CA1064395A - Culture de cellules et de virus - Google Patents

Culture de cellules et de virus

Info

Publication number: CA1064395A
Authority: CA; Canada
Prior art keywords: virus; cells; medium; vaccine; fetal
Prior art date: 1975-05-12
Legal status (The legal status is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the status listed.): Expired

Application number

CA249,860A

Other languages

English (en)

Inventor

Victor J. Cabasso

Current Assignee (The listed assignees may be inaccurate. Google has not performed a legal analysis and makes no representation or warranty as to the accuracy of the list.)

Bayer Corp

Original Assignee

Cutter Laboratories Inc

Priority date (The priority date is an assumption and is not a legal conclusion. Google has not performed a legal analysis and makes no representation as to the accuracy of the date listed.)

1975-05-12

Filing date

1976-04-08

Publication date

1979-10-16

1976-04-08 Application filed by Cutter Laboratories Inc filed Critical Cutter Laboratories Inc

1979-10-16 Application granted granted Critical

1979-10-16 Publication of CA1064395A publication Critical patent/CA1064395A/fr

Status Expired legal-status Critical Current

Links

241000700605 Viruses Species 0.000 title claims abstract description 46
241000282465 Canis Species 0.000 claims abstract description 26
210000005265 lung cell Anatomy 0.000 claims abstract description 19
229960005486 vaccine Drugs 0.000 claims abstract description 18
230000012010 growth Effects 0.000 claims abstract description 16
238000004113 cell culture Methods 0.000 claims abstract description 14
235000015097 nutrients Nutrition 0.000 claims abstract description 11
238000002360 preparation method Methods 0.000 claims abstract description 10
210000004027 cell Anatomy 0.000 claims description 72
238000000034 method Methods 0.000 claims description 25
239000002609 medium Substances 0.000 claims description 20
230000001605 fetal effect Effects 0.000 claims description 18
238000003306 harvesting Methods 0.000 claims description 13
239000001963 growth medium Substances 0.000 claims description 12
241000711798 Rabies lyssavirus Species 0.000 claims description 10
108091003079 Bovine Serum Albumin Proteins 0.000 claims description 7
230000029812 viral genome replication Effects 0.000 claims description 7
241000120506 Bluetongue virus Species 0.000 claims description 4
241000701083 Bovine alphaherpesvirus 1 Species 0.000 claims description 4
241000712003 Human respirovirus 3 Species 0.000 claims description 4
241000712083 Canine morbillivirus Species 0.000 claims description 3
210000000349 chromosome Anatomy 0.000 claims description 3
239000012091 fetal bovine serum Substances 0.000 claims description 3
241000283690 Bos taurus Species 0.000 claims description 2
229940000425 combination drug Drugs 0.000 claims description 2
229960003127 rabies vaccine Drugs 0.000 claims 3
208000000655 Distemper Diseases 0.000 claims 2
208000002606 Paramyxoviridae Infections Diseases 0.000 claims 2
208000003836 bluetongue Diseases 0.000 claims 2
208000014058 canine distemper Diseases 0.000 claims 2
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206010051497 Rhinotracheitis Diseases 0.000 claims 1
230000006978 adaptation Effects 0.000 claims 1
230000000694 effects Effects 0.000 claims 1
208000005562 infectious bovine rhinotracheitis Diseases 0.000 claims 1
230000003612 virological effect Effects 0.000 abstract description 7
239000000463 material Substances 0.000 abstract description 6
230000000890 antigenic effect Effects 0.000 abstract description 2
239000012530 fluid Substances 0.000 description 8
238000004519 manufacturing process Methods 0.000 description 8
238000012360 testing method Methods 0.000 description 6
239000000725 suspension Substances 0.000 description 5
210000001519 tissue Anatomy 0.000 description 5
UIIMBOGNXHQVGW-UHFFFAOYSA-M Sodium bicarbonate Chemical compound [Na+].OC([O-])=O UIIMBOGNXHQVGW-UHFFFAOYSA-M 0.000 description 4
239000012894 fetal calf serum Substances 0.000 description 4
238000011081 inoculation Methods 0.000 description 4
210000004072 lung Anatomy 0.000 description 4
UCSJYZPVAKXKNQ-HZYVHMACSA-N streptomycin Chemical compound CN[C@H]1[C@H](O)[C@@H](O)[C@H](CO)O[C@H]1O[C@@H]1[C@](C=O)(O)[C@H](C)O[C@H]1O[C@@H]1[C@@H](NC(N)=N)[C@H](O)[C@@H](NC(N)=N)[C@H](O)[C@H]1O UCSJYZPVAKXKNQ-HZYVHMACSA-N 0.000 description 4
230000001461 cytolytic effect Effects 0.000 description 3
239000000203 mixture Substances 0.000 description 3
239000000243 solution Substances 0.000 description 3
238000004448 titration Methods 0.000 description 3
JKMHFZQWWAIEOD-UHFFFAOYSA-N 2-[4-(2-hydroxyethyl)piperazin-1-yl]ethanesulfonic acid Chemical compound OCC[NH+]1CCN(CCS([O-])(=O)=O)CC1 JKMHFZQWWAIEOD-UHFFFAOYSA-N 0.000 description 2
APKFDSVGJQXUKY-KKGHZKTASA-N Amphotericin-B Natural products O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1C=CC=CC=CC=CC=CC=CC=C[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-KKGHZKTASA-N 0.000 description 2
241000894006 Bacteria Species 0.000 description 2
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241000233866 Fungi Species 0.000 description 2
239000007995 HEPES buffer Substances 0.000 description 2
241000204031 Mycoplasma Species 0.000 description 2
241000702259 Orbivirus Species 0.000 description 2
229930182555 Penicillin Natural products 0.000 description 2
