CA1051326A - Method and reagent for reducing errors in enzymatic assays - Google Patents

Method and reagent for reducing errors in enzymatic assays

Info

Publication number: CA1051326A
Authority: CA; Canada
Prior art keywords: glucose; assay; reagent; sample; compound
Prior art date: 1974-04-29
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA220,039A

Other languages

English (en)

French (fr)

Other versions

CA220039S (en

Inventor

Charles Allain

Carl Henson

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Abbott Laboratories

Original Assignee

Abbott Laboratories

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1974-04-29

Filing date

1975-02-13

Publication date

1979-03-27

1975-02-13 Application filed by Abbott Laboratories filed Critical Abbott Laboratories

1978-10-25 Priority to CA314,224A priority Critical patent/CA1053548A/en

1979-03-27 Application granted granted Critical

1979-03-27 Publication of CA1051326A publication Critical patent/CA1051326A/en

Status Expired legal-status Critical Current

Links

239000003153 chemical reaction reagent Substances 0.000 title claims abstract description 38
238000000034 method Methods 0.000 title claims abstract description 22
238000007824 enzymatic assay Methods 0.000 title claims description 9
229930027945 nicotinamide-adenine dinucleotide Natural products 0.000 claims abstract description 58
BAWFJGJZGIEFAR-NNYOXOHSSA-N NAD zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-N 0.000 claims abstract description 37
229950006238 nadide Drugs 0.000 claims abstract description 37
238000003556 assay Methods 0.000 claims abstract description 31
WQZGKKKJIJFFOK-GASJEMHNSA-N Glucose Natural products OC[C@H]1OC(O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-GASJEMHNSA-N 0.000 claims abstract description 28
239000008103 glucose Substances 0.000 claims abstract description 28
LCTONWCANYUPML-UHFFFAOYSA-M Pyruvate Chemical compound CC(=O)C([O-])=O LCTONWCANYUPML-UHFFFAOYSA-M 0.000 claims abstract description 24
102000004420 Creatine Kinase Human genes 0.000 claims abstract description 22
108010042126 Creatine kinase Proteins 0.000 claims abstract description 22
BOPGDPNILDQYTO-NNYOXOHSSA-N nicotinamide-adenine dinucleotide Chemical compound C1=CCC(C(=O)N)=CN1[C@H]1[C@H](O)[C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]2[C@H]([C@@H](O)[C@@H](O2)N2C3=NC=NC(N)=C3N=C2)O)O1 BOPGDPNILDQYTO-NNYOXOHSSA-N 0.000 claims abstract description 22
238000006243 chemical reaction Methods 0.000 claims abstract description 20
SOWBFZRMHSNYGE-UHFFFAOYSA-N oxamic acid Chemical class NC(=O)C(O)=O SOWBFZRMHSNYGE-UHFFFAOYSA-N 0.000 claims abstract description 19
101710088194 Dehydrogenase Proteins 0.000 claims abstract description 14
150000003839 salts Chemical class 0.000 claims abstract description 11
239000000470 constituent Substances 0.000 claims abstract description 8
230000008034 disappearance Effects 0.000 claims abstract description 4
MUBZPKHOEPUJKR-UHFFFAOYSA-N Oxalic acid Chemical compound OC(=O)C(O)=O MUBZPKHOEPUJKR-UHFFFAOYSA-N 0.000 claims description 30
150000001875 compounds Chemical class 0.000 claims description 22
ZKHQWZAMYRWXGA-UHFFFAOYSA-N Adenosine triphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(=O)OP(O)(O)=O)C(O)C1O ZKHQWZAMYRWXGA-UHFFFAOYSA-N 0.000 claims description 16
NBSCHQHZLSJFNQ-GASJEMHNSA-N D-Glucose 6-phosphate Chemical compound OC1O[C@H](COP(O)(O)=O)[C@@H](O)[C@H](O)[C@H]1O NBSCHQHZLSJFNQ-GASJEMHNSA-N 0.000 claims description 16
VFRROHXSMXFLSN-UHFFFAOYSA-N Glc6P Natural products OP(=O)(O)OCC(O)C(O)C(O)C(O)C=O VFRROHXSMXFLSN-UHFFFAOYSA-N 0.000 claims description 16
DRBBFCLWYRJSJZ-UHFFFAOYSA-N N-phosphocreatine Chemical compound OC(=O)CN(C)C(=N)NP(O)(O)=O DRBBFCLWYRJSJZ-UHFFFAOYSA-N 0.000 claims description 14
102000005548 Hexokinase Human genes 0.000 claims description 12
108700040460 Hexokinases Proteins 0.000 claims description 12
