substances 0.000 description 1
229920002401 polyacrylamide Polymers 0.000 description 1
229920000058 polyacrylate Polymers 0.000 description 1
229920002223 polystyrene Polymers 0.000 description 1
238000001556 precipitation Methods 0.000 description 1
125000002924 primary amino group Chemical group [H]N([H])* 0.000 description 1
238000000746 purification Methods 0.000 description 1
239000000376 reactant Substances 0.000 description 1
HRZFUMHJMZEROT-UHFFFAOYSA-L sodium disulfite Chemical compound [Na+].[Na+].[O-]S(=O)S([O-])(=O)=O HRZFUMHJMZEROT-UHFFFAOYSA-L 0.000 description 1
235000010262 sodium metabisulphite Nutrition 0.000 description 1
239000004296 sodium metabisulphite Substances 0.000 description 1
238000001179 sorption measurement Methods 0.000 description 1
241000894007 species Species 0.000 description 1
239000006228 supernatant Substances 0.000 description 1
238000010998 test method Methods 0.000 description 1
239000003053 toxin Substances 0.000 description 1
239000002699 waste material Substances 0.000 description 1

Landscapes

Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)

CA227,481A 1975-05-21 1975-05-21 Analyse de liquides biologiques Expired CA1046936A (fr)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA227,481A CA1046936A (fr)	1975-05-21	1975-05-21	Analyse de liquides biologiques

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
CA227,481A CA1046936A (fr)	1975-05-21	1975-05-21	Analyse de liquides biologiques

Publications (1)

Publication Number	Publication Date
CA1046936A true CA1046936A (fr)	1979-01-23

Family

ID=4103138

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA227,481A Expired CA1046936A (fr)	1975-05-21	1975-05-21	Analyse de liquides biologiques

Country Status (1)

Country	Link
CA (1)	CA1046936A (fr)

1975
- 1975-05-21 CA CA227,481A patent/CA1046936A/fr not_active Expired

Publication	Publication Date	Title
US4143124A (en)	1979-03-06	Antigen-antibody analysis with solid phase rf and c1q
US4283383A (en)	1981-08-11	Analysis of biological fluids
US4434236A (en)	1984-02-28	Immunoassay wherein labeled antibody is displaced from immobilized analyte-analogue
US4020151A (en)	1977-04-26	Method for quantitation of antigens or antibodies on a solid surface
US4138213A (en)	1979-02-06	Agglutination immunoassay of immune complex with RF or Clq
JPH087215B2 (ja)	1996-01-29	抗原および／又は抗体の検出方法および検出用の試験キット
JPH0421818B2 (fr)	1992-04-14
JP2636331B2 (ja)	1997-07-30	抗原特異的な抗体の一段階測定法およびそれに適する試薬
US4366242A (en)	1982-12-28	Method and agent for the immunological determination of enzymes
US4865997A (en)	1989-09-12	Assay for ligands by separating bound and free tracer from sample
EP0227240A1 (fr)	1987-07-01	Procédé pour un essai immunologique d'une analyte macromoléculaire
US4624916A (en)	1986-11-25	Process and composition for the rapid quantitation of small levels of creative kinase-MB isoenzyme
CA1300499C (fr)	1992-05-12	Reactif insoluble dans l'eau, elements qui en contiennent et methode d'utilisation
US4522922A (en)	1985-06-11	Soluble assay
JPS5984162A (ja)	1984-05-15	免疫分析法
US3872225A (en)	1975-03-18	Process of viral diagnosis and reagent
US4714672A (en)	1987-12-22	Immunoassays involving complement lysing of chromophore containing microcapsules
CA1046936A (fr)	1979-01-23	Analyse de liquides biologiques
JPH0421819B2 (fr)	1992-04-14
Wehler et al.	1981	The use of solid phase Clq and horseradish peroxidase-conjugated goat anti-IgG for the detection of immune complexes in human serum
Eckert	1976	Radioimmunoassay
US4409200A (en)	1983-10-11	Reverse transcriptase from human milk, method for its purification, and its use in the detection of breast cancer
NISHIMURA et al.	1982	Enzyme immunoassay for manganese-superoxide dismutase in serum and urine
JP3681873B2 (ja)	2005-08-10	物質の測定方法
KR940008091B1 (ko)	1994-09-02	리간드의 면역학적 검출방법