JGSARLDLIJGVTE-MBNYWOFBSA-N Penicillin G Chemical compound N([C@H]1[C@H]2SC([C@@H](N2C1=O)C(O)=O)(C)C)C(=O)CC1=CC=CC=C1 JGSARLDLIJGVTE-MBNYWOFBSA-N 0.000 description 2
APKFDSVGJQXUKY-INPOYWNPSA-N amphotericin B Chemical compound O[C@H]1[C@@H](N)[C@H](O)[C@@H](C)O[C@H]1O[C@H]1/C=C/C=C/C=C/C=C/C=C/C=C/C=C/[C@H](C)[C@@H](O)[C@@H](C)[C@H](C)OC(=O)C[C@H](O)C[C@H](O)CC[C@@H](O)[C@H](O)C[C@H](O)C[C@](O)(C[C@H](O)[C@H]2C(O)=O)O[C@H]2C1 APKFDSVGJQXUKY-INPOYWNPSA-N 0.000 description 2
229960003942 amphotericin b Drugs 0.000 description 2
239000003242 anti bacterial agent Substances 0.000 description 2
229940088710 antibiotic agent Drugs 0.000 description 2
229960000074 biopharmaceutical Drugs 0.000 description 2
230000015572 biosynthetic process Effects 0.000 description 2
210000003837 chick embryo Anatomy 0.000 description 2
230000007850 degeneration Effects 0.000 description 2
210000001840 diploid cell Anatomy 0.000 description 2
238000009472 formulation Methods 0.000 description 2
230000000855 fungicidal effect Effects 0.000 description 2
239000000417 fungicide Substances 0.000 description 2
238000011534 incubation Methods 0.000 description 2
208000015181 infectious disease Diseases 0.000 description 2
229940049954 penicillin Drugs 0.000 description 2
239000002356 single layer Substances 0.000 description 2
229910000030 sodium bicarbonate Inorganic materials 0.000 description 2
235000017557 sodium bicarbonate Nutrition 0.000 description 2
238000001179 sorption measurement Methods 0.000 description 2
229960005322 streptomycin Drugs 0.000 description 2
241001529453 unidentified herpesvirus Species 0.000 description 2
241001600407 Aphis <genus> Species 0.000 description 1
BVKZGUZCCUSVTD-UHFFFAOYSA-M Bicarbonate Chemical compound OC([O-])=O BVKZGUZCCUSVTD-UHFFFAOYSA-M 0.000 description 1
208000005623 Carcinogenesis Diseases 0.000 description 1
239000012594 Earle’s Balanced Salt Solution Substances 0.000 description 1
206010056740 Genital discharge Diseases 0.000 description 1
241001465754 Metazoa Species 0.000 description 1
229930193140 Neomycin Natural products 0.000 description 1
108010040201 Polymyxins Proteins 0.000 description 1
206010037742 Rabies Diseases 0.000 description 1
240000004808 Saccharomyces cerevisiae Species 0.000 description 1
235000017276 Salvia Nutrition 0.000 description 1
241001072909 Salvia Species 0.000 description 1
102000004142 Trypsin Human genes 0.000 description 1
108090000631 Trypsin Proteins 0.000 description 1
230000005856 abnormality Effects 0.000 description 1
238000004458 analytical method Methods 0.000 description 1
210000004102 animal cell Anatomy 0.000 description 1
239000000427 antigen Substances 0.000 description 1
102000036639 antigens Human genes 0.000 description 1
108091007433 antigens Proteins 0.000 description 1
230000002238 attenuated effect Effects 0.000 description 1
230000001580 bacterial effect Effects 0.000 description 1
230000003115 biocidal effect Effects 0.000 description 1
239000006172 buffering agent Substances 0.000 description 1
230000036952 cancer formation Effects 0.000 description 1
231100000504 carcinogenesis Toxicity 0.000 description 1
230000010261 cell growth Effects 0.000 description 1
239000013553 cell monolayer Substances 0.000 description 1
239000006285 cell suspension Substances 0.000 description 1
238000012512 characterization method Methods 0.000 description 1
239000003795 chemical substances by application Substances 0.000 description 1
239000000356 contaminant Substances 0.000 description 1
238000011109 contamination Methods 0.000 description 1
230000000120 cytopathologic effect Effects 0.000 description 1
230000003111 delayed effect Effects 0.000 description 1
239000012895 dilution Substances 0.000 description 1
238000010790 dilution Methods 0.000 description 1
201000010099 disease Diseases 0.000 description 1
208000037265 diseases, disorders, signs and symptoms Diseases 0.000 description 1
239000002552 dosage form Substances 0.000 description 1
230000007717 exclusion Effects 0.000 description 1
210000003754 fetus Anatomy 0.000 description 1
239000012737 fresh medium Substances 0.000 description 1
238000007429 general method Methods 0.000 description 1
239000011521 glass Substances 0.000 description 1
230000003053 immunization Effects 0.000 description 1
238000002649 immunization Methods 0.000 description 1
239000002054 inoculum Substances 0.000 description 1
238000007689 inspection Methods 0.000 description 1
210000003292 kidney cell Anatomy 0.000 description 1
238000012423 maintenance Methods 0.000 description 1
230000014759 maintenance of location Effects 0.000 description 1
239000012569 microbial contaminant Substances 0.000 description 1
230000000877 morphologic effect Effects 0.000 description 1
229960004927 neomycin Drugs 0.000 description 1
231100001222 nononcogenic Toxicity 0.000 description 1
231100001221 nontumorigenic Toxicity 0.000 description 1
210000000056 organ Anatomy 0.000 description 1
238000007911 parenteral administration Methods 0.000 description 1
230000035935 pregnancy Effects 0.000 description 1
235000002020 sage Nutrition 0.000 description 1
150000003839 salts Chemical class 0.000 description 1
241000894007 species Species 0.000 description 1
239000003104 tissue culture media Substances 0.000 description 1
239000012588 trypsin Substances 0.000 description 1
230000005740 tumor formation Effects 0.000 description 1