CVSVTCORWBXHQV-UHFFFAOYSA-N creatine Chemical compound NC(=[NH2+])N(C)CC([O-])=O CVSVTCORWBXHQV-UHFFFAOYSA-N 0.000 claims description 12
102100031126 6-phosphogluconolactonase Human genes 0.000 claims description 11
108010029731 6-phosphogluconolactonase Proteins 0.000 claims description 11
108010018962 Glucosephosphate Dehydrogenase Proteins 0.000 claims description 11
XTWYTFMLZFPYCI-KQYNXXCUSA-N 5'-adenylphosphoric acid Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O XTWYTFMLZFPYCI-KQYNXXCUSA-N 0.000 claims description 9
XTWYTFMLZFPYCI-UHFFFAOYSA-N Adenosine diphosphate Natural products C1=NC=2C(N)=NC=NC=2N1C1OC(COP(O)(=O)OP(O)(O)=O)C(O)C1O XTWYTFMLZFPYCI-UHFFFAOYSA-N 0.000 claims description 9
230000000694 effects Effects 0.000 claims description 9
ZKHQWZAMYRWXGA-KQYNXXCUSA-N Adenosine triphosphate Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP(O)(=O)OP(O)(=O)OP(O)(O)=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-N 0.000 claims description 8
229960001456 adenosine triphosphate Drugs 0.000 claims description 8
235000006408 oxalic acid Nutrition 0.000 claims description 7
229960003624 creatine Drugs 0.000 claims description 6
239000006046 creatine Substances 0.000 claims description 6
230000002452 interceptive effect Effects 0.000 claims description 6
XLYOFNOQVPJJNP-UHFFFAOYSA-N water Substances O XLYOFNOQVPJJNP-UHFFFAOYSA-N 0.000 claims description 4
239000000203 mixture Substances 0.000 claims description 3
230000003647 oxidation Effects 0.000 claims description 3
238000007254 oxidation reaction Methods 0.000 claims description 3
230000026731 phosphorylation Effects 0.000 claims description 3
238000006366 phosphorylation reaction Methods 0.000 claims description 3
159000000003 magnesium salts Chemical class 0.000 claims description 2
230000001590 oxidative effect Effects 0.000 claims description 2
230000000865 phosphorylative effect Effects 0.000 claims description 2
239000007787 solid Substances 0.000 claims description 2
QGZKDVFQNNGYKY-UHFFFAOYSA-N Ammonia Chemical compound N QGZKDVFQNNGYKY-UHFFFAOYSA-N 0.000 abstract description 10
JVTAAEKCZFNVCJ-UHFFFAOYSA-M Lactate Chemical compound CC(O)C([O-])=O JVTAAEKCZFNVCJ-UHFFFAOYSA-M 0.000 abstract description 6
229910021529 ammonia Inorganic materials 0.000 abstract description 5
108010021582 Glucokinase Proteins 0.000 abstract description 4
WQZGKKKJIJFFOK-VFUOTHLCSA-N beta-D-glucose Chemical compound OC[C@H]1O[C@@H](O)[C@H](O)[C@@H](O)[C@@H]1O WQZGKKKJIJFFOK-VFUOTHLCSA-N 0.000 abstract description 2
238000005259 measurement Methods 0.000 abstract description 2
229940101270 nicotinamide adenine dinucleotide (nad) Drugs 0.000 abstract description 2
239000000523 sample Substances 0.000 description 19
XJLXINKUBYWONI-NNYOXOHSSA-N NADP zwitterion Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-NNYOXOHSSA-N 0.000 description 9
229940045189 glucose-6-phosphate Drugs 0.000 description 9
ZKHQWZAMYRWXGA-KQYNXXCUSA-J ATP(4-) Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](COP([O-])(=O)OP([O-])(=O)OP([O-])([O-])=O)[C@@H](O)[C@H]1O ZKHQWZAMYRWXGA-KQYNXXCUSA-J 0.000 description 8
BAWFJGJZGIEFAR-NNYOXOHSSA-O NAD(+) Chemical compound NC(=O)C1=CC=C[N+]([C@H]2[C@@H]([C@H](O)[C@@H](COP(O)(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 BAWFJGJZGIEFAR-NNYOXOHSSA-O 0.000 description 4
239000002253 acid Substances 0.000 description 4
OIRDTQYFTABQOQ-KQYNXXCUSA-N adenosine Chemical compound C1=NC=2C(N)=NC=NC=2N1[C@@H]1O[C@H](CO)[C@@H](O)[C@H]1O OIRDTQYFTABQOQ-KQYNXXCUSA-N 0.000 description 4
229910019142 PO4 Inorganic materials 0.000 description 3
XJLXINKUBYWONI-DQQFMEOOSA-N [[(2r,3r,4r,5r)-5-(6-aminopurin-9-yl)-3-hydroxy-4-phosphonooxyoxolan-2-yl]methoxy-hydroxyphosphoryl] [(2s,3r,4s,5s)-5-(3-carbamoylpyridin-1-ium-1-yl)-3,4-dihydroxyoxolan-2-yl]methyl phosphate Chemical compound NC(=O)C1=CC=C[N+]([C@@H]2[C@H]([C@@H](O)[C@H](COP([O-])(=O)OP(O)(=O)OC[C@@H]3[C@H]([C@@H](OP(O)(O)=O)[C@@H](O3)N3C4=NC=NC(N)=C4N=C3)O)O2)O)=C1 XJLXINKUBYWONI-DQQFMEOOSA-N 0.000 description 3
238000002835 absorbance Methods 0.000 description 3
239000004615 ingredient Substances 0.000 description 3
NBIIXXVUZAFLBC-UHFFFAOYSA-K phosphate Chemical compound [O-]P([O-])([O-])=O NBIIXXVUZAFLBC-UHFFFAOYSA-K 0.000 description 3
239000010452 phosphate Substances 0.000 description 3
239000002126 C01EB10 - Adenosine Substances 0.000 description 2
FYYHWMGAXLPEAU-UHFFFAOYSA-N Magnesium Chemical compound [Mg] FYYHWMGAXLPEAU-UHFFFAOYSA-N 0.000 description 2
239000012190 activator Substances 0.000 description 2
229960005305 adenosine Drugs 0.000 description 2
VFRROHXSMXFLSN-KCDKBNATSA-N aldehydo-D-galactose 6-phosphate Chemical compound OP(=O)(O)OC[C@@H](O)[C@H](O)[C@H](O)[C@@H](O)C=O VFRROHXSMXFLSN-KCDKBNATSA-N 0.000 description 2
239000000872 buffer Substances 0.000 description 2
IRXRGVFLQOSHOH-UHFFFAOYSA-L dipotassium;oxalate Chemical compound [K+].[K+].[O-]C(=O)C([O-])=O IRXRGVFLQOSHOH-UHFFFAOYSA-L 0.000 description 2
230000002255 enzymatic effect Effects 0.000 description 2
239000011777 magnesium Substances 0.000 description 2
229910052749 magnesium Inorganic materials 0.000 description 2
238000007086 side reaction Methods 0.000 description 2
239000000243 solution Substances 0.000 description 2
JVTAAEKCZFNVCJ-REOHCLBHSA-M (S)-lactate Chemical compound C[C@H](O)C([O-])=O JVTAAEKCZFNVCJ-REOHCLBHSA-M 0.000 description 1
BIRSGZKFKXLSJQ-SQOUGZDYSA-N 6-Phospho-D-gluconate Chemical compound OP(=O)(O)OC[C@@H](O)[C@@H](O)[C@H](O)[C@@H](O)C(O)=O BIRSGZKFKXLSJQ-SQOUGZDYSA-N 0.000 description 1
229930024421 Adenine Natural products 0.000 description 1
GFFGJBXGBJISGV-UHFFFAOYSA-N Adenine Chemical compound NC1=NC=NC2=C1N=CN2 GFFGJBXGBJISGV-UHFFFAOYSA-N 0.000 description 1
108010020056 Hydrogenase Proteins 0.000 description 1
108090000854 Oxidoreductases Proteins 0.000 description 1
102000004316 Oxidoreductases Human genes 0.000 description 1
150000007513 acids Chemical class 0.000 description 1
229960000643 adenine Drugs 0.000 description 1
230000008033 biological extinction Effects 0.000 description 1
239000012472 biological sample Substances 0.000 description 1
230000015572 biosynthetic process Effects 0.000 description 1
239000007853 buffer solution Substances 0.000 description 1
239000005515 coenzyme Substances 0.000 description 1
230000003247 decreasing effect Effects 0.000 description 1
239000012153 distilled water Substances 0.000 description 1
VHJLVAABSRFDPM-ZXZARUISSA-N dithioerythritol Chemical compound SC[C@H](O)[C@H](O)CS VHJLVAABSRFDPM-ZXZARUISSA-N 0.000 description 1
229940079593 drug Drugs 0.000 description 1
239000003814 drug Substances 0.000 description 1
238000009472 formulation Methods 0.000 description 1
230000000977 initiatory effect Effects 0.000 description 1
229940116871 l-lactate Drugs 0.000 description 1
230000001404 mediated effect Effects 0.000 description 1
239000002773 nucleotide Substances 0.000 description 1
125000003729 nucleotide group Chemical group 0.000 description 1
230000003287 optical effect Effects 0.000 description 1
XAEFZNCEHLXOMS-UHFFFAOYSA-M potassium benzoate Chemical compound [K+].[O-]C(=O)C1=CC=CC=C1 XAEFZNCEHLXOMS-UHFFFAOYSA-M 0.000 description 1
239000000843 powder Substances 0.000 description 1
230000003405 preventing effect Effects 0.000 description 1
230000002441 reversible effect Effects 0.000 description 1
210000002966 serum Anatomy 0.000 description 1
239000002904 solvent Substances 0.000 description 1
210000003371 toe Anatomy 0.000 description 1
239000001226 triphosphate Substances 0.000 description 1
235000011178 triphosphate Nutrition 0.000 description 1
UNXRWKVEANCORM-UHFFFAOYSA-N triphosphoric acid Chemical compound OP(O)(=O)OP(O)(=O)OP(O)(O)=O UNXRWKVEANCORM-UHFFFAOYSA-N 0.000 description 1