Classifications

- A—HUMAN NECESSITIES
- A61—MEDICAL OR VETERINARY SCIENCE; HYGIENE
- A61K—PREPARATIONS FOR MEDICAL, DENTAL OR TOILETRY PURPOSES
- A61K39/00—Medicinal preparations containing antigens or antibodies
- A61K39/12—Viral antigens
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
- C12N2710/16734—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2710/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsDNA viruses
- C12N2710/00011—Details
- C12N2710/16011—Herpesviridae
- C12N2710/16711—Varicellovirus, e.g. human herpesvirus 3, Varicella Zoster, pseudorabies
- C12N2710/16751—Methods of production or purification of viral material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/12011—Reoviridae
- C12N2720/12111—Orbivirus, e.g. bluetongue virus
- C12N2720/12134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2720/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA dsRNA viruses
- C12N2720/00011—Details
- C12N2720/12011—Reoviridae
- C12N2720/12111—Orbivirus, e.g. bluetongue virus
- C12N2720/12151—Methods of production or purification of viral material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
- C12N2760/18434—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18411—Morbillivirus, e.g. Measles virus, canine distemper
- C12N2760/18451—Methods of production or purification of viral material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18634—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/18011—Paramyxoviridae
- C12N2760/18611—Respirovirus, e.g. Bovine, human parainfluenza 1,3
- C12N2760/18651—Methods of production or purification of viral material
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
- C12N2760/20134—Use of virus or viral component as vaccine, e.g. live-attenuated or inactivated virus, VLP, viral protein
- C—CHEMISTRY; METALLURGY
- C12—BIOCHEMISTRY; BEER; SPIRITS; WINE; VINEGAR; MICROBIOLOGY; ENZYMOLOGY; MUTATION OR GENETIC ENGINEERING
- C12N—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA
- C12N2760/00—MICROORGANISMS OR ENZYMES; COMPOSITIONS THEREOF; PROPAGATING, PRESERVING, OR MAINTAINING MICROORGANISMS; MUTATION OR GENETIC ENGINEERING; CULTURE MEDIA ssRNA viruses negative-sense
- C12N2760/00011—Details
- C12N2760/20011—Rhabdoviridae
- C12N2760/20111—Lyssavirus, e.g. rabies virus
- C12N2760/20151—Methods of production or purification of viral material