Classifications

- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/48—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase
- C12Q1/50—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving transferase involving creatine phosphokinase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/26—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase
- C12Q1/32—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving oxidoreductase involving dehydrogenase
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12Q—MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEIC ACIDS OR MICROORGANISMS; COMPOSITIONS OR TEST PAPERS THEREFOR; PROCESSES OF PREPARING SUCH COMPOSITIONS; CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL OR ENZYMOLOGICAL PROCESSES
- C12Q1/00—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions
- C12Q1/54—Measuring or testing processes involving enzymes, nucleic acids or microorganisms; Compositions therefor; Processes of preparing such compositions involving glucose or galactose

Landscapes

Chemical & Material Sciences (AREA)
Organic Chemistry (AREA)
Life Sciences & Earth Sciences (AREA)
Health & Medical Sciences (AREA)
Zoology (AREA)
Wood Science & Technology (AREA)
Proteomics, Peptides & Aminoacids (AREA)
Engineering & Computer Science (AREA)
Microbiology (AREA)
Biochemistry (AREA)
Physics & Mathematics (AREA)
Molecular Biology (AREA)
Biotechnology (AREA)
Biophysics (AREA)
Analytical Chemistry (AREA)
Immunology (AREA)
Bioinformatics & Cheminformatics (AREA)
General Engineering & Computer Science (AREA)
General Health & Medical Sciences (AREA)
Genetics & Genomics (AREA)
Emergency Medicine (AREA)
Measuring Or Testing Involving Enzymes Or Micro-Organisms (AREA)

CA220,039A 1974-04-29 1975-02-13 Method and reagent for reducing errors in enzymatic assays Expired CA1051326A (en)

Priority Applications (1)

Application Number	Priority Date	Filing Date	Title
CA314,224A CA1053548A (en)	1974-04-29	1978-10-25	Method and reagent for reducing errors in enzymatic assays

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US05/465,039 US3956069A (en)	1974-04-29	1974-04-29	Enzymatic assays for glucose, creatine phosphokinase or plasma ammonia

Publications (1)

Publication Number	Publication Date
CA1051326A true CA1051326A (en)	1979-03-27

Family

ID=23846267

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA220,039A Expired CA1051326A (en)	1974-04-29	1975-02-13	Method and reagent for reducing errors in enzymatic assays

Country Status (7)

Country	Link
US (1)	US3956069A (2)
JP (1)	JPS5110996A (2)
CA (1)	CA1051326A (2)
DE (1)	DE2518862B2 (2)
FR (1)	FR2269080B1 (2)
GB (1)	GB1491313A (2)
SE (2)	SE405046B (2)