Landscapes

Health & Medical Sciences (AREA)
Life Sciences & Earth Sciences (AREA)
Pharmacology & Pharmacy (AREA)
Epidemiology (AREA)
Immunology (AREA)
Medicinal Chemistry (AREA)
Microbiology (AREA)
Mycology (AREA)
Virology (AREA)
Chemical & Material Sciences (AREA)
Animal Behavior & Ethology (AREA)
General Health & Medical Sciences (AREA)
Public Health (AREA)
Veterinary Medicine (AREA)
Medicines Containing Antibodies Or Antigens For Use As Internal Diagnostic Agents (AREA)
Micro-Organisms Or Cultivation Processes Thereof (AREA)

CA249,860A 1975-05-12 1976-04-08 Culture de cellules et de virus Expired CA1064395A (fr)

Applications Claiming Priority (1)

Application Number	Priority Date	Filing Date	Title
US57652775A	1975-05-12	1975-05-12

Publications (1)

Publication Number	Publication Date
CA1064395A true CA1064395A (fr)	1979-10-16

Family

ID=24304804

Family Applications (1)

Application Number	Title	Priority Date	Filing Date
CA249,860A Expired CA1064395A (fr)	1975-05-12	1976-04-08	Culture de cellules et de virus

Country Status (6)

Country	Link
AR (1)	AR212170A1 (fr)
AU (1)	AU505075B2 (fr)
CA (1)	CA1064395A (fr)
ES (1)	ES446396A1 (fr)
GB (1)	GB1501556A (fr)
NZ (1)	NZ180813A (fr)

1976
- 1976-03-26 ES ES446396A patent/ES446396A1/es not_active Expired
- 1976-04-08 CA CA249,860A patent/CA1064395A/fr not_active Expired
- 1976-04-09 GB GB1463276A patent/GB1501556A/en not_active Expired
- 1976-05-10 NZ NZ18081376A patent/NZ180813A/xx unknown
- 1976-05-12 AR AR26325876A patent/AR212170A1/es active
- 1976-05-12 AU AU13865/76A patent/AU505075B2/en not_active Expired

Also Published As

Publication number	Publication date
AU505075B2 (en)	1979-11-08
ES446396A1 (es)	1977-10-16
AU1386576A (en)	1977-11-17
GB1501556A (en)	1978-02-15
AR212170A1 (es)	1978-05-31
NZ180813A (en)	1979-03-16

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CA1122527A (fr)	1982-04-27	Production d'un vaccin contre l'influenza par culture de cellules en milieu liquide
EP0125245B1 (fr)	1987-05-06	Propagation du virus de l'enterite hemorragique dans des cellules de dinde
JPS62115279A (ja)	1987-05-26	ネコ鼻腔気管炎ウイルスの大量生産方法
EP0011864B1 (fr)	1982-05-19	Souche atténuée du virus de la péritonite infectieuse des félins, procédé pour sa préparation et vaccin la contenant
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US20180237743A1 (en)	2018-08-23	Production of avian embryo cells
JPH0998778A (ja)	1997-04-15	マレク病ワクチン
US4021302A (en)	1977-05-03	Cell cultures
US5006335A (en)	1991-04-09	Live vaccine against mumps and process for obtaining thereof
CA2168686A1 (fr)	1996-08-16	Methode de croissance d'un virus
Mifune et al.	1979	Susceptibility of various cell lines to rabies virus
US4358438A (en)	1982-11-09	Novel polyvalent virus vaccine against rabies and canine distemper
BE859178A (fr)	1978-01-16	Souche de cellules et culture de virus
SU1360193A1 (ru)	1989-08-07	Штамм культивируемых клеток почки эмбриона человека дл накоплени и титровани вируса клещевого энцефалита