Families Citing this family (22)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
DE2700952C2 (de) *	1977-01-12	1979-03-15	Gesellschaft Fuer Kernenergieverwertung In Schiffbau Und Schiffahrt Mbh, 2054 Geesthacht-Tesperhude	Verfahren zur Identifikation undichter Komponenten aus einem Vielkomponentensystem
DE2718588C3 (de) *	1977-04-26	1979-11-08	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und Reagens zur Bestimmung von Harnsäure
US4189536A (en) *	1977-11-09	1980-02-19	Hycel, Inc.	Reagent systems, enzymatic assays and compositions therefor
US4242446A (en) *	1978-07-26	1980-12-30	Coulter Electronics, Inc.	Method for determining a substance in a biological fluid and reagent combination for use in the method
DE2834705B1 (de) *	1978-08-08	1980-01-24	Boehringer Mannheim Gmbh	Verfahren und Reagenz zur Durchfuehrung enzymatischer Bestimmungen
DE2834704A1 (de) *	1978-08-08	1980-02-21	Boehringer Mannheim Gmbh	Verfahren zur quantitativen enzymatischen bestimmung von adp
US4241179A (en) *	1978-08-14	1980-12-23	Coulter Electronics, Inc.	Method for determining a transaminase in a biological fluid and reagent combination for use in the method
US4322496A (en) *	1980-04-17	1982-03-30	Eastman Kodak Company	Inhibition of lactate oxidase
DE3025170A1 (de) *	1980-07-03	1982-01-28	C.H. Boehringer Sohn, 6507 Ingelheim	Verfahren und reagenz zur bestimmung von harnsaeure
JPS6091998A (ja) *	1983-10-27	1985-05-23	Yukio Shigeta	体液及び尿中のｄ―３―ヒドロキシ酪酸の測定法並びにそのための測定試薬
IT1199088B (it) *	1984-03-09	1988-12-30	Miles Italiana	Saggio di legame specifico mediante impiego di anti-g6pdh come marcante
US4668630A (en) *	1984-10-01	1987-05-26	Beckman Instruments, Inc.	Stabilized enzymatic composition
US4937047A (en) *	1986-04-01	1990-06-26	Konishiroku Photo Industry Co., Ltd.	Analytical element
JPS6394994A (ja) *	1986-10-09	1988-04-26	Fuji Photo Film Co Ltd	酸化型補酵素含有乾式分析要素
US5232947A (en) *	1988-07-04	1993-08-03	Meiji Seika Kaisha, Ltd.	Oxamic acid compounds and pharmaceutical composition for use in improvement of damaged cerebral functions of brain
AUPN200695A0 (en) *	1995-03-28	1995-04-27	Trace Scientific Pty Ltd	Transaminase reagent
US5998158A (en) *	1997-05-14	1999-12-07	Fuji Photo Film Co., Ltd.	Glucose free, stable dry analytical element for quantitative analysis of creatine kinase MB isozyme
DE69809740T2 (de) *	1998-09-22	2003-04-10	Fuji Photo Film Co., Ltd.	Trockenes analytisches Element zur quantitativen Analyse von Creatinkinase-MB-Isoenzym
TWI275795B (en) *	2001-02-14	2007-03-11	Sysmex Corp	Novel assay method
CN104603282A (zh) *	2012-08-31	2015-05-06	协和梅迪克斯株式会社	高密度脂蛋白中的胆固醇的测定方法
CN108315367B (zh) *	2018-03-19	2021-05-11	郑州四维健康管理有限公司	一种两步酶解法生产磷酸肌酸的方法
CN118483175B (zh) *	2024-05-16	2024-12-10	山东省医疗器械和药品包装检验研究院	一种自动测定抗凝牛血浆中菊粉含量的方法

Family Cites Families (4)

* Cited by examiner, † Cited by third party
Publication number	Priority date	Publication date	Assignee	Title
US3388044A (en) *	1965-12-07	1968-06-11	Warner Lambert Pharmaceutical	Process for differentiating the isoenzymes of lactic dehydrogenase
US3540984A (en) *	1966-06-30	1970-11-17	Calbiochem	Reagent material and method for creative kinase assay
DE1930059C3 (de) *	1969-06-13	1975-11-13	Boehringer Mannheim Gmbh	Stabilisiertes Nicotinamid-adenindinucleotid oder bzw. und Nicotinamld-adenin-dinucleotidphosphat
DE1940816C3 (de) *	1969-08-11	1974-01-17	Boehringer Mannheim Gmbh, 6800 Mannheim	Verfahren und diagnostisches Mittel zur enzymatischen Bestimmung von Glucose

1974
- 1974-04-29 US US05/465,039 patent/US3956069A/en not_active Expired - Lifetime
1975
- 1975-02-13 CA CA220,039A patent/CA1051326A/en not_active Expired
- 1975-04-18 JP JP50046631A patent/JPS5110996A/ja active Pending
- 1975-04-25 GB GB17394/75A patent/GB1491313A/en not_active Expired
- 1975-04-25 SE SE7504845A patent/SE405046B/xx unknown
- 1975-04-28 FR FR7513272A patent/FR2269080B1/fr not_active Expired
- 1975-04-28 DE DE19752518862 patent/DE2518862B2/de not_active Withdrawn
1978
- 1978-03-16 SE SE7803067A patent/SE420420B/sv unknown

Also Published As

Publication number	Publication date
US3956069A (en)	1976-05-11
SE405046B (sv)	1978-11-13
FR2269080B1 (2)	1978-06-30
SE7504845L (sv)	1975-10-30
GB1491313A (en)	1977-11-09
DE2518862B2 (de)	1977-12-15
SE420420B (sv)	1981-10-05
JPS5110996A (2)	1976-01-28
FR2269080A1 (2)	1975-11-21
SE7803067L (sv)	1978-03-16
DE2518862A1 (de)	1975-11-13

Publication	Publication Date	Title
CA1051326A (en)	1979-03-27	Method and reagent for reducing errors in enzymatic assays
EP0973938B1 (en)	2008-01-02	The use of nadph and nadh analogs in the measurement of enzyme activities and metabolites
US4242446A (en)	1980-12-30	Method for determining a substance in a biological fluid and reagent combination for use in the method
EP0034213B1 (en)	1984-11-07	A stabilized aqueous coenzyme solution for use in a clinical assay, a method of stabilizing a labile coenzyme in an aqueous clinical assay solution and a kit for use in said clinical assay
US6380380B1 (en)	2002-04-30	Use of nicotinamide adenine dinucleotide (NAD) and nicotinamide adenine dinucliotide phosphate (NADP) analogs to measure enzyme activities metabolites and substrates
US4271264A (en)	1981-06-02	Stabilized liquid enzyme and coenzyme compositions
Hess et al.	1974	Pyruvate kinase from yeast
US4241179A (en)	1980-12-23	Method for determining a transaminase in a biological fluid and reagent combination for use in the method
EP0712937A1 (en)	1996-05-22	Method of determining chloride ion
CA1062592A (en)	1979-09-18	Method for determination of creatine phosphokinase activity in body fluids
Benziman et al.	1972	Factors affecting hexose phosphorylation in Acetobacter xylinum
US5506113A (en)	1996-04-09	Measuring composition
US5250416A (en)	1993-10-05	Method for highly sensitive determination of NADH using kinase
US5817467A (en)	1998-10-06	Method for quantitatively determining creatinine kinase and a reagent therefor
Wakisaka et al.	1987	A rapid assay method for ammonia using glutamine synthetase from glutamate-producing bacteria
US5834227A (en)	1998-11-10	Kit for assaying creatine kinase
CA1175737A (en)	1984-10-09	Determination of creatine phosphokinase in body fluids
US3838010A (en)	1974-09-24	Photometric method for determining lactic acid dehydrogenase or glucose-6-phosphate dehydrogenase
CA1053548A (en)	1979-05-01	Method and reagent for reducing errors in enzymatic assays
JP2564295B2 (ja)	1996-12-18	ビタミンｅ欠乏症の検査のためのピルビン酸キナーゼ活性の測定方法
JPS6236199A (ja)	1987-02-17	カルシウムイオンの定量法
JPS59166099A (ja)	1984-09-19	Ａｔｐまたはｎｍｎの高感度測定法
JP3586737B2 (ja)	2004-11-10	生体物質の測定方法
JP3566976B2 (ja)	2004-09-15	生体成分中の測定対象の測定方法
Grassl	1974	Guanosine-5′-triphosphate and inosine-5′-triphosphate